{"id":33537,"date":"2024-05-13T00:00:54","date_gmt":"2024-05-13T00:00:54","guid":{"rendered":"https:\/\/zydq.1006ss.com\/?p=33537"},"modified":"2024-05-13T00:00:54","modified_gmt":"2024-05-13T00:00:54","slug":"%e8%bf%9e%e7%bf%98%e6%88%90%e5%88%86%e3%80%81%e8%8d%af%e7%90%86%e3%80%81%e4%b8%b4%e5%ba%8a%e5%8f%8a%e5%ae%89%e5%85%a8%e6%80%a7%e7%bb%bc%e8%bf%b0","status":"publish","type":"post","link":"http:\/\/zydq.1006ss.com\/?p=33537","title":{"rendered":"\u8fde\u7fd8\u6210\u5206\u3001\u836f\u7406\u3001\u4e34\u5e8a\u53ca\u5b89\u5168\u6027\u7efc\u8ff0"},"content":{"rendered":"

\u6587\u6863<\/p>\n

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\u8fde\u7fd8\u4e3a\u6728\u7280\u79d1\u8fde\u7fd8\u5c5e\u690d\u7269\u8fde\u7fd8[Forsythia suspensa\uff08Thunb.\uff09Vahl]\u5176\u5e72\u71e5\u679c\u5b9e\uff0c\u522b\u540d\uff1a\u8fde\u3001\u5f02\u7fd8\u3001\u5170\u534e\u3001\u8f75\u3001\u6298\u6839\u3001\u4e09\u5ec9\u3001\u8fde\u8349\u3001\u5927\u7fd8\u3001\u9ec4\u82b1\u6746\u3001\u9ec4\u5bff\u4e39\u3001\u9ec4\u82b1\u74e3\u3001\u843d\u7fd8\u3001\u9ec4\u82b1\u6761\u3001\u8fde\u58f3\u3001\u9ec4\u94fe\u6761\u82b1\u3001\u9ec4\u5947\u4e39\u3001\u9752\u7fd8\u3002\u6700\u65e9\u8f7d\u4e8e\u300a\u795e\u519c\u672c\u8349\u7ecf\u300b\u4e0b\u54c1\uff1a\u201c\u8fde\u7fd8\u3002\u5473\u82e6\uff0c\u5e73\u3002\u4e3b\u5bd2\u70ed\u9f20\u7618\uff0c\u7630\u75ac\u75c8\u80bf\uff0c\u6076\u75ae\u763f\u7624\uff0c\u7ed3\u70ed\u86ca\u6bd2\u3002\u4e00\u540d\u5f02\u7fd8\uff0c\u4e00\u540d\u5170\u534e\uff0c\u4e00\u540d\u8f75\uff0c\u4e00\u540d\u4e09\u5ec9\u3002\u751f\u5c71\u8c37\u201d\u3002\u4f20\u7edf\u4e2d\u533b\u8ba4\u4e3a\u8fde\u7fd8\u5473\u82e6\uff0c\u6027\u5fae\u5bd2\u3002\u5165\u5fc3\u3001\u809d\u3001\u80c6\u7ecf\u3002\u5177\u6709\u6e05\u70ed\u89e3\u6bd2\u3001\u6d88\u80bf\u6563\u7ed3\u3001\u758f\u6563\u98ce\u70ed\u4e4b\u529f\u6548\uff0c\u7528\u4e8e\u6cbb\u7597\u75c8\u75bd\u3001\u7630\u75ac\u3001\u4e73\u75c8\u3001\u4e39\u6bd2\u3001\u98ce\u70ed\u611f\u5192\u3001\u6e29\u75c5\u521d\u8d77\u3001\u6e29\u70ed\u5165\u8425\u3001\u9ad8\u70ed\u70e6\u6e34\u3001\u795e\u660f\u53d1\u6591\u3001\u70ed\u6dcb\u6da9\u75db\u7b49\u8bc1\u3002\u4e3b\u8981\u6210\u5206\u542b\u6709\u6325\u53d1\u6cb9\u7c7b\u3001\u82ef\u4e59\u9187\u82f7\u7c7b\u3001\u6728\u8102\u7d20\u7c7b\u548c\u4e09\u841c\u7c7b\u7269\u8d28\u7b49\uff0c\u4ece\u4e2d\u5206\u5f97\u7684\u9ec4\u916e\u7c7b\u7269\u8d28\u8f83\u5c11\uff0c\u915a\u9178\u7c7b\u7269\u8d28\u5219\u8f83\u591a\u3002\u836f\u7406\u4f5c\u7528\u4e3a\u6297\u6c27\u5316\u3001\u6297\u83cc\u3001\u6297\u708e\u3001\u6297\u75c5\u6bd2\u3001\u6297\u80bf\u7624\u3001\u6297\u8fc7\u654f\u4ee5\u53ca\u89e3\u70ed\u9547\u75db\u7b49\u3002\u8fd1\u5e74\u6765\uff0c\u968f\u7740\u4e2d\u836f\u73b0\u4ee3\u5316\u7684\u4e0d\u65ad\u6df1\u5165\uff0c\u7279\u522b\u662f\u8bf8\u5982\u201c\u7532\u6d41\u201d\u201c\u65b0\u51a0\u201d\u7b49\u7a81\u53d1\u6027\u516c\u5171\u536b\u751f\u4e8b\u4ef6\u4e0d\u65ad\u51fa\u73b0\uff0c\u6380\u8d77\u4e86\u672c\u690d\u7269\u53ca\u5176\u540c\u5c5e\u690d\u7269\u7684\u5316\u5b66\u7814\u7a76\u9ad8\u6f6e\u3002<\/p>\n

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\u53e6\u6709\u8d2f\u53f6\u8fde\u7fd8(Hypericum peforamm1)\uff0c\u53c8\u540d\u8d2f\u53f6\u91d1\u4e1d\u6843\u3001\u5343\u5c42\u697c\u3001\u5723\u7ea6\u7ff0\u8349(St\uff0eJohn\u2019s wort)\uff0c\u662f\u85e4\u9ec4\u79d1\u91d1\u4e1d\u6843\u5c5e\u690d\u7269\u3002\u968f\u7740\u5bf9\u5176\u836f\u7406\u6d3b\u6027\u7814\u7a76\u7684\u6df1\u5165\uff0c\u8d2f\u53f6\u8fde\u7fd8\u8d8a\u6765\u8d8a\u53d7\u5230\u4eba\u4eec\u7684\u91cd\u89c6\u3002\u8d2f\u53f6\u8fde\u7fd8\u7684\u4e3b\u8981\u6d3b\u6027\u6210\u5206\u9ec4\u916e\u8d2f\u53f6\u91d1\u4e1d\u6843\u7d20\u3001\u91d1\u4e1d\u6843\u7d20\u3001\u91d1\u4e1d\u6843\u82f7\u7b49\u53ca\u8418\u9a88\u4e8c\u84bd\u916e\u7c7b\u3001\u95f4\u82ef\u4e09\u915a\u7c7b\u3001\u6325\u53d1\u6cb9\u3001\u97a3\u8d28\u3001\u539f\u82b1\u9752\u7d20\u3001\u80e1\u841d\u535c\u7d20\u3001\u03b2-\u8c37\u753e\u9187\u3001\u5f02\u7f2c\u8349\u9178\u3001\u6708\u6842\u9178\u548c\u8089\u8c46\u853b\u9178\u7b49\u6210\u5206\u3002\u5177\u6709\u6297\u6291\u90c1\u3001\u6297\u75c5\u6bd2\u3001\u6297\u80bf\u7624\u3001\u6297\u708e\u3001\u6b62\u54b3\u3001\u9547\u75db\u7b49\u4f5c\u7528\u3002\u76ee\u524d\uff0c\u7528\u8d2f\u53f6\u8fde\u7fd8\u63d0\u53d6\u7269\u5236\u6210\u7684\u5236\u5242\u5df2\u5728\u6b27\u7f8e\u5927\u91cf\u4e0a\u5e02\uff0c\u4e3b\u8981\u7528\u4e8e\u6cbb\u7597\u6291\u90c1\u75c7\u3001\u7532\u578b\u548c\u4e59\u578b\u809d\u708e\u53ca\u827e\u6ecb\u75c5\u7b49\u3002\u6b64\u5916\uff0c\u8fd8\u6709\u6587\u732e\u62a5\u9053\u5176\u6709\u6297\u80bf\u7624\u6d3b\u6027\u3002\u76ee\u524d\u8d2f\u53f6\u8fde\u7fd8\u5728\u56fd\u5916\u7814\u7a76\u8f83\u591a\uff0c\u800c\u56fd\u5185\u7814\u7a76\u751a\u5c11\u3002\u4f46\u662f\u8d2f\u53f6\u8fde\u7fd8\u5728\u4e2d\u56fd\u5177\u6709\u5f88\u957f\u7684\u836f\u7528\u5386\u53f2\uff0c\u6839\u636e\u4f20\u7edf\u4e2d\u533b\u7406\u8bba\uff0c\u8d2f\u53f6\u8fde\u7fd8\u5473\u8f9b\u82e6\u6da9\uff0c\u5176\u6027\u5e73\uff0c\u5177\u6709\u6e05\u5fc3\u660e\u76ee\u3001\u8212\u7ecf\u6d3b\u8840\u3001\u6b62\u8840\u751f\u808c\u3001\u89e3\u6bd2\u6d88\u708e\u3001\u5229\u6e7f\u4e4b\u529f\u6548\u3002\u4e0e\u8fde\u7fd8\u5e94\u7528\u6709\u522b\u3002<\/p>\n

1. \u5316\u5b66\u6210\u5206<\/p>\n

\u8fd15\u5e74\u6765\uff0c\u4ece\u8fde\u7fd8\u4e2d\u5206\u5f97\u7684\u5316\u5b66\u6210\u5206\u4e3b\u8981\u6709\u6325\u53d1\u6cb9\u7c7b\u3001\u82ef\u4e59\u9187\u82f7\u7c7b\u3001\u6728\u8102\u7d20\u7c7b\u548c\u4e09\u841c\u7c7b\u7269\u8d28\uff0c\u4ece\u4e2d\u5206\u5f97\u7684\u9ec4\u916e\u7c7b\u7269\u8d28\u8f83\u5c11\uff0c\u915a\u9178\u7c7b\u7269\u8d28\u5219\u6bd4\u4e4b\u524d\u8f83\u591a\u3002<\/p>\n

1.1 \u6325\u53d1\u6027\u6210\u5206<\/p>\n

\u8fde\u7fd8\u4e2d\u7684\u6325\u53d1\u6027\u6210\u5206\u542b\u91cf\u5f88\u9ad8\uff0c\u5728\u8fde\u7fd8\u7684\u79cd\u5b50\u8fde\u7fd8\u5fc3\u4e2d\u542b\u91cf\u5e73\u5747\u53ef\u8fbe3.8\uff05\uff0c\u800c\u5728\u81ea\u7136\u754c\u4e2d\u542b\u6325\u53d1\u6cb9\u5982\u6b64\u9ad8\u7684\u690d\u7269\u5e76\u4e0d\u591a\u89c1\u30022009 \u5e74\u8303\u4ee4\u521a\u7b49\u4ece\u8fde\u7fd8\u82b1\u4e2d\u9274\u5b9a\u51fa24 \u79cd\u6325\u53d1\u6cb9\u7c7b\u6210\u5206\uff0c\u4e3b\u8981\u662f\u4fa7\u67cf\u70ef\u3001\u03b2-\u6c34\u82b9\u70ef\u3001\u03b2-\u848e\u70ef\u3001\u6849\u6cb9\u7cbe\u30013-\u5f02\u4e19\u70ef\u57fa-5\uff0c5-\u4e8c\u7532\u57fa\u73af\u620a\u70ef\u3001\u03b2-\u6708\u6842\u70ef\u3001\u6c34\u6768\u9178\u7532\u916f\u7b49\u3002<\/p>\n

\u53e6\u6709\u62a5\u9053\u542b\uff1a155 \u03b1-\u848e\u70ef(alpha-Pinene)\u3001156 \u03b2-\u848e\u70ef(beta-Pinene)\u3001157\u9a6c\u97ad\u8349\u70ef\u916e(Verbenone)\u3001158 \u6843\u91d1\u5a18\u70ef\u919b\uff3b(-)-Myrtenal\uff3d\u3001159 b-\u4e59\u9178\u677e\u6cb9\u916f(b-Terpinyl acetate)\u3001160 3-\u5f02\u4e19\u57fa\u7532\u82ef(p Isopropyltoluene)\u3001161 \u03b3-\u841c\u54c1\u70ef(gamma-Terpinene)\u3001162 \u03b2-\u6c34\u82b9\u70ef(beta-Phellandrene)\u3001163\u03b1-\u841c\u54c1\u6cb9\u70ef(\u03b1-Terpinolene)\u3001164\u03b1-\u6c34\u82b9\u70ef(\u03b1-Phellandrene)\u3001165\u677e\u6cb9\u70ef-4-\u9187(Terpinen-4-ol)\u3001166\u677e\u6cb9\u70ef(Terpinene)\u3001167\u7f57\u52d2\u70ef(Ocimene)\u3001168\u03b1-\u677e\u6cb9\u9187(\u03b1-Terpineol)\u3001169\u68d5\u6988\u9178(Palmitic
\nacid)\u3001170\u9999\u82b9\u8349\u5b5f\u70ef\u9187(4-Carvomenthenol)\u3001171\u67e0\u6aac\u70ef(Limonene)\u3001172\u82b3\u6a1f\u9187(Linalool)\u3001173\u6a1f\u8111(Camphor)\u3001174 2\uff0c6-\u4e8c\u53d4\u4e01\u57fa\u5bf9\u7532\u915a(2\uff0c6-Di-tert-butyl-4-methyl-phenol)\u3001175 2\uff0c6-\u4e8c\u53d4\u4e01\u57fa-4-(1-\u6c27\u4ee3\u4e19\u57fa)\u82ef\u915a\uff3b2\uff0c6-Bis(1\uff0c1-dimethylethyl)-4-(1-oxopropyl)phenol\uff3d\u3001176\u77f3\u7af9\u70ef(Caryophyllene)\u3001177\u9999\u53f6\u9187(Geraniol)\u3001178\u5bf9\u4f1e\u82b1\u70c3(p-Cymene)\u3001179\u03b2-\u6708\u6842\u70ef(\u03b2-Myrcene)\u3001180\u4e59\u9178\u7fbd\u6247\u70ef\u9178\u916f(Lupenylacetate)\u3001181\u58ec\u919b(Nonanal)\u3001182 3-\u7532\u57fa-1\uff0c2-\u73af\u620a\u70f7\u4e8c\u916e(3-methyl-1\uff0c2-Cyclopen-tanedione)\u3001183\u5341\u4e94\u70f7(Pentadecane)\u3001184\u5341\u516d\u70f7(Hexadecane)\u3001185\u8089\u8c46\u853b\u9178(Tetradecanoic acid)\u3001186 6\uff0c10\uff0c14-\u4e09\u7532\u57fa-2-\u5341\u4e94\u70f7\u916e(6\uff0c10\uff0c14-trimethyl-2-Pentadecanone)\u3001187\u4e8c\u5341\u4e09\u70f7(Tricosane)\u3001189\u03b2-\u4f1e\u82b1\u70c3(\u03b2-Cymene)\u3001190\u679e\u6cb9\u70ef(Sylvestrene)\u3001191\u9999\u6867\u70ef(Sabinene)\u3001192\u03b2-\u6708\u6842\u70ef(beta-Myrcene)\u3001193\u5df2\u919b(Heptanal)\u3001194\u73af\u5df1\u70f7(Hexamethylene)\u3001195 2\uff0c3\uff0c5\uff0c6-tetrahydro-jacara-none-4-O-\u03b2-D-glucopy-ranoside\u3001196\u83b0\u70ef(Camphene)\u3001197\u9999\u8349\u919b(Vanillin)\u3001198 1-\u7532\u57fa-2-\u5f02\u4e19\u57fa\u82ef(1-Methyl-2-isopropylbenzene)\u3001199\u82ef\u7532\u9187(Benzyl alcohol)\u3001200\u82ef\u4e59\u9187(Phenylethyl alcohol)\u3001201\u8451\u9187(Fenchol)\u3001202\u51b0\u7247(Borneol)\u3001203\u841c\u54c1\u9187-4(Terpineol-4)\u3001204 2-\u7f9f\u57fa\u6849\u6811\u8111(2-Hydroxycineole)\u3001205 1-\u7532\u57fa-4-\u5f02\u4e19\u57fa\u82ef(1-Methyl-4-isopropylbenzene)\u3001206\u5bf9-\u5f02\u4e19\u57fa\u82ef\u7532\u9187(p-Isopropylbenzyl alcohol)\u3001207 2\uff0c4-\u7678\u4e8c\u70ef\u919b(2\uff0c4-Decadienal)\u3001208\u03b3-\u675c\u677e\u70ef(\u03b3-Cadinene)\u3001209\u03b4-\u675c\u677e\u70ef(\u03b4-Cadinene)\u3001210 19-hydroxy-13-oxo-15\uff0c16-dinor-ent-labda-8(17)-ene\u3001211 18-hydroxy-13-oxo-15\uff0c16-dinor-cleroda-3\uff0c4-ene\u3001212 3\uff0c13-oxo-15\uff0c16-dinor-ent-labda-8(17)-ene\u3001213 serralabdane B\u3001214(4S\uff0c9\uff32\uff0c10\uff32)methyl 18-carboxy-labda-8\uff0c13(E)-diene-15-oate\u3002<\/p>\n

1.2 \u82ef\u4e59\u9187\u82f7\u7c7b<\/p>\n

\u4e4b\u524d\u6709\u62a5\u9053\u8fde\u7fd8\u4e2d\u542b\u6709\u5927\u91cf\u7684\u82ef\u4e59\u9187\u82f7\u7c7b\u6210\u5206\uff0c\u8be5\u7c7b\u5316\u5408\u7269\u662f\u8fde\u7fd8\u4e2d\u4e3b\u8981\u7684\u6d3b\u6027\u6210\u5206\uff0c\u4e3b\u8981\u6709\u8fde\u7fd8\u916f\u82f7A\uff08forshytosideA\uff09\u3001\u8fde\u7fd8\u916f\u82f7C\uff08forshytoside C\uff09\u3001\u8fde\u7fd8\u916f\u82f7D\uff08forshytosideD\uff09\u3001\u6cfd\u4e01\u9999\u915a\u82f7\uff08acteoside\uff09\u3001\u03b2-\u7f9f\u57fa\u6cfd\u4e01\u9999\u915a\u82f7\uff08\u03b2-hydorxyacteoside\uff09\u3001\u8fde\u7fd8\u916f\u82f7B\uff08forshytoside B\uff09\u548c\u8fde\u7fd8\u916f\u82f7E\uff08forshytosideE\uff09\u7b49\u30022008 \u5e74\uff0c\u66f2\u6b22\u6b22\u4ece\u8fde\u7fd8\u679c\u5b9e\u4e2d\u5206\u79bb\u5f97\u5230\u4e86\u4e00\u79cd\u65b0\u7684\u82ef\u4e59\u9187\u82f7\u7c7b\u6210\u5206\u5f02\u8fde\u7fd8\u916f\u82f7\uff0c\u5b83\u662f\u8fde\u7fd8\u916f\u82f7\u4e2d\u03b1\u578b\u9f20\u674e\u7cd6\u6784\u578b\u8f6c\u53d8\u4e3a\u03b2\u578b\u7684\u8fde\u7fd8\u916f\u82f7\u5f02\u6784\u4f53\u3002\u51af\u536b\u751f\u7814\u7a76\u7ec4\u4ece\u8fde\u7fd8\u4e2d\u5206\u5f97\u4e00\u4e2a\u65b0\u7684\u8fde\u7fd8\u916f\u82f7forsythenside F\uff0c2009 \u5e74\u8be5\u7814\u7a76\u7ec4\u53c8\u9996\u6b21\u4ece\u8fde\u7fd8\u4e2d\u5206\u5f972 \u4e2a\u82ef\u4e59\u9187\u82f7\u7c7b\u5316\u5408\u7269\uff1a\u6728\u901a\u82ef\u4e59\u9187\u82f7B\u548c3\uff0c4-\u4e8c\u7f9f\u57fa\u82ef\u4e59\u57fa-8-O-\u03b2-D-\u8461\u8404\u7cd6\u82f7\u3002<\/p>\n

\u6709\u62a5\u9053\u542b1\u03b2-D-glucopyranside\u30012 3\uff0c4-\u4e8c\u7f9f\u57fa\u82ef\u4e59\u57fa-8-O-\u03b2-D-\u8461\u8404\u7cd6\u30013\u8fde\u7fd8\u916f\u82f7A(forsythoside A)\u30014\u8fde\u7fd8\u916f\u82f7B(forsythoside B)\u30015\u8fde\u7fd8\u916f\u82f7C(forsythoside C)(suspenside)\u30016\u8fde\u7fd8\u916f\u82f7D(forsythoside
\nD)\u30017\u8fde\u7fd8\u916f\u82f7E(forsythoside E)\u30018\u8fde\u7fd8\u916f\u82f7F(forsythoside F)\u30019\u8fde\u7fd8\u916f\u82f7G(forsythoside G)\u300110\u8fde\u7fd8\u916f\u82f7H(forsythoside
\nH)\u300111\u8fde\u7fd8\u916f\u82f7I(forsythoside I)\u300112\u8fde\u7fd8\u916f\u82f7J(forsythoside J)\u300113\u8fde\u7fd8\u916f\u82f7K(forsythoside K)\u300114\u5de6\u65cb\u7f9f\u57fa\u8fde\u7fd8\u916f\u82f7A\uff3b(-)-suspensaside A\uff3d\u300115\u5de6\u65cb\u7f9f\u57fa\u8fde\u7fd8\u916f\u82f7B\uff3b(-)-suspensaside B\uff3d\u300116 2-(3\uff0c4-\u4e8c\u7f9f\u57fa\u82ef\u57fa)-\u4e59\u57fa-\u03b2-D-\u8461\u8404\u7cd6\u82f7\uff3b2-(3\uff0c 4-dihydroxyphenyl)ethyl-\u03b2-D-glucopyranoside\uff3d\u300117\u82ef\u4e59\u9187-\u03b2-D-\u6728\u7cd6\u57fa-(1\u21926)-\u03b2-D-\u8461\u8404\u7cd6\u82f7\uff3bPhenethylalcohol-\u03b2-D-xylopyranosy-(1\u21926)-\u03b2-D-glucopyranoside\uff3d\u300118 7S-suspensaside methyl
\nether\u300119\u8fde\u7fd8\u65b0\u82f7B(lianqiaoxinside B)\u300120\u6bdb\u67f3\u82f7(salidroside)\u300121\u6728\u901a\u82ef\u4e59\u9187\u82f7A(calceolarioside A)\uff3b13\uff3d 22\u6728\u901a\u82ef\u4e59\u9187\u82f7B(calceolarioside B)\u300123\u8f66\u524d\u8349\u82f7A(plantainoside A)\uff3b13\uff3d 24\u8f66\u524d\u8349\u82f7B(plantainoside B)\u300125\u6cfd\u4e01\u9999\u915a\u82f7(Acteoside)\u300126\u03b2-\u7f9f\u57fa\u6cfd\u4e01\u9999\u915a\u82f7(\u03b2-hydroxyacteoside)\u300127\uff32-Suspensaside\u300128 S-Suspensaside\u300129 S-Suspensaside menthy ether\u300130\u8fde\u7fd8\u65b0\u82f7A(Lianqiaoxinside A)\u300131\u8fde\u7fd8\u65b0\u82f7C(Lianqiaoxinside C)\u300132\u6728\u901a\u82ef\u4e59\u9187\u82f7C(calceolarioside C)\u300133\u03b2-(3\uff0c4-dihydr-oxyphenyl)ethyl-O-\u03b1-rhamnopyrano syl-(1-4)-2-O-(E)caffeoyl-\u03b1-arabinopyranoside\u300134 6\u2032-O(\u987a\u5f0f-1\uff0c4-\u4e8c\u7f9f\u57fa\u73af\u5df1\u4e59\u9170\u57fa)-\u7c7b\u53f6\u5347\u9ebb\u82f7\uff3b6\u2032-O-(cis-1\uff0c4-dihydroxycyclohexanacetyl)- acteoside\uff3d\u300135\u6bdb\u82b1\u7403\u82b1\u82f7A(trichosanthoside A)\u300136\u9ec4\u82b1\u9a6c\u7f28\u4e39\u82f7B(fucatoside B)\u300137\u77f3\u8695\u82f7B(poliumoside B)\u300138\u7d2b\u830e\u5973\u8d1e\u82f7J(ligupurpuroside J)\u300139\u539a\u6734\u82f7F(magnoloside F)\u300140\u677e\u679c\u83ca\u82f7(echinacoside)\u300141\u5f02\u539a\u53f6\u8f66\u524d\u82f7(isocrassifolioside)\u300142(3\uff0c4)-3-\uff3b4-(beta-D-\u5421\u5583\u8461\u8404\u7cd6\u57fa\u6c27\u57fa)-3-\u7532\u6c27 \u57fa\u82ef\u57fa\uff3d\u7532\u57fa\u300143\u5bf9-\u9999\u8c46\u7d20-(6-O-\u5496\u5561\u9170\u57fa)-\u8461\u8404\u7cd6\u82f7\uff3bp- coumaroyl-(6-O-caffeoyl)-glucoside\uff3d\u300144 3\uff0c4-\u4e8c\u7f9f\u57fa\u82ef\u4e59\u9187\u82f7\u300145 2-\u7532\u6c27\u57fa-3\uff0c4\uff0c5-\u4e09\u7f9f\u57fa\u82ef\u4e59\u9187\u82f7\u300146 4-\uff3b2-(\u03b2-D-\u5421\u5583\u8461\u8404\u7cd6\u57fa\u6c27\u57fa)\u4e59\u57fa\uff3d-4-\u7f9f\u57fa-2\uff0c 5-\u73af\u5df1\u4e8c\u70ef\u300147(+)-8\u2032-hydroxyariciresinol
\n4\u2032-O-\u03b2-D-glucopyranoside\u300148\u5f02\u8fde\u7fd8\u82f7(Isoforsythiaside)\u3002<\/p>\n

1.3 \u6728\u8102\u7d20\u7c7b<\/p>\n

\u6728\u8102\u7d20\u7c7b\u662f\u8fde\u7fd8\u4e2d\u7684\u53c8\u4e00\u7c7b\u4e3b\u6210\u5206\uff0c\uff0c\u4ece\u8fde\u7fd8\u5c5e\u4e2d\u5206\u79bb\u5f97\u5230\u7684\u6728\u8102\u7d20\u7c7b\u6210\u5206\u591a\u4e3a\u53cc\u73af\u6c27\u6728\u8102\u7d20\u53ca\u6728\u8102\u5185\u916f\u3002\u53cc\u73af\u6c27\u6728\u8102\u7d20\u5305\u62ec\u8fde\u7fd8\u8102\u7d20\uff08\uff30\uff48\uff49\uff4c\uff4c\uff59\uff47\uff45\uff4e\uff49\uff4e\uff09\u3001\u8fde\u7fd8\u82f7\uff08\uff30\uff48\uff49\uff4c\uff4c\uff59\uff52\uff49\uff4e\uff09\u3001\u677e\u8102\u7d20\uff08\uff30\uff49\uff4e\uff4f\uff52\uff45\uff53\uff49\uff4e\uff4f\uff4c\uff09\u3001\u677e\u8102\u7d20-\u03b2-\uff24-\u8461\u8404\u7cd6\u82f7\uff08\uff30\uff49\uff4e\uff4f\uff52\uff45\uff53\uff49\uff4e\uff4f\uff4c\u03b2-\uff24-\uff47\uff4c\uff55\uff43\uff4f\uff53\uff49\uff44\uff45\uff09\u3001\u8868\u677e\u8102\u7d20\uff08\uff25\uff50\uff49\uff50\uff49\uff4e\uff4f\uff52\uff45\uff53\uff49\uff4e\uff4f\uff4c\uff09\u3001\u8868\u677e\u8102\u7d20-\uff14-\u03b2-\uff24-\u8461\u8404\u7cd6\u82f7\u7b49\u3002\u6728\u8102\u5185\u916f\u5305\u62ec\u7f57\u6c49\u677e\u8102\u7d20\uff08\uff2d\uff41\uff54\uff41\uff49\uff52\uff45\uff53\uff49\uff4e\uff4f\uff4c\uff09\u3001\u7f57\u6c49\u677e\u82f7\uff08\uff2d\uff41\uff54\uff41\uff49\uff52\uff45\uff53\uff49\uff4e\uff4f\uff53\uff49\uff44\uff45\uff09\u3001\u725b\u84a1\u5b50\u82f7\u5143\uff08\uff21\uff52\uff43\uff54\uff49\uff47\uff45\uff4e\uff49\uff4e\uff09\u548c\u725b\u84a1\u5b50\u82f7\uff08\uff21\uff52\uff43\uff54\uff49\uff49\uff4e\uff09\u7b49\u3002<\/p>\n

