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&nbsp;\u4f2f\u4e50\u7684\u8f6c\u819c\u69fd\u53d1\u70ed\u5389\u5bb3\uff0c\u9700\u8981\u63a7\u5236\u53d1\u70ed\uff1b\u6cd5\u739b\u897f\u4e9a\u7684\u8f6c\u819c\u69fd\uff0c\u70ed\u91cf\u63a7\u5236\u6bd4\u8f83\u597d\uff0c\u53ef\u4ee5\u505a\u5230\u4e00\u70b9\u90fd\u4e0d\u53d1\u70ed\u3002\u2464 \u534a\u5e72\u8f6c\u7684\u4e09\u660e\u6cbb\u8981\u4fdd\u6301\u6e7f\u6da6\uff0c\u4f46\u662f\u4e09\u660e\u6cbb\u5468\u56f4\u7684\u91d1\u5c5e\u677f\u8981\u4fdd\u6301\u5e72\u71e5\u3002\u2465 \u534a\u5e72\u8f6c\u7684\u6761\u4ef6\u662f\u7528\u6052\u538b 20V\uff0c20min-1h\u3002\u5982\u679c\u86cb\u767d\u5927\u7684\u53ef\u4ee5\u5ef6\u957f\u8f6c\u819c\u65f6\u95f4\u3002\u2466 \u6e7f\u8f6c\u80fd\u8f6c\u7684\u86cb\u767d\u5927\u5c0f\u8303\u56f4\u975e\u5e38\u5bbd\uff0c\u666e\u901a\u7684\u534a\u5e72\u8f6c\u9002\u5408\u8f6c 30-120KD 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\u4e0e\u86cb\u767d\u8d28\u7ed3\u5408\u540e\uff0c\u80fd\u4f7f\u86cb\u767d\u66f4\u5bb9\u6613\u4ece\u80f6\u4e0a\u8f6c\u51fa\u6765\uff0c\u4f46\u53c8\u80fd\u963b\u6b62\u86cb\u767d\u4e0e\u819c\u7ed3\u5408\uff0c\u5c24\u5176\u662f\u86cb\u767d\u4e0e NC \u819c\u7684\u7ed3\u5408\u3002\u56e0\u6b64\uff0c\u5927\u86cb\u767d\u4e0d\u5bb9\u6613\u8f6c\u51fa\uff0c\u8981\u989d\u5916\u52a0 SDS\uff1b\u5c0f\u86cb\u767d\u9760 PAGE \u80f6\u91cc\u7684 SDS \u5c31\u8db3\u591f\u8f6c\u51fa\u4e86\u3002\u8fc7\u591a\u7684 SDS \u8fd8\u4f1a\u5f71\u54cd\u7535\u6d41\uff0c\u589e\u52a0\u53d1\u70ed\uff0c\u4ece\u800c\u5f71\u54cd\u67d0\u4e9b\u86cb\u767d\u7684\u6297\u539f\u6027\u3002\u6240\u4ee5\u9700\u8981\u6839\u636e\u60c5\u51b5\u63a7\u5236 SDS \u7684\u542b\u91cf\u3002\u7532\u9187\u80fd\u7834\u574f SDS \u4e0e\u86cb\u767d\u7684\u7ed3\u5408\uff0c\u4fc3\u4f7f\u86cb\u767d\u4e0e\u819c\u7ed3\u5408\uff0c\u4f46\u4f1a\u51cf\u5c0f PAGE \u80f6\u7684\u5b54\u5f84\uff0c\u4f7f\u4e00\u4e9b\u86cb\u767d\u53d1\u751f\u6c89\u6dc0\uff0c\u4ece\u800c\u5f71\u54cd\u5b9e\u9a8c\u7ed3\u679c\uff0c\u6240\u4ee5\u4e5f\u5f97\u4e25\u683c\u63a7\u5236\u91cf\u30024. \u6297\u4f53\u6742\u4ea41&gt; \u5c01\u95ed\uff1a\u4f7f\u7528 5%\u8131\u8102\u725b\u5976 \u6216 5% BSA\uff08\u7528 TBST \u6eb6\u89e3\uff09\u4f5c\u4e3a\u5c01\u95ed\u6db2\uff0c\u5c01\u95ed\u6761\u4ef6\uff1a\u5ba4\u6e29 1h\uff0c\u6216 