{"id":45955,"date":"2025-01-05T05:11:03","date_gmt":"2025-01-05T05:11:03","guid":{"rendered":"https:\/\/zydq.1006ss.com\/?p=45955"},"modified":"2025-01-05T05:11:03","modified_gmt":"2025-01-05T05:11:03","slug":"%e4%b8%80%e6%96%87%e8%af%bb%e6%87%82%e7%99%bd%e8%97%9c%e8%8a%a6%e9%86%87%e6%9c%89%e5%93%aa%e4%ba%9b%e6%8a%97%e8%82%bf%e7%98%a4%e4%bd%9c%e7%94%a8%ef%bc%9f","status":"publish","type":"post","link":"http:\/\/zydq.1006ss.com\/?p=45955","title":{"rendered":"\u4e00\u6587\u8bfb\u61c2\u767d\u85dc\u82a6\u9187\u6709\u54ea\u4e9b\u6297\u80bf\u7624\u4f5c\u7528\uff1f"},"content":{"rendered":"

\u767d\u85dc\u82a6\u9187\u662f\u5929\u7136\u7684\u4e8c\u82ef\u4e59\u70ef\u7c7b\u5316\u5408\u7269\uff0c\u5b58\u5728\u4e8e\u8461\u8404\u3001\u77f3\u69b4\u3001\u864e\u6756\u3001\u84dd\u8393\u7b49\u591a\u79cd\u690d\u7269\u4e2d\uff3b1\uff3d\u3002\u7814\u7a76\u8868\u660e\uff0c\u767d\u85dc\u82a6\u9187\u901a\u8fc7\u9776\u5411\u6838\u56e0\u5b50-\u03baB\uff08NF-\u03baB\uff09\u3001\u7ec6\u80de\u6c89\u9ed8\u7ed3\u5408\u86cb\u767d1\uff08Sirt1\uff09\u3001\u7ec6\u80de\u6c89\u9ed8\u7ed3\u5408\u86cb\u767d3\uff08Sirt3\uff09\u3001\u4e73\u9178\u8131\u6c22\u9176\uff08LDH\uff09\u3001\u78f7\u8102\u9170\u808c\u91873-\u6fc0\u9176\uff08PI3K\uff09\u3001\u54fa\u4e73\u52a8\u7269\u96f7\u5e15\u9709\u7d20\u9776\u6807\uff08mTOR\uff09\u3001\u91cd\u7ec4\u4ebaM2\u578b\u4e19\u916e\u9178\u6fc0\u9176\uff08PKM2\uff09\u7b49\u6765\u6291\u5236\u764c\u7ec6\u80de\u751f\u957f\u3001\u4fb5\u88ad\u548c\u589e\u6b96\uff3b2\uff3d\u3002\u540c\u65f6\uff0c\u5b83\u8fd8\u901a\u8fc7\u6fc0\u6d3b\u6291\u764c\u57fa\u56e0p53\u548c\u78f7\u9178\u9176\u5f20\u529b\u86cb\u767d\u540c\u6e90\u7269\uff08PTEN\uff09\u4ee5\u53ca\u4e0b\u8c03PI3K\u7b49\u800c\u4f7f\u7ec6\u80de\u5468\u671f\u505c\u6ede\uff0c\u4ece\u800c\u8bf1\u5bfc\u764c\u7ec6\u80de\u7684\u51cb\u4ea1\uff3b3\uff3d\u3002<\/p>\n

\u6b64\u5916\uff0c\u767d\u85dc\u82a6\u9187\u53ef\u4ee5\u6fc0\u6d3b\u80bf\u7624\u5fae\u73af\u5883\u4e2d\u7684\u514d\u75ab\u7ec6\u80de\uff0c\u4f8b\u5982\u81ea\u7136\u6740\u4f24\uff08NK\uff09\u7ec6\u80de\u3001CD8+\u6dcb\u5df4\u7ec6\u80de\u7b49\uff0c\u589e\u5f3a\u514d\u75ab\u7ec6\u80de\u7684\u6d3b\u6027\uff0c\u5f15\u8d77\u80bf\u7624\u574f\u6b7b\u56e0\u5b50-\u03b1\uff08TNF-\u03b1\uff09\u7684\u91ca\u653e\u4ece\u800c\u8bf1\u5bfc\u764c\u7ec6\u80de\u7684\u51cb\u4ea1\uff3b4\uff3d\u3002\u6b64\u5916\uff0c\u4e0e\u5176\u4ed6\u6297\u764c\u836f\u6bd4\u8f83\uff0c\u767d\u85dc\u82a6\u9187\u4e0d\u4ec5\u53ef\u4ee5\u589e\u5f3a\u764c\u7ec6\u80de\u5bf9\u653e\u5316\u7597\u7684\u654f\u611f\u6027\uff0c\u8fd8\u53ef\u4ee5\u4fdd\u62a4\u6b63\u5e38\u7ec4\u7ec7\u7ec6\u80de\uff3b2\uff3d\u3002<\/p>\n

\u65e2\u5f80\u5bf9\u767d\u85dc\u82a6\u9187\u6297\u80bf\u7624\u4f5c\u7528\u7684\u7814\u7a76\u4ec5\u4ece\u67d0\u79cd\u6216\u51e0\u79cd\u673a\u5236\u5c55\u5f00\u7efc\u8ff0\uff0c\u672a\u8fdb\u884c\u8f83\u7cfb\u7edf\u5b8c\u5584\u7684\u9610\u8ff0\u3002\u672c\u6587\u4ece\u767d\u85dc\u82a6\u9187\u5728\u4e0d\u540c\u5b9e\u4f53\u7624\u4e2d\u7684\u6297\u80bf\u7624\u4f5c\u7528\u673a\u5236\u5c55\u5f00\u7efc\u8ff0\uff0c\u66f4\u80fd\u4f53\u73b0\u767d\u85dc\u82a6\u9187\u6297\u80bf\u7624\u4f5c\u7528\u7684\u5e7f\u6cdb\u6027\uff0c\u4ee5\u671f\u4e3a\u52a0\u5feb\u4e34\u5e8a\u6297\u80bf\u7624\u65b0\u836f\u7684\u7814\u53d1\u63d0\u4f9b\u66f4\u5145\u8db3\u7684\u8bc1\u636e\u652f\u6301\uff0c\u4ee5\u4fc3\u8fdb\u767d\u85dc\u82a6\u9187\u4e34\u5e8a\u5236\u5242\u7684\u751f\u4ea7\u4f7f\u7528\uff0c\u4f7f\u66f4\u591a\u764c\u75c7\u60a3\u8005\u53d7\u76ca\u3002<\/p>\n

1\u3000\u7ed3\u80a0\u764c<\/h2>\n

1.1\u3000\u6291\u5236\u80bf\u7624\u8840\u7ba1\u751f\u6210<\/h2>\n

\u4e54\u7490\u7b49\uff3b5\uff3d\u7814\u7a76\u4e86\u5bf9\u7167\u7ec4\u53ca\u767d\u85dc\u82a6\u9187\u4f4e\u3001\u4e2d\u548c\u9ad8\u5242\u91cf\u7ec4\uff080\u300125\u300150\u3001100 \u00b5mol\/L\uff09\u5904\u740624 h\u540e\u7684\u4f53\u5916\u6a21\u578b\u7ed3\u80a0\u764c\u7ec6\u80de\u7cfb\u3002\u7ed3\u679c\u663e\u793a\uff0c\u4e2d\u3001\u9ad8\u5242\u91cf\uff0850\u3001100 \u00b5mol\/L\uff09\u767d\u85dc\u82a6\u9187\u5904\u7406\u7ed3\u80a0\u764c\u7ec6\u80deHT-29\u3001sw480\u548cLoVo\u7ec6\u80de\u540e\uff0c\u764c\u7ec6\u80de\u51cb\u4ea1\u7387\u663e\u8457\u4e0a\u5347\uff0c\u5e76\u5448\u5242\u91cf\u76f8\u5173\uff0c\u4e14\u7ec6\u80de\u4e2d\u9ad3\u6837\u5206\u5316\u56e0\u5b50\uff08MyD88\uff09\u3001Toll\u6837\u53d7\u4f53-4\uff08TLR-4\uff09\u548cNF-\u03baB\u542b\u91cf\u5747\u660e\u663e\u964d\u4f4e\u3002\u7814\u7a76\u8fd8\u53d1\u73b0\uff0c\u767d\u85dc\u82a6\u9187\u5904\u7406\u540e\u7ed3\u80a0\u764c\u7ec6\u80de\u4e0a\u6e05\u6db2\u4e2d\u8840\u7ba1\u5185\u76ae\u751f\u957f\u56e0\u5b50\uff08VEGF\uff09\u86cb\u767d\u8868\u8fbe\u660e\u663e\u964d\u4f4e\uff0c\u63d0\u793a\u767d\u85dc\u82a6\u9187\u53ef\u80fd\u901a\u8fc7\u5f71\u54cd\u7ed3\u80a0\u764c\u8840\u7ba1\u751f\u6210\u800c\u8bf1\u5bfc\u764c\u7ec6\u80de\u51cb\u4ea1\u3002<\/p>\n