\u4e3b\u8981\u5305\u62ec\uff1a\u8fde\u7fd8\u82f7\u3001\u8fde\u7fd8\u8102\u7d20\u3001\u725b\u84a1\u5b50\u82f7\u3001\u725b\u84a1\u5b50\u82f7\u5143\u3001\u7f57\u6c49\u677e\u8102\u7d20\u3001\u7f57\u6c49\u677e\u82f7\u3001\u4e8c\u7532\u57fa\u7f57\u6c49\u677e\u8102\u7d20\u3001\uff08+\uff09-\u677e\u8102\u7d20\u3001\uff08+\uff09-\u8868\u677e\u8102\u7d20\u3001\u5f02\u6a44\u6984\u8102\u7d20\u3001\u5f02\u843d\u53f6\u677e\u8102\u7d20-4-O-\u03b2-D-\u8461\u8404\u7cd6\u82f7\u3001\uff08+\uff09-\u677e\u8102\u7d20-\u03b2-D-\u8461\u8404\u7cd6\u82f7\u3001\u5f02\u843d\u53f6\u677e\u8102\u7d20-9'-O-\u03b2-D-\u8461\u8404\u7cd6\u82f7\u3001\u677e\u8102\u7d20\u5355\u7532\u57fa\u919a-4-O-\u03b2-D-\u8461\u8404\u7cd6\u82f7\u30017'-epi-8-\u7f9f\u57fa\u677e\u8102\u7d20\u30018-\u7f9f\u57fa\u677e\u8102\u7d20\u3001\uff08+\uff09-inoresininol-4-Oglucoside\u3001\u843d\u53f6\u677e\u8102\u7d20\u3001\u5f02\u5e38\u53f6\u677e\u8102\u7d20\u3001\u88c2\u73af\u5f02\u843d\u53f6\u677e\u8102\u7d20\u3001\u677e\u8102\u7d20\u5355\u7532\u57fa\u919a-D-\u8461\u8404\u7cd6\u82f7\u3001\uff08+\uff09-\u8868\u677e\u8102\u7d20-4-\u03b2-D-\u8461\u8404\u7cd6\u82f7\u3001forsythialan
\nA\u3001forsythialanB\u3001calceolarioside B\u3001dimethylmatairesinol\u7b49\u3002<\/p>\n

\u53e6\u6709\u62a5\u9053\u542b\uff1a49\u677e\u8102\u915a\u300150
\n(+)-\u8fde\u7fd8\u82f7\uff3b(+)-phillyrin\uff3d\u300151 (+)-\u677e\u8102\u7d20\uff3b(+)-pinoresinol\uff3d\u300152 (+)-\u677e\u8102\u7d20-4-O-\u03b2-D-\u8461\u8404\u7cd6\u82f7\uff3b(+)-pinores inol-4-O-\u03b2-D-glucoside\uff3d\u300153 (+)-\u8868\u677e\u8102\u7d20-4-O-\u03b2-D-\u8461\u8404\u7cd6\u82f7\uff3b(+)-epipi noresinol-4-O-\u03b2-D-glucoside\uff3d\u300154 (+)-\u8868\u677e\u8102\u7d20-4\u2032-O-\u03b2-D-\u8461\u8404\u7cd6\u82f7\uff3b(+)-epipi noresinol-4\u2032-O-\u03b2-D-glucoside\uff3d\u300155
\n(+)-8-hydroxyepipinoresinol-4\u2032-O-\u03b2-D-glucopyr anoside\u300156 (+)-8-hydroxyepipinoresinol-4-O-\u03b2-D-glucopyr anoside\u300157 (+)-\u5f02\u843d\u53f6\u677e\u8102\u9187-9-O-\u03b2-D-\u8461\u8404\u7cd6\u82f7\uff3b(+)- isolariciresino-9-O-\u03b2-D-glucopyranoside\uff3d\u300158 \u6deb\u7f8a\u85ff\u82f7E4(icariside E4)\u300159 dihydrodehydrodiconiferyl
\nalcohol-4-O-\u03b2-D-glucoside\u300160 4-O-\u9f20\u674e\u7cd6\u57fa-7S\uff0c8R-7\u2032\uff0c8\u2032-\u8d64\u5f0f-\u849c\u82a5\u8304\u8102\u7d20(4- O-rhamnosyl-7S\uff0c8R-7\u2032\uff0c8\u2032-erythro-sisymbrifolin)\u300161 \u5f00\u73af\u5f02\u843d\u53f6\u677e\u6811\u8102\u915a\u300162 (+)-\u8fde\u7fd8\u8102\u7d20\uff3b(+)-phillygenin\uff3d\u300163 (-)-\u725b\u84a1\u5b50\u82f7\uff3b(-)-arctiin\uff3d\u300164 \u725b\u84a1\u5b50\u82f7\u5143\u300165 (-)-\u7f57\u6c49\u677e\u8102\u7d20\uff3b(-)-matairesinol\uff3d\u300166 (-)-\u7f57\u6c49\u677e\u916f\u82f7\uff3b(-)-matairesinoside\uff3d\u300167 (-)-\u4e8c\u7532\u57fa\u7f57\u6c49\u677e\u8102\u7d20\uff3b(-)-dimethylmatairesinol\uff3d\u300168( +)-\u8868\u677e\u8102\u7d20\uff3b(+)-epipinoresinol\uff3d\u300169 \u5f02\u6a44\u6984\u8102\u7d20(isoolivil)\u300170 \u5f02\u843d\u53f6\u677e\u8102\u7d20-4-O-\u03b2-D-\u8461\u8404\u7cd6\u82f7(isolariciresinol- 4-O-\u03b2-D-glucoside)\u300171 \u5f02\u843d\u53f6\u677e\u8102\u7d20-9\uff07-O-\u03b2-D-\u8461\u8404\u7cd6\u82f7\uff3b(+)-isolaricir esinol-9\uff07-O-\u03b2-D-glucopyranoside\uff3d\u300172 (+)-\u677e\u8102\u7d20\u5355\u7532\u57fa\u919a-4-O-\u03b2-D-\u8461\u8404\u7cd6\u82f7\uff3b(+)- pinoresinol monomethyl
\nether-4-O-\u03b2-D-glucoside\uff3d\u300173 7\u2032-epi-8-\u7f9f\u57fa\u677e\u8102\u7d20(7\u2032-epi-8-Hydroxypinoresinol)\u300174 8-\u7f9f\u57fa\u677e\u8102\u7d20(8-Hydroxypinoresinol)\u300175
\n(+)-inoresininol-4-O-glucoside\u300176 (+)-\u843d\u53f6\u677e\u8102\u7d20\uff3b(+)-lariciresinol\uff3d\u300177\u5f02\u843d\u53f6\u677e\u8102\u7d20\u300178 (-)-\u88c2\u73af\u5f02\u843d\u53f6\u677e\u8102\u7d20\uff3b(-)-secoisolariciresinol\uff3d\u300179 \u677e\u8102\u7d20\u5355\u7532\u57fa\u919a-D-\u8461\u8404\u7cd6\u82f7\u300180\u8fde\u7fd8\u5170A(forsythialan
\nA)\u300181 \u8fde\u7fd8\u5170B(forsythialan B)\u300182 calceolarioside B\u300183 dimethylmatairesinol\u300184 forsythiayanoside A\u300185 forsythiayanoside B\u300186 (+)-\u5f02\u843d\u53f6\u677e\u8102\u7d20-9-O-\u03b2-D-\u5421\u5583\u8461\u8404\u7cd6\u82f7 \uff3b(+)-isolariciresinol-9-O-\u03b2-D-glucopyranoside\uff3d\u300187 glochidioboside\u300188 cedrusin\u300189 (+)-\u4e01\u9999\u6811\u8102\u9187\uff3b(+)-syringaresinol\uff3d\u300190 (+)-1-\u7f9f\u57fa\u4e01\u9999\u6811\u8102\u9187\uff3b(+)-1-hydroxysyringares inol\uff3d\u300191 erythro-buddlenol E\u300192(+)-1-\u7f9f\u57fa-\u677e\u8102\u7d20-4\u2032-O-\u03b2-D-\u5421\u5583\u8461\u8404\u7cd6\u82f7 \uff3b(+)-1-hydroxypinoresinol-4\u2032\u2032-O-\u03b2-D-glucopyrano side\uff3d\u300193 (+)-\u5f02\u843d\u53f6\u677e\u8102\u7d20-6\u03b1-O-\u03b2-D-\u5421\u5583\u8461\u8404\u7cd6\u82f7 \uff3b(+)-isolariciresinol-6\u03b1-O-\u03b2-D-glucopyranoside\uff3d\u300194 (-)-\u6a44\u6984\u8102\u7d20-4\u2032-O-\u03b2-D-\u5421\u5583\u8461\u8404\u7cd6\u82f7\uff3b(-)-olivil-4\u2032-O-\u03b2-D-glucoside\uff3d\u300195 3\uff0c4-\u4e8c\u7f9f\u57fa-\u70ef\u4e19\u57fa\u82ef4-O-\u03b2-D-\u5421\u5583\u6728\u7cd6\u57fa-(1\u21926)-\u03b2-D-\u5421\u5583\u8461\u8404\u7cd6\u82f7\u300196 (+)-\u677e\u8102\u7d20\u5355\u7532\u57fa\u919a\uff3b(+)-pinoresinol
\nmonomethyl e ther\uff3d\u300197
\n5\u2032-\u7532\u6c27\u57fa\u843d\u53f6\u677e\u8102\u7d20\u300198
\nMedioresinol\u300199 \u8fde\u7fd8\u5170C(Forsythialan C)\u3001100 \u8fde\u7fd8\u5170D(Forsythialan D)\u3001101(+)-1-\u7f9f\u57fa-\u677e\u8102\u7d20-4\u2032-O-\u03b2-D-\u5421\u5583\u8461\u8404\u7cd6\u82f7\uff3b(+)-1-hydroxypinoresinol-4\u2032-O-\u03b2-D-glucopyrano side\uff3d\u3001102(-)-\u725b\u84a1\u5b50\u7d20\uff3b(-)-arctigenin\uff3d\u3001103 (+)-\u5f02\u843d\u53f6\u677e\u8102\u9187\uff3b(+)-isolariciresinol\uff3d\u3001104(-)-\u6a44\u6984\u8102\u7d20\uff3b(-)-olivil\uff3d\u3001105\u53cc\u73af\u6c27\u8fde\u7fd8\u5185\u916f\u3001106 forsythialanside E\u3001107(+)-lariciresinol-4-O-\u03b2-D-glucopyranoside\u3001108 rel-(7R\uff0c8'R\uff0c8S)-forsythialan C\u3001109 rel-(7R\uff0c8'R\uff0c8R)-forsythialan C\u3001110 4-O-demethylforsythenin\u3001111 6''-Acetyl forsythin\u3001112\u8868\u677e\u8102\u915a\u3002<\/p>\n

2009 \u5e74\u51af\u536b\u751f\u7b49\u53c8\u9996\u6b21\u4ece\u8fde\u7fd8\u4e2d\u5206\u5f97\u5f02\u843d\u53f6\u677e\u8102\u7d20-4-O-\u03b2-D-\u8461\u8404\u7cd6\u82f7\u3001Salicifoliol\u3001\u5f02\u6a44\u6984\u8102\u7d20\u3001\u5f02\u843d\u53f6\u677e\u8102\u7d20-9\u2032-O-\u03b2-D-\u8461\u8404\u7cd6\u82f7\u3002<\/p>\n

1.4 \u4e09\u841c\u7c7b<\/p>\n

\u8fde\u7fd8\u4e2d\u7684\u4e09\u841c\u7c7b\u6210\u5206\u4e3b\u8981\u6709\u767d\u6866\u8102\u9178\u3001\u9f50\u58a9\u679c\u9178\u3001\u718a\u679c\u9178\u7b49\u30022001 \u5e74Shamsur ASR \u7b49\u4ece\u4e2d\u5206\u5f972 \u4e2a\u65b0\u4e09\u841c\u7c7b\u7269\u8d28\uff1a3\u03b2-\u4e59\u9170\u57fa-20\uff0c25-\u73af\u6c27\u8fbe\u739b\u70f7-24\u03b1-\u9187\u548c3\u03b2-\u4e59\u9170\u57fa-20\uff0c25-\u73af\u6c27\u8fbe\u739b\u70f7-24\u03b2-\u9187\u30022008 \u5e74\u65b9\u9896\u7b49\u4ece\u8fde\u7fd8\u4e2d\u5206\u5f972\u03b1-\u7f9f\u57fa\u767d\u6866\u8102\u9178\u30012\u03b1\uff0c23-\u7f9f\u57fa\u718a\u679c\u9178\u30022009 \u5e74\u624d\u8c26\u7b49\u53c8\u4ece\u8fde\u7fd8\u7c7d\u4e2d\u9996\u6b21\u5206\u5f97\u03b2-\u9999\u6811\u8102\u9187\u4e59\u9178\u916f\u300120S-\u8fbe\u739b\u70f7-24-\u70ef-3\u03b2\uff0c20-\u4e8c\u9187-3-\u4e59\u9178\u916f\u548c20S\uff0c24S-\u73af\u6c27\u8fbe\u739b\u70f7-25-\u9187-3\u03b1-\u7f9f\u57fa\u4e59\u9178\u916f\u3002<\/p>\n

\u53e6\u6709\u62a5\u9053\u542b\uff1a114\u5b89\u535a\u7acb\u9178\u3001115\u8fbe\u739b-24-\u70ef-3\u03b2-\u4e59\u9170\u6c27\u57fa-20S-\u9187\u3001116 3\u03b2-\u4e59\u9170\u57fa-20S-\u8fbe\u739b-23-\u70ef-20\uff0c25-\u4e8c\u9187\u3001117\u9f50\u58a9\u679c\u9178(oleanolic acid)\u3001118\u718a\u679c\u9178(ursolic acid)\u3001119\u8def\u8def\u901a\u9178\u3001120\u79d1\u7f57\u7d22\u9178\u3001121 Myricadiol\u3001122 2\u03b2-\u7f9f\u57fa\u718a\u679c\u9178\u3001123\u767d\u6866\u8102\u9178(betulinic acid)\u3001124\u5f02\u964d\u9999\u841c\u70ef\u9187\u4e59\u9178\u916f(iso-baucerenyl acetate)\u3001125\u03b2-\u9999\u6811\u8102\u9187\u4e59\u9178\u916f(\u03b2-amyrin acetate)\u3001126 3\u03b2-\u4e59\u9178\u57fa-20\uff0c25-\u73af\u6c27\u8fbe\u739b\u70f7-24\u03b1-\u9187(3\u03b2-acetyl-20\uff0c25-epoxydammarane-24\u03b1-ol)\u3001127
\n3\u03b2-\u4e59\u9178\u57fa-20\uff0c25-\u73af\u6c27\u8fbe\u739b\u70f7-24\u03b2-\u9187(3\u03b2-acetyl-20\uff0c25-epoxydammarane-24\u03b2-ol)\u3001128
\n3\u03b2-\u4e59\u9178\u57fa-20\uff0c25-\u73af\u6c27\u8fbe\u739b\u70f7-24-\u9187(3\u03b2-acetyl-20\uff0c25-epoxydammarane-24-ol)\u3001129 20(S)-\u8fbe\u739b\u70f7-24-\u70ef-3\u03b2\u3001130 20-\u4e8c\u9187-3-\u4e59\u9178\u916f\u3001131\u4e59\u9170\u9f50\u58a9\u679c\u9178\u3001132\u5546\u9646\u79cd\u9178\u3001133\u79ef\u96ea\u8349\u9178\u3001134\u82ef\u7532\u9187\u6a31\u8349\u7cd6\u82f7\u3001135\u82ef\u7532\u9187-O-(2\uff07-O-\u03b2-D-\u5421\u5583\u6728\u7cd6\u82f7)-\u03b2-D-\u5421\u5583\u8461\u8404\u7cd6\u82f7\u3001136\u7532\u57fa-\u03b2-D-\u5421\u5583\u8461\u8404\u7cd6\u3001137\u4e94\u798f\u82b1\u82f7\u9178(adoxosidic acid)\u3001138 2\u03b1\uff0c23-\u7f9f\u57fa\u718a\u679c\u9178\u3001139\u957f\u7ba1\u5927\u9752\u7d20A\u3001140 20S\uff0c24S-\u73af\u6c27\u8fbe\u739b\u70f7-25-\u9187-3\u03b1-\u7f9f\u57fa\u4e59\u9178\u916f\u3001141 19-hydroxylabda-8(17)\uff0c13(Z)-dien-15-oic acid\u3001142 18-hydroxylabda-8(17)\uff0c13(E)-dien-15-oic acid\u3001143 19-formyllabda-8(17)\uff0c13(E)-dien-15-oic acid\u3001144 19-formyllabda-8(17)\uff0c13(Z)-dien-15-oic acid\u3001145 labda-8(17)\uff0c13(Z)-dien-15\uff0c18-dioic acid\u3001146 18-hydroxy-7-oxolabda-8(9)\uff0c13(E)-dien-15-oic acid\u3001147 17\uff0c19-dihydroxylabda-7(8)\uff0c13(E)-dien-15-oic acid\u3001148 forsythidin A\u3001149 adoxosidic acid
\n10-p-hydroxyphenlacetate\u3001150 3\u03b2-acetoxy-25-methoxydammar-23-en-20\u03b2-ol\u3001151 methyl-3\u03b1-hydroxyolean-18-ene-28-oate\u3001152 3\u03b1\uff0c18-dihydroxy-ent-labda-8(17)\uff0c13E-dien-15-oic acid\u3001153 3\u03b1-hydroxy-ent-labda-8(17)\uff0c12E-14-trien-18-ol\u3001154 3\u03b1-hydroxy-ent-labda-8(17)\uff0c12E-14-trien-19-oic acid\u3002<\/p>\n

1.5 \u915a\u9178\u7c7b<\/p>\n

2005 \u5e74\u8d75\u6587\u534e\u7b49\u4ece\u8fde\u7fd8\u4e2d\u5206\u5f97\u5496\u5561\u9178\u30022009 \u5e74\u51af\u536b\u751f\u7b49\u4ece\u4e2d\u5206\u5f97\u5496\u5561\u9178\u7532\u916f\u548c\u5bf9\u7f9f\u57fa\u82ef\u4e59\u9178\u7532\u916f\u3002\u540c\u5e74\u624d\u8c26\u7b49\u53c8\u4ece\u4e2d\u5206\u5f97\u539f\u513f\u8336\u9178\u3002<\/p>\n

\u4e3b\u8981\u542b\uff1a245\u786c\u8102\u9178(stearic
\nacid)\u3001246\u68d5\u6988\u9178\u3001247\u9999\u835a\u5170\u9178\u3001248\u7425\u73c0\u9178\u3001249\u4e01\u4e8c\u9178(succinicacid)\u3001250 4-\u7f9f\u57fa-4-\u5f02\u4e19\u57fa-1-\u73af\u5df1\u70ef\u7532\u9178\u3001251 oleuropeic acid\u3001252(2\uff32\uff0c3S)-3-hydroxy-2-hydroxypropylpyrrolidine\u3001253\u594e\u5b81\u9178\u3001254 4-\u7f9f\u57fa\u8089\u6842\u9178\u3001255\u963f\u9b4f\u9178(ferulic acid)\u3001256\u03b2-\u8c37\u753e\u9178\u3001257 3\uff0c4-\u4e8c\u7f9f\u57fa\u82ef\u7532\u9178\u3001258 3-acetoxy-20-hydroxyursan-28-oicacid\u3001259 agatholic acid\u3001260 3-hydroxyanticopalic acid\u3001261\u4e01\u9999\u9178(Syringic acid)\u3001262\u6ca1\u98df\u5b50\u9178(Gallic)\u3001263\u539f\u513f\u8336\u919b(Protocatechuic aldehyde)\u3001264\u9999\u8349\u9178(Vanillic acid)\u3001265\u5bf9\u7f9f\u57fa\u82ef\u7532\u9178(p-hydroxybenzoic acid)\u3001266\u539f\u513f\u8336\u9178(Protocatechuic acid)\u3001267\u5bf9\u7f9f\u57fa\u82ef\u7532\u9187(p-hydroxybenzyl alcohol)\u3001268 1-(4-\u7f9f\u57fa\u82ef\u57fa)-2\uff0c3-\u4e8c\u7f9f\u57fa\u4e19\u916e\uff3b1-(4-hydroxy-phenyl)-2\uff0c3-dihydroxyacetone\uff3d\u3001269\u5355\u5b81\u9178(Tannic acid)\u3001270\u5bf9\u7f9f\u57fa\u82ef\u4e59\u9178(Methylp-hydroxyphenylacetate)\u3001271\u5bf9\u7f9f\u57fa\u82ef\u57fa\u4e59\u9178\u7532\u916f(p-hydroxyphenylacetic
\nacidmethylest-er)\u3001272\u8fde\u7fd8\u9187\u916f(\uff32engyolester)\u3001273 4-(2-\u7f9f\u57fa\u4e59\u57fa)\u82ef\u7532\u919b\uff3b4-(2-hydroxyethyl)benzalde-hyde\uff3d\u3001274\u5bf9\u7532\u6c27\u57fa\u82ef\u4e59\u919b(Methoxyphenylacetaldehyde)\u3001275 Forsythiayarosiade C\u3001276 Forsythiayarosiade D\u3001277\u5bf9\u7f9f\u57fa\u82ef\u4e59\u9187(p-hydroxyphenylethanol)\u3001278 3\uff0c4-\u4e8c\u7f9f\u57fa\u82ef\u4e59\u9187(3\uff0c4-dihydroxyphenylethanol)\u3001279
\n4-hydroxyphenethyl-2-(4-hydroxyphenl)acetate\u3001280\u5496\u5561\u9178(caffeic acid)\u3001281\u53cd\u5f0f\u9999\u8c46\u9178(trans-coumaric
\nacid)\u3001282\u53cd\u5f0f\u963f\u9b4f\u9178(transferulic acid)\u3001283\u53cd\u5f0f\u5496\u5561\u9178\u7532\u916f\uff3b(E)-caffeic acid
\nmethylester\uff3d\u3001284\u7eff\u539f\u9178(chlorogenic acid)\u3001285\u4e39\u53c2\u7d20\u7532\u916f\u3001286\u5bf9\u9999\u8c46\u9178\u3001287 1\uff0c2\uff0c4-\u82ef\u4e09\u915a\u3001288\u5bf9\u7f9f\u57fa\u82ef\u7532\u919b\u3001289\u5f02\u9999\u8349\u9178\u3001290\u5bf9\u7f9f\u57fa\u82ef\u7532\u9178\u7532\u916f\u3001291 4-hydroxyphenethyl
\n2(4-hydroxyphenyl)acetate\u3001292\u5bf9\u7f9f\u57fa\u82ef\u4e59\u9178\u4e59\u916f\u3001293 suspenolic acid\u3002<\/p>\n

1.6\u9ec4\u916e\u7c7b<\/p>\n

\u76ee\u524d\uff0c\u4ece\u8fde\u7fd8\u5c5e\u4e2d\u5206\u79bb\u5f97\u5230\u7684\u9ec4\u916e\u7c7b\u5316\u5408\u7269\u4e3b\u8981\u6709\u82a6\u4e01\uff08\uff52\uff55\uff54\uff49\uff4e\uff09\u3001\u69f2\u76ae\u7d20\uff08\uff51\uff55\uff45\uff52\uff43\uff45\uff54\uff49\uff4e\uff09\u3001\u5f02\u69f2\u76ae\u7d20\uff08\uff49\uff53\uff4f\uff51\uff55\uff45\uff52\uff43\uff45\uff54\uff49\uff4e\uff09\u3001\u7d2b\u4e91\u82f1\u82f7\uff08\uff41\uff53\uff54\uff52\uff41\uff47\uff41\uff4c\uff49\uff4e\uff09\u3002<\/p>\n

\u53e6\u6709\u62a5\u9053\u542b\uff1a215\u69f2\u76ae\u7d20(quercetin)\u3001216\u5f02\u69f2\u76ae\u7d20(isoquercetin)\u3001217\u82a6\u4e01(rutin)\u3001218\u7d2b\u4e91\u82f1\u82f7(astragalin)\u3001219\u6c49\u9ec4\u82a9\u7d20-7-O-\u03b2-D-\u8461\u8404\u7cd6\u82f7(wogonin-7-O-\u03b2-D-glucoside)\u3001220\u9ec4\u745e\u70f7\u916e(rengyolone)\u3001221\u5343\u91cc\u5149\u5185\u916f\u3001222 cleroindicin C\u3001223\u9ec4\u82a9\u82f7(baicalin)\u3001224\u9e22\u5c3e\u82f7\u3001225\u7a57\u82b1\u6749\u53cc\u9ec4\u916e\u3001226\u5f02\u69f2\u76ae\u82f7\u3001227\u6a59\u76ae\u82f7(hesperidin)\u3001228\u9ec4\u82a9\u7d20\u3001229\u6c49\u9ec4\u82a9\u7d20\u3001230\u91d1\u4e1d\u6843\u82f7(hyperin)\u3001231 3'\uff0c6-\u4e8c\u7f9f\u57fa\u69f2\u76ae\u7d20\u3001232 6-\u7f9f\u57fa\u4e8c\u6c22\u9ec4\u916e\u3001233\u4e8c\u6c22\u6768\u6885\u7d20\u3001234\u82b8\u9999\u53f6\u82f7\uff3b22\uff3d 235\u7ffb\u767d\u53f6\u82f7A(potengriffioside A)\u3001236\u6728\u7280\u8349\u82f7(galuteolin)\u3001237\u5c71\u67f0\u915a(kaempferol)\u3001238\u5f02\u9f20\u674e\u7d20(isorhamnetin)\u3001239\u6728\u7280\u8349\u7d20(lunteolin)\u3001240\u5f02\u9f20\u674e\u7d20-3-O-\u03b1-L-\u5421\u5583\u9f20\u674e\u7cd6\u57fa-(1\u21922)-\u03b2-D-\u5421\u5583\u8461\u8404\u7cd6\u82f7\uff3bisorhamnetin-3-O-\u03b1-L-rhamnopyr anosyl-(1\u21922)-\u03b2-D-glucopyranoside\uff3d\u3001241\u7f9f\u57fa\u718a\u679c\u9178(Hydroxyursolic acid)\u3001242\u9f20\u674e\u7d20(Isorhamnetin)\u3001243 Luteolin-7-O-(6-Omalonyl-\u03b2-D-glucoside)\u3001244\u91d1\u5408\u6b22\u7d20(Acacetin)\u3002<\/p>\n

\u5218\u4f1a\u8d85\u7b49\u4ece\u8fde\u7fd8\u82b1\u74e3\u4e2d\u5f97\u5230\u8fde\u7fd8\u82b1\u8272\u82f7\u3002\u738b\u71d5\u7b49\u62a5\u9053\u4e86\u5728\u4e0d\u540c\u91c7\u6458\u65f6\u671f\u8fde\u7fd8\u53f6\u7684\u603b\u9ec4\u916e\u542b\u91cf\uff0c\uff13\u6708\u91c7\u6458\u7684\u521a\u840c\u53d1\u5e7c\u53f6\u4e2d\u603b\u9ec4\u916e\u542b\u91cf\u6700\u9ad8\u3002<\/p>\n

1.7 \u5176\u4ed6<\/p>\n

\u76ee\u524d\u4e3b\u8981\u6709\uff1aforsythol(\u8fde\u7fd8\u915a)\u3001\u786c\u8102\u9178\u3001\u68d5\u6988\u9178\u3001\u03b2-\u8c37\u753e\u9187\u3001\u7425\u73c0\u9178\u3001\u4e01\u4e8c\u9178(succinicacid)\u30012\uff0c3-\u4e8c\u7f9f\u7532\u57fa-4-(3 \uff0c4
\n-\u4e8c\u7532\u6c27\u57fa\u82ef\u57fa)-\u03b3-\u4e01\u5185\u916f\u3001\u4e01\u56db\u9187(erythrito1)\uff1b\u5fae\u91cf\u5143\u7d20\uff0c\u5982\u94dc(Cu)\u3001\u94b4(Co)\u3001\u954d(Ni)\u3001\u950c(Zn)\u3001\u94c1(Fe)\u3001\u9530(Mn)\u3001\u94c5(Pb)\u3001\u7837(As)\u53ca\u795e\u7ecf\u9170\u80fa\u7c7b\u5316\u5408\u7269\u3001\u725b\u84a1\u82f7\u5143\u7b49\u3002<\/p>\n

2005 \u5e74\u8d75\u6587\u534e\u7b49\u4ece\u8fde\u7fd8\u75c5\u6bd2\u6e05\u80f6\u56ca\u4e2d\u5206\u5f97\u9ec4\u916e\u7c7b\u5316\u5408\u7269\u82a6\u4e01\u3002<\/p>\n