4\u2103\u8fc7\u591c\u3002\uff08\u76ee\u7684\u86cb\u767d\u8f6c\u819c\u540e\uff0c\u4f46\u819c\u4e0a\u8fd8\u6709\u5f88\u591a\u5176\u4ed6\u86cb\u767d\uff0c\u56e0\u4e3a\u8dd1\u80f6\u7528\u7684\u662f\u603b\u86cb\u767d\uff0c\u5176\u4ed6\u975e\u76ee\u7684\u86cb\u767d\u4e5f\u4f1a\u968f\u7740\u8f6c\u79fb\u5230\u819c\u4e0a\uff0c\u8fd9\u6837\u5c31\u4f1a\u66b4\u9732\u51fa\u5f88\u591a\u6297\u4f53\u7ed3\u5408\u4f4d\u70b9\uff0c\u56e0\u6b64\u9700\u8981\u5728\u6297\u4f53\u6742\u4ea4\u524d\u5c01\u95ed\u819c\u3002\uff09&nbsp; &nbsp; BSA\uff1a\u4e00\u822c\u662f\u505a\u86cb\u767d\u78f7\u9178\u5316\u4fee\u9970\u68c0\u6d4b\u65f6\u7528\uff0c\u56e0\u4e3a\u725b\u5976\u4e2d\u542b\u6709\u4e30\u5bcc\u7684\u78f7\u9178&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; \u9176\uff0c\u4f1a\u6539\u53d8\u86cb\u767d\u78f7\u9178\u5316\u4fee\u9970\u7684\u72b6\u6001\u3002\u6709\u65f6\uff0c\u5c01\u95ed\u6db2\u914d\u65b9\u548c\u5c01\u95ed\u6761\u4ef6\u4e5f\u9700\u8981&nbsp; &nbsp; &nbsp; \u6539\u53d8\uff0c\u7279\u522b\u662f\u5927\u86cb\u767d\uff0c5%\u8131\u8102\u725b\u5976\u5c01\u95ed\u4e00\u5c0f\u65f6\u4e0d\u591f\uff1b\u6709\u7684\u86cb\u767d\u53ef\u80fd\u725b\u5976\u548c&nbsp; &nbsp; &nbsp; BSA \u90fd\u4e0d\u80fd\u7528\uff0c\u53ef\u4ee5\u7528\u660e\u80f6\u5c01\u95ed\u6216\u4e0d\u5c01\u95ed\u76f4\u63a5\u4e0a\u6297\u4f53\u30022&gt; \u4e00\u6297\u5b75\u80b2\uff1a\u4f7f\u7528\u5c01\u95ed\u6db2\u7a00\u91ca\u4e00\u6297\uff0c4\u2103\u8fc7\u591c\uff0c\u6216\u5ba4\u6e29\u6216 37\u2103 1-X h\u30023&gt; TBST \u6d17\u819c\uff0c\u5ba4\u6e29\uff0c5min \u00d74 \u6b21\u30024&gt; \u4e8c\u6297\u5b75\u80b2\uff1a\u5ba4\u6e29 1h\u30025&gt; TBST \u6d17\u819c\uff0c\u5ba4\u6e29\uff0c5min \u00d78-12 \u6b21\u30026&gt; ECL \u663e\u8272\uff0cX \u5149\u7247\u538b\u7247\u6216\u626b\u819c\u3002TBS \u914d\u65b9\uff1a1L TBS \u4e2d\u52a0\u5165 1ml Tween 20 \u5c31\u662f TBST\u3002Tween 20\uff1a\u4e00\u79cd\u975e\u79bb\u5b50\u578b\u53bb\u6c61\u5242\uff0c\u6709\u590d\u6027\u6297\u539f\u7684\u4f5c\u7528\uff0c\u53ef\u4ee5\u63d0\u9ad8\u6297\u4f53\u7279\u5f02\u6027\u8bc6\u522b\u80fd\u529b\uff1b\u63d0\u9ad8\u5c01\u95ed\u6548\u679c\uff0c\u7528\u60f0\u6027\u86cb\u767d\u8d28\u6216\u975e\u79bb\u5b50\u53bb\u6c61\u5242\u5c01\u95ed\u819c\u4e0a\u7684\u672a\u7ed3\u5408\u4f4d\u70b9\uff0c\u53ef\u4ee5\u964d\u4f4e\u6297\u4f53\u7684\u975e\u7279\u5f02\u6027\u7ed3\u5408\uff1b\u4e73\u5316\u86cb\u767d\u65f6\u4e0d\u4f1a\u7834\u574f\u86cb\u767d\u7ed3\u6784\u30025. \u56fe\u50cf\u5206\u6790\u4f7f\u7528 