1.2\u3000\u5e72\u6270\u76f8\u5173\u4fe1\u53f7\u8f6c\u5bfc\u901a\u8def<\/h2>\n

\u7814\u7a76\u53d1\u73b0\uff0c\u767d\u85dc\u82a6\u9187\u53ef\u901a\u8fc7NF-\u03baB\u3001Sirt1\u3001Sirt3\u3001LDH\u3001PI3K\u3001mTOR\u7b49\u591a\u79cd\u4fe1\u53f7\u901a\u8def\u6291\u5236\u7ed3\u80a0\u764c\u7ec6\u80de\u7684\u589e\u6b96\u3001\u4fb5\u88ad\u548c\u4fc3\u8fdb\u5176\u51cb\u4ea1\uff3b6\uff3d\u3002\u8096\u949f\u80dc\u7b49\uff3b7\uff3d\u4f7f\u75281\u7ec4\u4f53\u5916\u57f9\u517b\u7684\u4eba\u7ed3\u76f4\u80a0\u764cLoVo\u7ec6\u80de\uff0c\u91c7\u7528\u567b\u5511\u84dd\uff08MTT\uff09\u6bd4\u8272\u6cd5\u5206\u6790\u4e0d\u540c\u6d53\u5ea6\u7684\u767d\u85dc\u82a6\u91870.0125\u30010.025\u30010.05\u30010.1\u30010.2 mmol\/L\u5bf9LoVo\u7ec6\u80de\u589e\u6b96\u80fd\u529b\u7684\u5f71\u54cd\u3002\u7ed3\u679c\u8868\u660e\uff0c\u968f\u7740\u767d\u85dc\u82a6\u9187\u6d53\u5ea6\u7684\u589e\u52a0\uff0c\u5176\u5bf9LoVo\u7ec6\u80de\u589e\u6b96\u6291\u5236\u7387\u5747\u9010\u6e10\u4e0a\u5347\uff0c\u5e76\u5177\u6709\u65f6\u95f4\u548c\u5242\u91cf\u76f8\u5173\u6027\uff0c\u4e14\u4e0e\u5bf9\u7167\u7ec4\u6bd4\u8f83\uff0c\u767d\u85dc\u82a6\u9187\u53ef\u663e\u8457\u51cf\u5c11\u7ec6\u80de\u5bf9\u8461\u8404\u7cd6\u7684\u6444\u53d6\u80fd\u529b\uff0c\u540c\u65f6\u53ef\u663e\u8457\u964d\u4f4e\u7ec6\u80de\u5185\u78f7\u9178\u5316\u54fa\u4e73\u52a8\u7269\u96f7\u5e15\u9709\u7d20\u9776\u6807\uff08p-mTOR\uff09\u6c34\u5e73\u3002<\/p>\n

Brockmueller\u7b49\uff3b8\uff3d\u7814\u7a76\u6587\u732e\u65f6\u53d1\u73b0\uff0c\u5728\u7ed3\u80a0\u764c\u7ec6\u80de\u4e2d\uff0c\u767d\u85dc\u82a6\u9187\u901a\u8fc7\u9499\u8c03\u86cb\u767d\/\u9499\u8c03\u7d20\u4f9d\u8d56\u86cb\u767d\u6fc0\u9176\uff08CamKKB\uff09\/\u817a\u82f7\u9178\u6fc0\u6d3b\u86cb\u767d\u6fc0\u9176\uff08AMPK\uff09\u4fe1\u53f7\u901a\u8def\u63d0\u9ad8\u7ed3\u80a0\u764c\u7ec6\u80de\u7684\u6c27\u5316\u80fd\u529b\uff0c\u5e76\u901a\u8fc7\u9776\u5411\u6d3b\u6027\u6c27\uff08ROS\uff09\u4ecb\u5bfc\u7684\u7f3a\u6c27\u8bf1\u5bfc\u56e0\u5b501\u03b1\u591a\u80bd\uff08HIF-1\u03b1\uff09\u6fc0\u6d3b\u6765\u6291\u5236\u8461\u8404\u7cd6\u6444\u53d6\uff0c\u5728\u4f53\u5916\u548c\u4f53\u5185\u6291\u5236\u8461\u8404\u7cd6\u4ee3\u8c22\u548c\u80bf\u7624\u751f\u957f\uff0c\u5e76\u901a\u8fc7\u9776\u5411\u78f7\u9178\u620a\u7cd6\u548c\u8e1d\u86cb\u767d\/\u9ecf\u7740\u6591\u6fc0\u9176\uff08talin-FAK\uff09\u4fe1\u53f7\u901a\u8def\u8bf1\u5bfc\u764c\u7ec6\u80de\u51cb\u4ea1\u3002<\/p>\n

Buhrmann\u7b49\uff3b9\uff3d\u7528\u591a\u7ec6\u80de-\u80bf\u7624\u5fae\u73af\u5883\uff08TME\uff09\u57f9\u517b\u7269\uff08\u6210\u7ea4\u7ef4\u7ec6\u80de\u3001T\u6dcb\u5df4\u7ec6\u80de\uff09\u57f9\u517b\u4f53\u5916\u6a21\u578b\u7ed3\u80a0\u764c\u7ec6\u80de\u7cfbHCT116\u7ec6\u80de\u7528\u4e0d\u540c\u6d53\u5ea6\u7684\u767d\u85dc\u82a6\u9187\u5904\u740614 d\uff0c\u5e76\u7528MTT\u6cd5\u8fdb\u884c\u6d4b\u5b9a\u3002\u7ed3\u679c\u53d1\u73b0\uff0c\u4e0e\u57fa\u7840\u5bf9\u7167\u7ec4\u6bd4\u8f83\uff0c\u7ecf\u767d\u85dc\u82a6\u9187\u5904\u7406\u540e\u7684HCT116\u7ec6\u80de\u4ee5\u5242\u91cf\uff081\u30012\u30015\u300110 \u00b5mol\/L\uff09\u76f8\u5173\u7684\u65b9\u5f0f\u963b\u65ad\u4e86\u764c\u7ec6\u80de\u7684\u7ec6\u80de\u6d3b\u529b\u548c\u589e\u6b96\u80fd\u529b\uff0827%\u300138%\u300166%\u548c86%\uff09\u3002\u6b64\u5916\uff0c\u4f53\u5916\u591a\u7ec6\u80de-TME\u53ef\u4ee5\u4fc3\u8fdb\u7ed3\u80a0\u764c\u7ec6\u80de\u548c\u57fa\u8d28\u7ec6\u80de\u4e4b\u95f4\u7684\u4e32\u6270\uff0c\u8bf1\u5bfcHCT116\u7ec6\u80de\u7684\u751f\u957f\u548c\u8f6c\u79fb\uff0c\u800c\u767d\u85dc\u82a6\u9187\u901a\u8fc7\u4e0eSirt1\u86cb\u767d\u7ed3\u5408\u6210\u767d\u85dc\u82a6\u9187-Sirt1\u8f74\uff0c\u8be5\u8f74\u901a\u8fc7\u8c03\u8282\u65c1\u5206\u6ccc\u7269\u548cNF-\u03baB\u4fe1\u53f7\u901a\u8def\u6765\u6291\u5236\u7ed3\u80a0\u764c\u7ec6\u80de\u7684\u751f\u957f\u548c\u8f6c\u79fb\uff0c\u8fd9\u8868\u660e\u767d\u85dc\u82a6\u9187\u53ef\u53c2\u4e0e\u963b\u6b62HCT116\u7ec6\u80de\u7684\u751f\u957f\u53ca\u8f6c\u79fb\u7684\u8fc7\u7a0b\u3002<\/p>\n

2\u3000\u80f0\u817a\u764c<\/h2>\n

2.1\u3000\u8c03\u63a7\u76f8\u5173\u86cb\u767d\u8868\u8fbe<\/h2>\n

Ratajczak\u7b49\uff3b10\uff3d\u7528\u4e0d\u540c\u6d53\u5ea6\uff080\u30015\u300110\u300125\u300150\u3001100\u3001150\u3001200 \u00b5mol\/L\uff09\u767d\u85dc\u82a6\u9187\u5904\u7406\u80f0\u817a\u764c\u7ec6\u80de24\u300148\u300172 h\uff0cMTT\u6cd5\u5206\u6790\u7ed3\u679c\u663e\u793a\uff0c\u4e0e\u5bf9\u7167\u7ec4\u6bd4\u8f83\uff0c\u767d\u85dc\u82a6\u9187\u5904\u7406\u540e\u7684\u80f0\u817a\u764c\u7ec6\u80de\u589e\u6b96\u6291\u5236\u7387\u660e\u663e\u5347\u9ad8\uff0c\u5177\u6709\u65f6\u95f4\u548c\u6d53\u5ea6\u76f8\u5173\u6027\u3002\u540c\u65f6\u4ed6\u4eec\u91c7\u7528\u6d41\u5f0f\u7ec6\u80de\u4eea\u5206\u6790\u4e0d\u540c\u6d53\u5ea6\uff080\u300125\u300150\u3001100 \u00b5mol\/L\uff09\u767d\u85dc\u82a6\u9187\u5904\u7406\u80f0\u817a\u764c\u7ec6\u80de48 h\uff0c\u7ed3\u679c\u8868\u660e\uff0c\u767d\u85dc\u82a6\u9187\u4ee5\u6d53\u5ea6\u76f8\u5173\u6027\u65b9\u5f0f\u8bf1\u5bfc\u764c\u7ec6\u80de\u51cb\u4ea1\u3002\u7814\u7a76\u8fd8\u53d1\u73b0\u767d\u85dc\u82a6\u9187\u901a\u8fc7\u6539\u53d8\u4e0e\u51cb\u4ea1\u8fc7\u7a0b\u76f8\u5173\u86cb\u767d\u8d28\u7684\u8868\u8fbe\uff08\u4fc3\u51cb\u4ea1\u86cb\u767dBax\u6c34\u5e73\u5347\u9ad8\u548c\u6297\u51cb\u4ea1\u86cb\u767dBcl-2\u6c34\u5e73\u964d\u4f4e\uff09\u800c\u53d1\u6325\u5bf9\u80f0\u817a\u764c\u7ec6\u80de\u7684\u6297\u589e\u6b96\u548c\u4fc3\u51cb\u4ea1\u7684\u4f5c\u7528\u3002<\/p>\n