2009 \u5e74\u624d\u8c26\u7b49\u4ece\u8fde\u7fd8\u7c7d\u4e2d\u5206\u5f97\u4e00\u79cd\u65b0\u5929\u7136\u4ea7\u7269\u516b\u6c22-1H\uff0c5H-\u4e8c\u5421\u54af[1\uff0c2-a\uff1a1\u2032\uff0c2\u2032- d ]\u5421\u55ea\u3002\u540c\u5e74\u51af\u536b\u751f\u7b49\u5206\u5f97L-\u9f20\u674e\u7cd6\u3002<\/p>\n

2. \u836f\u7406\u4f5c\u7528<\/p>\n

\u8fde\u7fd8\u7684\u836f\u7406\u6d3b\u6027\u4e3b\u8981\u8868\u73b0\u4e3a\u6297\u6c27\u5316\u6d3b\u6027\u3001\u6297\u83cc\u6d3b\u6027\u3001\u6297\u708e\u6d3b\u6027\u3001\u6297\u75c5\u6bd2\u6d3b\u6027\u3001\u6297\u80bf\u7624\u6d3b\u6027\u4ee5\u53ca\u89e3\u70ed\u9547\u75db\u6d3b\u6027\u7b49\u3002\u8fd15 \u5e74\u6765\u7814\u7a76\u6700\u591a\u7684\u662f\u8fde\u7fd8\u7684\u6297\u6c27\u5316\u6d3b\u6027\uff0c\u53e6\u5916\u8fd8\u53d1\u73b0\u8fde\u7fd8\u6709\u6297\u8fc7\u654f\u6d3b\u6027\u3002<\/p>\n

2.1 \u6297\u6c27\u5316\u6d3b\u6027<\/p>\n

\u79d1\u5b66\u7814\u7a76\u8868\u660e\uff0c\u4eba\u4f53\u8870\u8001\u7684\u539f\u56e0\u4e0e\u4eba\u4f53\u5185\u81ea\u7531\u57fa\u592a\u591a\uff0c\u5f15\u8d77\u6c27\u5316\u592a\u8fc7\uff0c\u6d88\u8017\u592a\u591a\u80fd\u91cf\uff0c\u7834\u574f\u6b63\u5e38\u7ec6\u80de\u6709\u5173\u3002\u9632\u6cbb\u8870\u8001\u4e0e\u5982\u4f55\u9632\u6cbb\u81ea\u7531\u57fa\u6c27\u5316\u606f\u606f\u76f8\u5173\u3002\u8fde\u7fd8\u82b1\u9187\u63d0\u7269\u53ef\u4ee5\u901a\u8fc7\u6e05\u9664\u6d3b\u6027\u6c27\u81ea\u7531\u57fa\u548c\u63d0\u9ad8\u6297\u6c27\u5316\u9176\u6d3b\u529b\u4fdd\u62a4\u7ebf\u7c92\u4f53\uff0c\u8fde\u7fd8\u53f6\u4e2d\u591a\u915a\u7c7b\u7269\u8d28\u53ef\u4ee5\u6e05\u9664\u4e9a\u785d\u9178\u76d0\u548c\u963b\u65ad\u4e9a\u785d\u80fa\u7684\u5f62\u6210\u3002<\/p>\n

\u8fd1\u5e74\u6765\u4eba\u4eec\u5df2\u5c06\u8fde\u7fd8\u63d0\u53d6\u7269\u4f5c\u4e3a\u4e00\u79cd\u6f5c\u5728\u7684\u6297\u6c27\u5316\u5242\uff0c\u76ee\u524d\u7684\u7814\u7a76\u4e3b\u8981\u96c6\u4e2d\u5728\u5bfb\u627e\u5176\u7406\u60f3\u7684\u6297\u6c27\u5316\u90e8\u4f4d\u4e0a\u3002\u8fde\u7fd8\u679c\u5b9e\u548c\u8fde\u7fd8\u53f6\u63d0\u53d6\u7269\u4e2d\u7684\u4e59\u9178\u4e59\u916f\u3001\u6b63\u4e01\u9187\u548c\u4e59\u9187\u63d0\u53d6\u90e8\u4f4d\u5747\u6709\u8f83\u5f3a\u7684\u6297\u6c27\u5316\u6d3b\u6027\u3002<\/p>\n

\u915a\u9178\u7c7b\u6210\u5206\u662f\u8fde\u7fd8\u53d1\u6325\u6297\u6c27\u5316\u4f5c\u7528\u7684\u4e3b\u8981\u6d3b\u6027\u7269\u8d28\u3002<\/p>\n

HUANG C K\u7b49\u901a\u8fc7\u7814\u7a76\u53d1\u73b0\u8fde\u7fd8\u916f\u82f7\u53ef\u6291\u5236H2O 2 \u8bf1\u5bfc\u7684\u7ebf\u7c92\u4f53\u4f9d\u8d56\u6027\u51cb\u4ea1\u3002\u6b64\u5916\uff0c\u5728\u8fde\u7fd8\u916f\u82f7\u5904\u7406\u7684\u7ec6\u80de\u4e2d\u68c0\u6d4b\u5230Nrf2\u7684\u6838\u6c34\u5e73\u5347\u9ad8\u548c\u6297\u6c27\u5316\u9176(Mn\/SOD\u548cCAT)\u7684\u4e0a\u8c03\uff0c\u63d0\u793a\u8fde\u7fd8\u82f7\u7684\u6297\u6c27\u5316\u4f5c\u7528\u53ef\u80fd\u4e0eNrf2\u9014\u5f84\u7684\u6fc0\u6d3b\u6709\u5173\uff0c\u5e76\u4e14\u8fde\u7fd8\u916f\u82f7\u8fd8\u80fd\u6709\u6548\u963b\u6b62LPS\u8bf1\u5bfc\u7684\u7ec6\u80de\u6b7b\u4ea1\u548c\uff32OS\u7684\u4ea7\u751f\u3002\u56e0\u6b64\uff0c\u8fde\u7fd8\u916f\u82f7\u80fd\u6291\u5236\u8fc7\u6c27\u5316\u6c22\u8bf1\u5bfc\u7684\u6c27\u5316\u5e94\u6fc0\uff0c\u4e4b\u540e\u6fc0\u6d3b\u7ec6\u80de\u51cb\u4ea1\u4ece\u800c\u8d77\u5230\u6297\u6c27\u5316\u7684\u4f5c\u7528\u3002<\/p>\n

\u7a0b\u542f\u658c\u7b49\u91c7\u7528DPPH\u6e05\u9664\u6cd5\u6765\u8003\u5bdf\u8fde\u7fd8\u7684\u6297\u6c27\u5316\u6d3b\u6027\uff0c\u7814\u7a76\u7ed3\u679c\u8868\u660e\uff0c\u8fde\u7fd8\u7684\u4e0d\u540c\u90e8\u4f4d\u5bf9DPPH\u5747\u5177\u6709\u4e00\u5b9a\u7684\u6e05\u9664\u4f5c\u7528\uff0c\u8868\u73b0\u51fa\u6297\u6c27\u5316\u6d3b\u6027\u3002\u5176\u4e2d\uff0c\u8fde\u7fd8\u82b1\u53ca\u53f6\u5b50\u7684\u6297\u6c27\u5316\u80fd\u529b\u5747\u9ad8\u4e8e\u8fde\u7fd8\u679c\u5b9e\uff0c\u8868\u660e\u8fde\u7fd8\u4e2d\u603b\u915a\u7c7b\u5316\u5408\u7269\u6709\u4e00\u5b9a\u7684\u81ea\u7531\u57fa\u6e05\u9664\u4f5c\u7528\u3002<\/p>\n

2009 \u5e74\u674e\u5174\u6cf0\u7b49\u901a\u8fc7\u8fde\u7fd8\u82b1\u9187\u63d0\u7269\u53ca\u6c34\u63d0\u53d6\u7269\u5206\u522b\u5bf9\u5c0f\u9f20\u8fdb\u884c\u5b9e\u9a8c\u53d1\u73b0\uff0c\u8fde\u7fd8\u82b1\u9187\u63d0\u7269\u548c\u6c34\u63d0\u53d6\u7269\u5747\u80fd\u901a\u8fc7\u6e05\u9664\u6d3b\u6027\u6c27\u81ea\u7531\u57fa\u53ca\u63d0\u9ad8\u6297\u6c27\u5316\u9176\u6d3b\u529b\u6765\u4fdd\u62a4\u7ebf\u7c92\u4f53\uff0c\u5177\u6709\u4e00\u5b9a\u7684\u6297\u6c27\u5316\u53ca\u6297\u8870\u8001\u7684\u836f\u7528\u4ef7\u503c\u3002<\/p>\n

2008\u5e74\u66f2\u6b22\u6b22\u53d1\u73b0\u5f02\u8fde\u7fd8\u916f\u82f7\u3001\u8fde\u7fd8\u916f\u82f7\u548c\u8fde\u7fd8\u82f7\u8fd93 \u79cd\u5316\u5b66\u6210\u5206\u5bf9DPPH\u81ea\u7531\u57fa\u5747\u6709\u4e00\u5b9a\u7684\u6e05\u9664\u4f5c\u7528\uff0c\u5177\u6709\u6297\u6c27\u5316\u80fd\u529b\uff0c\u4e14\u524d2 \u79cd\u7269\u8d28\u7684\u6297\u6c27\u5316\u80fd\u529b\u5927\u5927\u4f18\u4e8e\u8fde\u7fd8\u82f7\uff0c\u662f\u65b0\u7684\u4f18\u79c0\u7684\u5929\u7136\u6297\u6c27\u5316\u5242\uff0c\u5e76\u7ed3\u5408HPLC\u542b\u91cf\u6d4b\u5b9a\u7ed3\u679c\u7c97\u7565\u4f30\u7b97\u51fa\u8fde\u7fd8\u6709\u8d85\u8fc750\uff05\u6297\u6c27\u5316\u80fd\u529b\u6765\u81ea\u4e8e\u8fde\u7fd8\u916f\u82f7\u3002<\/p>\n

2009 \u5e74Piao XL \u7b49\u7814\u7a76\u4e86\u8fde\u7fd8\u5bf9\u8fc7\u6c27\u785d\u57fa\u5f15\u8d77\u7684\u7ec6\u80de\u635f\u5bb3\u7684\u62b5\u6297\u4f5c\u7528\uff0c\u5e76\u53d1\u73b0\u53d1\u6325\u8fd9\u79cd\u6d3b\u6027\u7684\u6210\u5206\u4e3b\u8981\u662f\u8fde\u7fd8\u82f7\u548c8-\u7f9f\u57fa\u677e\u8102\u7d20\u30022010\u5e74\u8be5\u7814\u7a76\u7ec4\u5bf9\u96c4\u6027\u5927\u767d\u9f20\u7531\u654c\u8349\u5feb\u5f15\u8d77\u7684\u6c27\u5316\u538b\u529b\u7684\u4f53\u5916\u7814\u7a76\u53d1\u73b0\uff1a\u8fde\u7fd8\u4e2d\u7684\u8fde\u7fd8\u916f\u82f7A\u3001forsythialan
\nA\u548c\u8fde\u7fd8\u8102\u7d20\u53ef\u80fd\u662f\u8fde\u7fd8\u4e2d\u4e3b\u8981\u7684\u6297\u6c27\u5316\u6210\u5206\uff1b\u4e0d\u540c\u5242\u91cf\u7684\u8fde\u7fd8\u63d0\u53d6\u7269\uff08FSC\uff0c25\u300150 \u548c100 mg\u00b7kg-1\uff09\u9884\u5904\u7406\u8fc7\u7684\u8001\u9f20\uff08\u5e76\u7528\u7ef4\u751f\u7d20C\uff08100 mg\u00b7kg-1\uff09\u4f5c\u4e3a\u9633\u6027\u5bf9\u7167\uff09\uff0c15 d \u540e\u4e0e\u9634\u6027\u5bf9\u7167\u7ec4\u76f8\u6bd4\u8840\u6d46\u4e2d\u7684\u80bf\u7624\u574f\u6b7b\u56e0\u5b50-a\uff08TNF-a\uff09\u3001\u767d\u4ecb\u7d20-1b\uff08IL-1b\uff09\u548c\u767d\u4ecb\u7d20-6\uff08IL-6\uff09\u90fd\u6709\u660e\u663e\u964d\u4f4e\uff0c\u800c\u4e14FSC\u80fd\u663e\u8457\u589e\u5f3a\u8fc7\u6c27\u5316\u7269\u5c90\u5316\u9176\uff08SOD\uff09\u3001\u8c37\u80f1\u7518\u80bd\u8fc7\u6c27\u5316\u7269\u9176\uff08GSH-Px\uff09\u7684\u6d3b\u6027\uff0c\u63d0\u9ad8\u8c37\u80f1\u7518\u80bd\uff08GSH\uff09\u5728\u8840\u6d46\u3001\u809d\u810f\u548c\u80be\u810f\u7684\u542b\u91cf\uff0c\u540c\u65f6\u964d\u4f4e\u4e19\u4e8c\u919b\uff08MDA\uff09\u5728\u8840\u6d46\u548c\u80be\u810f\u7684\u542b\u91cf\u3002\u5e76\u4e14FSC\u7684\u6d3b\u6027\u8981\u4f18\u4e8e\u7ef4\u751f\u7d20C\u3002<\/p>\n

\u5218\u5efa\u6d32\u7b49\u901a\u8fc7\u590d\u5236\u8102\u591a\u7cd6LPS\u8bf1\u5bfc\u7684BEAS-2B\u708e\u75c7\u6a21\u578b\uff0c\u7136\u540e\u5bf9BEAS-2B \u7ec6\u80de\u6d3b\u529b\u91c7\u7528MTT \u6cd5\u8fdb\u884c\u68c0\u6d4b\uff0c\u7b5b\u9009\u51fa\u8fde\u7fd8\u4e59\u9187\u63d0\u53d6\u7269\uff08FSEE\uff09\u3001\u8fde\u7fd8\u916f\u82f7A\uff0860% FTA\uff09\u300190%\u8fde\u7fd8\u916f\u82f7A\uff0890%FTA\uff09\u6700\u597d\u7684\u7ed9\u836f\u6d53\u5ea6\uff1b\u914d\u5408Griess Reagent \u6cd5\u68c0\u6d4b\u7ec6\u80de\u57f9\u517b\u4e0a\u6e05\u6db2\u4e2d\u4e00\u6c27\u5316\u6c2e\u7684\u542b\u91cf\uff1b\u540c\u65f6\u9009\u7528\u5012\u7f6e\u663e\u5fae\u955c\u6765\u89c2\u6d4b\u7ec6\u80de\u5728\u53d7\u5230\u8fde\u7fd8\u63d0\u53d6\u7269\u4f5c\u7528\u540e\u8fde\u7684\u7ec6\u80de\u5f62\u6001\u53d8\u5316\uff1b\u5e76\u7528\u6d41\u5f0f\u7ec6\u80de\u672f\u68c0\u6d4b\u7ec6\u80de\u5185\u6d3b\u6027\u6c27\uff08ROS\uff09\u7684\u6c34\u5e73\uff1b\u7ed3\u679c\u53d1\u73b0\u8fde\u7fd8\u63d0\u53d6\u7269\u6297\u708e\u4f5c\u7528\u7684\u4e3b\u8981\u6d3b\u6027\u90e8\u4f4d\u4e3a90%FTA \u5176\u6297\u708e\u6d3b\u6027\u6210\u5206\u53ef\u80fd\u4e3a\u8fde\u7fd8\u916f\u82f7A\u3002<\/p>\n

\u7a0b\u5e7f\u4e1c\u901a\u8fc7\u91c7\u7528LPS\u8bf1\u5bfc\u9e21\u548c\u9e21\u813e\u6dcb\u5df4\u7ec6\u80de\u708e\u75c7\u6a21\u578b\uff0c\u53d1\u73b0\u5728\u53d1\u751f\u9e21\u813e\u6dcb\u5df4\u7ec6\u80de\u3001\u813e\u810f\u548c\u8840\u6db2\u4e2d\u7684\u708e\u75c7\u53d7\u5230\u8fde\u7fd8\u916f\u82f7A \u5f88\u597d\u7684\u6291\u5236\u4f5c\u7528\uff0c\u8fde\u7fd8\u916f\u82f7A \u8fd8\u80fd\u5f71\u54cd\u813e\u810f\u7684\u514d\u75ab\u529f\u80fd\uff0c\u4e3b\u8981\u4f53\u73b0\u5728\u8840\u6db2\u548c\u813e\u810f\u4e2d\u708e\u75c7\u56e0\u5b50\u7684mRNA \u548c\u542b\u91cf\u4e0b\u964d\uff0c\u6297\u6c27\u5316\u9176\u6d3b\u529b\u5728\u673a\u4f53\u4e2d\u589e\u5f3a\uff0c\u673a\u4f53\u81ea\u7531\u57fa\u542b\u91cf\u4e0b\u964d\uff0c\u540c\u65f6\u53ef\u4ee5\u4ece\u8bd5\u9a8c\u7ed3\u679c\u4e2d\u53d1\u73b0\u813e\u810f\u548c\u8840\u6db2\u708e\u75c7\u56e0\u5b50\u53d8\u5316\u57fa\u672c\u8d8b\u52bf\u4e00\u81f4\uff0c\u6297\u708e\u6570\u636e\u4e0e\u813e\u810f\u548c\u8840\u6db2\u6297\u6c27\u5316\u6307\u6807\u5448\u6b63\u76f8\u5173\uff0c\u800c\u4e14\u8fde\u7fd8\u916f\u82f7A \u548cLPS\u7684\u6d3b\u6027\u53d7\u5e94\u7528\u5242\u91cf\u548c\u4f5c\u7528\u65f6\u95f4\u7684\u5f71\u54cd\uff0c\u8fd9\u8868\u660e\u9e21\u4f53\u514d\u75ab\u7cfb\u7edf\u5728\u8fde\u7fd8\u916f\u82f7A\u548cLPS\u4f5c\u7528\u9e21\u65f6\u88ab\u6fc0\u6d3b\uff0c\u8840\u6db2\u548c\u813e\u810f\u5728\u751f\u7269\u5b66\u6548\u5e94\u65b9\u9762\u5bf9\u673a\u4f53\u7684\u514d\u75ab\u673a\u5236\u53d1\u6325\u7740\u91cd\u8981\u7684\u4f5c\u7528\uff0c\u6765\u9002\u5e94\u9e21\u4f53\u514d\u75ab\u8c03\u8282\u673a\u5236\uff0c\u6700\u7ec8\u8d77\u5230\u6297\u6c27\u5316\u548c\u6297\u708e\u529f\u80fd\u3002<\/p>\n

\u674e\u5bd2\u51b0\u7b49\u91c7\u7528\u6e05\u9664\u6709\u673a\u81ea\u7531\u57faDPPH\u6cd5\u8bc4\u4ef7\u8fde\u7fd84 \u79cd\u4e0d\u540c\u89c4\u683c\u5546\u54c1\u7684\u6297\u6c27\u5316\u6027\uff0c\u53d1\u73b0\u9752\u7fd8\u5fc3\u6297\u6c27\u5316\u4f5c\u7528\u6700\u5f3a\uff0c\u4e14\u8fde\u7fd8\u7684\u6297\u6c27\u5316\u6027\u4e0e\u8fde\u7fd8\u916f\u82f7A \u548c\u69f2\u76ae\u7d20\u7684\u542b\u91cf\u6709\u5173\u3002<\/p>\n

Jiao \u7b49\u5bf9\u8fde\u7fd8\u6325\u53d1\u6cb9\u03b2-\u80e1\u841d\u535c\u7d20\/\u4e9a\u6cb9\u9178\u68c0\u6d4b\u7ed3\u679c\u4e3a63\uff0e56%\uff0c\u8bf4\u660e\u5176\u6709\u4e00\u5b9a\u7684\u6297\u6c27\u5316\u6d3b\u6027\u3002<\/p>\n

Gai \u7b49\u5229\u7528DPPH \u548c\u03b2-\u80e1\u841d\u535c\u7d20\/\u4e9a\u6cb9\u9178\u4f53\u7cfb\u6d4b\u8bd5\u8fde\u7fd8\u79cd\u5b50\u6325\u53d1\u6cb9\u6297\u6c27\u5316\u6d3b\u6027\uff0c\u5176IC50<\/sub>\u503c\u5206\u522b\u4e3a22. 35\u300129. 51 mg \/mL\u3002<\/p>\n

\u738b\u91d1\u6885\u7b49\u91c7\u7528SPME \u6280\u672f\u5728\u8fde\u7fd8\u82b1\u4e2d\u68c0\u6d4b\u52302\uff0c6-\u4e8c\u53d4\u4e01\u57fa\u5bf9\u7532\u915a( 1. 21%)\u548c2\uff0c6-\u4e8c-\u53d4\u4e01\u57fa-4-(
\n1-\u6c27\u4ee3\u4e19\u57fa) \u82ef\u915a( 6. 99%)\uff0c2\uff0c6-\u4e8c\u53d4\u4e01\u57fa\u5bf9\u7532\u915a\u662f\u76ee\u524d\u4f7f\u7528\u8303\u56f4\u6700\u5e7f\u3001\u7528\u91cf\u6700\u5927\u7684\u6297\u6c27\u5316\u5242\uff0c\u4fd7\u79f0\u6297\u6c27\u5316\u5242264\u3002<\/p>\n

\u9b4f\u5e0c\u9896\u91c7\u7528DPPH \u548c\u90bb\u82ef\u4e09\u915a\u81ea\u6c27\u5316\u6cd5\u53d1\u73b0\u8fde\u7fd8\u79cd\u5b50\u6325\u53d1\u6cb9\u5bf9\u6709\u673a\u81ea\u7531\u57fa( DPPH) \u548c\u8d85\u6c27\u9634\u79bb\u5b50\u81ea\u7531\u57fa( O2<\/sub>–<\/sup>\u00b7) \u5747\u6709\u663e\u8457\u6e05\u9664\u4f5c\u7528\uff0c\u6e05\u9664\u7387\u5927\u4e8e90%\u3002\u4e0a\u8ff0\u7814\u7a76\u8868\u660e\u8fde\u7fd8\u82b1\u4e0e\u79cd\u5b50\u5747\u5177\u6709\u4f5c\u4e3a\u5929\u7136\u6297\u6c27\u5316\u5242\u7684\u5f00\u53d1\u6f5c\u8d28\u3002<\/p>\n

\u6d82\u79cb\u4e91\u8003\u5bdf\u4e86\u81ea\u5236\u7684\u8fde\u7fd8\u603b\u6728\u8102\u7d20\u63d0\u53d6\u7269\uff08\u6728\u8102\u7d20\u542b\u91cf93.5%\uff09\u5bf9\u8d85\u9634\u79bb\u5b50\u81ea\u7531\u57fa\u3001\u7f9f\u81ea\u7531\u57fa\u3001\u8102\u8fc7\u6c27\u5316\u7269\u81ea\u7531\u57fa\u3001\u8fc7\u6c27\u5316\u6c22\u3001\u4e9a\u785d\u9178\u94a0\u7b49\u5404\u79cd\u81f4\u8870\u8001\u81ea\u7531\u57fa\u7684\u6291\u5236\u6e05\u9664\u4f5c\u7528\u3002\u5b9e\u9a8c\u663e\u793a\uff0c\u8fde\u7fd8\u6728\u8102\u7d20\u63d0\u53d6\u7269\u5bf9\u6e05\u9664\u7f9f\u57fa\u81ea\u7531\u57fa\u6709\u6781\u5f3a\u7684\u4f5c\u7528\uff0c\u5f53\u6d53\u5ea6\u7ea6\u4e3a0.6 mg\/mL \u65f6\uff0c\u5bf9\u7f9f\u57fa\u81ea\u7531\u57fa\u7684\u6e05\u9664\u6548\u679c\u8fbe\u523090% \u4ee5\u4e0a\uff1b\u5bf9\u8d85\u6c27\u9634\u79bb\u5b50\u81ea\u7531\u57fa\u6709\u4e00\u5b9a\u7684\u6e05\u9664\u80fd\u529b\uff0c\u5f53\u6d53\u5ea6\u7ea6\u4e3a2.0 mg\/mL \u65f6\uff0c\u5bf9\u8d85\u6c27\u9634\u79bb\u5b50\u81ea\u7531\u57fa\u7684\u6e05\u9664\u6548\u679c\u8fbe\u523090% \u4ee5\u4e0a\uff0c\u4f46\u6e05\u9664\u7387\u4e0e\u6728\u8102\u7d20\u63d0\u53d6\u7269\u7684\u6d53\u5ea6\u4e0d\u5448\u7ebf\u6027\u5173\u7cfb\uff1b\u603b\u6728\u8102\u7d20\u63d0\u53d6\u7269\u8fd8\u80fd\u591f\u6709\u6548\u6e05\u9664\u8102\u8fc7\u6c27\u5316\u7269\u81ea\u7531\u57fa\uff0c\u6291\u5236\u732a\u6cb9\u9178\u8d25\uff0c\u5728\u9ad8\u6e29\u6761\u4ef6\u4e0b\u653e\u51650.05% \u5c31\u80fd\u5c06\u732a\u6cb9\u4e2d\u8fc7\u6c27\u5316\u7269\u7684\u542b\u91cf\u63a7\u5236\u5728\u5f88\u4f4e\u7684\u7a0b\u5ea6\uff0c\u800c\u4e14\u6301\u7eed\u6291\u5236\u65f6\u95f4\u5f88\u957f\uff1b\u5f53\u603b\u6728\u8102\u7d20\u63d0\u53d6\u7269\u6d53\u5ea6\u8fbe\u5230150 \u03bcg\/ml\uff0c\u5bf9\u8fc7\u6c27\u5316\u6c22\u7684\u6e05\u9664\u7387\u8fbe87.3%\uff1b\u5bf9\u4e9a\u785d\u9178\u94a0\u7684\u6e05\u9664\u6548\u679c\u4e0d\u4f73\u3002<\/p>\n

\u5b8b\u851a\u5b9e\u9a8c\u53d1\u73b0\uff0c\u8fde\u7fd8\u8102\u7d20\u53ef\u4ee5\u8fc5\u901f\u6e05\u9664DPPH \u548cABTS+\u81ea\u7531\u57fa\uff0c\u5176ABTS+ \u81ea\u7531\u57fa\u6e05\u9664\u6d3b\u6027\u9ad8\u4e8e\u6297\u574f\u8840\u9178\u3002<\/p>\n

\u6734\u9999\u5170\u7b49\u5bf9\u4ece\u8fde\u7fd8\u679c\u5b9e\u4e2d\u5206\u79bb\u5230\u7684\u4e24\u4e2a\u65b0\u7684\u6728\u8102\u7d20\u7c7b\u5355\u4f53forsythialanA \u548c forsythialanB \u8fdb\u884c\u4e86\u4f53\u5916\u6297\u6c27\u5316\u7814\u7a76\uff0c\u7ed3\u679c\u53d1\u73b0\u4e24\u8005\u5747\u80fd\u660e\u663e\u4fdd\u62a4 SIN-1 \u8bf1\u5bfc\u7684LLC-PK1 \u6c27\u5316\u635f\u4f24\uff08P<0.01\uff09\u3002<\/p>\n

\u90b1\u667a\u519b\u7b49\u5236\u5907\u5f97\u5230\u7eaf\u5ea6\u4e3a93% \u8fde\u7fd8\u82f7\u548c92%\u8fde\u7fd8\u916f\u82f7A\uff0c\u8fde\u7fd8\u82f7\u548c\u8fde\u7fd8\u916f\u82f7A \u5bf9\u732a\u6cb9\u6c27\u5316\u4f5c\u7528\u5747\u6709\u4e00\u5b9a\u7684\u6291\u5236\u4f5c\u7528\uff0c\u4e14\u968f\u7740\u5242\u91cf\u7684\u589e\u52a0\uff0c\u6291\u5236\u6548\u679c\u8d8a\u660e\u663e\u3002\u5176\u4e2d\uff0c\u8fde\u7fd8\u916f\u82f7A \u5bf9\u6cb9\u8102\u6c27\u5316\u7684\u6291\u5236\u4f5c\u7528\u8fdc\u4f18\u4e8e\u8fde\u7fd8\u82f7\uff0c\u6548\u679c\u4e0e\u9633\u6027\u5bf9\u71672\uff0c6- \u4e8c\u53d4\u4e01\u57fa-4- \u7532\u57fa\u82ef\u915a\u63a5\u8fd1\uff0c\u6b64\u5916\uff0cVC \u548c\u67e0\u6aac\u9178\u5bf9\u8fde\u7fd8\u82f7\u548c\u8fde\u7fd8\u916f\u82f7A \u7684\u6297\u6c27\u5316\u4f5c\u7528\u5747\u8868\u73b0\u51fa\u534f\u8c03\u589e\u6548\u4f5c\u7528\u3002<\/p>\n