Gel-Pro Analyzer \u6216 ImageJ\uff08\u63a8\u8350\uff09\uff1b\u540e\u9762\u4f1a\u5355\u72ec\u6574\u7406\u8fd9\u65b9\u9762\u5185\u5bb9\uff1b6. \u5e38\u89c1\u95ee\u9898\u5206\u67901) \u975e\u7279\u5f02\u6761\u5e26\uff1a\u6297\u4f53\u6d53\u5ea6\u8fc7\u9ad8\uff1b&nbsp;\u89e3\u51b3\u65b9\u6cd5\uff1a\u964d\u4f4e\u6297\u4f53\u6d53\u5ea6\uff1b\u5c01\u95ed\u4e0d\u8db3\uff1b\u89e3\u51b3\u65b9\u6cd5\uff1a&nbsp; &nbsp; \u63d0\u9ad8\u5c01\u95ed\u6db2\u6d53\u5ea6\uff0c\u5982\u4ece 5% \u63d0\u9ad8\u5230 7%\uff1b&nbsp; &nbsp; \u589e\u52a0\u5c01\u95ed\u65f6\u95f4\u548c\/\u6216\u6e29\u5ea6\uff1b&nbsp; &nbsp; \u5411\u5c01\u95ed\u7f13\u51b2\u6eb6\u6db2\u4e2d\u52a0\u5165 0.05% \u7684 Tween 20\uff1b&nbsp; &nbsp; \u6297\u4f53\u7a00\u91ca\u6db2\u91c7\u7528\u76f8\u540c\u7684\u5c01\u95ed\u6db2\uff1bSDS \u4f7f\u6297\u4f53\u548c\u86cb\u767d\u6761\u5e26\u53d1\u751f\u975e\u7279\u5f02\u6027\u7ed3\u5408\uff1a&nbsp;\u89e3\u51b3\u65b9\u6cd5\uff1a&nbsp; &nbsp; \u8f6c\u819c\u540e\u6d17\u6da4\u5370\u8ff9\u819c\uff1b&nbsp; &nbsp; \u5728\u514d\u75ab\u68c0\u6d4b\u8fc7\u7a0b\u4e2d\u4e0d\u8981\u4f7f\u7528 SDS\uff1b\u6297\u4f53\u8d28\u91cf\uff1a\u5c1d\u8bd5\u4f7f\u7528\u4e0d\u540c\u7684\u6297\u4f53\uff1b2) \u6761\u5e26\u5f31\u6216\u65e0\u6761\u5e26\uff1a\u6297\u4f53\u95ee\u9898\uff0c\u4f4e\u6d3b\u6027\u6216\u4f4e\u6d53\u5ea6\u3001\u4f4e\u4eb2\u548c\u529b\u7684\u4e00\u6297\uff0c\u9519\u914d\u7684\u4e00\u6297\u548c\u4e8c\u6297\uff1a&nbsp; &nbsp;&nbsp;\u89e3\u51b3\u65b9\u6cd5\uff1a&nbsp; &nbsp; \u63d0\u9ad8\u6297\u4f53\u6d53\u5ea6\uff1b&nbsp; &nbsp; \u6297\u4f53\u53ef\u80fd\u5df2\u7ecf\u4e27\u5931\u6d3b\u6027\uff0c\u8fdb\u884c\u6591\u70b9\u6742\u4ea4\u4ee5\u786e\u5b9a\u5176\u6d3b\u6027\u548c\u6700\u4f73\u6d53\u5ea6\uff1b&nbsp; &nbsp; \u6d4b\u8bd5\u4e0d\u540c\u4e00\u6297\u548c\/\u6216\u4e00\u6297-\u4e8c\u6297\u7ec4\u5408\uff1b\u6297\u539f\u6297\u4f53\u53cd\u5e94\u4e0d\u8db3\uff1a\u89e3\u51b3\u65b9\u6cd5\uff1a&nbsp; &nbsp; \u589e\u52a0\u51dd\u80f6\u4e2d\u6bcf\u4e2a\u6837\u54c1\u7684\u603b\u86cb\u767d\u4e0a\u6837\u91cf\uff1b&nbsp; &nbsp; \u68c0\u67e5\u6837\u54c1\u7684\u5b8c\u6574\u6027\uff0c\u786e\u4fdd\u86cb\u767d\u672a\u964d\u89e3\uff1b\u6297\u539f\u88ab\u5c01\u95ed\u6db2\u5c01\u95ed\uff1a&nbsp; &nbsp; \u89e3\u51b3\u65b9\u6cd5\uff1a&nbsp; &nbsp; \u5c1d\u8bd5\u4e0d\u540c\u7684\u5c01\u95ed\u6db2 \uff08\u6bd4\u5982\uff0c\u5728\u68c0\u6d4b\u78f7\u9178\u5316\u86cb\u767d\u65f6\u907f\u514d\u4f7f\u7528\u725b\u5976\uff09\uff1b&nbsp; &nbsp; \u4f18\u5316\u5c01\u95ed\u6db2\u6d53\u5ea6\uff0c\u5efa\u8bae\u4f7f\u7528\u6d53\u5ea6\u4e3a 3\u20135% \u7684 BSA \u6216\u8131\u8102\u5976\u7c89\uff1b\u5316\u5b66\u53d1\u5149\u5e95\u7269\u6027\u80fd\u4e0d\u4f73\uff1a&nbsp; &nbsp;&nbsp;&nbsp;\u589e\u52a0\u5e95\u7269\u5b75\u80b2\u65f6\u95f4\uff1b&nbsp; &nbsp; &nbsp;\u51c6\u5907\u5c11\u91cf\u5de5\u4f5c\u6db2\u4ee5\u786e\u5b9a\u5e95\u7269\u662f\u5426\u5df2\u7ecf\u4e27\u5931\u6d3b\u6027\uff0c\u6697\u5ba4\u5185\uff0c\u5728\u5e95\u7269\u5de5\u4f5c\u6db2\u4e2d\u52a0\u5165\u5c11\u91cf&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;HPR \u5076\u8054\u7269\u5219\u5e94\u8be5\u4f1a\u51fa\u73b0\u84dd\u5149\u3002\u5982\u679c\u6ca1\u6709\u7684\u8bdd\uff0c\u5fc5\u5b9a\u662f\u5e95\u7269\u6216HRP \u5076\u8054\u7269\u5df2\u4e27\u5931\u6d3b&nbsp; &nbsp; &nbsp; &nbsp;\u6027\uff1b&nbsp; &nbsp; &nbsp;\u786e\u4fdd\u5e95\u7269\u8bd5\u5242\u76d2\u4e2d\u7684\u4e24\u4e2a\u74f6\u5b50\u4e4b\u95f4\u6ca1\u6709\u53d1\u751f\u4ea4\u53c9\u6c61\u67d3\u3002\u4e24\u79cd\u8bd5\u5242\u4e4b\u95f4\u5982\u679c\u53d1\u751f\u4ea4\u53c9&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; \u6c61\u67d3\uff0c\u5219\u4f1a\u5f15\u8d77\u8bd5\u5242\u6d3b\u6027\u4e0b\u964d\uff1b&nbsp; &nbsp; &nbsp;\u53e0\u6c2e\u5316\u7269\u662f HRP \u7684\u6291\u5236\u5242\uff0c\u4e0d\u8981\u5728\u4e8c\u6297\u7f13\u51b2\u6db2\u4e2d\u4f7f\u7528\u53e0\u6c2e\u5316\u7269\uff1b\u5370\u8ff9\u819c\u4e0a\u6297\u4f53\u53bb\u9664\u548c\u518d\u6742\u4ea4\uff1a&nbsp; &nbsp; \u4f18\u5316\u6297\u4f53\u53bb\u9664\u6761\u4ef6\uff1b&nbsp; &nbsp; \u4ec5\u5728\u5fc5\u8981\u65f6\u8fdb\u884c\u518d\u6742\u4ea4\u68c0\u6d4b\uff1b&nbsp; &nbsp; \u907f\u514d\u5bf9\u540c\u4e00\u5370\u8ff9\u819c\u8fdb\u884c\u591a\u6b21\u91cd\u590d\u518d\u6742\u4ea4\uff1b\u8f6c\u819c\u4e0d\u8db3\uff1a&nbsp; &nbsp; \u8f6c\u819c\u65f6\u95f4\u8fc7\u77ed\uff0c\u5982\u679c\u4f7f\u7528\u6e7f\u8f6c\u6cd5\uff0c\u5219\u9700\u589e\u52a0\u8f6c\u819c\u65f6\u95f4\uff1b&nbsp; &nbsp; \u8f6c\u819c\u7535\u573a\u8fc7\u5f31\uff0c\u589e\u52a0\u8f6c\u819c\u7535\u538b\u6216\u7535\u6d41\uff1b&nbsp; &nbsp; \u8f6c\u819c\u6db2\u672a\u4f18\u5316\uff0c\u5728\u8f6c\u819c\u6db2\u4e2d\u52a0\u5165 0.01-0.05%\u7684 SDS\uff1b&nbsp; &nbsp; \u5c06\u8f6c\u819c\u6db2\u4e2d\u7684\u7532\u9187\u6d53\u5ea6\u51cf\u5c11\u81f3 