Jiang\u7b49\uff3b11\uff3d\u4f7f\u7528200 \u00b5mol\/L\u767d\u85dc\u82a6\u9187\u5904\u7406\u4eba\u80f0\u817a\u764c\u7ec6\u80de\u7cfbPANC-1\u548cSW1990\u7ec6\u80de\u5206\u522b12\u300124\u300136\u300148 h\uff0c\u7ed3\u679c\u8868\u660e\u767d\u85dc\u82a6\u9187\u53ef\u4ee5\u5728\u4f53\u5916\u6291\u5236\u80f0\u817a\u764c\u7ec6\u80de\u589e\u6b96\uff0c\u4ed6\u4eec\u8fd8\u901a\u8fc7\u5212\u75d5\u5b9e\u9a8c\u548c\u7ec6\u80de\u8fc1\u79fb\u4fb5\u88ad\uff08transwell\uff09\u8bd5\u9a8c\u9a8c\u8bc1\u4e86\u767d\u85dc\u82a6\u9187\u53ef\u4ee5\u6291\u5236\u80f0\u817a\u764c\u7ec6\u80de\u7684\u589e\u6b96\u3001\u8fc1\u79fb\u548c\u4fb5\u88ad\u3002\u540c\u65f6\uff0c\u5b9e\u9a8c\u7ed3\u679c\u8868\u660e\uff0c\u767d\u85dc\u82a6\u9187\u901a\u8fc7\u4e0a\u8c03\u80f0\u817a\u764c\u7ec6\u80de\u4e2dPTEN\u7684\u8868\u8fbe\u6c34\u5e73\u6765\u6291\u5236\u4fc3\u764c\u53d7\u4f53\u86cb\u767d2\uff08RYR2\uff09\u7684\u8868\u8fbe\uff0c\u4ece\u800c\u6291\u5236\u80f0\u817a\u764c\u7684\u53d1\u5c55\u3002<\/p>\n

2.2\u3000\u6291\u5236\u80bf\u7624\u53d1\u5c55<\/h2>\n

Qian\u7b49\uff3b12\uff3d\u4f7f\u7528\u767d\u85dc\u82a6\u918750 mg\/(kg\u00b7d)\u5206\u522b\u6cbb\u7597\u80f0\u817a\u764c\u5c0f\u9f20\u6a21\u578b1\u30013\u30016\u4e2a\u6708\uff0c\u7ed3\u679c\u4e0e\u57fa\u7840\u5bf9\u7167\u7ec4\u6bd4\u8f83\uff0c\u767d\u85dc\u82a6\u9187\u5728\u75be\u75c5\u8fdb\u5c55\u9636\u6bb5\uff083\u62166\u4e2a\u6708\uff09\u901a\u8fc7\u51cf\u8f7b\u80f0\u817a\u764c\u524d\u75c5\u53d8\u7684\u4e25\u91cd\u7a0b\u5ea6\u6765\u5ef6\u7f13\u80bf\u7624\u7684\u53d1\u5c55\uff0c\u4f46\u8fd9\u79cd\u5ef6\u7f13\u662f\u901a\u8fc7\u51cf\u7f13\u5176\u4ece\u4f4e\u7ea7\u522b\u75c5\u53d8\u5411\u9ad8\u7ea7\u522b\u75c5\u53d8\u7684\u8fdb\u5c55\u4f46\u4e0d\u80fd\u963b\u6b62\u6076\u6027\u75c5\u53d8\uff0c\u8fd9\u4e00\u8fc7\u7a0b\u53ef\u80fd\u4e0e\u767d\u85dc\u82a6\u9187\u6291\u5236NF-\u03baB\u6d3b\u5316\u6709\u5173\u3002\u4ed6\u4eec\u8fd8\u53d1\u73b0\uff0c\u767d\u85dc\u82a6\u9187\u4e0d\u4ec5\u6291\u5236NF-\u03baB\u6d3b\u5316\uff0c\u8fd8\u80fd\u6291\u5236\u7ec6\u80de\u5916\u8c03\u8282\u86cb\u767d\u6fc0\u9176\uff08ERK\uff09\/\u4fe1\u53f7\u4f20\u5bfc\u4e0e\u8f6c\u5f55\u6fc0\u6d3b\u56e0\u5b50\uff08STAT3\uff09\u4fe1\u53f7\u901a\u8def\u6fc0\u6d3b\u800c\u53d1\u6325\u5316\u7597\u589e\u654f\u7684\u6548\u679c\u3002<\/p>\n

3\u3000\u5375\u5de2\u764c<\/h2>\n

3.1\u3000\u5e72\u6270\u76f8\u5173\u4fe1\u53f7\u8f6c\u5bfc\u901a\u8def<\/h2>\n

\u9a6c\u667a\u7b49\uff3b13\uff3d\u7528\u4e0d\u540c\u6d53\u5ea6\uff0850\u3001100\u3001200\u3001400 \u00b5mol\/L\uff09\u767d\u85dc\u82a6\u9187\u4f5c\u7528\u4e8e\u4eba\u5375\u5de2\u817a\u764cA2780\u7ec6\u80de\u4e0d\u540c\u65f6\u95f4\uff0824\u300148\u300172 h\uff09\uff0c\u7ed3\u679c\u5f97\u51fa\uff0c200 \u00b5mol\/L\u7684\u767d\u85dc\u82a6\u9187\u4f5c\u752824 h\u53ef\u6709\u6548\u6291\u5236A2780\u7ec6\u80de\u589e\u6b96\uff0c\u4f7f\u5176\u963b\u6ede\u4e8eG0\/G1\u671f\uff0c\u5177\u6709\u663e\u8457\u7684\u5242\u91cf-\u65f6\u95f4\u76f8\u5173\u6027\uff0c\u5176\u53ef\u80fd\u7684\u673a\u5236\u4e0e\u767d\u85dc\u82a6\u9187\u901a\u8fc7\u4e0b\u8c03\u6574\u5408\u7d20\u8fde\u63a5\u6fc0\u9176\uff08ILK\uff09\/\u03b2-\u73af\u8fde\u86cb\u767d\uff08\u03b2-catenin\uff09\u7684\u8868\u8fbe\uff0c\u4f7fWnt\/\u03b2-catenin\u4fe1\u53f7\u901a\u8def\u6d3b\u6027\u964d\u4f4e\uff0c\u4e0b\u6e38\u9776\u57fa\u56e0\u7ec6\u80de\u5468\u671f\u86cb\u767d\uff08Cyclin\uff09D1\u7684\u8868\u8fbe\u53d7\u5230\u6291\u5236\u6709\u5173\u3002<\/p>\n

\u738b\u4e3d\u5a1f\u7b49\uff3b14\uff3d\u7684\u8bd5\u9a8c\u4e5f\u5f97\u51fa\u76f8\u540c\u7684\u7ed3\u8bba\uff0c\u5e76\u4e14\u53d1\u73b0\u767d\u85dc\u82a6\u9187\u53ef\u901a\u8fc7\u8c03\u63a7Sirt1\u7684mRNA\u548c\u86cb\u767d\u8868\u8fbe\u6c34\u5e73\u6291\u5236\u5375\u5de2\u764c\u7ec6\u80de\u589e\u6b96\uff0c\u4fc3\u8fdb\u51cb\u4ea1\uff0c\u5176\u4fc3\u51cb\u4ea1\u673a\u5236\u53ef\u80fd\u4e0eWnt\u4fe1\u53f7\u901a\u8def\u5931\u6d3b\u6709\u5173\u3002<\/p>\n

\u674e\u677e\u5ca9\u7b49\uff3b15\uff3d\u4f7f\u7528\u4e0d\u540c\u6d53\u5ea6\uff082.5\u30015\u300110\u300120\u300140\u300180 mg\/L\uff09\u767d\u85dc\u82a6\u9187\u4f5c\u7528\u4eba\u5375\u5de2\u764cSKOV3\u7ec6\u80de24 h\u540e\u8fdb\u884c\u6307\u6807\u68c0\u6d4b\u3002\u7ed3\u679c\u663e\u793a\uff0c\u767d\u85dc\u82a6\u9187\u4f5c\u7528\u540e\u7684SKOV3\u7ec6\u80de\u6d3b\u529b\u663e\u8457\u964d\u4f4e\u3002\u5e76\u4e14\u4e0e\u5bf9\u7167\u7ec4\u6bd4\u8f83\uff0c\u767d\u85dc\u82a6\u9187\u5404\u6d53\u5ea6\u7ec4\u4e2dROS\u7ea2\u8272\u8367\u5149\u6570\u91cf\u663e\u8457\u51cf\u5c11\uff0cSirt3\u3001\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff092\u3001\u6297\u4eba\u5355\u514b\u9686\u6297\u4f53\uff08Bax\uff09\u548c\u88c2\u89e3\u7684\u5929\u51ac\u6c28\u9178\u7279\u5f02\u6027\u534a\u80f1\u6c28\u9178\u86cb\u767d\u91763\uff08cleaved caspase-3\uff09\u86cb\u767d\u8868\u8fbe\u663e\u8457\u4e0a\u5347\u800cB\u6dcb\u5df4\u7ec6\u80de\u7624-2\uff08Bcl-2\uff09\u86cb\u767d\u8868\u8fbe\u663e\u8457\u964d\u4f4e\u3002\u4ee5\u4e0a\u7ed3\u679c\u663e\u793a\u767d\u85dc\u82a6\u9187\u53ef\u4ee5\u901a\u8fc7\u8c03\u63a7Sirt3-SOD2-ROS\u4fe1\u53f7\u901a\u8def\u8bf1\u5bfc\u4eba\u5375\u5de2\u764cSKOV3\u7ec6\u80de\u51cb\u4ea1\u3002<\/p>\n