\u9f50\u5efa\u7ea2\u5b9e\u9a8c\u53d1\u73b0\u8fde\u7fd8\u82f7\u5bf94 \u79cd\u690d\u7269\u6cb9\u8102\u5747\u6709\u8f83\u5f3a\u7684\u6297\u6c27\u5316\u6d3b\u6027\uff0c \u5176\u4e2d\u5bf9\u82b1\u751f\u6cb9\u5177\u6709\u8f83\u5f3a\u7684\u6297\u6c27\u5316\u6d3b\u6027\uff0c \u5176\u6b21\u4e3a\u8475\u82b1\u7c7d\u6cb9\u3001\u5927\u8c46\u6cb9\u548c\u83dc\u7c7d\u6cb9\uff0c\u800c\u4e14\uff0c\u6dfb\u52a0\u8fde\u7fd8\u82f7\u6297\u6c27\u5316\u6548\u679c\u660e\u663e\u8981\u4f18\u4e8eBHT\u3002<\/p>\n

\u66f2\u6b22\u6b22\u7b49\u548c\u5f20\u9896\u5a1f\u7b49\u5b9e\u9a8c\u53d1\u73b0\u8fde\u7fd8\u82f7\u6e05\u9664DPPH \u81ea\u7531\u57fa\u7684\u80fd\u529b\u8f83\u5f31\u3002<\/p>\n

2.2 \u6297\u83cc\u6d3b\u6027<\/p>\n

\u8fde\u7fd8\u5bf9\u5927\u80a0\u6746\u83cc\u3001\u5927\u80a0\u57c3\u5e0c\u83cc\u3001\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u7b49\u591a\u79cd\u9769\u5170\u9633\u6027\u83cc\u3001\u9769\u5170\u9634\u6027\u83cc\u548c\u7ed3\u6838\u6746\u83cc\u90fd\u6709\u6291\u5236\u4f5c\u7528\u3002<\/p>\n

\u59dc\u9192\u7b49\u4ece\u8fde\u7fd8\u679c\u5b9e\u4e2d\u5206\u79bb\u5f97\u52309\u79cd\u5316\u5408\u7269\uff0c\u91c7\u7528\u4e8c\u500d\u7a00\u91ca\u6cd5\u6d4b \u5b9a\u5206\u79bb\u51fa\u7684\u5316\u5408\u7269\u7684\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u3001\u5927\u80a0\u57c3\u5e0c\u83cc\u3001\u7caa\u80a0\u7403\u83cc \u7684\u6297\u83cc\u6d3b\u6027\u3002\u5b9e\u9a8c\u7ed3\u679c\u8868\u660e\uff0c\u5206\u79bb\u51fa\u7684\u90e8\u5206\u5316\u5408\u7269\u5bf9\u91d1\u9ec4\u8272\u8461 \u8404\u7403\u83cc\u751f\u957f\u5177\u6709\u4e00\u5b9a\u7684\u6291\u5236\u4f5c\u7528\u3002<\/p>\n

QU H\u7b49\u91c7\u7528\u5fae\u91cf\u5e73\u677f\u6cd5\u6d4b\u5b9a\u4e86\u8fde\u7fd8\u916f\u82f7\u548c\u8fde\u7fd8\u82f7\u7684\u4f53\u5916\u6297\u83cc\u6d3b\u6027\uff0c\u7ed3\u679c\u8868\u660e\uff0c\u4e0d\u540c\u6d53\u5ea6\u7684\u8fde\u7fd8\u916f\u82f7\u548c\u8fde\u7fd8\u82f7\u5bf9\u5927\u80a0\u6746\u83cc\u3001\u94dc\u7eff\u5047\u5355\u80de\u83cc\u548c\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u5747\u6709\u8f83\u5f3a\u7684\u6297\u83cc\u6d3b\u6027\u3002<\/p>\n

\u9648\u747e\u7b49\u91c7\u7528K\uff0dB\u7eb8\u7247\u6269\u6563\u6cd5\u8003\u5bdf\u548c\u6bd4\u8f83\u8fde\u7fd8\u6c34\u714e\u6db2\u7684\u6297\u83cc\u6d3b\u6027\uff0c\u5b9e\u9a8c\u83cc\u6709\u5927\u80a0\u6746\u83cc\u3001\u67af\u8349\u6746\u83cc\u3001\u80ba\u708e\u53cc\u7403\u83cc\u3001\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u3001\u767d\u8272\u5ff5\u73e0\u83cc\uff0c\u5b9e\u9a8c\u7ed3\u679c\u8868\u660e\u8fde\u7fd8\u6c34\u714e\u6db2\u5bf9\u4e0a\u8ff05\u79cd\u8bd5\u9a8c\u83cc\u90fd\u8868\u73b0\u51fa\u4e0d\u540c\u7a0b\u5ea6\u7684\u6291\u5236\u4f5c\u7528\u3002<\/p>\n

\u5f02\u8fde\u7fd8\u916f\u82f7\u548c\u8fde\u7fd8\u916f\u82f7\u5747\u6709\u8f83\u5f3a\u7684\u6291\u83cc\u80fd\u529b\uff0c\u5c24\u5176\u5bf9\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\uff08SA\uff09\u7684\u6291\u5236\u4f5c\u7528\u6bd4\u56db\u73af\u7d20\u8fd8\u5f3a\uff0c\u662f\u8fde\u7fd8\u4e2d\u6781\u6709\u524d\u666f\u7684\u690d\u7269\u6297\u83cc\u6d3b\u6027\u6210\u5206\u3002\u836f\u7406\u5b9e\u9a8c\u8868\u660e\uff0c\u8fde\u7fd8\u6c34\u714e\u6db2\u6709\u5e7f\u8c31\u7684\u6291\u83cc\u4f5c\u7528\uff0c\u5bf9\u591a\u79cd\u9769\u5170\u9633\u6027\u53ca\u9634\u6027\u7ec6\u83cc\u5747\u6709\u660e\u663e\u7684\u6291\u5236\u4f5c\u7528\u30022009 \u5e74\u76db\u5c0a\u6765\u7814\u7a76\u53d1\u73b0\uff0c\u5176\u6291\u83cc\u6d3b\u6027\u90e8\u4f4d\u4e3a30\uff05\u4e59\u9187\u300150\uff05\u4e59\u9187\u90e8\u4f4d\u3002\u53e6\u7814\u7a76\u8868\u660e\uff0c\u5176\u6291\u83cc\u6d3b\u6027\u6210\u5206\u4e3b\u8981\u4e3a\u80de\u5185\u4ee3\u8c22\u4ea7\u7269\uff08\u5b58\u5728\u4e8e\u83cc\u4e1d\u4f53\u7c97\u63d0\u7269\u4e2d\uff09\u3002<\/p>\n

\u8fde\u7fd8\u5c5e\u690d\u7269\u4e2d\u7684\u5496\u5561\u9170\u7cd6\u82f7\u7c7b\u6210\u5206\u8fde\u7fd8\u916f\u82f7\u3001\u8fde\u7fd8\u79cd\u82f7\u7b49\u5747\u6709\u5f88\u5f3a\u7684\u6297\u83cc\u6d3b\u6027\uff0c\u5176\u4e59\u9187\u63d0\u53d6\u7269\u6297\u83cc\u8c31\u5e7f\uff0c\u5bf9\u591a\u79cd\u9769\u5170\u9633\u6027\u83cc\u3001\u9634\u6027\u83cc\u5747\u6709\u6291\u5236\u4f5c\u7528\u3002\u5176\u714e\u5242\u7684\u6700\u4f4e\u6291\u83cc\u6d53\u5ea6(MIC)\u5206\u522b\u4e3a\uff1a\u5fd7\u8d3a\u6c0f\u75e2\u75be\u6746\u83cc\u3001\u53f2\u6c0f\u75e2\u75be\u6746\u83cc\u3001\u9f20\u75ab\u6746\u83cc\u3001\u4eba\u578b\u7ed3\u6838\u6746\u83cc1\uff1a640\uff1b\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u3001\u4f24\u5bd2\u6746\u83cc\u3001\u970d\u4e71\u5f27\u83cc1\uff1a320\uff1b\u80ba\u708e\u53cc\u7403\u83cc\u3001\u526f \u5bd2\u6746\u83cc\u30011\uff1a160\uff1b\u6eb6\u8840\u6027\u94fe\u7403\u83cc\u3001\u798f\u6c0f\u75e2\u75be\u6746\u83cc\u3001\u5927\u80a0\u57c3\u5e0c\u6c0f\u83cc\u3001\u53d8\u5f62\u6746\u83cc\u3001\u767d\u5589\u6746\u83cc1\uff1a80\u3002\u8fde\u7fd8\u915a\u4e3a\u5176\u6297\u83cc\u7684\u4e3b\u8981\u6210\u5206\uff0c\u5bf9\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u53ca\u5fd7\u8d3a\u6c0f\u75e2\u75be\u6746\u83cc\u7684MIC\u5206\u522b\u4e3a1\uff1a5120\u53ca1\uff1a1280\u3002\u8fde\u7fd8\u5b50\u6325\u53d1\u6cb9\u5728\u4f53\u5916\u5bf9\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u4e5f\u6709\u660e\u663e\u7684\u6297\u83cc\u4f5c\u7528MIC\u4e3a1\uff1a1024\uff0c\u5176\u6297\u83cc\u4f5c\u7528\u7a33\u5b9a\u800c\u5f7b\u5e95\u3002<\/p>\n

\u5218\u7389\u5a55\u7b49\u7814\u7a76\u8868\u660e\uff0c\u8fde\u7fd8\u548c\u91d1\u94f6\u82b1\u914d\u4f0d\u4f7f\u7528\u8010\u7532\u6c27\u897f\u6797\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\uff08MDRMRSA\uff09\u7684\u6d3b\u6027\u6700\u9ad8\uff0c\u8fde\u7fd8\u6b21\u4e4b\uff0c\u91d1\u94f6\u82b1\u6700\u4f4e\u3002\u8fde\u7fd8\u63d0\u53d6\u7269\u4e2d\u8fde\u7fc5\u82f7\u53ef\u4ee5\u6291\u5236\u7532\u578b\u6d41\u611f\u75c5\u6bd2\u6838\u86cb\u767d\uff08NP\uff09\u57fa\u56e0\u7684\u8f6c\u67d3\u540e\u8868\u8fbe\uff0c\u4ece\u800c\u8fbe\u5230\u6297\u201c\u7532\u6d41\u201d\u7684\u4f5c\u7528\uff0c\u51763 \u79cd\u4e0d\u540c\u5242\u578b\u4e2d\uff0c\u4ee5\u8fde\u7fd8\u82f7\u6291\u5236NP \u57fa\u56e0\u8868\u8fbe\u91cf\u6700\u5f3a\uff0c\u8fde\u7fd8\u9187\u63d0\u6db2\u6b21\u4e4b\uff0c\u8fde\u7fd8\u6c34\u63d0\u6db2\u6700\u5f31\u3002<\/p>\n

\u59dc\u6d9b\u7b49\u901a\u8fc7\u5efa\u7acb\u8fde\u7fd8\u6307\u7eb9\u56fe\u8c31\u4e0e\u6297\u83cc\u6d3b\u6027\u4e4b\u95f4\u7684\u8c31\u6548\u5173\u7cfb\uff0c\u53d1\u73b0\u5728\u8fde\u7fd8\u6307\u7eb9\u56fe\u8c31\u4e2d\uff0c\u8fde\u7fd8\u916f\u82f7A\u3001\u5496\u5561\u9178\u3001\u8fde\u7fd8\u82f7\u548c\u8fde\u7fd8\u8102\u7d20\u5f71\u54cd\u8fde\u7fd8\u5bf9\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u6d3b\u6027\u7684\u6291\u5236\u4f5c\u7528\u3002\u8fde\u7fd8\u6291\u83cc\u7684\u6d3b\u6027\u6210\u5206\u53ef\u80fd\u4e3a\u8fde\u7fd8\u916f\u82f7\u3001\u5496\u5561\u9178\u3001\u8fde\u7fd8\u82f7\u548c\u8fde\u7fd8\u8102\u7d20\u3002<\/p>\n

\u8fde\u7fd8\u6325\u53d1\u6cb9\u5bf9\u8461\u8404\u7403\u83cc\u3001\u82bd\u5b62\u6746\u83cc\u3001\u5927\u80a0\u6746\u83cc\u3001\u7eff\u8113\u6746\u83cc\u3001\u767d\u8272\u5ff5\u73e0\u83cc\u3001\u9ed1\u66f2\u9709\u83cc\u7684MIC \u503c\u4e3a0\uff0e78\uff5e 6\uff0e25 mg \/mL\uff0c\u663e\u793a\u8fde\u7fd8\u6325\u53d1\u6cb9\u5177\u6709\u5e7f\u8c31\u6297\u83cc\u4f5c\u7528\u3002<\/p>\n

\u9b4f\u5e0c\u9896\u7684\u7814\u7a76\u8868\u660e\uff0c\u8fde\u7fd8\u79cd\u5b50\u6325\u53d1\u6cb9\u5bf9\u917f\u9152\u9175\u6bcd\u7b493 \u79cd\u5e38\u89c1\u771f\u83cc\u548c\u8461\u8404\u9752\u9709\u7b493 \u79cd\u8461\u8404\u81f4\u75c5\u771f\u83cc\u540c\u6837\u6709\u6291\u5236\u4f5c\u7528\uff0c\u5176\u6291\u83cc\u673a\u7406\u53ef\u80fd\u662f\u901a\u8fc7\u7834\u574f\u5927\u80a0\u6746\u83cc\u3001\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u548c\u917f\u9152\u9175\u6bcd3 \u79cd\u6307\u793a\u83cc\u7684\u7ec6\u80de\u58c1\u548c\u7ec6\u80de\u819c\u7ed3\u6784\uff0c\u5bfc\u81f4\u7ec6\u80de\u5185\u5bb9\u7269\u7684\u5916\u6f0f\uff0c\u4ece\u800c\u5f15\u8d77\u7ec6\u80de\u7684\u51cb\u4ea1; \u7834\u574f\u7ec6\u83cc\u751f\u957f\u8fc7\u7a0b\u4e2d\u67d0\u4e9b\u86cb\u767d\u8d28\u6216\u86cb\u767d\u8d28\u5408\u6210\u9176\uff0c\u5bfc\u81f4\u7ec6\u80de\u751f\u957f\u5468\u671f\u7684\u7ec8\u65ad\u800c\u6b7b\u4ea1\u3002<\/p>\n

Guo \u7b49\u7814\u7a76\u8fdb\u4e00\u6b65\u8868\u660e\u8fde\u7fd8\u6325\u53d1\u6cb9\u5bf9\u5927\u80a0\u6746\u83cc\u548c\u8461\u8404\u7403\u83cc\u5747\u5177\u6709\u8f83\u5f3a\u7684\u6291\u5236\u4f5c\u7528\uff0c\u4f5c\u7528\u5f3a\u5f31\u4e0e\u6d53\u5ea6\u3001\u4f5c\u7528\u65f6\u95f4\u5448\u6b63\u76f8\u5173\uff0c\u6291\u5236\u4f5c\u7528\u662f\u6539\u53d8\u7ec6\u83cc\u7684\u819c\u901a\u900f\u6027\uff0c\u5176\u673a\u5236\u53ef\u80fd\u662f\u901a\u8fc7\u7834\u574f\u5927\u80a0\u6746\u83cc28KDa \u86cb\u767d\u8d28\u3001\u8461\u8404\u7403\u83cc52 KDa \u86cb\u767d\u8d28\u7b49\u7ec6\u80de\u58c1\u5173\u952e\u529f\u80fd\u86cb\u767d\u8d28\uff0c\u4ece\u800c\u5bfc\u81f4\u7ec6\u80de\u5185\u6210\u5206\u6e17\u6f0f\uff0c\u7279\u522b\u662fK +<\/sup>\u3001Ca2 +<\/sup>\u3001Na +<\/sup> \u7b49\u7535\u89e3\u8d28\u5927\u91cf\u6d41\u5931\uff0c\u4f7f\u7ec6\u80de\u6b7b\u4ea1\uff0c\u5fae\u751f\u7269\u5931\u6d3b\u3002<\/p>\n

\u9b4f\u73ca\u7b49\u7814\u7a76\u8868\u660e\u4e0d\u540c\u4ea7\u5730\u8fde\u7fd8\u6325\u53d1\u6cb9\u5bf9\u591a\u79cd\u7ec6\u83cc\u5747\u6709\u6291\u5236\u4f5c\u7528\uff0c\u6291\u83cc\u6d3b\u6027\u4e0e\u836f\u6750\u7684\u4ea7\u5730\u3001\u6279\u6b21\u3001\u8d2e\u5b58\u65f6\u95f4\u6709\u5173\u3002<\/p>\n

Jiao \u7b49\u91c7\u7528\u6c34\u84b8\u6c14\u84b8\u998f\u6cd5( HD) \u3001\u5fae\u535a\u6c34\u84b8\u6c14\u84b8\u998f\u6cd5( MHD) \u53ca\u9176\u8f85\u52a9\u5fae\u6ce2\u6c34\u84b8\u6c14\u6cd5( EAMHD) \u63d0\u53d6\u8fde\u7fd8\u679c\u5b9e\u7684\u6325\u53d1\u6cb9\uff0c\u53d1\u73b0EAMHD \u83b7\u5f97\u7684\u6325\u53d1\u6cb9\u6d3b\u6027\u6700\u5f3a\uff0c\u53ef\u80fd\u7531\u4e8e\u84b8\u998f\u65f6\u95f4\u8f83\u77ed\uff0c\u6c34\u89e3\u4f5c\u7528\u51cf\u5c11\uff0c\u542b\u6c27\u5355\u841c\u7c7b\u5316\u5408\u7269\u4e0e\u5fae\u6ce2\u76f8\u4e92\u4f5c\u7528\u66f4\u6613\u63d0\u53d6\u3002<\/p>\n

\u6731\u6885\u82b3\u7b49\u6291\u83cc\u5b9e\u9a8c\u8868\u660e\u5355\u5473\u6325\u53d1\u6cb9\u53ca\u5355\u63d0\u6df7\u5408\u6325\u53d1\u6cb9\u5bf9\u591a\u79cd\u7ec6\u83cc\u7684\u6291\u83cc\u6548\u679c\u5747\u4f18\u4e8e\u914d\u4f0d\u63d0\u53d6\u6325\u53d1\u6cb9\u7ec4\u3002\u4e0a\u8ff0\u7814\u7a76\u8868\u660e\uff0c\u8fde\u7fd8\u6325\u53d1\u6cb9\u6291\u83cc\u6d3b\u6027\u5f3a\u5f31\u53d7\u836f\u6750\u4ea7\u5730\u3001\u8d2e\u5b58\u3001\u63d0\u53d6\u65b9\u6cd5\u53ca\u914d\u4f0d\u7b49\u60c5\u51b5\u5f71\u54cd\u3002<\/p>\n

\u674e\u5fb7\u6210\u7b49\u7528\u8fde\u7fd8\u7684\u63d0\u53d6\u6db2\uff0c\u91c7\u7528\u7eb8\u7247\u6cd5\uff0c\u4ee5\u6291\u83cc\u5708\u5927\u5c0f\u4e3a\u53c2\u8003\u6307\u6807\uff0c\u89c2\u5bdf\u6291\u83cc\u6548\u679c\u3002\u7814\u7a76\u7ed3\u679c\u663e\u793a\u6c34\u63d0\u6db2\uff0c30\uff05 \u300150\uff05\u300170\uff05 \u300195\uff05\u4e59\u9187\u63d0\u53d6\u6db2\u7684\u6291\u83cc\u5708\u5206\u522b\u4e3a9\uff0e5\uff0c10\uff0e0\uff0c12\uff0e2\uff0c9\uff0e3\uff0c10\uff0e1 mm\u3002<\/p>\n

\u9a6c\u96ea\u6885\u7b49\u7528\u4e0d\u540c\u6781\u6027\u7684\u6eb6\u5242\u63d0\u53d6\u8fde\u7fd8\uff0c\u7528K-B\u7eb8\u7247\u6269\u6563\u6cd5\u5bf9\u63d0\u53d6\u7269\u7684\u6291\u83cc\u6d3b\u6027\u8fdb\u884c\u4e86\u521d\u6b65\u7814\u7a76\uff0c\u7814\u7a76\u7ed3\u679c\u8868\u660e\uff0c\u8fde\u7fd8\u7684\u4e59\u9178\u4e59\u916f\u63d0\u53d6\u6db2\u5177\u6709\u6291\u5236\u5927\u80a0\u6746\u83cc\u7684\u4f5c\u7528\u3002<\/p>\n

\u8679\u97ec\u7b49\u7528\u8fde\u7fd8\u63d0\u53d6\u6db2\uff0c\u91c7\u7528\u7eb8\u7247\u6cd5\uff0c\u4ee5\u6291\u83cc\u5708\u5927\u5c0f\u4e3a\u53c2\u8003\u6307\u6807\uff0c\u7814\u7a76\u7ed3\u679c\u663e\u793a\u6c34\u63d0\u6db2\uff0c30\uff05 \u300150\uff05 \u300170\uff05 \u300195\uff05 \u4e59\u9187\u63d0\u53d6\u6db2\u5bf9\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u7684\u6291\u83cc\u5708\u5206\u522b\u4e3a15\uff0c16\uff0e33\uff0c17\uff0e0\uff0c17\uff0e31\uff0c19\uff0e07mm\u3002<\/p>\n

\u5f90\u5764\u91c7\u7528\u6ee4\u7eb8\u7247\u6269\u6563\u6cd5\u6d4b\u5b9a\u6291\u83cc\u6548\u679c\uff0c\u7ed3\u679c\u8868\u660e\u8fde\u7fd8\u6325\u53d1\u6cb9\u5bf9\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u7684\u6291\u83cc\u5708\u5927\u4e8e10mm\uff0c\u5177\u6709\u4e2d\u3001\u5f3a\u5ea6\u6297\u83cc\u6d3b\u6027\u3002\u7a0b\u6653\u8389\u7b49 \u7528\u8fde\u7fd8\u4e59\u9187\u63d0\u53d6\u7269\u8179\u8154\u6ce8\u5c04\u611f\u67d3\u9f20\u4f24\u5bd2\u7684\u5c0f\u9f20\uff0c\u89c2\u5bdf\u5c0f\u9f20\u7684\u751f\u5b58\u65f6\u95f4\uff0c\u813e\u810f\u7684\u91cd\u91cf\u6307\u6570\uff0c\u810f\u5668\u83cc\u843d\u8ba1\u6570\uff0c\u68c0\u6d4b\u8840\u6e05\u4e2d\u6297\u4f53IgG\u6c34\u5e73\uff0c\u7528ELISA\u6cd5\u6d4b\u5b9a\u5c0f\u9f20\u813e\u810f\u91ca\u653e\u7684\u7ec6\u80de\u56e0\u5b50\uff0c\u7ed3\u679c\u663e\u793a\u7ecf\u8fde\u7fd8\u4e59\u9187\u63d0\u53d6\u7269\u6cbb\u7597\u7684\u5c0f\u9f20\uff0c\u4e0e\u5bf9\u7167\u7ec4\u76f8\u6bd4\uff0c\u751f\u5b58\u65f6\u95f4\u5747\u5ef6\u957f\uff0c\u813e\u810f\u91cd\u91cf\u6307\u6570\u5747\u964d\u4f4e\uff0c\u813e\u810f\u83cc\u843d\u8ba1\u6570\u5747\u964d\u4f4e\uff0c\u8840\u6e05\u4e2d\u6297\u4f53IgG\u6c34\u5e73\u5747\u5347\u9ad8\uff0c\u813e\u810f\u7ec6\u80de\u5206\u6ccc\u7ec6\u80de\u56e0\u5b50IFN-\u03b3\u6c34\u5e73\u5747\u4e0a\u5347\u3002\u8bf4\u660e\u8fde\u7fd8\u4e59\u9187\u63d0\u53d6\u7269\u80fd\u591f\u901a\u8fc7\u589e\u5f3a\u6297\u4f53\u7684\u4ea7\u751f\uff0c\u8c03\u8282\u7ec6\u80de\u56e0\u5b50\u7684\u91ca\u653e\uff0c\u589e\u5f3a\u5bbf\u4e3b\u7684\u4f53\u6db2\u514d\u75ab\u548c\u7ec6\u80de\u514d\u75ab\u529f\u80fd\uff0c\u4fdd\u62a4\u5bbf\u4e3b\u62b5\u6297\u80de\u5185\u83cc\u7684\u611f\u67d3\u3002<\/p>\n

\u4efb\u73b2\u73b2\u7b49\u7528\u8fde\u7fd8\u5e72\u7c89\u7684\u53cc\u84b8\u6c34\u6eb6\u6db2\u4f5c\u7528\u4e8e\u591a\u91cd\u8010\u836f\u6027\u5927\u80a0\u57c3\u5e0c\u83cc\uff0c\u901a\u8fc7\u5bf9\u8fde\u7fd8\u4f5c\u7528\u540e\u7684\u591a\u91cd\u8010\u836f\u6027\u5927\u80a0\u57c3\u5e0c\u83ccAcrA\u57fa\u56e0\u4ea7\u7269\u7684\u5e8f\u5217\u5206\u6790\uff0c\u53ef\u4ee5\u770b\u51fa\u591a\u91cd\u8010\u836f\u6027\u5927\u80a0\u57c3\u5e0c\u83ccAcrA\u57fa\u56e0\u53d1\u751f\u4e8624\u4e2a\u78b1\u57fa\u7a81\u53d8\uff0c\u8bf4\u660e\u8fde\u7fd8\u80fd\u591f\u6539\u53d8\u5927\u80a0\u57c3\u5e0c\u83ccAcrA\u57fa\u56e0\u7684\u5916\u6392\u7cfb\u7edf\u7ed3\u6784\uff0c\u4f7f\u5927\u80a0\u57c3\u5e0c\u83cc\u5bf9\u73af\u5883\u7684\u9002\u5e94\u80fd\u529b\u53ca\u6297\u83cc\u836f\u8010\u836f\u6c34\u5e73\u4e0b\u964d\uff0c\u4ece\u800c\u8fbe\u5230\u6291\u5236\u5927\u80a0\u57c3\u5e0c\u83cc\u7684\u4f5c\u7528\u3002<\/p>\n

\u9ec4\u745e\u7389\u7b49\u7528\u5bf9\u73af\u4e19\u6c99\u661f\u5916\u6392\u8868\u578b\u548c\u5916\u6392\u6cf5\u7f16\u7801\u57fa\u56e0\u5747\u4e3a\u9633\u6027\u7684\u591a\u91cd\u8010\u836f\u6027\u9c8d\u66fc\u4e0d\u52a8\u6746\u83cc\u4f5c\u4e3a\u5b9e\u9a8c\u83cc\u682a\uff0c\u5e94\u7528\u743c\u8102\u5bf9\u500d\u7a00\u91ca\u6cd5\u68c0\u6d4b\u8fde\u7fd8\u6c34\u714e\u5242\u4f5c\u7528\u524d\u540e\u73af\u4e19\u6c99\u661f\u5bf9\u7ec6\u83cc\u7684\u6700\u5c0f\u6291\u83cc\u6d53\u5ea6(MIC)\uff0c\u9009\u53d6\u4f5c\u7528\u524d\u540eMIC\u53d8\u5316\u8f83\u5927\u7684\u4ee3\u8868\u83cc\u682a\u8fdb\u884c\u57fa\u56e0\u6d4b\u5e8f\uff0c\u5e76\u5c06\u4f5c\u7528\u524d\u540e\u7684\u6d4b\u5e8f\u7ed3\u679c\u8fdb\u884c\u6bd4\u5bf9\u5206\u6790\u3002\u7ed3\u679c\u663e\u793a\u8fde\u7fd8\u6c34\u714e\u5242\u53ef\u964d\u4f4e\u73af\u4e19\u6c99\u661f\u5bf9\u591a\u91cd\u8010\u836f\u6027\u9c8d\u66fc\u4e0d\u52a8\u6746\u83cc\u7684MIC\uff1b\u6d4b\u5e8f\u5206\u6790\u8868\u660e\uff0c\u8fde\u7fd8\u4f5c\u7528\u540e\u9c8d\u66fc\u4e0d\u52a8\u6746\u83ccadeB\u57fa\u56e0\u5e8f\u5217\u5171\u670912\u4e2a\u78b1\u57fa\u7a81\u53d8\uff1b\u7a81\u53d8\u53d1\u751f\u6c28\u57fa\u9178\u66ff\u6362\u7684\u6709E140V\u3001K145I\u3001A147V\u3001K150N\u3001A151V\u3001K153I\u3002\u8bf4\u660e\u8fde\u7fd8\u6c34\u714e\u5242\u53ef\u5f15\u8d77\u9c8d\u66fc\u4e0d\u52a8\u6746\u83cc\u7684\u4e3b\u52a8\u5916\u6392\u6cf5\u7f16\u7801\u57fa\u56e0ade\u2014ABC\u5e8f\u5217\u53d1\u751f\u7a81\u53d8\uff0c\u6291\u5236\u9c8d\u66fc\u4e0d\u52a8\u6746\u83cc\u7684\u4e3b\u52a8\u5916\u6392\u6cf5\uff0c\u4ece\u800c\u8fbe\u5230\u6291\u5236\u591a\u91cd\u8010\u836f\u6027\u9c8d\u66fc\u4e0d\u52a8\u6746\u83cc\u7684\u4f5c\u7528\u3002<\/p>\n