5% \u6216\u66f4\u5c11\uff1b&nbsp; &nbsp; \u51dd\u80f6\u9009\u62e9\u4e0d\u6070\u5f53\uff0c\u4f7f\u7528\u66f4\u4f4e\u767e\u5206\u6bd4\u7684\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\uff1b&nbsp; &nbsp; \u5728\u8f6c\u819c\u540e\u4f7f\u7528\u4e3d\u6625\u7ea2\u67d3\u6db2\u67d3\u819c\uff0c\u8003\u9a6c\u65af\u4eae\u84dd\u67d3\u6db2\u67d3\u80f6\uff0c\u786e\u8ba4\u8f6c\u79fb\u6548\u7387\uff0c\u5e76\u5728\u6b64\u57fa\u7840\u4e0a&nbsp; &nbsp; &nbsp; \u4f18\u5316\u8f6c\u819c\u6761\u4ef6\uff1b\u8f6c\u8fc7\u4e86\uff1a&nbsp; &nbsp; \u8f6c\u819c\u65f6\u95f4\u8fc7\u957f\uff0c\u51cf\u5c11\u65f6\u95f4\uff1b&nbsp; &nbsp; \u8f6c\u819c\u7535\u573a\u8fc7\u5f3a\uff0c\u964d\u4f4e\u8f6c\u819c\u7535\u538b\u6216\u7535\u6d41\uff1b&nbsp; &nbsp; \u8f6c\u819c\u6db2\u672a\u4f18\u5316\uff0c\u7ec4\u88c5\u201c\u4e09\u660e\u6cbb\u201d\u4e4b\u524d\uff0c\u5728\u8f6c\u819c\u6db2\u4e2d\u5e73\u8861\u51dd\u80f6 20 \u5206\u949f\u4ee5\u964d\u4f4e\u51dd\u80f6\u4e2d SDS &nbsp;&nbsp; &nbsp; \u6d53\u5ea6\uff0c\u4ece\u800c\u907f\u514d\u5c0f\u5206\u5b50\u91cf\u86cb\u767d\u7684\u8fc7\u5ea6\u8f6c\u79fb\uff1b\u3002&nbsp; &nbsp; \u5c06\u8f6c\u819c\u6db2\u4e2d\u7684\u7532\u9187\u6d53\u5ea6\u589e\u52a0\u81f3 40%\uff1b&nbsp; &nbsp; \u51dd\u80f6\u9009\u62e9\u4e0d\u6070\u5f53\uff0c\u4f7f\u7528\u66f4\u9ad8\u767e\u5206\u6bd4\u7684\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\uff1b&nbsp; &nbsp; \u5f53\u76ee\u6807\u86cb\u767d\u5206\u5b50\u91cf\u5c0f\u4e8e 10 KD \u65f6\u4f7f\u7528 Tris\/tricine \u51dd\u80f6\u3002\u4f7f\u7528 0.2 \u03bcm \u7684\u819c\u89e3\u51b3\u5c0f\u86cb&nbsp; &nbsp; &nbsp; &nbsp;\u767d\u8f6c\u5370\u8fc7\u5ea6\u7684\u60c5\u51b5\uff1b&nbsp; &nbsp; \u5c0f\u86cb\u767d\u4f1a\u7a7f\u8fc7\u5927\u5b54\u5f84\u7684\u5370\u8ff9\u819c\uff0c\u5728 0.45\u03bcm \u7684\u819c\u540e\u53e0\u52a0 0.2 \u03bcm \u7684\u819c\u68c0\u6d4b\u5c0f\u86cb\u767d\u8f6c\u5370&nbsp; &nbsp; &nbsp; \u8fc7\u5ea6\u60c5\u51b5\uff1b3) \u4fe1\u53f7\u8fc7\u9971\u548c\uff1a\u4e2d\u7a7a\u6761\u5e26\uff1a&nbsp; &nbsp;\u4e0a\u6837\u91cf\u8fc7\u9ad8\uff0c\u964d\u4f4e\u6bcf\u4e2a\u6837\u54c1\u7684\u603b\u86cb\u767d\u4e0a\u6837\u91cf&nbsp; &nbsp; \u6297\u4f53\u8fc7\u591a\uff0c\u964d\u4f4e\u4e00\u6297\u548c\/\u6216\u4e8c\u6297\u6d53\u5ea6&nbsp; &nbsp; \u5316\u5b66\u53d1\u5149\u5e95\u7269\u8fc7\u7075\u654f\uff0c\u66f4\u6362\u4f7f\u