3.2\u3000\u9006\u8f6c\u5316\u7597\u8010\u836f\u53ca\u589e\u5f3a\u5316\u7597\u654f\u611f\u6027<\/h2>\n

\u738b\u6167\u7b49\uff3b16\uff3d\u63a2\u8ba8\u4e86\u767d\u85dc\u82a6\u9187\u5bf9\u4f53\u5916\u57f9\u517b\u4eba\u5375\u5de2\u764c\u987a\u94c2\u8010\u836f\uff08SKOV3\/DDP\uff09\u7ec6\u80de\u589e\u654f\u7684\u4f5c\u7528\u53ca\u673a\u5236\u3002\u8bd5\u9a8c\u5206\u4e3a3\u7ec4\uff1a\u5bf9\u7167\u7ec4\u3001\u987a\u94c2\u7ec4\uff0832 \u03bcg\/mL\uff09\u548c\u767d\u85dc\u82a6\u9187\uff0b\u987a\u94c2\u7ec4\uff08\u767d\u85dc\u82a6\u918720 \u03bcg\/mL\uff0b\u987a\u94c232 \u03bcg\/mL\uff09\uff0c\u5747\u5e72\u988424 h\u3002\u7ed3\u679c\u5f97\u51fa\uff0c\u767d\u85dc\u82a6\u9187\uff0b\u987a\u94c2\u7ec4\u548c\u987a\u94c2\u7ec4\u7684\u7ec6\u80de\u6291\u5236\u7387\u3001\u7ec6\u80de\u51cb\u4ea1\u7387\u3001Bax\u53ca\u6291\u764c\u57fa\u56e0p21\u8868\u8fbe\u6c34\u5e73\u5747\u9ad8\u4e8e\u5bf9\u7167\u7ec4\uff0c\u4e14\u767d\u85dc\u82a6\u9187\uff0b\u987a\u94c2\u7ec4\u5747\u9ad8\u4e8e\u987a\u94c2\u7ec4\u3002\u8be5\u7814\u7a76\u8868\u660e\u767d\u85dc\u82a6\u9187\u53ef\u589e\u52a0SKOV3\/DDP\u7ec6\u80de\u5bf9\u987a\u94c2\u5316\u7597\u654f\u611f\u6027\uff0c\u4fc3\u8fdbSKOV3\/DDP\u7ec6\u80de\u5185Bax\u548cp21\u7684\u8868\u8fbe\uff0c\u5e76\u4fc3\u8fdb\u7ec6\u80de\u51cb\u4ea1\u3002<\/p>\n

\u738b\u6167\u7b49\uff3b17\uff3d\u53e61\u9879\u7814\u7a76\u53d1\u73b0\uff0c\u767d\u85dc\u82a6\u9187\u53ef\u6709\u6548\u9006\u8f6c\u4eba\u5375\u5de2\u764cSKOV3\/DDP\u7ec6\u80de\u7684\u987a\u94c2\u5316\u7597\u8010\u836f\u6027\uff0c\u5176\u673a\u5236\u53ef\u80fd\u4e0e\u5176\u53ef\u901a\u8fc7\u4e0b\u8c03\u591a\u836f\u8010\u836f\u57fa\u56e01\uff08MDR-1\uff09\u548cBcl-2\u7684mRNA\u8868\u8fbe\u4ee5\u51cf\u5c11P-\u7cd6\u86cb\u767d\uff08P-gp\uff09\u86cb\u767d\u8868\u8fbe\u53ca\u836f\u7269\u5916\u6392\uff0c\u7ef4\u6301\u7ec6\u80de\u5185\u7684DDP\u6d53\u5ea6\u6709\u5173\u3002<\/p>\n

4\u3000\u4e73\u817a\u764c<\/h2>\n

4.1\u3000\u6291\u5236\u764c\u7ec6\u80de\u589e\u6b96\u53ca\u589e\u5f3a\u5316\u7597\u654f\u611f\u6027<\/h2>\n

Cheuk\u7b49\uff3b18\uff3d\u7814\u7a76\u4f53\u5916\u57f9\u517bMDA-MB-231\u3001\u987a\u94c2\u8010\u836f\u682aMDA-MB-231\u548cT47D\u4e73\u817a\u764c\u7ec6\u80de\uff0c\u7528\u542b\u6216\u4e0d\u542b\u767d\u85dc\u82a6\u918725 \u00b5mol\/L\u57f9\u517b3 d\u540e\u4e0e\u5355\u7eaf\u4f7f\u7528\u987a\u94c2\u6216\u767d\u85dc\u82a6\u9187\u6bd4\u8f83\uff0c\u4e8c\u8005\u8054\u5408\u4f7f\u7528\u7ec6\u80de\u589e\u6b96\u6291\u5236\u7387\u66f4\u9ad8\u3002\u767d\u85dc\u82a6\u9187\u8fd8\u53ef\u589e\u5f3aM1\u5de8\u566c\u7ec6\u80de\u6781\u5316\u4f7fTME\u4e2d\u4fc3\u708e\u7ec6\u80de\u56e0\u5b50\u767d\u7ec6\u80de\u4ecb\u7d20-6\uff08IL-6\uff09\u6c34\u5e73\u663e\u8457\u964d\u4f4e\uff0c\u964d\u4f4e\u5316\u7597\u8010\u836f\u6027\uff0c\u4f7f\u4e73\u817a\u764c\u7ec6\u80de\u5bf9\u987a\u94c2\u66f4\u654f\u611f\u3002\u6b64\u5916\uff0c\u4ed6\u4eec\u8fd8\u5728\u975e\u80a5\u80d6\u7cd6\u5c3f\u75c5\/\u91cd\u75c7\u8054\u5408\u514d\u75ab\u7f3a\u9677\uff08NOD\/SCID\uff09\u5c0f\u9f20\u7684\u5f02\u79cd\u79fb\u690d\u80bf\u7624\u4e2d\u7814\u7a76\u4e86Ki-67\u3001STAT3\u548c\u78f7\u9178\u5316\u4fe1\u53f7\u4f20\u5bfc\u4e0e\u8f6c\u5f55\u6fc0\u6d3b\u56e0\u5b50\uff08p-STAT3\uff09\u7684\u8868\u8fbe\u60c5\u51b5\uff0c\u514d\u75ab\u7ec4\u5316\u67d3\u8272\u7ed3\u679c\u663e\u793a\uff0c\u4e8c\u8005\u8054\u5408\u6cbb\u7597\u53ef\u901a\u8fc7\u964d\u4f4ep-STAT3\u7684\u6c34\u5e73\u6765\u6291\u5236\u80bf\u7624\u751f\u957f\u3002\u603b\u4e4b\uff0c\u767d\u85dc\u82a6\u9187\u53ef\u9006\u8f6c\u4e73\u817a\u764c\u4e2d\u5de8\u566c\u7ec6\u80de\u6781\u5316\u3001\u964d\u4f4eIL-6\u6c34\u5e73\u548c\u6291\u5236STAT3\u7684\u6d3b\u5316\u800c\u663e\u8457\u964d\u4f4e\u4e73\u817a\u764c\u7ec6\u80de\u7684\u589e\u6b96\u548c\u589e\u5f3a\u5bf9\u5316\u7597\u7684\u654f\u611f\u6027\u3002<\/p>\n