\u674e\u4ef2\u5174\u7b49\u91c7\u7528\u8fde\u7fd8\u63d0\u53d6\u6db2\u5bf9140\u682a\u8868\u76ae\u8461\u8404\u7403\u83cc\u8fdb\u884c\u4f53\u5916\u6297\u83cc\u6d3b\u6027\u7684\u6d4b\u5b9a\uff0c\u7ed3\u679c\u663e\u793a\u8fde\u7fd8\u5bf9\u8868\u76ae\u8461\u8404\u7403\u83cc\u80fd\u6291\u523650\uff05 \u83cc\u682a\u7684\u6700\u4f4e\u7ec6\u83cc\u6d53\u5ea6(MIC50<\/sub>)\u4e3a0\uff0e122 g\u00b7L-1<\/sup>\uff0c\u6291\u523690\uff05\u83cc\u682a\u7684\u6700\u4f4e\u6291\u83cc\u6d53\u5ea6(MIC50<\/sub>)\u4e3a0\uff0e244 g\u00b7L-1<\/sup>\u3002\u8bf4\u660e\u8fde\u7fd8\u5bf9\u8868\u76ae\u8461\u8404\u7403\u83cc\u5177\u6709\u8f83\u597d\u7684\u4f53\u5916\u6291\u83cc\u6548\u679c\u3002\u5b98\u598d\u7b49\u91c7\u7528\u4f53\u5916\u6784\u5efa\u8868\u76ae\u8461\u8404\u7403\u83cc\u751f\u7269\u819c\u3001XTT\u51cf\u4f4e\u6cd5\u8bc4\u4ef7\u8fde\u7fd8\u82f7\u5bf9\u8868\u76ae\u8461\u8404\u7403\u83cc\u751f\u7269\u819c\u5185\u7ec6\u83cc\u4ee3\u8c22\u7684\u5f71\u54cd\uff0c\u663e\u5fae\u955c\u4e0b\u89c2\u5bdf\u7528\u836f\u540e\u8868\u76ae\u8461\u8404\u7403\u83cc\u751f\u7269\u819c\u5f62\u6001\u548c\u7ed3\u6784\u6539\u53d8\u3002\u7ed3\u679c\u663e\u793a\u8fde\u7fd8\u82f7\u5bf9\u8868\u76ae\u8461\u8404\u7403\u83cc\u751f\u7269\u819c\u83cc\u7684SMIC50<\/sub>\u4e3a31\uff0e25 g\u00b7mL-1<\/sup>\uff0c\u5728\u663e\u5fae\u955c\u4e0b\u89c2\u5bdf\uff0c\u8fde\u7fd8\u82f7\u4f7f\u90e8\u5206\u8868\u76ae\u8461\u8404\u7403\u83cc\u88ab\u819c\u7684\u5f62\u6001\u53d1\u751f\u6539\u53d8\u3002\u8bf4\u660e\u8fde\u7fd8\u82f7\u5bf9\u751f\u7269\u819c\u83cc\u7684\u4ee3\u8c22\u548c\u751f\u7269\u819c\u5f62\u6001\u5747\u6709\u663e\u8457\u5f71\u54cd\u3002<\/p>\n

\u5218\u7389\u5a55\u7b49\u5728\u786e\u5b9a\u91d1\u94f6\u82b1\u3001\u8fde\u7fd8\u53ca\u5176\u914d\u4f0d\u540e\u6297\u591a\u836f\u8010\u836f\u8010\u7532\u6c27\u897f\u6797\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc(MD\uff32\uff0dM\uff32SA) \u7684\u57fa\u7840\u4e0a\uff0c\u63a2\u8ba8\u91d1\u94f6\u82b1\u3001\u8fde\u7fd8\u53ca\u4e8c\u8005\u914d\u4f0d\u540e\u5bf9\u4e34\u5e8a\u5e38\u89c1\u768411\u79cd\u81f4\u75c5\u83cc\u7684\u4f5c\u7528\u3002\u65b9\u6cd5\uff1a\u5c06\u91d1\u94f6\u82b1\u3001\u8fde\u7fd8\u53ca\u5176\u914d\u4f0d\u540e\u6297\u591a\u836f\u8010\u836f\u8010\u7532\u6c27\u897f\u6797\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc(MD\uff32\uff0dM\uff32SA)\u7684\u6700\u4f18\u6d3b\u6027\u7ec4\u5206\uff0c\u5206\u522b\u91c7\u7528\u5fae\u91cf\u7a00\u91ca\u6cd5\u8fdb\u884c\u4f53\u5916\u6297\u83cc\u5b9e\u9a8c\u3002\u7ed3\u679c\uff1a\u91d1\u94f6\u82b1\u3001\u8fde\u7fd8\u914d\u4f0d\u4f7f\u7528\u540e\u5bf911\u79cd\u83cc\u5747\u5177\u6709\u6291\u83cc\u548c\u6740\u83cc\u4f5c\u7528\uff0c\u5176\u4f5c\u7528\u4f18\u4e8e\u5355\u5473\u836f\u548c\u53cc\u9ec4\u8fde\u7c89\u9488\u5242\u3002\u7ed3\u8bba\uff1a\u91d1\u94f6\u82b1\u3001\u8fde\u7fd8\u914d\u4f0d\u4e0d\u4ec5\u5bf9MD\uff32\uff0dM\uff32SA\u5177\u6709\u6297\u83cc\u4f5c\u7528\uff0c\u5bf9\u4e34\u5e8a\u5206\u79bb\u768411 \u79cd\u81f4\u75c5\u83cc\u4f9d\u7136\u5177\u6709\u6297\u83cc\u4f5c\u7528\uff0c\u53ef\u4e3a\u4e34\u5e8a\u6297\u611f\u67d3\u6cbb\u7597\u63d0\u4f9b\u7528\u836f\u57fa\u7840\u3002\u83cc\u682a\u6765\u6e90\uff1a(1)\u8010\u5927\u73af\u5185\u916f\u7c7b\u548c\u56db\u73af\u7d20\u7c7b\u65e0\u4e73\u94fe\u7403\u83cc(\u6765\u81ea\u5206\u6ccc\u7269);(2)\u591a\u836f\u8010\u836f\u7caa\u80a0\u7403\u83ccHLA\uff32\u83cc+(\u6765\u81ea\u5c3f\u6db2);(3)\u8010\u5934\u5b62\u83cc\u7d20\u7c7b\u55dc\u9ea6\u82bd\u5be1\u517b\u5355\u80de\u83cc(\u6765\u81ea\u75f0\u6db2);(4)\u591a\u836f\u8010\u836f\u9634\u6c9f\u80a0\u6746\u83cc(\u6765\u81ea\u75f0\u6db2);(5)\u8010\u56db \u73af\u7d20\u7c7b\u805a\u56e2\u80a0\u6746\u83cc(\u6765\u81ea\u8113\u6db2);(6)\u94dc\u7eff\u5047\u5355\u80de\u83cc(\u6765\u81ea\u75f0\u6db2);(7)\u9c8d\u66fc\u4e0d\u52a8\u6746\u83cc(\u6765\u81ea\u75f0\u6db2);(8)\u591a\u836f\u8010\u836f\u5927\u80a0\u57c3\u5e0c\u83cc(\u6765\u81ea\u5c3f\u6db2);(9)\u591a\u836f\u8010\u836f\u5947\u5f02\u53d8\u5f62\u6746\u83cc(\u6765\u81ea\u5206\u6ccc\u7269);(10)\u591a\u836f\u8010\u836f\u80ba\u708e\u514b\u96f7\u4f2f\u83cc(\u6765\u81ea\u75f0\u6db2);(11)\u591a\u836f\u8010\u836f\u4ea7\u78b1\u5047\u5355\u80de\u83cc(\u6765\u81ea\u75f0\u6db2)\u3002\u4ee5\u4e0a\u83cc\u682a\u5747\u7531\u9ed1\u9f99\u6c5f\u4e2d\u533b\u836f\u5927\u5b66\u9644\u5c5e\u7b2c\u4e00\u533b\u9662\u68c0\u9a8c\u79d1\u5206\u79bb\u5e76\u9274\u5b9a\u3002<\/p>\n

2.3 \u6297\u80bf\u7624\u6d3b\u6027<\/p>\n

\u5218\u5e7f\u9050\u7b49\u7814\u7a76\u4e2d\u836f\u8fde\u7fd8\u9187\u63d0\u7269\u5bf9\u6076\u6027\u80f8\u8179\u8154\u79ef\u6db2\u4e2d\u539f\u4ee3\u80bf\u7624\u7ec6\u80de\u7684\u6297\u80bf\u7624\u4f5c\u7528\u3002\u65b9\u6cd5\uff1a\u6536\u96c625\u4f8b\u7ecf\u75c5\u7406\u786e\u8bca\u7684\u6076\u6027\u80f8\u8179\u8154\u79ef\u6db2\u5e76\u5206\u79bb\u539f\u4ee3\u80bf\u7624\u7ec6\u80de\uff0c\u7528CCK8 \u6cd5\u68c0\u6d4b\u539f\u4ee3\u80bf\u7624\u7ec6\u80de\u5bf9\u8fde\u7fd8\u9187\u63d0\u7269\u53ca\u5316\u7597\u836f\u7684\u836f\u7269\u654f\u611f\u6027\uff0c \u63d0\u53d6\u7ec6\u80deRNA\uff0c \u7528\u8367\u5149\u5b9a\u91cf\u9006\u8f6c\u5f55-\u805a\u5408\u9176\u94fe\u53cd\u5e94(
\nRT-PCR) \u6cd5\u68c0\u6d4b\u8fde\u7fd8\u9187\u63d0\u7269\u5bf9\u51cb\u4ea1\u76f8\u5173\u86cb\u767d\u57fa\u56e0SurvivinmRNA\u3001Bcl-2 mRNA\u8868\u8fbe\u7684\u4f5c\u7528\u3002\u7ed3\u679c\uff1a\u8fde\u7fd8\u9187\u63d0\u7269\u5bf94\u79cd\u7ec6\u80de\u7cfb(\u80c3\u764c\u7ec6\u80de\u682aBGC-823\u3001\u809d\u764c\u7ec6\u80de\u682aSMMC-7721\u3001\u80ba\u764c\u7ec6\u80de\u682aA549\u548c\u5927\u80a0\u764c\u7ec6\u80de\u682aLOVO )\u7684\u534a\u6570\u6291\u5236\u6d53\u5ea6( IC50<\/sub> ) \u5e73\u5747\u503c\u4e3a191.88 \u03bcg\/ml\uff0c \u5bf925\u4f8b\u6076\u6027\u80f8\u8179\u8154\u79ef\u6db2\u4e2d\u539f\u4ee3\u80bf\u7624\u7ec6\u80deIC50\u5e73\u5747\u503c\u4e3a406.13\u03bcg\/ml\uff0c \u4e24\u8005\u65e0\u663e\u8457\u5dee\u5f02( P>0.05) \u3002\u8fde\u7fd8\u9187\u63d0\u7269\u5bf9\u5927\u80a0\u764c\u3001\u80c3\u764c\u3001\u80ba\u764c\u53ca\u5176\u4ed6\u6d88\u5316\u9053\u80bf\u7624\u6765\u6e90\u7684\u80f8\u8179\u8154\u79ef\u6db2\u4e2d\u539f\u4ee3\u80bf\u7624\u7ec6\u80de\u7684\u6297\u80bf\u7624\u4f5c\u7528\u65e0\u660e\u663e\u5dee\u5f02( P>0.05) \u3002\u8fde\u7fd8\u9187\u63d0\u7269\u5bf9\u63a5\u53d7\u5316\u7597\u60a3\u8005\u7684\u80f8\u8179\u8154\u79ef\u6db2\u4e2d\u539f\u4ee3\u80bf\u7624\u7ec6\u80de\u7684IC50<\/sub>\u503c\u4e3a543.13 \u03bcg\/ml\uff0c \u663e\u8457\u9ad8\u4e8e\u672a\u63a5\u53d7\u5316\u7597\u60a3\u8005\uff0c\u5e74\u9f84\u3001\u6027\u522b\u4ee5\u53ca\u4f53\u8154\u79ef\u6db2\u7c7b\u578b\u7ec4\u95f4IC50<\/sub>\u503c\u5747\u65e0\u7edf\u8ba1\u5b66\u5dee\u5f02\u30027\u4f8b\u5bf9\u8fde\u7fd8\u9187\u63d0\u7269\u8010\u836f\u7684\u539f\u4ee3\u80bf\u7624\u7ec6\u80de\u5bf9\u591a\u897f\u7d2b\u6749\u9187\u3001\u6c1f\u5c3f\u5627\u5576\u3001\u987a\u94c2\u5b58\u5728\u4ea4\u53c9\u8010\u836f\uff0c \u800c\u5bf9\u5316\u7597\u836f\u7269\u8010\u836f\u7684\u539f\u4ee3\u80bf\u7624\u7ec6\u80de\u5bf9\u8fde\u7fd8\u9187\u63d0\u7269\u65e0\u4ea4\u53c9\u8010\u836f\u3002\u8fde\u7fd8\u9187\u63d0\u7269\u5bf9\u90e8\u5206\u7ec6\u80de\u7cfb\u53ca\u539f\u4ee3\u7ec6\u80de\u4e2d\u51cb\u4ea1\u76f8\u5173\u86cb\u767dSurvivinmRNA\u548cBcl-2 mRNA\u6709\u4e0b\u8c03\u4f5c\u7528( P<0.05) \u3002\u7ed3\u8bba\uff1a\u4e2d\u836f\u8fde\u7fd8\u9187\u63d0\u7269\u5bf9\u6076\u6027\u80f8\u8179\u8154\u79ef\u6db2\u4e2d\u539f\u4ee3\u80bf\u7624\u7ec6\u80de\uff0c\u5c24\u5176\u662f\u5bf9\u5316\u7597\u836f\u7269\u8010\u836f\u7684\u539f\u4ee3\u80bf\u7624\u7ec6\u80de\u4ecd\u6709\u4e00\u5b9a\u7684\u6297\u80bf\u7624\u4f5c\u7528\u3002<\/p>\n

\u5b59\u5a67\u7b49\u89c2\u5bdf\u8fde\u7fd8\u4e2d\u5206\u79bb\u6240\u5f97\u4e24\u79cd\u4e09\u841c\u7c7b\u5316\u5408\u7269:\u8fbe\u739b-24-\u70ef-3\u03b2-\u4e59\u9170\u6c27\u57fa-20s-\u9187[ 20 (s)-dammar-24-ene-3\u03b2\uff0c20-dio l-3\u03b2-acetate \uff0c DM] \u548c\u4e94\u73af\u9f50\u58a9\u679c\u70f7\u578b\u4e09\u841c\u7c7b\u5316\u5408\u7269\u5b89\u535a\u7acb\u9178(ambrolic acid \uff0cAA)\u5bf95 \u79cd\u4eba\u6d88\u5316\u9053\u80bf\u7624\u7ec6\u80de\u7684\u6291\u5236\u4f5c\u7528\uff0c\u4ee5\u53caAA \u5bf9\u4eba\u80c3\u764c\u7ec6\u80de\u682aSGC-7901 \u7684\u51cb\u4ea1\u8bf1\u5bfc\u4f5c\u7528\u3002\u65b9\u6cd5:MTT \u6cd5\u89c2\u5bdfAA\u548cDM \u5bf95 \u682a\u4eba\u6d88\u5316\u9053\u80bf\u7624\u7ec6\u80de\u682a\u4eba\u80c3\u764cMKN-45 \u3001\u4eba\u80c3\u764cMKN-28 \u3001\u4eba\u80c3\u764cSGC-7901 \u3001\u4eba\u80f0\u817a\u764cPNAC-1 \u3001\u4eba\u809d\u764cHepG-2 \u7684\u751f\u957f\u6291\u5236\u4f5c\u7528\uff0cA nnexin-V\/PI \u67d3\u8272\u6d41\u5f0f\u7ec6\u80de\u672f\u6d4b\u5b9aSGC-7901 \u7ec6\u80de\u7684\u51cb\u4ea1\u7387\uff0c Western blot ting \u68c0\u6d4b\u76f8\u5173\u51cb\u4ea1\u86cb\u767d\u8868\u8fbe\u3002\u7ed3\u679c:\u4e24\u79cd\u4e09\u841c\u7c7b\u5316\u5408\u7269\u5bf9\u4eba\u6d88\u5316\u9053\u80bf\u7624\u7ec6\u80de\u6709\u8f83\u597d\u7684\u6291\u5236\u4f5c\u7528\uff0c \u5176\u4f5c\u7528\u5448\u5242\u91cf\u4f9d\u8d56\u6027;AA\u4f5c\u7528\u7ec6\u80de72 h \u540e\u53ef\u4ee5\u660e\u663e\u8bf1\u5bfc\u7ec6\u80de\u51cb\u4ea1\uff0c \u800cDM\u5bf9\u7ec6\u80de\u672a\u6709\u51cb\u4ea1\u8bf1\u5bfc\u4f5c\u7528;AA \u53ef\u4e0b\u8c03procaspase3\u30016 \u30018 \u30019 \u86cb\u767d\u548cBcl-2 \u86cb\u767d\u8868\u8fbe\u6c34\u5e73\uff0c \u540c\u65f6\u53ef\u4e0a\u8c03Bax \u86cb\u767d\u6c34\u5e73\u3002\u7ed3\u8bba:AA \u548cDM \u5bf95 \u682a\u4eba\u6d88\u5316\u9053\u80bf\u7624\u7ec6\u80de\u6709\u660e\u663e\u6291\u5236\u4f5c\u7528\uff0c AA \u8bf1\u5bfcSGC-7901 \u7ec6\u80de\u51cb\u4ea1\u7684\u4e3b\u8981\u9014\u5f84\u53ef\u80fd\u4e3a\u8c03\u8282\u51cb\u4ea1\u76f8\u5173\u86cb\u767d\u7684\u8868\u8fbe\u3002<\/p>\n

\u90d1\u6631\u91c7\u7528\u56db\u7532\u57fa\u5076\u6c2e\u5511\u5170(MTT)\u6cd5\u5206\u522b\u6d4b\u5b9a\u4e86\u8fde\u7fd8\u7684\u6c34\u63d0\u6db2\u548c\u9187\u63d0\u6db2\u5bf9\u4eba\u809d\u764c SMMC-7721 \u7ec6\u80de\u682a\u4f53\u5916\u589e\u6b96\u53cd\u5e94\u7684\u5f71\u54cd\uff0c\u53d1\u73b0\u8fde\u7fd8\u9187\u63d0\u6db2\u548c\u6c34\u63d0\u6db2\u5bf9\u4eba\u809d\u764cSMMC-7721\u7ec6\u80de\u5747\u6709\u6291\u5236\u4f5c\u7528\uff0c\u4e14\u8fde\u7fd8\u9187\u63d0\u6db2\u7684\u4f5c\u7528\u8981\u660e\u663e\u4f18\u4e8e\u8fde\u7fd8\u6c34\u63d0\u6db2\uff0c\u8bc1\u660e\u4e86\u8fde\u7fd8\u9187\u63d0\u6db2\u5177\u6709\u4f53\u5916\u6291\u5236\u809d\u764c\u7ec6\u80de\u589e\u6b96\u7684\u4f5c\u7528\u3002<\/p>\n

\u80e1\u6587\u9759\u7b49\u89c2\u5bdf\u8fde\u7fd8\u4e59\u9187\u63d0\u53d6\u7269\u4f53\u5185\u3001\u4f53\u5916\u6297\u80bf\u7624\u6d3b\u6027\u3002\u65b9\u6cd5\uff1a\u4f53\u5916\u836f\u6548\u5b66\u5b9e\u9a8c\u7528MTT \u6cd5\uff0c \u4f53\u5185\u91c7\u7528\u5c0f\u9f20\u79fb\u690d\u6027\u80bf\u7624\uff0c \u89c2\u5bdf\u8fde\u7fd8\u4e59\u9187\u63d0\u53d6\u7269\u7684\u6291\u7624\u6d3b\u6027\u3002\u7ed3\u679c\uff1a\u8fde\u7fd8\u4e59\u9187\u63d0\u53d6\u7269\u4f53\u5916\u5bf9\u4eba\u809d\u764c\u7ec6\u80de\u682aSMMC-7721 \u3001\u4eba\u80a0\u764c\u7ec6\u80de\u682aLoVo \u3001\u4eba\u80c3\u4f4e\u5206\u5316\u817a\u764c\u7ec6\u80de\u682aBGC-823 \u548c\u5c0f\u9f20H22\u809d\u764c\u7ec6\u80de\u7684IC50<\/sub>\u5206\u522b\u4e3a\u5206\u522b\u4e3a1.03\u30012.40\u30011.18 \u30010 .73 mg\/ mL\u3002\u9ad8\u4f4e\u5242\u91cf\u7ec4\u4f53\u5185\u5bf9H22\u5c0f\u9f20\u809d\u764c\u6291\u7624\u7387\u5206\u522b\u4e3a53.24 %\u300135.25%\u3002\u7ed3\u8bba\uff1a\u8fde\u7fd8\u4e59\u9187\u63d0\u53d6\u7269\u4f53\u5185\u5916\u5b9e\u9a8c\u5177\u6709\u663e\u8457\u7684\u6297\u80bf\u7624\u6d3b\u6027\u3002<\/p>\n

\u5f20\u660e\u8fdc\u7b49\u63a2\u8ba8\u8fde\u7fd8\u6297\u80bf\u7624\u4f5c\u7528\u548c\u4f5c\u7528\u673a\u5236\u3002\u65b9\u6cd5: \u68c0\u6d4b\u4e86\u8fde\u7fd8\u6291\u7624\u4f5c\u7528\u53ca\u5c0f\u9f20H22\u809d\u764c\u8840\u6e05\u4e2d\u767d\u4ecb\u7d20-8( IL-8)\u548c\u80bf\u7624\u574f\u6b7b\u56e0\u5b50-\u03b1( TN F-\u03b1)\u7684\u542b\u91cf( ELISA\u6cd5)\u3002\u7ed3\u679c: \u4e0d\u540c\u5242\u91cf\u8fde\u7fd8\u80fd\u4e0d\u540c\u7a0b\u5ea6\u6291\u5236\u5c0f\u9f20H22\u809d\u764c\u7684\u751f\u957f\uff0c\u80fd\u589e\u52a0TN F-\u03b1\u548c\u964d\u4f4eIL-8\u7684\u542b\u91cf\u3002\u7ed3\u8bba: \u8fde\u7fd8\u5bf9\u5c0f\u9f20H22\u809d\u764c\u5177\u6709\u6291\u5236\u4f5c\u7528\u3002<\/p>\n

\u8d75\u6668\u9633\u7b49\u5b9e\u9a8c\u53d1\u73b0\u8fde\u7fd8\u8102\u7d20\u5bf9\u4eba\u809d\u764c\u7ec6\u80de\u3001\u4eba\u5bab\u9888\u764c\u7ec6\u80de\u3001\u4e2d\u56fd\u4ed3\u9f20\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u5177\u6709\u663e\u8457\u7684\u4f53\u5916\u6297\u80bf\u7624\u6d3b\u6027\uff0c\u76f8\u5bf9\u4e8e\u957f\u6625\u65b0\u78b1\u8fde\u7fd8\u8102\u7d20\u5bf9\u5c0f\u9f20\u9ed1\u8272\u7d20\u8089\u7624\u7ec6\u80deB16 \u7684\u6291\u5236\u751f\u957f\u4f5c\u7528\u66f4\u5f3a\u3002<\/p>\n

\u5b59\u58eb\u840d\u7b49\u91c7\u7528MTT \u6cd5\u68c0\u6d4b\u8fde\u7fd8\u6839\u9187\u63d0\u7269\u5bf9\u98df\u7ba1\u764cTE-1\u3001TE-13\u3001Yes-2 \u548cEca-109 \u7ec6\u80de\u589e\u6b96\u7684\u6291\u5236\u4f5c\u7528\uff0c\u7ed3\u679c\u53d1\u73b0\uff0c\u6cbb\u7597\u7ec4\u88f8\u9f20\u80bf\u7624\u7ec6\u80de\u51cb\u4ea1\u6570\u91cf\u660e\u663e\u9ad8\u4e8e\u5bf9\u7167\u7ec4\uff0cBcl-2 \u86cb\u767d\u7684\u8868\u8fbe\u6c34\u5e73\u4f4e\u4e8e\u5bf9\u7167\u7ec4\uff0cBax \u86cb\u767d\u8868\u8fbe\u6c34\u5e73\u9ad8\u4e8e\u5bf9\u7167\u7ec4\u3002\u8fde\u7fd8\u6839\u9187\u63d0\u7269\u901a\u8fc7\u8bf1\u5bfc\u98df\u7ba1\u764c\u7ec6\u80de\u51cb\u4ea1\uff0c\u8fbe\u5230\u5bf9\u98df\u7ba1\u764c\u7ec6\u80de\u7684\u751f\u957f\u6291\u5236\u4f5c\u7528\u3002<\/p>\n

\u989c\u6670\u7b49\u989c\u6670\u7b49\u5206\u522b\u5bf9\u8fde\u7fd8\u6839\u3001\u53f6\u548c\u679c3\u4e2a\u90e8\u4f4d\u8fdb\u884c\u6c34\u548c\u4e59\u9187\u7684\u63d0\u7eaf\uff0c\u5f97\u5230\u76f8\u5e94\u7684\u63d0\u53d6\u7269\u3002\u7136\u540e\u9488\u5bf96 \u79cd\u8fde\u7fd8\u63d0\u53d6\u7269\u5bf9\u98df\u7ba1\u764c\u7ec6\u80deTE-13\u3001TE-1\u3001YES-2\u3001Eca-109\u7684\u589e\u6b96\u6291\u5236\u4f5c\u7528\u9009\u7528MTT\u6cd5\u8fdb\u884c\u89c2\u5bdf\u68c0\u6d4b\uff1b\u4e4b\u540e\u5bf9\u98df\u9053\u764c\u7ec6\u80de\u91c7\u7528\u4e0d\u540c\u6d53\u5ea6FSEER \u5904\u740624 h \u540e\u7528\u5012\u7f6e\u76f8\u5dee\u663e\u5fae\u955c\u548c\u745e\u6c0f- \u5409\u59c6\u8428\u67d3\u8272\u89c2\u5bdf\u7ec6\u80de\u7684\u5f62\u6001\u53d8\u5316\uff0c\u518d\u4f9d\u6b21\u7528\u9009\u5b9a\u7684\u5404\u4e2a\u6d53\u5ea6\u7684FSEER\u5bf9TE-13 \u7ec6\u80de\u8fdb\u884c\u4e0d\u540c\u65f6\u95f4\u7684\u5904\u7406\u540e\uff0c\u5bf9TE-13 \u7ec6\u80de\u51cb\u4ea1\u7387\u7684\u53d8\u5316\u4ee5Annexin V\/PI \u53cc\u67d3\u6cd5\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u4eea\u8fdb\u884c\u68c0\u6d4b\uff0c\u914d\u5408westeron blot \u6cd5\u89c2\u5bdfPARP\u86cb\u767d\u8868\u8fbe\u53d8\u5316\u3002\u53d1\u73b0\u5728\u98df\u7ba1\u4e2d6\u79cd\u8fde\u7fd8\u63d0\u53d6\u7269\u5bf9\u98df\u7ba1\u764cTE-13 \u7ec6\u80de\u5747\u6709\u4e0d\u540c\u7a0b\u5ea6\u7684\u589e\u6b96\u6291\u5236\u4f5c\u7528\u3002\u7ecfFSEER \u5904\u7406\u540e\uff0cTE-13 \u7ec6\u80de\u8868\u73b0\u51fa\u5178\u578b\u7684\u51cb\u4ea1\u5f62\u6001\u53d8\u5316\u3002<\/p>\n