7528\u7075\u654f\u5ea6\u8f83\u4f4e\u7684\u5316\u5b66\u53d1\u5149\u5e95\u7269\uff1b\u6761\u5e26\u8fc7\u6d53\u4ee5\u81f3\u4e8e\u6761\u5e26\u7c98\u8fde\u5728\u4e00\u8d77\uff1a&nbsp; &nbsp; \u6837\u54c1\u4e0a\u6837\u91cf\u8fc7\u5927\uff0c\u964d\u4f4e\u6bcf\u4e2a\u6837\u54c1\u7684\u603b\u86cb\u767d\u4e0a\u6837\u91cf\uff1b&nbsp; &nbsp; \u6297\u4f53\u8fc7\u591a\uff0c\u964d\u4f4e\u4e00\u6297\u548c\/\u6216\u4e8c\u6297\u6d53\u5ea6\uff1b4) \u6761\u5e26\u4e0a\u6709\u6c14\u6ce1\uff1a\u201c\u4e09\u660e\u6cbb\u201d\u7ec4\u88c5\u4e0d\u6070\u5f53\uff1a&nbsp; &nbsp; &nbsp;\u5728\u7ec4\u88c5 \u201c\u4e09\u660e\u6cbb\u201d\u7684\u65f6\u5019\u4f7f\u7528\u66f4\u591a\u7684\u8f6c\u819c\u7f13\u51b2\u6db2\uff1b&nbsp; &nbsp; &nbsp;\u7ec4\u88c5\u201c\u4e09\u660e\u6cbb\u201d\u7684\u65f6\u5019\u78be\u6389\u4e86\u51dd\u80f6\u548c\u819c\u4e4b\u95f4\u6240\u6709\u7684\u6c14\u6ce1\uff1b&nbsp; &nbsp; &nbsp;\u5728\u6297\u4f53\u5b75\u80b2\u524d\u7528\u4e3d\u6625\u7ea2\u67d3\u6db2 \u67d3\u819c\uff0c\u4ee5\u68c0\u67e5\u8f6c\u819c\u8d28\u91cf\uff1b5) \u8f6c\u819c\u4e0d\u5747\u5300\uff1a\u5370\u8ff9\u819c\u90e8\u5206\u53d8\u5e72\u6216\u6c34\u5316\u4e0d\u5747\u5300\uff1a&nbsp; &nbsp; \u786e\u4fdd\u6574\u5f20\u5370\u8ff9\u819c\u5747\u5300\u6d78\u5165\u8f6c\u79fb\u7f13\u51b2\u6db2\u4e2d\u5e76\u5145\u5206\u5e73\u8861\uff1b&nbsp; &nbsp; PVDF \u819c\u5728\u653e\u5165\u7f13\u51b2\u6db2\u4e4b\u524d\u9700\u8981\u7528 100% \u7684\u7532\u9187\u9884\u5148\u6d78\u6e7f\u5e73\u8861\uff1b\u786c\u4ef6\u95ee\u9898\uff1a&nbsp; &nbsp; \u68c0\u67e5\u6e7f\u8f6c\u88c5\u7f6e\u7684\u7ebf\u8def\u8fde\u63a5\uff1b&nbsp; &nbsp; \u786e\u4fdd\u91d1\u5c5e\u5e73\u677f\u6e05\u6d01\uff0c\u65e0\u7269\u7406\u635f\u4f24\uff0c\u5373\u4fbf\u662f\u91d1\u5c5e\u5e73\u677f\u53d1\u751f\u8f7b\u5fae\u5f2f\u66f2\u4e5f\u53ef\u4ee5\u6781\u5927\u5730\u6539\u53d8\u534a&nbsp; &nbsp; &nbsp; \u5e72\u8f6c\u5370\u7cfb\u7edf\u7684\u7535\u573a\u5f3a\u5ea6\uff1b&nbsp; &nbsp; \u4f7f\u7528\u8d28\u91cf\u53ef\u9760\u7684\u8f6c\u819c\u4eea\uff1b6) \u80cc\u666f\u8fc7\u5f3a\uff1a\u4e00\u6297\u548c\/\u6216\u4e8c\u6297\u6d53\u5ea6\u8fc7\u9ad8\uff1a\u7a00\u91ca\u4e00\u6297\u548c\/\u6216\u4e8c\u6297\u6d53\u5ea6\uff1b\u5c01\u95ed\u4e0d\u8db3\uff1a&nbsp; &nbsp; \u63d0\u9ad8\u5c01\u95ed\u6db2\u6d53\u5ea6\uff0c\u5982 3\u20135% \u7684\u725b\u8840\u6e05\u86cb\u767d\u3001\u916a\u86cb\u767d\u3001 \u8131\u8102\u725b\u5976\uff1b&nbsp; &nbsp; \u964d\u4f4e\u5c01\u95ed\u65f6\u95f4\u548c\/\u6216\u6e29\u5ea6\uff1b&nbsp;&nbsp; &nbsp;\u5411\u5c01\u95ed\u6db2\u4e2d\u52a0\u5165 0.05% \u7684 Tween-20\uff1b&nbsp; &nbsp; \u6297\u4f53\u7a00\u91ca\u6db2\u91c7\u7528\u76f8\u540c\u7684\u5c01\u95ed\u6db2\uff08\u52a0\u5165 0.05%\u7684 Tween-20)\uff1b\u4f7f\u7528\u4e86\u9519\u8bef\u7684\u5c01\u95ed\u6db2\uff0c\u4e00\u6297\u548c\/\u6216\u4e8c\u6297\u4e0e\u5c01\u95ed\u7f13\u51b2\u6db2\u4e2d\u7684\u86cb\u767d\u76f8\u7ed3\u5408\uff1a\u5c1d\u8bd5\u5176\u4ed6\u5c01\u95ed\u6db2\uff08\u767d\u86cb\u767d\u3001\u916a\u86cb\u767d\u3001 \u8131\u8102\u725b\u5976\u7b49\uff09\uff0c\u5728\u4f7f\u7528\u4eb2\u548c\u7d20-\u751f\u7269\u7d20\u7cfb\u7edf\u65f6\u4e0d\u8981\u4f7f\u7528\u725b\u5976\u6765\u5c01\u95ed\u5370\u8ff9\u819c \u2014 \u725b\u5976\u542b\u6709\u751f\u7269\u7d20\uff1b\u6d17\u6da4\u4e0d\u8db3\uff1a&nbsp; &nbsp; \u589e\u52a0\u6d17\u6da4\u6b21\u6570\u548c\u7f13\u51b2\u6db2\u7528\u91cf \uff08\u6700\u5c11 5 \u00d7 5 \u5206\u949f\uff09\uff1b&nbsp; &nbsp; &nbsp;\u5982\u679c\u6d17\u6da4\u6db2\u4e2d\u672a\u542b\u6709 0.1%\u7684 Tween -20\uff0c\u8bf7\u6dfb\u52a0\uff1b\u66dd\u5149\u65f6\u95f4\u8fc7\u957f\uff1a \u51cf\u5c11\u6210\u50cf\u66dd\u5149\u65f6\u95f4\uff1b\u5370\u8ff9\u8fc7\u7a0b\u4e2d\u5370\u8ff9\u819c\u53d8\u5e72\uff1a&nbsp; &nbsp; \u786e\u4fdd\u5370\u8ff9\u819c\u4fdd\u6301\u6e7f\u6da6\uff1b&nbsp; &nbsp; \u786e\u4fdd\u5370\u8ff9\u819c\u4e00\u76f4\u8986\u76d6\u6709\u8db3\u591f\u6db2\u4f53\u4ee5\u9632\u6b62\u5176\u53d8\u5e72\uff1b\u7f13\u51b2\u6db2\u91cd\u590d\u5229\u7528\u5bfc\u81f4\u6c61\u67d3\uff1a\u4f7f\u7528\u65b0\u5236\u7f13\u51b2\u6db2\uff1b7) \u80cc\u666f\u6709\u6c61\u8ff9\uff1a\u90e8\u5206\u5370\u8ff9\u819c\u53d8\u5e72\uff1a&nbsp; &nbsp; \u786e\u4fdd\u5370\u8ff9\u819c\u4fdd\u6301\u6e7f\u6da6\uff1b&nbsp; &nbsp; \u786e\u4fdd\u5370\u8ff9\u819c\u4e00\u76f4\u8986\u76d6\u6709\u8db3\u591f\u6db2\u4f53\u4ee5\u9632\u6b62\u5176\u53d8\u5e72\uff1b\u6d17\u6da4\u7f13\u51b2\u6db2\u4e0e\u67d0\u4e9b\u5370\u8ff9\u533a\u57df\u63a5\u89e6\u4e0d\u8db3\uff1a&nbsp; &nbsp; \u589e\u52a0\u6d17\u6da4\u7f13\u51b2\u6db2\u7528\u91cf\uff1b&nbsp; &nbsp; \u907f\u514d\u8fc7\u591a\u5370\u8ff9\u5806\u53e0\u5728\u4e00\u4e2a\u5b75\u80b2\u5bb9\u5668\u5185\uff1b\u6d17\u6da4\u7f13\u51b2\u6db2\u4e0e\u67d0\u4e9b\u5370\u8ff9\u533a\u57df\u63a5\u89e6\u4e0d\u8db3\uff1a\u4f7f