4.2\u3000\u8c03\u63a7\u76f8\u5173\u86cb\u767d\u8868\u8fbe<\/h2>\n

\u738b\u68a6\u6b23\u7b49\uff3b19\uff3d\u7814\u7a76\u4e86\u4e0d\u540c\u6d53\u5ea6\uff080\u30011\u30012.5\u30015\u300110\u300125\u300150 \u00b5mol\/L\uff09\u767d\u85dc\u82a6\u9187\u5bf9\u4f53\u5916\u57f9\u517b\u4eba\u4e73\u817a\u764cMDA-MB-231\u7ec6\u80de\u6d3b\u6027\u7684\u6291\u5236\u80fd\u529b\u3002\u7ed3\u679c\u663e\u793a\uff0c\u767d\u85dc\u82a6\u9187\u5bf9MDA-MB-231\u7ec6\u80de\u5177\u6709\u663e\u8457\u7684\u6740\u4f24\u80fd\u529b\uff0c\u4e14\u7ec6\u80de\u7684\u5b58\u6d3b\u7387\u968f\u7740\u767d\u85dc\u82a6\u9187\u523a\u6fc0\u6d53\u5ea6\u589e\u52a0\u800c\u964d\u4f4e\uff0c\u572825 \u00b5mol\/L\u51fa\u73b0\u660e\u663e\u7684\u6740\u4f24\u764c\u7ec6\u80de\u7684\u4f5c\u7528\uff0c\u5177\u6709\u660e\u663e\u7684\u6d53\u5ea6\u76f8\u5173\u6027\u3002\u7ed3\u679c\u8868\u660e\uff0c\u767d\u85dc\u82a6\u9187\u5904\u7406\u540eMDA-MB-231\u7ec6\u80de\u7684\u8fc1\u79fb\u548c\u4fb5\u88ad\u80fd\u529b\u660e\u663e\u5f97\u5230\u6291\u5236\u3002\u86cb\u767d\u5370\u8ff9\uff08Western blotting\uff09\u6cd5\u68c0\u6d4b\u7ed3\u679c\u663e\u793a\uff0c\u767d\u85dc\u82a6\u9187\u80fd\u591f\u964d\u4f4e\u4ebaDNA\u805a\u5408\u9176\u03b41\uff08POLD1\uff09\u3001N-cadherin\u53ca\u6ce2\u5f62\u7ea4\u7ef4\u86cb\u767d\uff08Vimentin\uff09\u7684\u8868\u8fbe\uff0c\u4e0a\u8c03E-\u9499\u9ecf\u86cb\u767d\uff08E-cadherin\uff09\u7684\u8868\u8fbe\u3002\u901a\u8fc7\u4ee5\u4e0a\u7ed3\u679c\u5f97\u51fa\uff0c\u767d\u85dc\u82a6\u9187\u80fd\u591f\u6291\u5236MDA-MB-231\u7ec6\u80de\u7684\u6d3b\u6027\u53ca\u4e0a\u76ae-\u95f4\u8d28\u8f6c\u5316\uff08EMT\uff09\uff0c\u4e0b\u8c03POLD1\u662f\u5176\u5173\u952e\u673a\u5236\u4e4b\u4e00\u3002<\/p>\n

5\u3000\u80ba\u764c<\/h2>\n

5.1\u3000\u6291\u5236\u764c\u7ec6\u80de\u589e\u6b96\u53ca\u8bf1\u5bfc\u5176\u51cb\u4ea1<\/h2>\n

Li\u7b49\uff3b20\uff3d\u4ee5\u4eba\u5c0f\u7ec6\u80de\u80ba\u764cH446\u7ec6\u80de\u4e3a\u7814\u7a76\u5bf9\u8c61\uff0c\u7528\u4e0d\u540c\u6d53\u5ea6\u7684\u767d\u85dc\u82a6\u9187\u5904\u7406\u4f5c\u7528\u4e0d\u540c\u65f6\u95f4\u3002MTT\u6cd5\u68c0\u6d4b\u53d1\u73b0\uff0c\u767d\u85dc\u82a6\u9187\u80fd\u591f\u660e\u663e\u6291\u5236H446\u7ec6\u80de\u7684\u589e\u6b96\uff0c30 \u00b5g\u00b7mL\uff0d1\u6291\u5236\u7387\u5f00\u59cb\u4e0a\u5347\uff0c40 \u00b5g\u00b7mL-1\u65f6\u6291\u5236\u7387\u8fc5\u901f\u4e0a\u5347\uff0c\u66f4\u9ad8\u6d53\u5ea6\u65f6\u53ef\u5f15\u8d77\u7ec6\u80de\u6b7b\u4ea1\uff0c\u4f5c\u7528\u65f6\u95f4\u4e0a24 h\u65f6\u4ea7\u751f\u660e\u663e\u6291\u5236\uff0c48 h\u65f6\u6291\u5236\u7387\u63a5\u8fd170%\uff0c\u5177\u6709\u6d53\u5ea6\u548c\u65f6\u95f4\u76f8\u5173\u6027\u3002<\/p>\n

\u6d41\u5f0f\u7ec6\u80de\u672f\u68c0\u6d4b\u7ed3\u679c\u663e\u793a\uff0c\u767d\u85dc\u82a6\u9187\u53ef\u8bf1\u5bfcH446\u7ec6\u80de\u51cb\u4ea1\u3001\u4fc3\u8fdb\u7ec6\u80de\u5185ROS\u7684\u91ca\u653e\u3001\u4f7f\u7ebf\u7c92\u4f53\u819c\u7535\u4f4d\u4e0b\u964d\uff0c\u4e14\u968f\u7740\u5242\u91cf\u7684\u589e\u52a0\u548c\u65f6\u95f4\u7684\u5ef6\u957f\uff0c\u7ec6\u80de\u51cb\u4ea1\u7684\u4f5c\u7528\u66f4\u5f3a\u3001ROS\u91ca\u653e\u66f4\u591a\u3001\u7ebf\u7c92\u4f53\u819c\u7535\u4f4d\u4e0b\u964d\u66f4\u5feb\uff0c\u5448\u660e\u663e\u7684\u5242\u91cf\u548c\u65f6\u95f4\u76f8\u5173\u3002\u8be5\u7814\u7a76\u8868\u660e\uff0c\u767d\u85dc\u82a6\u9187\u53ef\u901a\u8fc7\u7ebf\u7c92\u4f53\u529f\u80fd\u969c\u788d\u9014\u5f84\u8bf1\u5bfcH446\u7ec6\u80de\u51cb\u4ea1\u3002\u674e\u738b\u5e73\u7b49\uff3b21\uff3d\u53e61\u9879\u7814\u7a76\u5f97\u51fa\uff0c\u767d\u85dc\u82a6\u9187\u53ef\u80fd\u901a\u8fc7PI3K\/\u86cb\u767d\u6fc0\u9176B\uff08Akt\uff09\/\u91cd\u7ec4\u4ebaMyc\u86cb\u767d\uff08c-Myc\uff09\u901a\u8def\u6291\u5236\u4ebaSCLC H446\u7ec6\u80de\u7684\u6d3b\u529b\u5e76\u4fc3\u8fdb\u5176\u51cb\u4ea1\uff0c\u6c27\u5316\u5e94\u6fc0\u548c\u7ebf\u7c92\u4f53\u819c\u7535\u4f4d\u53bb\u6781\u5316\u53ef\u80fd\u53c2\u4e0e\u5176\u8fc7\u7a0b\u3002<\/p>\n

5.2\u3000\u5e72\u6270\u76f8\u5173\u4fe1\u53f7\u8f6c\u5bfc\u901a\u8def<\/h2>\n

\u9ece\u5c55\u534e\u7b49\uff3b22\uff3d\u7814\u7a76\u4e86\u767d\u85dc\u82a6\u9187\u5e72\u9884\u4eba\u80ba\u817a\u764cA549\u7ec6\u80de\uff0c\u5b9e\u9a8c\u5206\u4e3a\u7a7a\u767d\u5bf9\u7167\u7ec4\u3001\u767d\u85dc\u82a6\u9187\u7ec430 \u03bcmol\/L\uff0cmiR-506-\u6291\u5236\u7ec450 \u03bcmol\/L\u53ca\u767d\u85dc\u82a6\u9187\uff0bmiR-506-\u6291\u5236\u7ec4100 \u03bcmol\/L\u5206\u522b\u5e72\u988424\u300148 h\u3002\u7ed3\u679c\u663e\u793a\uff0c\u4e0e\u7a7a\u767d\u7ec4\u6bd4\u8f83\uff0c\u767d\u85dc\u82a6\u9187\u5404\u5e72\u9884\u7ec4\u5747\u80fd\u6291\u5236A549\u7ec6\u80de\u7684\u589e\u6b96\uff0c\u4f7fG0\/G1\u671f\u7684\u7ec6\u80de\u6570\u91cf\u589e\u52a0\uff0cS\u671f\u7684\u7ec6\u80de\u6570\u91cf\u51cf\u5c11\u800c\u8bf1\u5bfc\u5176\u51cb\u4ea1\uff0c\u5176\u4e2d30 \u03bcmol\/L\u767d\u85dc\u82a6\u9187\u5e72\u988424 h\u5bf9A549\u7ec6\u80de\u7684\u6291\u5236\u4f5c\u7528\u6700\u5f31\uff0c100 \u03bcmol\/L\u767d\u85dc\u82a6\u9187\u5e72\u988448 h\u5bf9A549\u7ec6\u80de\u7684\u6291\u5236\u4f5c\u7528\u6700\u660e\u663e\u3002\u6b64\u5916\uff0c\u767d\u85dc\u82a6\u9187\u8fd8\u4e0a\u8c03miR-506\u7684\u8868\u8fbe\u53ca\u4e0b\u8c03\u9798\u6c28\u9187\u6fc0\u91761\u91cd\u7ec4\u86cb\u767d\uff08SPHK1\uff09\u7684\u8868\u8fbe\u3002\u5b9e\u9a8c\u7ed3\u8bba\u5f97\u51fa\uff0c\u767d\u85dc\u82a6\u9187\u901a\u8fc7\u63d0\u9ad8miR-506\u7684\u8868\u8fbe\u4ece\u800c\u6291\u5236SPHK1\u7684\u8868\u8fbe\u8fdb\u800c\u6291\u5236A549\u7ec6\u80de\u7684\u589e\u6b96\u4e14\u8bf1\u5bfc\u5176\u51cb\u4ea1\u3002<\/p>\n