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\u8c22\u4e3d\u7b49\u91c7\u7528 MTT \u6cd5\u68c0\u6d4b\u8fde\u7fd8\u7684\u9187\u63d0\u7269 DM \u5bf9\u80c3\u764c\u7ec6\u80de\u682a SGC-7901\u3001BGC-823 \u548c MKN-45 \u4f53\u5916\u6291\u5236\u4f5c\u7528\uff0c\u5e76\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u4eea\u68c0\u6d4b\u8be5\u63d0\u53d6\u7269\u5bf9\u7ec6\u80de\u51cb\u4ea1\u60c5\u51b5\u7684\u5f71\u54cd\uff0c\u7ed3\u679c\u53d1\u73b0 DM \u5bf9\u4e8e 3\u682a\u80c3\u764c\u7ec6\u80de\u6291\u5236\u7387\u5747\u5728 80%\u4ee5\u4e0a\uff0c\u800c\u4e14 DM \u80fd\u591f\u5c06\u80c3\u764c\u7ec6\u80de\u5468\u671f\u963b\u6ede\u4e8e S \u671f\uff0c\u8bf1\u5bfc\u7ec6\u80de\u51cb\u4ea1\uff0c\u4ece\u800c\u6291\u5236\u7ec6\u80de\u589e\u6b96\u4f5c\u7528\u3002<\/p>\n

\u5218\u5e7f\u9050\u7b49\u7814\u7a76\u6c49\u9632\u5df1\u7532\u7d20\u3001\u85e4\u9ec4\u9178\u3001\u91cd\u697c\u9187\u63d0\u7269\u3001\u8fde\u7fd8\u9187\u63d0\u7269\u5bf9\u591a\u897f\u7d2b\u6749\u9187\u8010\u836f\u80c3\u764c\u7ec6\u80de\u7684\u4f5c\u7528\uff0c\u8fdb\u4e00\u6b65\u63a2\u8ba8\u4e2d\u836f\u7684\u4f5c\u7528\u673a\u7406\u3002\u65b9\u6cd5: \u4f53\u5916\u5efa\u7acb\u591a\u897f\u7d2b\u6749\u9187\u8010\u836f\u7684\u80c3\u764cBGC- 823 \u7ec6\u80de\u682a\uff0c MTT \u6cd5\u68c0\u6d4b\u7ec6\u80de\u7684\u8010\u836f\u6307\u6570\u53ca\u4e2d\u836f\u5bf9\u4eb2\u672c\u548c\u8010\u836f\u7ec6\u80de\u7684\u7ec6\u80de\u6bd2\u4f5c\u7528\u3002\u7ed3\u679c: \u7ecf\u8fc7\u4f53\u5916\u8bf1\u5bfc\u5efa\u7acb\u7684BGC- 823\/Doc \u8010\u836f\u7ec6\u80de\u682a\uff0c \u5bf9\u591a\u897f\u7d2b\u6749\u9187\u8010\u836f\u6307\u6570\u4e3a45.7\uff0c\u5bf9\u7d2b\u6749\u9187\u8010\u836f\u6307\u6570\u4e3a33.5\uff0c \u4e8c\u8005\u6709\u90e8\u5206\u4ea4\u53c9\u8010\u836f\uff1b\u5bf9\u6c49\u9632\u5df1\u7532\u7d20\u7684\u8010\u836f\u6307\u6570\u4e3a0.93\uff0c\u5bf9\u91cd\u697c\u9187\u63d0\u7269\u7684\u8010\u836f\u6307\u6570\u4e3a1.03\uff0c \u5bf9\u8fde\u7fd8\u9187\u63d0\u7269\u7684\u8010\u836f\u6307\u6570\u4e3a1.20\uff0c \u5bf9\u85e4\u9ec4\u9178\u7684\u8010\u836f\u6307\u6570\u4e3a0.95\u3002BGC- 823\/Doc \u5bf9\u7d2b\u6749\u9187\u3001\u8fde\u7fd8\u9187\u63d0\u7269\u6709\u90e8\u5206\u4ea4\u53c9\u8010\u836f\uff0c \u5bf9\u6c49\u9632\u5df1\u7532\u7d20\u3001\u91cd\u697c\u9187\u63d0\u7269\u3001\u85e4\u9ec4\u9178\u65e0\u4ea4\u53c9\u8010\u836f\u3002\u6c49\u9632\u5df1\u7532\u7d20\u3001\u91cd\u697c\u9187\u63d0\u7269\u3001\u85e4\u9ec4\u9178\u7684\u7ec6\u80de\u6bd2\u4f5c\u7528\u65e0\u7edf\u8ba1\u5b66\u5dee\u5f02\u3002\u7ed3\u8bba: \u6c49\u9632\u5df1\u7532\u7d20\u3001\u91cd\u697c\u3001\u85e4\u9ec4\u9178\u5bf9BGC- 823\/Doc \u8010\u836f\u7ec6\u80de\u682a\u6709\u7ec6\u80de\u6bd2\u4f5c\u7528\uff0c\u4e3a\u4e2d\u836f\u5728\u591a\u897f\u7d2b\u6749\u9187\u8010\u836f\u60a3\u8005\u4e2d\u7684\u5e94\u7528\u5960\u5b9a\u4e86\u7406\u8bba\u57fa\u7840\u3002<\/p>\n

\u6bb7\u6d77\u6d9b\u7b49\u89c2\u5bdf\u8fde\u7fd8\u4e2d\u5206\u79bb\u6240\u5f97\u4e09\u841c\u7c7b\u5316\u5408\u7269\u8fbe\u739b-24-\u70ef-3\u03b2-\u4e59\u9170\u6c27\u57fa-20S-\u9187(Dammar-24-ene-3\u03b2-acetate-20S-ol\uff0cDM)\u5bf9\u4eba\u4f4e\u5206\u5316\u80c3\u764c\u7ec6\u80deBGC-823\u7684\u589e\u6b96\u6291\u5236\u4f5c\u7528\u53caDM \u7684\u653e\u7597\u589e\u654f\u4f5c\u7528\u3002\u65b9\u6cd5\uff1a\u6d41\u5f0f\u7ec6\u80de\u4eea\u68c0\u6d4b\u4e0d\u540c\u6d53\u5ea6DM \u6db2\u5bf9BGC-823\u7ec6\u80de\u5468\u671f\u3001\u51cb\u4ea1\u7684\u5f71\u54cd\uff1b\u514b\u9686\u5f62\u6210\u6cd5\u6d4b\u5b9a\u7ec6\u80de\u5b58\u6d3b\u5206\u6570\uff1b\u8ba1\u7b97\u5404\u9879\u653e\u5c04\u5b66\u53c2\u6570Do\u3001Dq\u3001N\u53ca\u653e\u7597\u589e\u654f\u6bd4\u3002\u7ed3\u679c\uff1aDM \u53ef\u4ee5\u8bf1\u5bfcBGC-823\u7ec6\u80de\u51cb\u4ea1\uff0c\u5f15\u8d77\u7ec6\u80de\u5468\u671f\u4e2dG1\u671f\u6bd4\u4f8b\u589e\u9ad8\uff0c\u800cS\u671f\u6bd4\u4f8b\u964d\u4f4e\u300225\u03bcg\uff0fmL DM \u6db2\u4f5c\u7528BGC-823\u7ec6\u80de48 h\u663e\u793a\u51fa\u6709\u653e\u7597\u589e\u654f\u4f5c\u7528\uff0c\u653e\u7597\u589e\u654f\u6bd4\u8fbe1\uff0e97\u3002\u7ed3\u8bba\uff1aDM\u53ef\u4ee5\u5f15\u8d77BGC-823\u7ec6\u80de\u51cb\u4ea1\uff0cDM \u6709\u653e\u7597\u589e\u654f\u4f5c\u7528\u3002<\/p>\n

\u8bb8\u840d\u7b49\u63a2\u8ba8\u8fde\u7fd8\u4e59\u9187\u63d0\u53d6\u7269(EEFF)\u5bf9\u4eba\u4f4e\u5206\u5316\u80c3\u817a\u764c\u7ec6\u80de\u682aBGC-823\u4f53\u5916\u589e\u6b96\u548c\u51cb\u4ea1\u7684\u5f71\u54cd\u3002\u65b9\u6cd5:\u56db\u7532\u57fa\u5076\u6c2e\u5511\u76d0(MTT)\u6bd4\u8272\u6cd5\u6d4b\u4e0d\u540cEEFF\u6d53\u5ea6(31.25\u300162.5\u3001125\u3001250\u3001500\u548c1 000\u03bcg\/ml)\u5904\u740624\u300148\u548c72h\u5bf9BGC-823\u7ec6\u80de\u589e\u6b96\u7684\u6291\u5236\u7387\u3002AnnexinV\/PI\u53cc\u67d3\u8272\u6cd5\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u4eea\u68c0\u6d4b\u4e0d\u540c\u6d53\u5ea6EEFF(0\u3001200\u548c400\u03bcg\/ml)\u5904\u740624h\u53ca400\u03bcg\/mlEEFF\u5206\u522b\u5904\u740624\u548c48h\u7684BGC-823\u7ec6\u80de\u51cb\u4ea1\u7387\u3002\u3000\u7ed3\u679c:EEFF\u4f5c\u7528\u4e8eBGC-823\u7ec6\u80de24\u300148\u548c72h\u7684\u534a\u6570\u6291\u5236\u6d53\u5ea6(IC50 )\u5206\u522b\u4e3a(566.21 \u00b131.76)\u3001(338.91 \u00b129.11)\u548c(159.01 \u00b117.23)\u03bcg\/ ml\uff0c\u4e14\u5176\u4f5c\u7528\u5448\u660e\u663e\u7684\u65f6\u6548\u548c\u5242\u91cf\u5173\u7cfb\u3002\u4e0d\u540c\u6d53\u5ea6EEFF(0\u3001200\u548c400\u03bcg\/ml)\u5904\u740624 h\u7684BGC-823 \u7ec6\u80de\u51cb\u4ea1\u7387\u5206\u522b\u4e3a8.3%\u300110.4%\u548c16.4%;400\u03bcg\/mlEEFF\u5206\u522b\u5904\u7406BGC-823\u7ec6\u80de24 \u548c48 h\u7684\u7ec6\u80de\u51cb\u4ea1\u7387\u5206\u522b\u4e3a18.7%\u548c33.3%\u3002\u7ed3\u8bba:EEFF\u5bf9BGC-823\u7ec6\u80de\u6709\u6291\u5236\u589e\u6b96\u548c\u4fc3\u51cb\u4ea1\u4f5c\u7528\u3002<\/p>\n

\u90ed\u4e1c\u5317\u7b49\u89c2\u5bdf\u8fde\u7fd8\u63d0\u53d6\u7269LQ-4\u5bf9SGC-7901 \u80c3\u764c\u7ec6\u80de\u4f53\u5916\u4fc3\u51cb\u4ea1\u4f5c\u7528\uff0c\u4e3aLQ-4 \u5728\u6297\u80bf\u7624\u673a\u5236\u7814\u7a76\u53ca\u5e94\u7528\u63d0\u4f9b\u7406\u8bba\u4f9d\u636e\u3002\u65b9\u6cd5\u5e94\u7528MTT \u6cd5\u6d4b\u5b9aLQ-4\u5bf9SGC-7901 \u80c3\u764c\u7ec6\u80de\u589e\u6b96\u7684\u6291\u5236\u4f5c\u7528; \u91c7\u7528AO-EB \u67d3\u8272\u3001\u900f\u5c04\u7535\u955c\u3001\u6d41\u5f0f\u7ec6\u80de\u4eea\u68c0\u6d4b\u5176\u4fc3\u51cb\u4ea1\u4f5c\u7528\u3002\u7ed3\u679cLQ-4 \u5bf9SGC-7901 \u80c3\u764c\u7ec6\u80de\u4f53\u5916\u589e\u6b96\u6291\u5236\u5448\u660e\u663e\u7684\u5242\u91cf\u65f6\u95f4\u4f9d\u8d56\u6027\uff0cLQ-4 \u5904\u7406\u7ec6\u80de6\u300112\u300124 h \u540e\u7684\u534a\u6570\u6291\u5236\u6d53\u5ea6( IC50) \u5206\u522b\u4e3a( 73. 27 \u00b1 3. 19) \u03bcg \/ml\u3001( 44. 63 \u00b1 2. 06) \u03bcg \/ml\u3001( 35. 99 \u00b1 2. 43) \u03bcg \/ml\uff0c\u5404\u7ec4\u95f4\u5dee\u5f02\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\uff0cF= 15. 25( P \uff1c 0. 01) ; AO-EB \u67d3\u8272\u8868\u660e\u836f\u7269\u7ec4\u7ec6\u80de\u51fa\u73b0\u660e\u663e\u51cb\u4ea1\uff0c\u9634\u6027\u5bf9\u7167\u7ec4\u7ec6\u80de\u5f62\u6001\u5b8c\u597d; \u900f\u5c04\u7535\u955c\u8868\u660e\uff0cSGC-7901 \u80c3\u764c\u7ec6\u80de\u5728LQ-4 \u5904\u7406\u540e\u51fa\u73b0\u5178\u578b\u51cb\u4ea1\u7ec6\u80de\u5f62\u6001\u6539\u53d8; FCM \u5206\u6790\u7ed3\u679c\u8868\u660e\uff0cSGC-7901\u80c3\u764c\u7ec6\u80de\u5728LQ-4 \u5904\u7406\u524d\u540e\u7ec6\u80de\u51cb\u4ea1\u7387\u53d1\u751f\u6539\u53d8\uff0c\u5176\u4e2d\u6eb6\u5a92\u5bf9\u7167\u7ec4\u4e3a( 0. 73 \u00b1 0. 49) %\uff0c25\u300150\u3001100 \u03bcg \/ml LQ-4 \u4f5c\u7528\u7ec4\u5206\u522b\u4e3a( 8. 04 \u00b1 0. 68) %\u3001( 18. 45 \u00b1 0. 83) %\u3001( 52. 37 \u00b1 0. 74) %\uff0c\u5404\u7ec4\u95f4\u5dee\u5f02\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\uff0cF = 13. 17( P \uff1c 0. 05) \u3002\u7ed3\u8bba\u8fde\u7fd8\u63d0\u53d6\u7269LQ-4 \u4f53\u5916\u80fd\u660e\u663e\u6291\u5236SGC-7901 \u80c3\u764c\u7ec6\u80de\u589e\u6b96\u5e76\u8bf1\u5bfc\u5176\u53d1\u751f\u51cb\u4ea1\uff0c\u5177\u6709\u660e\u663e\u7684\u6297\u80bf\u7624\u4f5c\u7528\u3002<\/p>\n

\u9648\u747e\u672c\u5b9e\u9a8c\u5206\u522b\u8bbe\u7acb0%\uff0c5%\uff0c20%\uff0c35%\uff0c50%\uff0c65%\uff0c80%\uff0c95%\u4e59\u9187 8 \u4e2a\u6d53\u5ea6\uff0c\u4ee5\u5bf9\u4eba\u4e73\u817a\u764c MCF-7 \u7ec6\u80de\u548c MDA-MB-231 \u7ec6\u80de\u589e\u503c\u7684\u6291\u5236\u4f5c\u7528\u4e3a\u6307\u5bfc\uff0c\u4f18\u9009\u8fde\u7fd8\u5f52\u5c3e\u714e\uff08\u8fde\u7fd8\u3001\u91d1\u94f6\u82b1\u3001\u7518\u8349\u3001\u5f52\u5c3e\u3001\u5927\u8840\u85e4\uff09\u6700\u4f73\u7684\u63d0\u53d6\u6eb6\u5a92\uff1b\u901a\u8fc7 HPLC \u6cd5\u6d4b\u5b9a\u8fde\u7fd8\u5f52\u5c3e\u714e\u4e0d\u540c\u4e59\u9187\u6d53\u5ea6\u63d0\u53d6\u7269\u4e2d\u6709\u6548\u6210\u5206\u7684\u542b\u91cf\uff0c\u52a0\u6743\u8ba1\u7b97\u6709\u6548\u6210\u5206\u603b\u7684\u8f6c\u79fb\u7387\uff0c\u786e\u5b9a\u6700\u4f73\u7684\u63d0\u53d6\u6eb6\u5a92\u3002\u5c06\u8fde\u7fd8\u5f52\u5c3e\u714e\u4e59\u9187\u63d0\u53d6\u7269\u7a00\u91ca\u6210\u4e0d\u540c\u7684\u836f\u7269\u6d53\u5ea6\uff0c\u8bbe\u7acb\u7a7a\u767d\u7ec4\u3001\u9634\u6027\u5bf9\u7167\u7ec4\u3001\u9633\u6027\u5bf9\u7167\u7ec4\u3001\u52a0\u836f\u7ec4\uff0c\u6d4b\u5b9a\u836f\u7269\u4f5c\u7528 24h\u300148h\u300172h \u540e\uff0c\u4e0d\u540c\u7ed9\u836f\u6d53\u5ea6\u8fde\u7fd8\u5f52\u5c3e\u714e\u4e59\u9187\u63d0\u53d6\u7269\u5bf9\u4e73\u817a\u764c\u7ec6\u80de\u589e\u503c\u7684\u6291\u5236\u4f5c\u7528\uff0c\u6c42\u51fa IC50\uff1b\u901a\u8fc7\u7ec6\u80de\u5212\u75d5\u5b9e\u9a8c\u7814\u7a76\u8fde\u7fd8\u5f52\u5c3e\u714e\u4e59\u9187\u63d0\u53d6\u7269\u5bf9\u7ec6\u80de\u8fc1\u79fb\u80fd\u529b\u7684\u5f71\u54cd\uff1b\u91c7\u7528 Annexin V\/PI \u53cc\u67d3\u8272\u6cd5\u68c0\u6d4b\u8fde\u7fd8\u5f52\u5c3e\u714e\u4e59\u9187\u63d0\u53d6\u7269\u5bf9\u7ec6\u80de\u51cb\u4ea1\u7684\u5f71\u54cd\uff1bPI \u5355\u67d3\u8272\u6cd5\u68c0\u6d4b\u8fde\u7fd8\u5f52\u5c3e\u714e\u4e59\u9187\u63d0\u53d6\u7269\u5bf9\u7ec6\u80de\u5468\u671f\u7684\u5f71\u54cd\u3002\u7ed3\u679c\uff1a\u4f53\u5916\u7ec6\u80de\u589e\u503c\u6291\u5236\u5b9e\u9a8c\u548c\u6709\u6548\u6210\u5206\u8f6c\u79fb\u7387\u7684\u6d4b\u5b9a\u7ed3\u679c\uff0c8 \u4e2a\u4e59\u9187\u63d0\u53d6\u6d53\u5ea6\u4e2d\uff0c\u7efc\u5408\u6548\u679c\u6700\u597d\u7684\u662f 20%\u4e59\u9187\u63d0\u53d6\u7269\u3002\u5728\u4f5c\u7528 24h\u300148h\u300172h \u540e\uff0c20%\u4e59\u9187\u63d0\u53d6\u7269\u5bf9\u4e8eMDA-MB-231 \u7ec6\u80de\u589e\u503c\u6291\u5236\u7387\u5206\u522b\u4e3a 65.05%\u300178.03%\u300187.84%\uff0c\u5bf9\u4e8e MCF-7 \u7ec6\u80de\u589e\u503c\u6291\u5236\u7387\u5206\u522b\u4e3a 70.61%\u300173.92%\u300175.20%\uff0c\u6709\u6548\u6210\u5206\u7684\u8f6c\u79fb\u7387\u4e3a 46.25%\u3002\u8fde\u7fd8\u5f52\u5c3e\u714e\u4e59\u9187\u63d0\u7269\u5bf9\u4e73\u817a\u764c\u7ec6\u80de\u4f53\u5916\u836f\u6548\u5b9e\u9a8c\u7814\u7a76\uff1a\u5b9e\u9a8c\u53d1\u73b0\u8fde\u7fd8\u5f52\u5c3e\u714e\u4e59\u9187\u63d0\u53d6\u7269\u5bf9\u4eba\u4e73\u817a\u764c MDA-MB-231 \u7ec6\u80de\u548c MCF-7\u7ec6\u80de\u7684\u6291\u5236\u4f5c\u7528\u968f\u4f5c\u7528\u65f6\u95f4\u8d8a\u957f\uff0c\u6291\u5236\u4f5c\u7528\u8d8a\u5927\uff0c\u836f\u7269\u6d53\u5ea6\u8d8a\u5927\uff0c\u6291\u5236\u4f5c\u7528\u8d8a\u5927\uff0c\u901a\u8fc7\u8ba1\u7b97\uff0c\u4f5c\u7528\u65f6\u95f4 24h\u300148h\u300172h \u540e\uff0cMDA-MB-231 \u7ec6\u80de IC50<\/sub> \u5206\u522b\u4e3a 0.7959mg\/mL\u30010.6989mg\/mL\u30010.5107mg\/mL\uff0cMCF-7 \u7684 IC50 \u5206\u522b\u4e3a 0.5642mg\/mL\u30010.5576mg\/mL\u30010.5335mg\/mL\uff0cIC50 \u6d53\u5ea6\u968f\u7740\u4f5c\u7528\u65f6\u95f4\u7684\u589e\u957f\u53d8\u5c0f\uff0cP<0.05\uff0c\u6570\u636e\u5177\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\u3002\u5bf9\u4e8e MDA-MB-231 \u7ec6\u80de\uff0c\u7ec6\u80de\u7684\u8fc1\u79fb\u7387\u4e3a 67.26%\uff0c\u52a0\u836f\u540e\uff0c\u4f4e\u5242\u91cf\u7ec4\u3001\u4e2d\u5242\u91cf\u7ec4\u3001\u9ad8\u5242\u91cf\u7ec4\u7ec6\u80de\u7684\u8fc1\u79fb\u7387\u5206\u522b\u4e3a15.96%\u300114.47%\u30016.78%\uff0c\u5bf9\u4e8e MCF-7 \u7ec6\u80de\uff0c\u7ec6\u80de\u7684\u8fc1\u79fb\u7387\u4e3a 36.73%\uff0c\u52a0\u836f\u540e\uff0c\u4f4e\u5242\u91cf\u7ec4\u3001\u4e2d\u5242\u91cf\u7ec4\u3001\u9ad8\u5242\u91cf\u7ec4\u7ec6\u80de\u7684\u8fc1\u79fb\u7387\u5206\u522b\u4e3a8.56%\u30013.66%\u3001-1.65%\uff0c\u8bf4\u660e\u8fde\u7fd8\u5f52\u5c3e\u714e\u4f1a\u5f71\u54cd\u7ec6\u80de\u7684\u8fc1\u79fb\u80fd\u529b\uff0c\u836f\u7269\u6d53\u5ea6\u8d8a\u5927\uff0c\u5f71\u54cd\u80fd\u529b\u8d8a\u5927\uff0cP<0.05\uff0c\u6570\u636e\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\u3002\u5bf9\u4e8e MDA-MB-231 \u7ec6\u80de\uff0c\u8fde\u7fd8\u5f52\u5c3e\u714e\u6d53\u5ea6\u5206\u522b\u4e3a 0.5mg\/mL\u30010.7mg\/mL\u30010.9mg\/mL\uff0c\u4f5c\u7528 12h\uff0c\u51cb\u4ea1\u7387\u5206\u522b\u4e3a 10.88%\u300116.85%\u300117.92%\uff0c\u4f5c\u7528 24h\uff0c\u51cb\u4ea1\u7387\u5206\u522b\u4e3a 13.08%\u300117.9%\u300128.51%\uff0c\u4f5c\u7528 48h\uff0c\u51cb\u4ea1\u7387\u5206\u522b\u4e3a 31.16%\u300135.94%\u300137.04%\uff1b\u5bf9\u4e8e MCF-7 \u7ec6\u80de\uff0c\u8fde\u7fd8\u5f52\u5c3e\u714e\u6d53\u5ea6\u5206\u522b\u4e3a 0.3mg\/mL\u30010.5mg\/mL\u30010.7mg\/mL\uff0c\u4f5c\u752812h\uff0c\u51cb\u4ea1\u7387\u5206\u522b\u4e3a 16.33%\u300119.84%\u300120.52%\uff0c\u4f5c\u7528 24h\uff0c\u51cb\u4ea1\u7387\u5206\u522b\u4e3a 22.78%\u300132.63%\u300137.33%\uff0c\u4f5c\u7528 48h\uff0c\u51cb\u4ea1\u7387\u5206\u522b\u4e3a 29.31%\u300136.52%\u300142.73%\uff1b\u8fde\u7fd8\u5f52\u5c3e\u714e\u4f5c\u7528\u4e8e\u4eba\u4e73\u817a\u764c MDA-MB-231 \u7ec6\u80de\u548c MCF-7 \u7ec6\u80de\uff0c\u4f1a\u4e0d\u540c\u7a0b\u5ea6\u7684\u4fc3\u8fdb\u7ec6\u80de\u7684\u51cb\u4ea1\uff0c\u51cb\u4ea1\u7387\u968f\u4f5c\u7528\u65f6\u95f4\u589e\u957f\u3001\u836f\u7269\u6d53\u5ea6\u589e\u5927\u800c\u589e\u9ad8\uff0cP<0.05\uff0c\u6570\u636e\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\u3002\u5bf9\u4e8e MDA-MB-231 \u7ec6\u80de\uff0c\u9633\u6027\u5bf9\u7167\u7ec4\u5728\u52a0\u836f 24h \u65f6\uff0c\u901a\u8fc7\u589e\u5927\u7ec6\u80de G0 \/ G1\u671f\u6bd4\u4f8b\uff0c\u6291\u5236\u7ec6\u80de\u7684\u589e\u6b96\uff0c\u968f\u7740\u4f5c\u7528\u65f6\u95f4\u589e\u957f\uff0c\u5219\u901a\u8fc7\u589e\u5927 G2 \/ M \u671f\u6765\u6291\u5236\u7ec6\u80de\u7684\u589e\u6b96\uff1b\u5728\u52a0\u836f 24h \u65f6\uff0c\u8fde\u7fd8\u5f52\u5c3e\u714e\u4fc3\u8fdb\u7ec6\u80de\u7684\u589e\u6b96\uff0c\u4f46\u968f\u7740\u4f5c\u7528\u65f6\u95f4\u589e\u957f\uff0c\u901a\u8fc7\u589e\u5927G0 \/ G1 \u671f\u6bd4\u4f8b\u589e\u5927\uff0c\u6291\u5236\u7ec6\u80de\u7684\u589e\u6b96\uff1b\u5bf9\u4e8e MCF-7 \u7ec6\u80de\uff0c\u9633\u6027\u5bf9\u7167\u7ec4\u3001\u8fde\u7fd8\u5f52\u5c3e\u714e\u52a0\u836f 24h\u300148h\u300172h \u90fd\u662f\u901a\u8fc7\u589e\u5927\u7ec6\u80de G0 \/ G1 \u671f\u6bd4\u4f8b\uff0c\u6291\u5236\u7ec6\u80de\u7684\u589e\u6b96\uff0c\u968f\u836f\u7269\u6d53\u5ea6\u8d8a\u5927\uff0c\u4f5c\u7528\u65f6\u95f4\u589e\u957f\uff0c\u6291\u5236\u4f5c\u7528\u8d8a\u5f3a\uff0cP<0.05\uff0c\u6570\u636e\u5177\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\u3002\u7ed3\u8bba\uff1a\u7fd8\u5f52\u5c3e\u714e\u80fd\u660e\u663e\u6291\u5236\u4eba\u4e73\u817a\u764c MDA-MB-231 \u7ec6\u80de\u548c MCF-7 \u7ec6\u80de\u7684\u589e\u503c\uff0c\u5177\u6709\u91cf-\u6548\u3001\u65f6-\u6548\u5173\u7cfb\uff1b\u5f71\u54cd\u7ec6\u80de\u7684\u8fc1\u79fb\u80fd\u529b\uff0c\u4fc3\u8fdb\u7ec6\u80de\u7684\u51cb\u4ea1\uff0c\u589e\u5927\u7ec6\u80de G0 \/
\nG1\u671f\u6bd4\u4f8b\uff0c\u4ece\u800c\u6291\u5236\u7ec6\u80de DNA \u7684\u5408\u6210\u3002<\/p>\n