\u7528\u5e72\u51c0\u7684\u6216\u65b0\u7684\u8f6c\u79fb\u201c\u4e09\u660e\u6cbb\u201d\uff1b\u5370\u8ff9\u819c\u6c61\u67d3\uff1a&nbsp; &nbsp; \u8bf7\u52ff\u7528\u624b\u76f4\u63a5\u63a5\u89e6\u5370\u8ff9\u819c\u3002\u4e00\u76f4\u914d\u6234\u6e05\u6d01\u624b\u5957\u6216\u4f7f\u7528\u533b\u7528\u954a\u5b50\uff1b&nbsp; &nbsp; \u786e\u4fdd\u7535\u6cf3\u8bbe\u5907\u3001\u8f6c\u79fb\u8bbe\u5907\u548c\u5b75\u80b2\u76d8\u6e05\u6d01\uff0c\u8fdc\u79bb\u5916\u90e8\u6c61\u67d3\u6e90\uff1b8) \u80cc\u666f\u6709\u6591\u70b9\uff1a\u51dd\u80f6\u788e\u7247\u7c98\u9644\u4e8e\u5370\u8ff9\u819c\u4e4b\u4e0a\uff1a\u5c06\u5370\u8ff9\u819c\u8868\u9762\u6b8b\u7559\u7684\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u788e\u7247\u79fb\u9664\uff1b\u6210\u5757\u7684\u5c01\u95ed\u5242\u7c98\u9644\u4e8e\u5370\u8ff9\u819c\u4e4b\u4e0a\uff1a&nbsp; &nbsp; \u786e\u4fdd\u5c01\u95ed\u5242\u5728\u4f7f\u7528\u524d\u5b8c\u5168\u6eb6\u89e3\uff1b&nbsp; &nbsp; \u5411\u5c01\u95ed\u7f13\u51b2\u6eb6\u6db2\u4e2d\u52a0\u5165 0.1% \u7684 Tween-20\uff1b\u6297\u4f53\u805a\u5408\uff1a&nbsp; &nbsp; \u4f7f\u7528 0.2 \u03bcm \u7684\u8fc7\u6ee4\u5668\u8fc7\u6ee4\u6297\u4f53\u7f13\u51b2\u6db2&nbsp; &nbsp; \u4f7f\u7528\u65b0\u9c9c\u6297\u4f53&nbsp; &nbsp; \u5411\u6297\u4f53\u7f13\u51b2\u6eb6\u6db2\u4e2d\u52a0\u5165 0.1% \u7684 Tween-20\u7f13\u51b2\u6db2\u6c61\u67d3\uff1a&nbsp; &nbsp; \u4f7f\u7528\u65b0\u7684\u7f13\u51b2\u6db2\uff1b&nbsp; &nbsp; \u4f7f\u7528 0.2 \u03bcm \u7684\u8fc7\u6ee4\u5668\u8fc7\u6ee4\u7f13\u51b2\u6db2\uff1b9) \u4e0d\u540c\u6837\u54c1\u95f4\u5185\u53c2\u86cb\u767d\u542b\u91cf\u4e0d\u4e00\u81f4\uff1a\u5728\u5b9e\u9a8c\u8fc7\u7a0b\u4e2d\uff0c\u9009\u4e3a\u5185\u53c2\u7684\u6301\u5bb6\u86cb\u767d\u8868\u8fbe\u6c34\u5e73\u53d1\u751f\u53d8\u5316&nbsp; &nbsp; \u5728\u5b9e\u9a8c\u6761\u4ef6\u4e0b\u8bc6\u522b\u5e76\u9a8c\u8bc1\u9009\u7528\u7684\u5185\u53c2\u86cb\u767d\u7684\u4e00\u81f4\u6027\uff0c\u5373\u8bc1\u5b9e\u76ee\u524d\u5b9e\u9a8c\u6761\u4ef6\u5e76\u4e0d\u4f1a\u6539&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;\u53d8\u8be5\u5185\u53c2\u86cb\u767d\u7684\u8868\u8fbe\u6c34\u5e73\uff1b&nbsp; &nbsp; &nbsp;\u7528\u67d3\u6599\u7ed9\u819c\u4e0a\u7684\u603b\u86cb\u767d\u67d3\u8272\uff0c\u5e76\u4ee5\u819c\u4e0a\u68c0\u6d4b\u5230\u7684\u603b\u86cb\u767d\u4e3a\u5185\u53c2\uff1b10) 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