6\u3000\u809d\u764c<\/h2>\n

6.1\u3000\u8c03\u8282\u80bf\u7624\u514d\u75ab\u5fae\u73af\u5883<\/h2>\n

Zhang\u7b49\uff3b23\uff3d\u7814\u7a76\u4eba\u809d\u764c\u7ec6\u80de\u7cfb\u76ae\u4e0bHepa1-6\u548c\u539f\u4f4dH22\u5c0f\u9f20\u6a21\u578b\uff0c\u4f7f\u752850 mg\/kg\u767d\u85dc\u82a6\u9187\u5904\u74063\u5468\u540e\u5206\u6790\uff0c\u5f97\u51fa\u767d\u85dc\u82a6\u9187\u80fd\u591f\u663e\u8457\u6291\u5236\u5c0f\u9f20\u4f53\u5185\u764c\u7ec6\u80de\u7684\u589e\u6b96\u3002\u7ed3\u679c\u663e\u793a\uff0c\u4e0e\u5bf9\u7167\u7ec4\u6bd4\u8f83\uff0c\u767d\u85dc\u82a6\u9187\u5904\u7406\u540e\u7684\u5c0f\u9f20\u80bf\u7624\u4e2d\u51cb\u4ea1\u7ec6\u80de\u6570\u660e\u663e\u589e\u52a0\uff0c\u5c0f\u9f20Hepa1-6\u80bf\u7624\u4e2dp-STAT3\/STAT3\u7684\u8868\u8fbe\u6c34\u5e73\u663e\u8457\u964d\u4f4e\u3002\u9176\u8054\u514d\u75ab\u5438\u9644\u6d4b\u5b9a\uff08ELISA\uff09\u663e\u793a\uff0c\u767d\u85dc\u82a6\u9187\u663e\u8457\u589e\u52a0\u80bf\u7624\u548c\u8840\u6db2\u4e2dTNF-\u03b1\u548c\u4eba\u5e72\u6270\u7d20-\u03b3\uff08IFN-\u03b3\uff09\u7684\u6c34\u5e73\uff0c\u540c\u65f6\u964d\u4f4e\u80bf\u7624\u4e2d\u7684\u767d\u7ec6\u80de\u4ecb\u7d20-10\uff08IL-10\uff09\u6c34\u5e73\uff0c\u8fd9\u8868\u660e\u7528\u767d\u85dc\u82a6\u9187\u53ef\u4ee5\u5bfc\u81f4\u809d\u7ec6\u80de\u764c\u4e2d\u514d\u75ab\u6291\u5236\u6027\u80bf\u7624\u5fae\u73af\u5883\u53d1\u751f\u9006\u8f6c\u3002\u540c\u65f6\uff0c\u5728\u539f\u4f4dH22\u5c0f\u9f20\u6a21\u578b\u4e2d\u53d1\u73b0\uff0c\u767d\u85dc\u82a6\u9187\u5728\u4f53\u5185\u548c\u4f53\u5916\u90fd\u53ef\u4ee5\u901a\u8fc7\u51cf\u5c11CD8+CD122+\u8c03\u8282\u6027T\u7ec6\u80de\uff08Tregs\uff09\u548cM2\u6837\u5de8\u566c\u7ec6\u80de\u53d1\u6325\u6297\u80bf\u7624\u4f5c\u7528\u3002<\/p>\n

6.2\u3000\u8c03\u63a7\u76f8\u5173\u86cb\u767d\u8868\u8fbe<\/h2>\n

\u5b8b\u98de\u51e4\u7b49\uff3b24\uff3d\u4ee5\u4f53\u5916\u57f9\u517b\u7684\u4eba\u809d\u764c\u7ec6\u80deHepG2\u548cHuh7\u4e3a\u7814\u7a76\u5bf9\u8c61\uff0c\u7528\u4e0d\u540c\u6d53\u5ea6\uff086.25\u300125.00\u300150.00\u3001100.00\u3001200.00\u3001250.00 \u00b5mol\/L\uff09\u767d\u85dc\u82a6\u9187\u57f9\u517b24 h\uff0c\u5e76\u786e\u5b9a6.25 \u00b5mol\/L\u4e3a\u8bd5\u9a8c\u6700\u7ec8\u6d53\u5ea6\u3002\u7ed3\u679c\u663e\u793a\uff0c\u767d\u85dc\u82a6\u9187\u53ef\u4ee5\u6291\u5236HepG2\u548cHuh7\u7ec6\u80de\u7684\u4fb5\u88ad\u548c\u8fc1\u79fb\uff0c\u5e76\u4e14\u589e\u52a0\u809d\u764c\u7ec6\u80de\u4e2d\u4e0a\u76ae\u9499\u9ecf\u7d20\u8868\u8fbe\uff0c\u964d\u4f4e\u6ce2\u5f62\u86cb\u767d\u548cTwist1\u8868\u8fbe\uff0c\u63d0\u793a\u767d\u85dc\u82a6\u9187\u6291\u5236\u809d\u764c\u8f6c\u79fb\u53ef\u80fd\u4e0e\u8c03\u63a7EMT\u4fe1\u53f7\u901a\u8def\u6709\u5173\u3002\u540c\u65f6\u5b9e\u9a8c\u53d1\u73b0\uff0c\u767d\u85dc\u82a6\u9187\u80fd\u8bf1\u5bfc\u809d\u764c\u7ec6\u80de\u4e2dmiR-186-5p\u7684\u8868\u8fbe\u3002\u4e0a\u8c03miR-186-5p\u8868\u8fbe\u53ef\u663e\u8457\u6291\u5236\u809d\u764c\u7ec6\u80de\u7684\u8fc1\u79fb\u3001\u4fb5\u88ad\u548cEMT\uff0c\u800c\u6572\u4f4emiR-186-5p\u8868\u8fbe\u80fd\u963b\u65ad\u767d\u85dc\u82a6\u9187\u5bf9\u809d\u764c\u7ec6\u80de\u8fc1\u79fb\u3001\u4fb5\u88ad\u548cEMT\u7684\u6291\u5236\u4f5c\u7528\u3002\u7efc\u4e0a\u6240\u8ff0\uff0c\u767d\u85dc\u82a6\u9187\u53ef\u901a\u8fc7\u4e0a\u8c03miR-186-5p\u8868\u8fbe\u4f53\u5916\u6291\u5236\u809d\u764c\u7ec6\u80de\u8fc1\u79fb\u548c\u4fb5\u88ad\uff0c\u673a\u5236\u53ef\u80fd\u4e0emiR-186-5p\u8c03\u63a7EMT\u76f8\u5173\u86cb\u767d\u8868\u8fbe\u6709\u5173\u3002<\/p>\n

7\u3000\u80c3\u764c<\/h2>\n

Rojo\u7b49\uff3b25\uff3d\u5c06\u4e0d\u540c\u6d53\u5ea6\u7684\u767d\u85dc\u82a6\u9187\u4f5c\u7528\u4e8e\u80c3\u764c\u7ec6\u80de\u7cfbAGS\u548cMKN45\u7ec6\u80de24 h\uff0c\u78fa\u57fa\u7f57\u4e39\u660eB\uff08SRB\uff09\u67d3\u6599\u6cd5\u68c0\u6d4b\u5f97\u51fa\uff0cAGS\u548cMKN45\u7ec6\u80de\u5bf9\u767d\u85dc\u82a6\u9187\u8868\u73b0\u51fa\u660e\u663e\u7684\u654f\u611f\u6027\uff0c\u4f46\u53ea\u6709\u8f83\u9ad8\u5242\u91cf\u7684\u767d\u85dc\u82a6\u918725\uff5e200 \u00b5mol\/L\u624d\u663e\u8457\u6291\u52362\u79cd\u7ec6\u80de\u7684\u7ec6\u80de\u6d3b\u529b\uff0c\u5448\u660e\u663e\u7684\u5242\u91cf\u76f8\u5173\u3002\u6b64\u5916\uff0cBoyden\u5c0f\u5ba4\u6cd5\u663e\u793a\u767d\u85dc\u82a6\u9187\u4ee5\u5242\u91cf\u76f8\u5173\u6027\u65b9\u5f0f\u964d\u4f4eAGS\u548cMKN45\u7ec6\u80de\u7684\u4fb5\u88ad\u529b\uff0c\u4f46\u767d\u85dc\u82a6\u9187\u5728\u8f83\u9ad8\u6d53\u5ea625\uff5e200 \u00b5mol\/L\u4e0b\u5177\u6709\u660e\u663e\u7684\u7ec6\u80de\u6bd2\u6027\u4f5c\u7528\u3002\u5b9e\u9a8c\u8fd8\u663e\u793a\uff0c\u767d\u85dc\u82a6\u9187\u5904\u740624 h\u540e\u660e\u663e\u589e\u52a0\u4e86AGS\u548cMKN45\u7ec6\u80de\u7684SOD\u6d3b\u6027\uff0c\u964d\u4f4e\u4e86NF-\u03baB\u8f6c\u5f55\u6d3b\u6027\u548c\u4e59\u9170\u809d\u7d20\u9176\u7684\u6d3b\u6027\u3002\u4ee5\u4e0a\u7ed3\u679c\u5f97\u51fa\uff0c\u767d\u85dc\u82a6\u9187\u964d\u4f4e\u80c3\u764c\u7ec6\u80de\u7684\u4fb5\u88ad\u80fd\u529b\uff0c\u53ef\u80fd\u4e0e\u589e\u52a0SOD\u6d3b\u6027\u548c\u964d\u4f4eNF-\u03baB\u3001\u4e59\u9170\u809d\u7d20\u9176\u7684\u6d3b\u6027\u6709\u5173\u3002<\/p>\n