\u66f2\u6b23\u7b49\u63a2\u8ba8\u8fde\u7fd8\u6297\u80bf\u7624\u6d3b\u6027\u6210\u5206LQ-4 \u4f53\u5916\u5bf9\u4eba\u5bab\u9888\u764cHeLa \u7ec6\u80de\u7684\u589e\u6b96\u6291\u5236\u548c\u8bf1\u5bfcHeLa \u7ec6\u80de\u51cb\u4ea1\u53ca\u673a\u5236\u3002\u65b9\u6cd5\u901a\u8fc7\u6c34\u63d0\u53d6\u3001\u9187\u6c89\u3001\u5927\u5b54\u5438\u9644\u6811\u8102\u67f1\u5c42\u6790\u5206\u79bb\u3001\u8584\u5c42\u5c42\u6790\uff0c\u5206\u79bb\u63d0\u53d6LQ-4; \u56db\u7532\u57fa\u5076\u6c2e\u5511\u84dd\u6bd4\u8272\u6cd5\u89c2\u5bdfLQ-4 \u5bf9HeLa \u7ec6\u80de\u589e\u6b96\u6291\u5236\u4f5c\u7528; \u5416\u5576\u6a59\/\u6eb4\u5316\u4e59\u952d\u53cc\u8367\u5149\u67d3\u8272\u6cd5\u3001\u900f\u5c04\u7535\u955c\u89c2\u5bdf\u7ec6\u80de\u51cb\u4ea1\u7684\u5f62\u6001\u5b66\u53d8\u5316;
\nwestern blot \u6cd5\u6d4b\u5b9aHeLa\u7ec6\u80decaspase-8 \u8868\u8fbe\u6c34\u5e73\u3002\u7ed3\u679cLQ-4 \u4f53\u5916\u5bf9HeLa \u7ec6\u80de\u7684\u751f\u957f\u3001\u589e\u6b96\u5747\u6709\u6291\u5236\u4f5c\u7528\uff0c 12\u300124 \u548c48 h \u7684\u534a\u6570\u6291\u5236\u6d53\u5ea6( IC50<\/sub>) \u5206\u522b\u4e3a93. 74\u300133. 30\u300122. 65 \u03bcg \/mL; LQ-4 \u4f5c\u7528HeLa \u7ec6\u80de\u540e\uff0c\u900f\u5c04\u7535\u955c\u89c2\u5bdf\u53ef\u89c1\u5178\u578b\u51cb\u4ea1\u7ec6\u80de\u5f62\u6001\uff1bLQ-4 \u4f5c\u7528\u4e8eHeLa \u7ec6\u80de\u540e\uff0c\u4fc3\u8fdbcaspase-8 \u9176\u539f\u6d3b\u5316\uff0c\u572843 \u53ca18 kD \u4e0a\u5747\u6709\u8868\u8fbe\u3002\u7ed3\u8bbaLQ-4 \u5bf9HeLa\u7ec6\u80de\u7684\u589e\u6b96\u5177\u6709\u6291\u5236\u4f5c\u7528\uff0c\u53ef\u8bf1\u5bfcHeLa \u7ec6\u80de\u51cb\u4ea1\uff0c\u5176\u673a\u5236\u53ef\u80fd\u4e0ecaspase-8 \u86cb\u767d\u9176\u539f\u88c2\u89e3\u6709\u5173\u3002<\/p>\n

\u8521\u670b\u670b\u7b49\u63a2\u7d22\u8fde\u7fd8\u63d0\u53d6\u7269LQ-4\u4f53\u5916\u8bf1\u5bfc\u5bab\u9888\u764cHela \u7ec6\u80de\u51cb\u4ea1\u4f5c\u7528\u3002\u65b9\u6cd5\uff1a\u5e94\u7528MTT \u6cd5\u6d4b\u5b9aLQ-4\u5bf9\u5bab\u9888\u764cHela \u7ec6\u80de\u7684\u589e\u6b96\u6291\u5236\u4f5c\u7528\uff0c\u900f\u5c04\u7535\u955c\u89c2\u5bdf\u836f\u7269\u4f5c\u7528\u540eHela \u7ec6\u80de\u5f62\u6001\u5b66\u53d8\u5316\uff0cDNA ladder \u5b9e\u9a8c\u89c2\u5bdfDNA \u7684\u53d8\u5316\u3002\u9ad8\u6548\u6db2\u76f8\u8272\u8c31-\u8d28\u8c31\u8054\u7528\u5206\u6790LQ-4 \u7684\u6210\u5206\u3002\u7ed3\u679c\uff1aMTT \u5b9e\u9a8c\u7ed3\u679c\u663e\u793a\u8fde\u7fd8\u63d0\u53d6\u7269LQ-4\u53ef\u4ee5\u6291\u5236Hela \u7ec6\u80de\u7684\u589e\u6b96\uff0c\u5e76\u6709\u65f6\u95f4\u5242\u91cf\u4f9d\u8d56\u6027\u3002LQ-4 \u4f5c\u7528\u4e8eHela \u7ec6\u80de12\u300124 \u548c48 h \u7684\u534a\u6570\u6291\u5236\u6d53\u5ea6\uff08IC50<\/sub>\uff09\u5206\u522b\u4e3a97.68\u300139.16\u300125.83 \u03bcg\/ml\u3002\u900f\u5c04\u7535\u955c\u663e\u793a\u7ec6\u80de\u53d1\u751f\u4e86\u51cb\u4ea1\u5f62\u6001\u53d8\u5316\uff0cDNA ladder \u5b9e\u9a8c\u663e\u793a\u4e86LQ-4 \u53ef\u5f15\u8d77\u7ec6\u80deDNA \u7684\u65ad\u88c2\u3002\u9ad8\u6548\u6db2\u8d28\u8054\u7528\u5206\u6790\u786e\u5b9aLQ-4 \u4e2d\u6297\u80bf\u7624\u6d3b\u6027\u6210\u5206\u4e94\u73af\u4e09\u841c\u7c7b\u6210\u5206\u7684\u5b58\u5728\u3002\u7ed3\u8bba\uff1a\u8fde\u7fd8\u63d0\u53d6\u7269LQ-4 \u4f53\u5916\u660e\u663e\u6291\u5236Hela \u7ec6\u80de\u7684\u589e\u6b96\u5e76\u53ef\u8bf1\u5bfc\u7ec6\u80de\u51cb\u4ea1\u3002<\/p>\n

\u5468\u6d77\u65ed\u91c7\u7528MTS \u6cd5\u68c0\u6d4b\u4e86\u677e\u8102\u7d20-4-O-\u03b2-D- \u5421\u5583\u8461\u8404\u7cd6\u82f7\u3001\u677e\u8102\u7d20\u5bf9\u5bab\u9888\u764cHeLa \u7ec6\u80de\u548cHUVEC\u7ec6\u80de\u751f\u957f\u6291\u5236\u4f5c\u7528\uff0c\u5b9e\u9a8c\u8868\u660e\uff0c\u677e\u8102\u7d20-4-O-\u03b2-D-\u5421\u5583\u8461\u8404\u7cd6\u82f7\u5bf9\u5bab\u9888\u764c\u7ec6\u80de\u6ca1\u6709\u4f5c\u7528\uff0c\u800c\u677e\u8102\u7d20\u53ef\u4ee5\u6291\u5236\u5bab\u9888\u764c\u7ec6\u80de\u7684\u589e\u6b96\u5e76\u4e14\u5b58\u5728\u6d53\u5ea6-\u6548\u5e94\u5173\u7cfb\uff0c\u5e76\u4e14\u5176\u5bf9\u6b63\u5e38\u4e0a\u76ae\u7ec6\u80de\u5728\u6d53\u5ea6\u5c0f\u4e8e200 \u03bcg\/ml\u65f6\u65e0\u6bd2\u6548\u679c\u3002<\/p>\n

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Bingyuan Du \u7b49\u5728\u7814\u7a76\u8fde\u7fd8\u8102\u7d20\u5bf9\u4f53\u5916\u4e1d\u88c2\u539f\u6d3b\u5316\u523a\u6fc0\u6dcb\u5df4\u7ec6\u80de\u7684\u6297\u708e\u4f5c\u7528\u65f6\u53d1\u73b0\uff0c\u8fde\u7fd8\u8102\u7d20\u53ef\u4ee5\u6291\u5236Con A \u6fc0\u6d3b\u7684\u6dcb\u5df4\u7ec6\u80de\u589e\u6b96\uff0c\u901a\u8fc7\u6291\u5236\u7ec6\u80de\u5468\u671f\u86cb\u767dD1 \u548c\u7ec6\u80de\u5468\u671f\u86cb\u767dE \u8bf1\u5bfcG0\/G1\u671f\u963b\u6ede\u3002\u540c\u65f6\uff0c\u8fde\u7fd8\u8102\u7d20\u901a\u8fc7\u963b\u65ad\u7ec6\u80de\u5185Ca2+<\/sup>\u8d85\u8f7d\u548c\u6291\u5236\u6765\u62ee\u6297Con A \u8bf1\u5bfc\u7684T \u7ec6\u80de\u6d3b\u5316\u3002<\/p>\n

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Do-Gyeong Lee \u7b49\u7814\u7a76\u5728\u8102\u591a\u7cd6\u523a\u6fc0\u7684\u5de8\u566c\u7ec6\u80deRAW264.7 \u4e2d\u8bc4\u4ef7\u55dc\u9178\u6027\u7c92\u7ec6\u80de\u5bf9NO \u4ea7\u751f\u7684\u6291\u5236\u6d3b\u6027\uff0c\u53d1\u73b0\u8fde\u7fd8\u8102\u7d20\u5bf9NO \u4ea7\u751f\u7684\u6291\u5236\u6d3b\u6027\u5f3a\uff0cIC50<\/sub> \u503c\u4e3a25.5\u3002<\/p>\n

2008 \u5e74Kang HS\u7b49\u7814\u7a76\u53d1\u73b0\uff0c\u8fde\u7fd8\u7684\u7532\u9187\u63d0\u53d6\u7269\u6709\u660e\u663e\u7684\u6297\u708e\u6d3b\u6027\uff0c\u4e14\u53d1\u73b0\u725b\u84a1\u82f7\u5143\u662f\u8fde\u7fd8\u679c\u5b9e\u7684\u4e00\u4e2a\u4e3b\u8981\u7684\u6d3b\u6027\u4e2d\u4ecb\u7269\u8d28\uff0c\u5b83\u6709\u663e\u8457\u7684\u6297\u708e\u548c\u6b62\u75db\u4f5c\u7528\uff0c\u5728\u6025\u6027\u708e\u75c7\u52a8\u7269\u5b9e\u9a8c\u6a21\u578b\u4e2d\uff0c\u5b83\u53ef\u4ee5\u6291\u5236\u6bdb\u7ec6\u7ba1\u901a\u900f\u6027\u7684\u589e\u52a0\u3001\u8865\u5145\u53d1\u708e\u7ec4\u7ec7\u4e2d\u7684\u767d\u8840\u7403\u3002<\/p>\n

\u5927\u9f20\u5df4\u8c46\u6cb9\u6027\u8089\u82bd\u56ca\u5b9e\u9a8c\u8bc1\u660e\uff0c\u8fde\u7fd8\u9187\u63d0\u53d6\u7269\u7684\u6c34\u6eb6\u6db2\u8179\u8154\u6ce8\u5c04\u6709\u975e\u5e38\u660e\u663e\u7684\u6297\u6e17\u51fa\u4f5c\u7528\u53ca\u964d\u4f4e\u708e\u6027\u90e8\u4f4d\u8840\u7ba1\u58c1\u8106\u6027\u4f5c\u7528\uff0c\u800c\u5bf9\u708e\u6027\u5c4f\u969c\u7684\u5f62\u6210\u65e0\u6291\u5236\u4f5c\u7528\u3002\u752832P\u6807\u8bb0\u7ea2\u7ec6\u80de\u5b9e\u9a8c\u4e5f\u89c2\u5bdf\u5230\u5176\u6e17\u5165\u5df2\u6ce8\u5c04\u8fde\u7fd8\u63d0\u53d6\u7269\u6c34\u6eb6\u6db2\u7684\u5927\u9f20\u5df4\u8c46\u6cb9\u6027\u8089\u82bd\u56ca\u5185\u7684\u6570\u91cf\u660e\u663e\u51cf\u5c11\uff0c\u8868\u660e\u8fde\u7fd8\u5c1a\u80fd\u4fc3\u8fdb\u708e\u6027\u5c4f\u969c\u7684\u5f62\u6210\u3002<\/p>\n

\u738b\u8000\u7b49\u4ee5\u8102\u591a\u7cd6(LPS)\u523a\u6fc0RAW264\uff0e7\u7ec6\u80de\uff0c\u7528\u8fde\u7fd8\u63d0\u53d6\u7269\u8fdb\u884c\u5e72\u9884\uff0c\u6d4b\u5b9aNO\u548cTNF-\u03b1\u542b\u91cf\uff0cWestern blot\u6cd5\u68c0\u6d4biNOS\u86cb\u767d\u8868\u8fbe\u6c34\u5e73\u3002\u7ed3\u679c\u663e\u793a\u8fde\u7fd8\u572812\uff0e5\uff5e100 g\u00b7mL \u6d53\u5ea6\u8303\u56f4\u5185\u53ef\u660e\u663e\u6291\u5236RAW264\uff0e7\u7ec6\u80de\u91ca\u653eTNF-\u03b1\u53caNO\uff0c\u5e76\u53ef\u663e\u8457\u6291\u5236iNOS\u86cb\u767d\u8868\u8fbe\uff1b\u8bf4\u660e\u8fde\u7fd8\u901a\u8fc7\u4e0b\u8c03iNOS\u86cb\u767d\u8868\u8fbe\uff0c\u6291\u5236\u708e\u75c7\u4ecb\u8d28TNF-\u03b1\u53caNO\u800c\u53d1\u6325\u6297\u708e\u4f5c\u7528\u3002<\/p>\n

\u5434\u4fca\u7b49\u7814\u7a76\u8bc1\u660e\uff0c\u91c7\u7528\u4e8c\u7532\u82ef\u81f4\u5c0f\u767d\u9f20\u8033\u5ed3\u80bf\u80c0\u975e\u7279\u5f02\u6027\u708e\u75c7\u6a21\u578b\uff0c\u94f6\u82b1\u8fde\u7fd8\u6df7\u714e\u6297\u708e\u4f5c\u7528\u6700\u5f3a\uff0c\u5408\u5e76\u6db2\u4f5c\u7528\u8f83\u5f3a\uff0c\u91d1\u94f6\u82b1\u63d0\u53d6\u6db2\u6297\u708e\u4f5c\u7528\u4e0d\u660e\u663e\u3002<\/p>\n

\u738b\u5a77\u5a77\u7b49\u9009\u62e9\u7814\u7a76\u5bf9\u8c61\u4e3a\u8fde\u7fd8\u916f\u82f7A\u542b\u91cf\u4e0d\u540c\u6c34\u5e73\u76844\u6279\u8fde\u7fd8\u548c1\u6279\u8fde\u7fd8\u53f6\uff0c\u5efa\u7acb\u4e8c\u7532\u82ef\u81f4\u5c0f\u9f20\u8033\u80bf\u80c0\u548c\u68c9\u7403\u6240\u81f4\u5927\u9f20\u8089\u82bd\u80bf\u589e\u751f\u5b9e\u9a8c\u4e24\u4e2a\u6a21\u578b\uff0c\u5206\u522b\u8bc4\u4ef7\u8fde\u7fd8\u7684\u6297\u708e\u4f5c\u7528\uff0c\u7ed3\u679c\u8868\u660e\uff0c\u8fde\u7fd8\u53f6\u5bf9\u8033\u80bf\u80c0\u6291\u5236\u6548\u679c\u4e00\u822c\uff0c\u4f46\u5bf9\u68c9\u7403\u8089\u82bd\u80bf\u7684\u6291\u5236\u6548\u679c\u6700\u597d\uff0c\u8bf4\u660e\u8fde\u7fd8\u53f6\u5bf9\u6162\u6027\u708e\u75c7\u6cbb\u7597\u6548\u679c\u5f88\u597d\uff0c\u5e76\u4e14\u6297\u708e\u6d3b\u6027\u4e0e\u8fde\u7fd8\u916f\u82f7A\u3001\u8fde\u7fd8\u82f7\u542b\u91cf\u65e0\u5173\u3002<\/p>\n

2.5 \u6297\u8fc7\u654f\u6d3b\u6027<\/p>\n

2010 \u5e74Hao Y\u7b49\u53d1\u73b0\uff0c\u8fde\u7fd8\u63d0\u53d6\u7269\u80fd\u51cf\u8f7b\u7531\u7cbe\u5236\u5927\u8c46\u03b2-\u4f34\u5927\u8c46\u7403\u86cb\u767d\u5f15\u8d77\u7684\u732a\u5e7c\u4ed4\u8fc7\u654f\u53cd\u5e94\uff0c\u80fd\u663e\u8457\u51cf\u5c11\u8fc7\u654f\u6297\u4f53\u3001\u80a5\u5927\u7ec6\u80de\u8131\u7c92\u548c\u7ec4\u80fa\u7684\u91ca\u653e\uff0c\u589e\u52a0\u80a0\u5185\u7684\u5fae\u751f\u7269\u83cc\u4e1b\uff0c\u8fd8\u80fd\u663e\u8457\u6291\u5236T\u6dcb\u5df4\u7ec6\u80de\u589e\u6b96\u548c\u767d\u4ecb\u7d20IL-4 \u7684\u5408\u6210\u3002<\/p>\n

\u91cd\u7528\u8fde\u7fd8\u8fd8\u53ef\u4ee5\u7528\u4e8e\u6cbb\u7597\u8fc7\u654f\u6027\u7d2b\u765c\u3002<\/p>\n

2.6 \u6297\u75c5\u6bd2\u4f5c\u7528 <\/p>\n

\u8fde\u7fd8\u7528\u4e8e\u6cbb\u7597\u5bf9\u7532\u578b\u6d41\u611f\u75c5\u6bd2\u3001\u4eba\u5de8\u7ec6\u80de\u75c5\u6bd2\u3001\u4e59\u578b\u8111\u708e\u75c5\u6bd2\u3001\u547c\u5438\u9053\u5408\u80de\u75c5\u6bd2\u3001\u5355\u7eaf\u75b1\u75b9\u75c5\u6bd2\u7b49\u75c5\u6bd2\u6709\u8f83\u597d\u7684\u6548\u679c\u3002<\/p>\n

\u9648\u6768\u7b49\u5148\u901a\u8fc7\u6c34\u63d0\u3001\u9187\u6c89\u3001\u5927\u5b54\u5438\u9644\u6811\u8102\u67f1\u5c42\u6790\u6cd5\u5206\u79bb\u5230\u6297\u75c5\u6bd2\u6709\u6548\u90e8\u4f4dLC\uff0d4\uff0c\u7136\u540e\u91c7\u7528\u7ec6\u80de\u75c5\u53d8(CPE)\u89c2\u5bdf\u6cd5\u548c \u4e2d\u6027\u7ea2\u5b9e\u9a8c\u6cd5\u6d4b\u5b9aLC\uff0d4\u5bf9\u547c\u5438\u9053\u5408\u80de\u75c5\u6bd2(\uff32SV)\u7684\u6291\u5236\u4f5c \u7528\uff0c\u5b9e\u9a8c\u7ed3\u679c\u8868\u660e\uff0cLC\uff0d4\u5bf9\uff32SV\u75c5\u6bd2\u6709\u660e\u663e\u7684\u9884\u9632\u548c\u6cbb\u7597\u6291 \u5236\u4f5c\u7528\uff0c\u5e76\u4e14\u65e0\u8bba\u662f\u5728\u75c5\u6bd2\u590d\u5236\u7684\u65e9\u671f\u8fd8\u662f\u4e2d\u665a\u671f\uff0cLC\uff0d4\u5bf9 \u4e8e\u8fdb\u5165\u7ec6\u80de\u7684\u75c5\u6bd2\u90fd\u6709\u4e00\u5b9a\u7684\u6291\u5236\u4f5c\u7528\u3002<\/p>\n

\u8fde\u7fd8\u5c5e\u690d\u7269\u63d0\u53d6\u7269\u6709\u6297\u67ef\u8428\u5947B5\u75c5\u6bd2\u53ca\u57c3\u67ef\u75c5\u6bd2\u7684\u4f5c\u7528 \u3002\u67ef\u8428\u5947B\u7ec4\u75c5\u6bd2\u662f\u5f15\u8d77\u5fc3\u808c\u708e\u7684\u75c5\u56e0\u4e4b\u4e00\uff1b\u8fde\u7fd8\u5728\u5148\u52a0\u836f\u540e\u52a0\u75c5\u6bd2\u7ec4\u3001\u611f\u67d3\u75c5\u6bd2\u540c\u65f6\u52a0\u836f\u7ec4\u3001\u611f\u67d3\u75c5\u6bd2\u540e\u52a0\u836f\u7ec4\u4e2d\uff0c\u5747\u6709\u4e00\u5b9a\u7684\u6297\u75c5\u6bd2\u4f5c\u7528\u3002\u9e21\u80da\u4f53\u5916\u8bd5\u9a8c\u8bc1\u660e\u8fde\u7fd8\u5bf9\u4e9a\u6d32\u7532\u578b\u6d41\u611f\u75c5\u6bd2\u3001\u9f3b\u75c5\u6bd2\u7b49\u4e5f\u6709\u6291\u5236\u4f5c\u7528\u3002<\/p>\n

\u9a6c\u632f\u4e9a\u5b9e\u9a8c\u91c7\u7528\u9e21\u80da\u5185\u3001\u9e21\u80da\u5916\u5b9e\u9a8c\u8bc1\u5b9e\u8fde\u7fd8\u79cd\u5b50\u6325\u53d1\u6cb9\u5bf9\u4e9a\u6d32\u7532\u578b\u6d41\u611f\u75c5\u6bd2\u548c\u2160\u578b\u526f\u6d41\u611f\u75c5\u6bd2\u5177\u6709\u663e\u8457\u7684\u6291\u5236\u4f5c\u7528\u3002\u8096\u4f1a\u654f\u7b49\uff3b44\uff3d \u4ee5HepG2. 2. 15 \u7ec6\u80de\u4e3a\u4e59\u578b\u809d\u708e\u75c5\u6bd2\u8f7d\u4f53\uff0c\u5728\u8fde\u7fd8\u6325\u53d1\u6cb9( 258. 69 \u03bcg \/mL) \u3001\u03b1-\u848e\u70ef( 258. 69 \u03bcg \/mL) \u3001\u03b2-\u848e\u70ef( 192. 45 \u03bcg \/mL) \u4e3a\u6700\u5927\u65e0\u6bd2\u5242\u91cf\u65f6\uff0c\u5747\u65e0\u6291\u5236\u4e59\u578b\u809d\u708e\u75c5\u6bd2\u590d\u5236DNA \u548c\u4ea7\u751fHBsAg\u3001HBeAg \u7684\u80fd\u529b\uff1b\u5728\u5242\u91cf\u5747\u4e3a192. 5 \u03bcg \/mL \u65f6\uff0c\u8fde\u7fd8\u6325\u53d1\u6cb9\u3001\u03b1-\u848e\u70ef\u3001\u03b2-\u848e\u70ef\u5747\u65e0\u6297\u7532\u3001\u4e59\u578b\u6d41\u611f\u75c5\u6bd2\u6d3b\u6027\u3002\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\u8fde\u7fd8\u6325\u53d1\u6cb9\u4e2d\u5176\u4ed6\u6210\u5206\u53d1\u6325\u6297\u75c5\u6bd2\u4f5c\u7528\u800c\u4e0d\u662f\u6240\u6d4b\u8bd5\u7684\u5316\u5408\u7269\uff0c\u6216\u8fde\u7fd8\u6325\u53d1\u6cb9\u6fc0\u6d3b\u5c0f\u9f20\u4f53\u5185\u5176\u4ed6\u9014\u5f84\u6291\u5236\u6d41\u611f\u75c5\u6bd2\u800c\u4e0d\u662f\u76f4\u63a5\u7684\u4f5c\u7528\u3002<\/p>\n

\u6bb5\u6797\u5efa\u7b49\u7528\u7532\u578b\u6d41\u611f\u75c5\u6bd2\u6838\u86cb\u767d(NP)\u57fa\u56e0\u8f6c\u67d3Hela\u7ec6\u80de\uff0c\u7528\u7532\u578b\u6d41\u611f\u75c5\u6bd2\u80f6\u4f53\u91d1\u6cd5\u68c0\u6d4b\u8fde\u7fd8\u82f7\u5bf9\u8f6c\u67d3\u540e\u7ec6\u80de\u5185\u548c\u4e0a\u6e05\u6838\u86cb\u767d\u8868\u8fbe\u60c5\u51b5\uff0c\u7528\u5b9e\u65f6\u5b9a\u91cf\u53cd\u8f6c\u5f55\u805a\u5408\u9176\u94fe\u53cd\u5e94(RT\u2014PCR)\u68c0\u6d4bHela\u7ec6\u80de\u5185NP\u57fa\u56e0\u7684\u62f7\u8d1d\u6570\u3002\u91cd\u590d4\u6b21\u8f6c\u67d3\u540e48 h\u8fde\u7fd8\u82f7\u7ec4NP\u57fa\u56e0\u8868\u8fbe\u91cf\u4e3a(2\uff0e1\u00b10\uff0e3)x 105<\/sup>\u62f7\u8d1d\u6570\u00b7\u03bcL-1<\/sup>\uff0cNP\u91cd\u7ec4\u8d28\u7c92\u7ec4NP\u57fa\u56e0\u8868\u8fbe\u91cf\u4e3a(61\uff0e5\u00b115\uff0e0)\u00d7105<\/sup>\u62f7\u8d1d\u6570\u00b7\u03bcL-1<\/sup>\uff0c\u8fde\u7fd8\u82f7\u7ec4NP\u57fa\u56e0\u8868\u8fbe\u91cf\u4f4e\u4e8eNP\u91cd\u7ec4\u8d28\u7c92\u7ec4\u3002\u8bf4\u660e\u8fde\u7fd8\u82f7\u53ef\u4ee5\u6291\u5236\u7532\u578b\u6d41\u611f\u75c5\u6bd2NP\u57fa\u56e0\u8f6c\u67d3\u540e\u8868\u8fbe\u3002<\/p>\n

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2.7 \u6297\u5185\u6bd2\u7d20\u4f5c\u7528<\/p>\n

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\u6297PAN X\u7b49\u5229\u7528\u5916\u5468\u5de8\u566c\u7ec6\u80de\uff32AW264\uff0e7\u7ec6\u80de\u682a\u6765\u7814\u7a76\u8fde\u7fd8 \u5bf9\u5185\u6bd2\u7d20(LPS)\u8bf1\u5bfc\u7684\u708e\u75c7\u53cd\u5e94\u7684\u5f71\u54cd\u53ca\u5176\u53ef\u80fd\u7684\u5206\u5b50\u673a\u5236\u3002\u6700\u7ec8\u53d1\u73b0\u8fde\u7fd8\u53ef\u80fd\u901a\u8fc7\u6291\u5236\uff32AW264\uff0e7\u7ec6\u80de\u4e2dJAK\uff0dSTATs \u548cp38MAPKs\u4fe1\u53f7\u901a\u8def\uff0c\u4ece\u800c\u51cf\u5c11\u5185\u6bd2\u7d20\u8bf1\u5bfc\u7684\u4fc3\u708e\u56e0\u5b50\u7684\u91ca\u653e\u3002<\/p>\n

2.8 \u6291\u5236\u5f39\u6027\u86cb\u767d\u9176\u6d3b\u529b\u7684\u4f5c\u7528<\/p>\n

\u5f39\u6027\u86cb\u767d\u9176\u662f\u4e00\u79cd\u86cb\u767d\u6c34\u89e3\u9176\uff0c\u80fd\u5206\u89e3\u80ba\u6ce1\u58c1\u7684\u5f39\u529b\u7ea4\u7ef4\uff0c\u4eba\u4f53\u5185\u7684\u5f39\u6027\u86cb\u767d\u9176\u4e3b\u8981\u6765\u6e90\u4e8e\u4e2d\u6027\u7c92\u7ec6\u80de\u53ca\u80ba\u6ce1\u5de8\u566c\u7ec6\u80de\u3002\u6162\u6027\u963b\u585e\u6027\u80ba\u75be\u75c5(COPD)\u60a3\u8005\u4f53\u5185\u5f39\u6027\u86cb\u767d\u9176\u6291\u5236\u5242\u5148\u5929\u6027\u7f3a\u4e4f\u6216\u6d3b\u529b\u4e0b\u964d\uff0c\u56e0\u800c\u68c0\u6d4b\u6162\u6027\u963b\u585e\u6027\u80ba\u75be\u75c5\u60a3\u8005\u4f53\u5185\u5f39\u6027\u86cb\u767d\u9176\u6d3b\u529b\u4ee5\u5bfb\u627e\u5f39\u6027\u86cb\u767d\u9176\u6291\u5236\u5242\u836f\u7269\u5df2\u5f15\u8d77\u4e34\u5e8a\u53ca\u5b9e\u9a8c\u5de5\u4f5c\u8005\u7684\u5173\u6ce8\u3002\u6709\u4eba\u4ee5\u743c\u8102\u6269\u6563\u6d4b\u5b9a\u6cd5\u6bd4\u8f83\u4e86\u8fde\u7fd8\u3001\u4e39\u53c2\u3001\u5f53\u5f52\u3001\u5ddd\u7a79\u55ea\u7b49\u6291\u5236\u5f39\u529b\u86cb\u767d\u9176\u6d3b\u529b\u5f3a\u5f31\uff0c\u7ed3\u679c\u8868\u660e\u8fd94\u79cd\u4e2d\u836f\u5bf9\u5f39\u6027\u86cb\u767d\u9176\u6d3b\u529b\u5747\u6709\u663e\u8457\u6291\u5236\u4f5c\u7528\uff0c\u5176\u4e2d\u4ee5\u8fde\u7fd8\u4e3a\u6700\u4f18\uff0c\u5f53\u8fde\u7fd8\u4e3a7\uff0e5
\nnag(2\uff0e5 g\uff0c\uff0fm1)\u65f6\u5373\u80fd\u5b8c\u5168\u6291\u5236\u8be5\u9176\u7684\u6d3b\u529b\uff0c\u800c\u7b49\u91cf\u7684\u4e39\u53c2\u3001\u5f53\u5f52\u3001\u5ddd\u7a79\u55ea\u867d\u5bf9\u5176\u6709\u663e\u8457\u6291\u5236\u4f5c\u7528\uff0c\u4f46\u5728\u743c\u8102\u677f\u4e0a\u4ecd\u51fa\u73b0\u4e86\u8be5\u9176\u7684\u6eb6\u89e3\u73af\u3002\u6240\u4ee5\u8fdb\u4e00\u6b65\u7814\u7a76\u8fde\u7fd8\u7684\u8fd9\u79cd\u4f5c\u7528\u5e76\u5206\u6790\u5176\u6709\u6548\u6210\u5206\uff0c\u53ef\u671b\u53d1\u6398\u51fa\u6291\u5236\u5f39\u6027\u86cb\u767d\u9176\u7684\u65b0\u836f\u3002<\/p>\n