\u7814\u7a76\u8bc1\u5b9e\uff0cmiR-155-5p\u7684\u8fc7\u8868\u8fbe\u5bfc\u81f4\u4e86\u80bf\u7624\u7684\u53d1\u751f\u53d1\u5c55\uff3b26\uff3d\u3002Su\u7b49\uff3b27\uff3d\u4ee5\u4eba\u80c3\u764c\u7ec6\u80de\u7cfbSGC7901\u4e3a\u7814\u7a76\u5bf9\u8c61\uff0c\u7528\u4e0d\u540c\u6d53\u5ea6\uff080\u300125\u300150\u3001100\u3001200 \u00b5mol\/L\uff09\u767d\u85dc\u82a6\u9187\u5904\u7406\u3002\u7ed3\u679c\uff0cmiR-155-5p\u5728\u80c3\u764c\u7ec6\u80de\u4e2d\u7684\u8fc7\u5ea6\u8868\u8fbe\uff0840\/49\u4f8b\uff09\uff0c\u4f46\u7ecf\u767d\u85dc\u82a6\u9187\u5904\u7406\u540e\u80c3\u764c\u7ec6\u80de\u751f\u957f\u5f97\u5230\u660e\u663e\u6291\u5236\u3002MTT\u6cd5\u3001\u83cc\u843d\u5f62\u6210\u5b9e\u9a8c\u3001\u7ec6\u80de\u51cb\u4ea1\u5b9e\u9a8c\u7b49\u663e\u793a\uff0c\u767d\u85dc\u82a6\u9187\u663e\u8457\u6291\u5236\u4e86\u80c3\u764c\u7ec6\u80de\u7cfb\u7684\u589e\u6b96\u3001\u4fb5\u88ad\u548c\u8f6c\u79fb\u5e76\u4fc3\u8fdb\u4e86\u764c\u7ec6\u80de\u51cb\u4ea1\u3002\u5b9e\u9a8c\u8fd8\u8bc4\u4f30\u4e86\u95ed\u5408\u86cb\u767d1\uff08Claudin 1\uff09\u548cCaspase-3\u7b49\u9776\u57fa\u56e0\u7ecf\u767d\u85dc\u82a6\u9187\u6cbb\u7597\u540e\u8868\u8fbe\u60c5\u51b5\u3002\u7ed3\u679c\u663e\u793a\uff0c\u767d\u85dc\u82a6\u9187\u663e\u8457\u4e0b\u8c03Claudin1\u3001c-Myc\u3001cyclin D1\u548cBcl-2\u8868\u8fbe\uff0c\u5e76\u4e0a\u8c03\u4e86Caspase-3\u7684\u8868\u8fbe\u3002<\/p>\n

\u603b\u4e4b\uff0c\u767d\u85dc\u82a6\u9187\u6291\u5236\u4e86miR-155-5p\u7684\u8868\u8fbe\uff0c\u5e76\u8fdb\u4e00\u6b65\u5f71\u54cd\u4e86Claudin 1\u3001cyclin D1\u3001Bcl-2\u548cCaspase-3\u86cb\u767d\u7684\u8868\u8fbe\uff0c\u963b\u6b62\u80c3\u764c\u7ec6\u80de\u7684\u751f\u957f\u3001\u4fb5\u88ad\u548c\u8f6c\u79fb\u5e76\u4fc3\u8fdb\u4e86\u5176\u51cb\u4ea1\u3002\u8fd9\u8868\u660e\uff0cmiR-155-5p\u53ef\u80fd\u662f\u80c3\u764c\u6cbb\u7597\u7684\u4e00\u4e2a\u6f5c\u5728\u9776\u70b9\uff0c\u800c\u767d\u85dc\u82a6\u9187\u901a\u8fc7\u5bf9miR-155-5p\u7684\u8c03\u63a7\u53ef\u4ee5\u4f5c\u4e3a\u6f5c\u5728\u7684\u6cbb\u7597\u836f\u7269\u3002<\/p>\n

8\u3000\u80be\u764c\u548c\u5b50\u5bab\u5185\u819c\u764c<\/h2>\n

8.1\u3000\u8c03\u63a7\u76f8\u5173\u86cb\u767d\u8868\u8fbe<\/h2>\n

Tian\u7b49\uff3b28\uff3d\u4ee5\u4eba\u80be\u764c\u7ec6\u80de\u7cfbACHN\u548c786-O\u7ec6\u80de\u4e3a\u7814\u7a76\u5bf9\u8c61\uff0c\u4f7f\u7528\u4e0d\u540c\u6d53\u5ea6\uff0825\u300150\u3001100 \u00b5mol\/L\uff09\u767d\u85dc\u82a6\u9187\u5904\u740612\u300124 h\uff0c\u5e76\u8bbe\u7acb\u5bf9\u7167\u7ec4\u3002\u7ed3\u679c\u8ba4\u4e3a\u767d\u85dc\u82a6\u9187\u663e\u8457\u6291\u5236ACHN\u548c786-O\u7ec6\u80de\u7684\u751f\u957f\uff0c\u4e14\u767d\u85dc\u82a6\u9187\u5728\u8f83\u9ad8\u6d53\u5ea6100 \u00b5mol\/L\u5904\u740624 h\u65f6\u4f1a\u5f15\u8d77\u80be\u764c\u7ec6\u80de\u7684\u5927\u91cf\u6b7b\u4ea1\uff0c\u8868\u73b0\u51fa\u65f6\u95f4\u548c\u6d53\u5ea6\u76f8\u5173\u6027\u3002\u6d41\u5f0f\u7ec6\u80de\u672f\u548c\u86cb\u767d\u8d28\u5370\u8ff9\u3001\u6c27\u6838\u7cd6\u6838\u82f7\u9178\u672b\u7aef\u8f6c\u79fb\u9176\u4ecb\u5bfc\u7684\u7f3a\u53e3\u672b\u7aef\u6807\u8bb0\u6cd5\uff08TUNEL\uff09\u6d4b\u5b9a\u663e\u793a\uff0c\u4e0e\u5bf9\u7167\u7ec4\u6bd4\u8f83\uff0c\u767d\u85dc\u82a6\u9187\u6cbb\u7597\u5728\u4f53\u5916\u548c\u4f53\u5185\u80fd\u663e\u8457\u8bf1\u5bfcACHN\u548c786-O\u7ec6\u80de\u7684\u51cb\u4ea1\u3002<\/p>\n

\u4f24\u53e3\u6108\u5408\u548cTranswell\u4fb5\u88ad\u5b9e\u9a8c\u663e\u793a\uff0c\u767d\u85dc\u82a6\u9187\u80fd\u660e\u663e\u6291\u5236\u80be\u764c\u7ec6\u80de\u7684\u8fc1\u79fb\u548c\u4fb5\u88ad\u3002\u514d\u75ab\u7ec4\u5316\u548cWestern blotting\u68c0\u6d4b\u5f97\u51fa\uff0c\u767d\u85dc\u82a6\u9187\u80fd\u964d\u4f4e\u80be\u764c\u7ec4\u7ec7\u4e2d\u542bNLR\u5bb6\u65cfPyrin\u57df\u86cb\u767d3\uff08NLRP3\uff09\u7684\u8868\u8fbe\u3002\u4ee5\u4e0a\u7ed3\u679c\u8868\u660e\uff0c\u767d\u85dc\u82a6\u9187\u901a\u8fc7\u4e0b\u8c03NLRP3\u7684\u8868\u8fbe\u5728\u4f53\u5185\u4f53\u5916\u6291\u5236\u80be\u764c\u7ec6\u80de\u7684\u751f\u957f\u3001\u4fb5\u88ad\u548c\u8fc1\u79fb\uff0c\u5e76\u589e\u52a0\u764c\u7ec6\u80de\u7684\u51cb\u4ea1\uff0cNLRP3\u53ef\u80fd\u662f\u80be\u7ec6\u80de\u764c\u6cbb\u7597\u7684\u6f5c\u5728\u5206\u5b50\u9776\u70b9\u3002<\/p>\n

8.2\u3000\u5e72\u6270\u76f8\u5173\u4fe1\u53f7\u8f6c\u5bfc\u901a\u8def<\/h2>\n

\u8303\u61ff\u96bd\u7b49\uff3b29\uff3d\u7528\u4e0d\u540c\u6d53\u5ea6\uff080\u300125\u300150\u3001100\u3001200 \u00b5mol\/L\uff09\u767d\u85dc\u82a6\u9187\u5206\u522b\u5904\u7406\u5b50\u5bab\u5185\u819c\u764cIshikawa\u7ec6\u80de24\u300148\u300172 h\u3002\u7ed3\u679c\u663e\u793a\uff0c\u767d\u85dc\u82a6\u9187\u5bf9Ishikawa\u7ec6\u80de\u7684\u589e\u6b96\u6709\u6291\u5236\u4f5c\u7528\uff0c\u5e76\u4e14\u5728\u6d53\u5ea6\u4e3a100 \u00b5mol\/L\u57f9\u517b24 h\u6291\u5236\u7387\u6700\u9ad8\u3002Western blotting\u68c0\u6d4b\u53d1\u73b0\uff0c\u767d\u85dc\u82a6\u9187\u80fd\u591f\u4e0a\u8c03\u81ea\u566c\u76f8\u5173\u86cb\u767dBeclin1\u548cLC3mRNA\u7b49\u6c34\u5e73\uff0c\u4e0b\u8c03\u78f7\u8102\u9170\u808c\u91873-\u6fc0\u9176\uff08p-PI3K\uff09\u548c\u78f7\u9178\u5316\u86cb\u767d\u6fc0\u9176\u86cb\u767d\uff08p-Akt\uff09\u6c34\u5e73\u3002\u7ec6\u80de\u514d\u75ab\u8367\u5149\u68c0\u6d4b\u53d1\u73b0\uff0c\u767d\u85dc\u82a6\u9187\u4f5c\u7528\u4e8eIshikawa\u7ec6\u80de\u540e\uff0c\u7ec6\u80de\u8d28LC3\u7ea2\u8272\u8367\u5149\u5f3a\u5ea6\u589e\u5f3a\uff0c\u81ea\u566c\u5c0f\u4f53\u6570\u91cf\u589e\u52a0\uff0c\u63d0\u793a\u81ea\u566c\u53ef\u80fd\u53c2\u4e0e\u4e86\u767d\u85dc\u82a6\u9187\u7684\u6297\u80bf\u7624\u4f5c\u7528\uff0c\u800cPI3K\/Akt\u4fe1\u53f7\u901a\u8def\u4e5f\u4f1a\u6291\u5236\u81ea\u566c\u7684\u53d1\u751f\uff3b30\uff3d\u3002\u56e0\u6b64\uff0c\u8be5\u7814\u7a76\u8ba4\u4e3a\u767d\u85dc\u82a6\u9187\u53ef\u80fd\u901a\u8fc7\u6291\u5236PI3K\/Akt\u4fe1\u53f7\u901a\u8def\uff0c\u4fc3\u8fdb\u5b50\u5bab\u5185\u819c\u764c\u7ec6\u80de\u53d1\u751f\u81ea\u566c\uff0c\u4ece\u800c\u5bfc\u81f4\u589e\u6b96\u5f97\u5230\u6291\u5236\u3002<\/p>\n

9\u3000\u7ed3\u8bed\u4e0e\u5c55\u671b<\/h2>\n

\u7efc\u4e0a\u6240\u8ff0\uff0c\u767d\u85dc\u82a6\u9187\u6297\u80bf\u7624\u8c31\u5e7f\uff0c\u5728\u591a\u79cd\u80bf\u7624\u7ec6\u80de\u4e2d\u5747\u8868\u73b0\u51fa\u663e\u8457\u7684\u6297\u80bf\u7624\u6d3b\u6027\uff0c\u53ef\u901a\u8fc7\u591a\u79cd\u4fe1\u53f7\u901a\u8def\u53ca\u8c03\u63a7\u76f8\u5173\u86cb\u767d\u8868\u8fbe\u7b49\u6291\u5236\u764c\u7ec6\u80de\u751f\u957f\u3001\u589e\u6b96\u3001\u4fb5\u88ad\u548c\u8f6c\u79fb\u3001\u8bf1\u5bfc\u7ec6\u80de\u51cb\u4ea1\uff0c\u5e76\u80fd\u591f\u9006\u8f6c\u5316\u7597\u8010\u836f\u6027\u53ca\u589e\u5f3a\u5316\u7597\u654f\u611f\u6027\uff0c\u5df2\u7ecf\u6210\u4e3a\u5f53\u524d\u6297\u764c\u65b0\u836f\u7814\u53d1\u7684\u6f5c\u5728\u70ed\u70b9\u836f\u7269\u4e4b\u4e00\u3002<\/p>\n

\u76ee\u524d\u5bf9\u4e8e\u767d\u85dc\u82a6\u9187\u6297\u80bf\u7624\u836f\u7406\u4f5c\u7528\u7684\u53d1\u6325\u53ca\u673a\u5236\u7814\u7a76\u5c1a\u5b58\u6709\u4e0d\u8db3\u4e4b\u5904\uff1a<\/p>\n

\uff081\uff09\u767d\u85dc\u82a6\u9187\u5728\u53d1\u6325\u6297\u80bf\u7624\u4f5c\u7528\u65f6\u5448\u660e\u663e\u7684\u65f6\u95f4\u548c\u6d53\u5ea6\u76f8\u5173\u6027\uff0c\u4f46\u5f53\u8fbe\u4e0d\u5230\u6240\u9700\u7684\u65f6\u95f4\u6216\u5242\u91cf\u65f6\u5176\u6297\u80bf\u7624\u4f5c\u7528\u8868\u73b0\u4e0d\u8db3\uff0c\u5927\u5242\u91cf\u65f6\u5219\u4f1a\u51fa\u73b0\u7ec6\u80de\u6bd2\u6027\uff0c\u6240\u4ee5\u9700\u8981\u66f4\u591a\u7684\u5927\u578b\u968f\u673a\u5bf9\u7167\u8bd5\u9a8c\u53bb\u8fdb\u4e00\u6b65\u786e\u5b9a\u5176\u6709\u6548\u5242\u91cf\u53ca\u5b89\u5168\u6027\uff1b<\/p>\n

\uff082\uff09\u767d\u85dc\u82a6\u9187\u5b58\u5728\u751f\u7269\u5229\u7528\u7387\u4f4e\u3001\u4ee3\u8c22\u5feb\u53ca\u4f53\u5185\u5206\u5e03\u6709\u9650\u7684\u95ee\u9898\uff0c\u672a\u6765\u5e94\u8be5\u628a\u91cd\u70b9\u653e\u5728\u7814\u53d1\u5236\u5907\u767d\u85dc\u82a6\u9187\u4e0e\u5176\u4ed6\u7269\u8d28\u7ed3\u5408\u7269\u6216\u53ef\u8d1f\u8f7d\u767d\u85dc\u82a6\u9187\u7684\u53ef\u751f\u7269\u964d\u89e3\u7684\u7eb3\u7c73\u6750\u6599\u65b9\u9762\u63d0\u9ad8\u5176\u751f\u7269\u5229\u7528\u5ea6\uff0c\u4f8b\u5982Johnson\u7b49\uff3b31\uff3d\u7814\u7a76\u4e86\u80e1\u6912\u78b1\u4e0e\u767d\u85dc\u82a6\u9187\u8054\u5408\u7ed9\u836f\u5bf9C57BL\u5c0f\u9f20\u8840\u6e05\u767d\u85dc\u82a6\u9187\u548c\u767d\u85dc\u82a6\u9187-3-O-\u03b2-D-\u8461\u7cd6\u82f7\u9178\u6c34\u5e73\u7684\u5f71\u54cd\uff0c\u7ed3\u679c\u53d1\u73b0\u80e1\u6912\u78b1\u901a\u8fc7\u6291\u5236\u5176\u8461\u8404\u7cd6\u919b\u9178\u5316\u589e\u5f3a\u4e86\u767d\u85dc\u82a6\u9187\u7684\u836f\u52a8\u5b66\u53c2\u6570\u3001\u51cf\u7f13\u5176\u4f53\u5185\u6d88\u9664\u901f\u5ea6\uff0c\u4ece\u800c\u63d0\u9ad8\u4e86\u751f\u7269\u5229\u7528\u5ea6\uff1b<\/p>\n

\uff083\uff09\u76ee\u524d\u5bf9\u767d\u85dc\u82a6\u9187\u6297\u80bf\u7624\u673a\u5236\u7684\u7814\u7a76\u591a\u4ece1\u79cd\u6216\u51e0\u79cd\u673a\u5236\u9610\u8ff0\uff0c\u5c1a\u7f3a\u4e4f\u5b8c\u5584\u7684\u6297\u80bf\u7624\u7cfb\u7edf\u7f51\u7edc\uff0c\u672a\u6765\u5e94\u8be5\u5229\u7528\u73b0\u4ee3\u6280\u672f\u624b\u6bb5\u5c06\u9ad8\u4ef7\u503c\u7684\u4e34\u5e8a\u8bd5\u9a8c\u7ed3\u679c\u6536\u96c6\u8d77\u6765\u7efc\u5408\u5206\u6790\u5176\u4f5c\u7528\u673a\u5236\uff0c\u4e3a\u4e34\u5e8a\u65b0\u836f\u7684\u5f00\u53d1\u63d0\u4f9b\u66f4\u53ef\u4fe1\u7684\u6570\u636e\u652f\u6301\uff0c\u4ece\u800c\u4fc3\u8fdb\u767d\u85dc\u82a6\u9187\u76f8\u5173\u5236\u5242\u7684\u4e34\u5e8a\u4f7f\u7528\uff0c\u4ee5\u4f7f\u66f4\u591a\u7684\u764c\u75c7\u60a3\u8005\u53d7\u76ca\u3002<\/p>\n

\u5229\u76ca\u51b2\u7a81 \u6240\u6709\u4f5c\u8005\u5747\u58f0\u660e\u4e0d\u5b58\u5728\u5229\u76ca\u51b2\u7a81<\/p>\n

\u53c2\u8003\u6587\u732e\uff08\u7565\uff09<\/p>\n

\u6765 \u6e90\uff1a\u738b\u771f\uff0c\u9648\u7acb\u4f1f\uff0e\u767d\u85dc\u82a6\u9187\u6297\u80bf\u7624\u836f\u7406\u4f5c\u7528\u673a\u5236\u7814\u7a76\u8fdb\u5c55 [J]. \u836f\u7269\u8bc4\u4ef7\u7814\u7a76, 2023, 46(2): 245-251 .<\/p>\n","protected":false},"excerpt":{"rendered":"

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