2.9\u9547\u75db\u4f5c\u7528<\/p>\n

\u4ee5\u8fde\u7fd8\u4e3a\u4e3b\u836f\u7684\u7259\u75db\u7075\u5236\u5242(\u8fde\u7fd8\u6800\u5b50\u7b49)\u80fd\u63d0\u9ad8\u7535\u523a\u6fc0\u5bb6\u5154\u9f7f\u9ad3\u53ca\u5c0f\u9f20\u75db\u9619\u503c\uff0c\u51cf\u8f7b\u5c0f\u9f20\u56e0\u5df4\u8c46\u6cb9\u5f15\u8d77\u7684\u8033\u80bf\u80c0\uff0c\u6291\u5236\u708e\u6027\u8089\u82bd\u80bf\u3002\u52a8\u7269\u7684\u9547\u75db\u6297\u708e\u6291\u83cc\u5b9e\u9a8c\u8868\u660e\uff0c\u7259\u75db\u7075\u5bf9\u7269\u7406\u3001\u5316\u5b66\u53ca\u7535\u523a\u6fc0\u5f15\u8d77\u7684\u75bc\u75db\u6a21\u578b\u5747\u6709\u660e\u663e\u9547\u75db\u4f5c\u7528\uff0c\u4ea6\u80fd\u6291\u5236\u5404\u79cd\u81f4\u708e\u56e0\u5b50\u5f15\u8d77\u7684\u708e\u6027\u80bf\u80c0\u201d\u3002<\/p>\n

2.10 \u89e3\u70ed\u4f5c\u7528<\/p>\n

\u8fde\u7fd8\u7684\u73b0\u4ee3\u836f\u7406\u7814\u7a76\u7684\u89e3\u70ed\u4f5c\u7528\u4e0e\u5176\u4f20\u7edf\u4e2d\u533b\u8ba4\u4e3a\u7684\u758f\u6563\u98ce\u70ed\u3001\u6e05\u70ed\u89e3\u6bd2\u7684\u529f\u6548\u76f8\u4e00\u81f4\u3002\u82cf\u7ea2\u660c\u7b49\u8ba4\u4e3a\uff0c\u8fde\u7fd8\u916f\u82f7A\uff08forsythiaside
\nA\uff0cFA\uff09\u80fd\u964d\u4f4e\u7531\u9175\u6bcd\u83cc\u611f\u67d3\u5f15\u53d1\u5c0f\u9f20\u4f53\u6e29\u5347\u9ad8\u3002\u515a\u73cf\u7b49\u7814\u7a76\u8868\u660e\uff0c\u8fde\u7fd8\u63d0\u53d6\u7269\u548c\u8fde\u7fd8\u6325\u53d1\u6cb9\u5747\u5177\u6709\u4e0b\u8c03\u9175\u6bcd\u81f4\u70ed\u5927\u9f20\u4e0b\u4e18\u8111\u4e2dcAMP \u7684\u4f5c\u7528\uff0c\u4ece\u800c\u8fbe\u5230\u89e3\u70ed\u6548\u679c\u3002\u6b64\u5916\u8fde\u7fd8\u63d0\u53d6\u7269\u8fd8\u5177\u6709\u4e0b\u8c03\u4e0b\u4e18\u8111\u4e2dPGE2 \u7684\u4f5c\u7528\u3002<\/p>\n

\u8fde\u7fd8\u714e\u52424 g(\u751f\u836f)\uff0fkg\u704c\u80c3\uff0c\u80fd\u4f7f\u9759\u6ce8\u67af\u8349\u6746\u83cc\u6d78\u6db2\u6240\u81f4\u7684\u5bb6\u5154\u53d1\u70ed\u4f5c\u7528\u663e\u8457\u4e0b\u964d\uff0c1 h\u540e\u6062\u590d\u6b63\u5e38\uff0c\u968f\u540e\u8fd8\u53ef\u964d\u81f4\u6b63\u5e38\u4f53\u6e29\u4ee5\u4e0b\u3002\u590d\u65b9\u8fde\u7fd8\u6ce8\u5c04\u6db2\u4e5f\u6709\u660e\u663e\u7684\u89e3\u70ed\u4f5c\u7528\uff0c\u80fd\u51cf\u5f31\u4f24\u5bd2\u83cc\u82d7\u6240\u81f4\u5bb6\u5154\u53d1\u70ed\uff0c\u4e5f\u80fd\u964d\u4f4e\u6b63\u5e38\u5bb6\u5154\u7684\u4f53\u6e29\u3002<\/p>\n

\u515a\u73cf\u7b49\u91c7\u7528\u5927\u9f20\u53d1\u70ed\u6a21\u578b\uff0c\u7ed9\u836f\u5927\u9f20\u4f53\u6e29\u548c\u4e0b\u4e18\u8111cAMP \u548cPGE2\u542b\u91cf\u53d8\u5316\u8868\u660e\uff0c\u8fde\u7fd8\u6325\u53d1\u6cb9\u53ef\u663e\u8457\u964d\u4f4e\u9175\u6bcd\u81f4\u70ed\u5927\u9f20\u7684\u4f53\u6e29( P \uff1c 0.
\n01 \u6216P \uff1c0. 05) \uff0c\u80fd\u663e\u8457\u964d\u4f4e\u4e0b\u4e18\u8111\u4e2dcAMP( P \uff1c 0. 05) \u542b\u91cf\uff0c\u800c\u5bf9PGE2 \u542b\u91cf\u65e0\u6539\u53d8\uff0c\u53ef\u9488\u5bf9\u6027\u7528\u4e8e\u4e34\u5e8a\u6cbb\u7597\u3002<\/p>\n

\u7f57\u6797\u7b49\u91c7\u7528\u8db3\u76ae\u4e0b\u6ce8\u5c04\u89d2\u53c9\u83dc\u80f6\u3001\u9e21\u86cb\u6e05\u5927\u9f20\u8db3\u80bf\u80c0\u6a21\u578b\u4ee5\u53ca\u5927\u9f20\u68c9\u7403\u8089\u82bd\u80bf\u6a21\u578b\u7814\u7a76\u53d1\u73b0\u8fde\u7fd8\u6325\u53d1\u6cb9\u53ef\u6291\u5236\u8102\u591a\u7cd6\u548c\u5e72\u9175\u6bcd\u6240\u81f4\u53d1\u70ed\uff0c\u964d\u4f4e\u89d2\u53c9\u83dc\u80f6\u548c\u9e21\u86cb\u6e05\u6240\u8bf1\u5bfc\u7684\u5927\u9f20\u8db3\u80bf\u80c0\uff0c\u4e14\u53ef\u663e\u8457\u6291\u5236\u8089\u82bd\u7ec4\u7ec7\u7684\u589e\u751f\u3002\u7531\u4e8e\u8fde\u7fd8\u6325\u53d1\u6cb9\u89e3\u70ed\u3001\u6297\u708e\u4f5c\u7528\u5df2\u5f97\u5230\u5e7f\u6cdb\u5e94\u7528\uff0c\u5176\u89e3\u70ed\u6297\u708e\u836f\u6548\u7684\u673a\u7406\u7814\u7a76\u4e3a\u5176\u8fdb\u4e00\u6b65\u5f00\u53d1\u63d0\u4f9b\u79d1\u5b66\u4f9d\u636e\u3002<\/p>\n

2.11\u4fdd\u809d\u5229\u80c6\u4f5c\u7528<\/p>\n

\u8fde\u7fd8\u63d0\u53d6\u7269\u5177\u6709\u5229\u80c6\u4fdd\u809d\u7684\u4f5c\u7528\u3002<\/p>\n

ZHAO P F\u7b49\u901a\u8fc7\u5b9e\u9a8c\u7814\u7a76\u53d1\u73b0\uff0c\u8fde\u7fd8\u63d0\u53d6\u7269(FSE)\u53ef\u4ee5\u51cf\u8f7bLPS\u8bf1\u5bfc\u7684\u809d\u7ec4\u7ec7 \u80de\u6d46\u6838\u56e0\u5b50\uff0d\u76f8\u5173\u56e0\u5b502(Nrf2)\u7684\u8017\u7aed\u548cNrf2\u6838\u8f6c\u4f4d\u7684\u6291\u5236\uff0c \u51cf\u8f7b\u8840\u6e05\u548c\u809d\u810f\uff32OS\u548c\u4e19\u4e8c\u919b\u7684\u4ea7\u751f\uff0c\u589e\u52a0Nrf2\u4ecb\u5bfc\u7684\u809d\u810f \u8840\u7ea2\u7d20\u52a0\u6c27\u9176\uff0d1\u7684\u8bf1\u5bfc\uff0c\u4ee5\u53ca\u589e\u52a0\u8840\u6e05\u548c\u809d\u810f\u4e2d\u8d85\u6c27\u5316\u7269\u6b67 \u5316\u9176\u548c\u8c37\u80f1\u7518\u80bd\u8fc7\u6c27\u5316\u7269\u9176\u7684\u6d3b\u6027\u3002\u7ed3\u679c\u8868\u660e\uff0c\u8fde\u7fd8\u63d0\u53d6\u7269\u53ef \u80fd\u901a\u8fc7\u8c03\u8282N\uff32F2\u4ecb\u5bfc\u7684\u6297\u6c27\u5316\u53cd\u5e94\uff0c\u51cf\u8f7b\u6c27\u5316\u5e94\u6fc0\uff0c\u6291\u5236 NF\uff0d\u03baB\u4ecb\u5bfc\u7684\u708e\u75c7\u53cd\u5e94\uff0c\u4ece\u800c\u51cf\u8f7b\u6c27\u5316\u5e94\u6fc0\u548c\u6291\u5236NF\uff0d N\uff32FB\u4ecb\u5bfc\u7684\u708e\u6027\u809d\u635f\u4f24\uff0c\u8d77\u5230\u4fdd\u62a4\u809d\u810f\u7684\u4f5c\u7528\u3002<\/p>\n

\u51af\u82b9\u7b49\u6d4b\u5b9a\u88abCCl4<\/sub>\u8bf1\u5bfc\u6025\u6027\u809d\u635f\u4f24\u5927\u9f20\u8840\u6e05\u4e2d\u4e19\u6c28\u9178\u6c28\u57fa\u8f6c\u79fb\u9176\uff08ALT\uff09\u3001\u5929\u51ac\u6c28\u9178\u6c28\u57fa\u8f6c\u79fb\u9176\uff08AST\uff09\u3001\u603b\u80c6\u7ea2\u7d20\uff08TBIL\uff09\u6c34\u5e73\uff0c\u53d1\u73b0\u8fde\u7fd8\u82f7\u5143\u53ef\u660e\u663e\u964d\u4f4e\u8840\u6e05\u4e2dALT\u3001AST \u548cTBIL \u6c34\u5e73\uff0c\u7531\u6b64\u8bf4\u660e\u8fde\u7fd8\u82f7\u5143\u5177\u6709\u4fdd\u809d\u4f5c\u7528\u3002\u8fde\u7fd8\u82f7\u5143\u4fdd\u809d\u7684\u4f5c\u7528\u673a\u5236\u4e0e\u5176\u589e\u52a0\u809d\u7ec4\u7ec7\u4e2d\u6297\u6c27\u5316\u9176\u7684\u6d3b\u6027\u3001\u964d\u4f4e\u8102\u8d28\u8fc7\u6c27\u5316\u6c34\u5e73\u3001\u964d\u4f4eTNF-\u03b1\u3001IL-8\u7b49\u4fc3\u708e\u56e0\u5b50\u6c34\u5e73\u6709\u5173\u3002<\/p>\n

\u8fde\u7fd8\u6c34\u716e\u6db2\u53ef\u660e\u663e\u51cf\u8f7b\u56db\u6c2f\u5316\u78b3\u6240\u81f4\u7684\u809d\u810f\u53d8\u6027\u548c\u574f\u6b7b\uff0c\u5e76\u4f7f\u809d\u7ec6\u80de\u5185\u84c4\u79ef\u7684\u809d\u7cd6\u539f\u3001\u6838\u7cd6\u6838\u9178\u5927\u90e8\u5206\u6062\u590d\u548c\u63a5\u8fd1\u6b63\u5e38\uff0c\u53ef\u4f7f\u8f6c\u6c28\u9176\u4e0b\u964d\u3002\u9f50\u58a9\u679c\u9178\u548c\u718a\u679c\u9178\u5bf9\u6025\u6027\u809d\u708e\u6709\u8f83\u597d\u7597\u6548\u3002<\/p>\n

\u6768\u7f8e\u5170\u7b49\u91c7\u7528\u80c6\u7ba1\u5f15\u6d41\u6cd5\uff0c\u89c2\u5bdf\u8fde\u7fd8\u916f\u82f7\u5bf9\u6b63\u5e38\u9ebb\u9189\u5927\u9f20\u80c6\u6c41\u6d41\u91cf\u7684\u5f71\u54cd\uff0c\u5e76\u5206\u522b\u6d4b\u5b9a\u80c6\u6c41\u4e2d\u4e3b\u8981\u6210\u5206\uff0c\u7ed3\u679c\u8868\u660e\uff0c\u8fde\u7fd8\u916f\u82f7\u53ef\u663e\u8457\u589e\u52a0\u9ebb\u9189\u5927\u9f20\u80c6\u6c41\u7684\u6d41\u91cf\uff0c\u5448\u73b0\u6bd4\u8f83\u660e\u663e\u7684\u91cf\u6548\u5173\u7cfb\uff0c\u4f46\u5bf9\u80c6\u6c41\u6210\u5206\u5f71\u54cd\u4e0d\u660e\u663e\uff0c\u8bf4\u660e\u8fde\u7fd8\u916f\u82f7\u6709\u660e\u663e\u7684\u5229\u80c6\u4f5c\u7528\u3002<\/p>\n

2.12 \u9547\u5410\u4f5c\u7528<\/p>\n

\u8042\u514b\u7b49\u7528\u963f\u6734\u5417\u5561\u3001\u786b\u9178\u94dc\u3001\u987a\u94c23\u79cd\u7ecf\u5178\u50ac\u5410\u5242\u5efa\u7acb\u4e86\u5927\u9f20\u5f02\u98df\u7656\u6a21\u578b\uff0c\u5b9e\u9a8c\u7ec4\u548c\u5bf9\u7167\u7ec4\u7814\u7a76\u7ed3\u679c\u663e\u793a\u8fde\u7fd8\u5bf9\u4e0d\u540c\u50ac\u5410\u5242\u5bfc\u81f4\u7684\u5927\u9f20\u5f02\u98df\u7656\u5177\u6709\u663e\u8457\u6291\u5236\u4f5c\u7528\uff0c\u8bf4\u660e\u8fde\u7fd8\u63d0\u53d6\u7269\u5177\u6709\u826f\u597d\u7684\u9547\u5410\u6b62\u5455\u4f5c\u7528\u3002<\/p>\n

\u8fde\u7fd8\u714e\u5242\u704c\u80c3\uff0c\u80fd\u5bf9\u6297\u6d0b\u5730\u9ec4\u548c\u963f\u6734\u5417\u5561\u5f15\u8d77\u7684\u52a8\u7269\u5455\u5410\u3002\u5176\u6b62\u5410\u4f5c\u7528\u53ef\u80fd\u4e0e\u6291\u5236\u5ef6\u9ad3\u7684\u50ac\u5410\u5316\u5b66\u611f\u53d7\u533a\u6709\u5173\u3002<\/p>\n

2.13 \u5bf9\u5fc3\u8111\u8840\u7ba1\u7684\u5f71\u54cd<\/p>\n

\u4f0f\u79cb\u7b49\u91c7\u7528\u4e3b\u52a8\u8109\u5f13\u7f29\u7a84\u5efa\u7acb\u5c0f\u9f20\u538b\u529b\u8d1f\u8377\u6027\u5fc3\u529b\u8870\u7aed(HF)\uff0c\u63a2\u7a76\u8fde\u7fd8\u916f\u82f7A(FTA)\u5bf9\u5c0f\u9f20\u538b\u529b\u8d1f\u8377\u6027(HF)\u7684\u4fdd\u62a4\u4f5c\u7528\u53ca\u5176\u6f5c\u5728\u7684\u673a\u5236\u3002\u7ed3\u679c\u8868\u660eFTA\u5904\u7406\u6fc0\u6d3b\u6c27\u5316\u5e94\u6fc0Nrf2\/HO\uff0d1\u4fe1\u53f7\u901a\u8def\uff0c\u964d\u4f4eNF\uff0d\u03baB\u86cb\u767d\u8868\u8fbe\uff0c\u5bf9HF\u5c0f\u9f20\u5fc3\u810f\u635f\u4f24\u53d1\u6325\u4fdd\u62a4\u4f5c\u7528\u3002<\/p>\n

Wang \u7b49\u7814\u7a76\u4e86\u8fde\u7fd8\u916f\u82f7\uff08forsythiaside\uff0cFTS\uff09\u5bf9\u5feb\u901f\u8001\u5316\u6a21\u578b\uff08SAMP8\uff09\u5c0f\u9f20\u7684\u5f71\u54cd\uff0c\u7ed3\u679c\u8868\u660e\uff0cFTS \u5bf9SAMP8 \u5c0f\u9f20\u8ba4\u77e5\u969c\u788d\u5177\u6709\u4e00\u5b9a\u7684\u6539\u5584\u4f5c\u7528\uff0c\u5e76\u5bf9\u5176\u795e\u7ecf\u5177\u6709\u4e00\u5b9a\u7684\u4fdd\u62a4\u4f5c\u7528\uff0c\u53ef\u7528\u4e8e\u5065\u5fd8\u75c7\u7684\u6cbb\u7597\u3002<\/p>\n

Kim \u7b49\u7814\u7a76\u8868\u660e\uff0c\u8fde\u7fd8\u7684\u4e3b\u8981\u6210\u5206\u8fde\u7fd8\u916f\u82f7\uff08FTS\uff09\u5bf9\u77ed\u6682\u6027\u8111\u7f3a\u8840\u6c99\u9f20\u7684\u795e\u7ecf\u6709\u4fdd\u62a4\u4f5c\u7528\uff0c\u5176\u673a\u5236\u53ef\u80fd\u4e0eFTS \u8bf1\u5bfc\u6d3b\u5316\u7684\u795e\u7ecf\u80f6\u8d28\u7ec6\u80de\u7684\u51cf\u5c11\u53caIL-1\u03b2\u3001TNF-\u03b1 \u6c34\u5e73\u7684\u63d0\u9ad8\u6709\u5173\u3002<\/p>\n

Iizuka \u7b49\u901a\u8fc7\u5927\u9f20\u4e3b\u52a8\u8109\u73af\u79bb\u4f53\u5b9e\u9a8c\u7814\u7a76\u4e86\u8fde\u7fd8\u916f\u82f7\uff08FTS\uff09\u7684\u8840\u7ba1\u8212\u5f20\u4f5c\u7528\uff0c\u7ed3\u679c\u8868\u660e\uff0cFTS \u53ea\u80fd\u6291\u5236\u7531NE\u5f15\u8d77\u7684\u8840\u7ba1\u6536\u7f29\uff0c\u5e76\u63a8\u6d4b\u5176\u4f5c\u7528\u673a\u5236\u53ef\u80fd\u662f\u51cf\u5c11NE \u5f15\u8d77\u7684Ca2+<\/sup>\u5185\u6d41\u6709\u5173\u3002<\/p>\n

\u9f50\u58a9\u679c\u9178\u6709\u8f7b\u5ea6\u7684\u5f3a\u5fc3\u4f5c\u7528\u3002\u6025\u6027\u52a8\u7269\u5b9e\u9a8c\u8bc1\u660e\uff0c\u8fde\u7fd8\u6ce8\u5c04\u6db2\u6709\u964d\u538b\u4f5c\u7528\u3002\u4e00\u822c\u8840\u538b\u4e0b\u964d\u53ef\u8fbe\u5230\u539f\u6c34\u5e73\u768440\uff05 \uff5e60\uff05 \uff0c\u5176\u964d\u538b\u7279\u70b9\u4e3a\u8fc5\u901f\u3001\u663e\u8457\u3001\u6301\u7eed\u65f6\u95f4\u8f83\u77ed\uff0c\u591a\u6b21\u6ce8\u5c04\u65e0\u5feb\u901f\u8010\u53d7\u6027\u3002\u5154\u8033\u704c\u6d41\u5b9e\u9a8c\u8bc1\u660e\uff0c\u5bf9\u5916\u5468\u8840\u7ba1\u6709\u76f4\u63a5\u6269\u5f20\u4f5c\u7528\u3002<\/p>\n

2.14 \u5229\u5c3f\u4f5c\u7528<\/p>\n

\u9f50\u58a9\u679c\u9178\u6709\u8f7b\u5fae\u7684\u5229\u5c3f\u4f5c\u7528\u3002100\uff05\u8fde\u7fd8\u6ce8\u5c04\u6db20\uff0e25g\uff0fkg\u9759\u6ce8\uff0c\u5bf9\u9ebb\u9189\u72ac\u6709\u663e\u8457\u7684\u5229\u5c3f\u4f5c\u7528\uff0c\u5728\u7ed9\u836f0\uff0e5\u20141 h\u540e\u6700\u660e\u663e\u3002<\/p>\n

2.15 \u6297\u8870\u8001\u4f5c\u7528<\/p>\n

\u989c\u793c\u6709\u7b49\u59dc\u6d9b\u7b49\u901a\u8fc7\u5efa\u7acb\u8fde\u7fd8\u6307\u7eb9\u56fe\u8c31\u4e0e\u6297\u83cc\u6d3b\u6027\u4e4b\u95f4\u7684\u8c31\u6548\u5173\u7cfb\uff0c\u53d1\u73b0\u5728\u8fde\u7fd8\u6307\u7eb9\u56fe\u8c31\u4e2d\uff0c\u8fde\u7fd8\u916f\u82f7A\u3001\u5496\u5561\u9178\u3001\u8fde\u7fd8\u82f7\u548c\u8fde\u7fd8\u8102\u7d20\u5f71\u54cd\u8fde\u7fd8\u5bf9\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u6d3b\u6027\u7684\u6291\u5236\u4f5c\u7528\u3002\u8fde\u7fd8\u6291\u83cc\u7684\u6d3b\u6027\u6210\u5206\u53ef\u80fd\u4e3a\u8fde\u7fd8\u916f\u82f7\u3001\u5496\u5561\u9178\u3001\u8fde\u7fd8\u82f7\u548c\u8fde\u7fd8\u8102\u7d20\u3002\u91c7\u7528\u5f80\u5c0f\u9f20\u9888\u90e8\u6ce8\u5c04D-\u534a\u4e73\u7cd6\u6765\u5efa\u7acb\u8870\u8001\u5c0f\u9f20\u6a21\u578b\u7684\u65b9\u6cd5\uff0c\u6765\u6d4b\u5b9a\u5c0f\u9f20\u8111\u7ec4\u7ec7MDA\u3001B\u578b\u5355\u80fa\u6c27\u5316\u9176\uff08MAO-B\uff09\u3001SOD\u3001T-AOC\u6c34\u5e73\uff0c\u540c\u65f6\u6765\u6d4b\u5b9a\u5c0f\u9f20\u589e\u8d28\u91cf\u7387\u4e0e\u813e\u810f\u3001\u5fc3\u810f\u3001\u80be\u810f\u3001\u809d\u810f\u6307\u6570\uff0c\u6d4b\u5b9a\u5c0f\u9f20\u8840\u6e05\u3001\u809d\u7ec4\u7ec7\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u3001\u8c37\u80f1\u7518\u80bd\u8fc7\u6c27\u5316\u7269\u9176\uff08GSH-Px\uff09\u3001\u4e19\u4e8c\u919b\uff08MDA\uff09\u3001\u603b\u6297\u6c27\u5316\u80fd\u529b\uff08T-AOC\uff09\u6c34\u5e73\u3002\u7ed3\u679c\u53d1\u73b0\u5728\u7edf\u8ba1\u5b66\u65b9\u9762\u5404\u7ec4\u95f4\u5fc3\u810f\u3001\u80be\u810f\u3001\u809d\u810f\u6307\u6570\u5dee\u5f02\u6ca1\u6709\u7814\u7a76\u610f\u4e49\uff0c\u540c\u6b63\u5e38\u5bf9\u7167\u7ec4\u8fdb\u884c\u6bd4\u8f83\uff0c\u6a21\u578b\u7ec4\u5c0f\u9f20\u8d28\u91cf\u589e\u52a0\u7387\u964d\u4f4e\uff0c\u813e\u810f\u6307\u6570\u964d\u4f4e\uff1b\u5c0f\u9f20\u8840\u6e05\u3001\u809d\u7ec4\u7ec7SOD\u3001GSH-Px\u3001T-AOC\u6d3b\u6027\u51cf\u5f31\uff0cMDA\u542b\u91cf\u589e\u52a0\uff1b\u5c0f\u9f20\u8111\u7ec4\u7ec7MDA\u542b\u91cf\u589e\u52a0\uff0cMAO-B\u6d3b\u6027\u589e\u5f3a\u3001SOD\u3001T-AOC\u6d3b\u6027\u51cf\u5f31\uff0c\u5dee\u5f02\u5747\u5177\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\uff08P<0.01\u6216P<0.05\uff09\u3002\u7531\u6b64\u5f97\u51fa15\u300145 mg\/kg \u5242\u91cf\u4e0b\uff0c\u8fde\u7fd8\u82f7\u5bf9\u8870\u8001\u6a21\u578b\u5c0f\u9f20\u5177\u6709\u8f83\u597d\u7684\u6297\u8870\u8001\u4f5c\u7528\u3002<\/p>\n

2.16\u6297\u9ad8\u8840\u538b\u3001\u964d\u8840\u8102<\/p>\n

\u8fde\u7fd8\u63d0\u53d6\u7269\u6709\u663e\u8457\u7684\u964d\u538b\u4f5c\u7528\u3002\u52a8\u7269\u5b9e\u9a8c\u8bc1\u660e\uff0c\u8fde\u7fd8\u63d0\u53d6\u7269\u7684\u964d\u538b\u4f5c\u7528\u8fc5\u901f\u3001\u663e\u8457\u3001\u591a\u6b21\u6ce8\u5c04\u65e0\u5feb\u901f\u8010\u53d7\u6027\uff0c\u4f46\u6301\u7eed\u65f6\u95f4\u8f83\u77ed\uff0c\u4e00\u822c\u53ef\u4f7f\u8840\u538b\u4e0b\u964d\u5230\u539f\u6c34\u5e73\u7684\u7ea650\uff05\u3002\u8fde\u7fd8\u63d0\u53d6\u7269\u5bb6\u5154\u8033\u704c\u6d41\u5b9e\u9a8c\u8bc1\u660e\uff0c\u8be5\u6ce8\u5c04\u6db2\u5bf9\u5916\u5468\u8840\u7ba1\u6709\u76f4\u63a5\u6269\u5f20\u4f5c\u7528\u3002\u5176\u964d\u4f4e\u8840\u538b\u673a\u7406\u53ef\u80fd\u662f\u901a\u8fc7\u6269\u5f20\u5916\u5468\u8840\u7ba1\u4ece\u800c\u589e\u5927\u673a\u4f53\u8840\u5bb9\u91cf\u6765\u8fbe\u5230\u964d\u8840\u538b\u76ee\u7684\u3002<\/p>\n

\u8d75\u548f\u6885\u7b49\u53d1\u73b0\uff0c\u8fde\u7fd8\u82f7\u53ef\u4ee5\u964d\u4f4e\u8425\u517b\u6027\u9ad8\u8102\u8840\u75c7\u5c0f\u9f20\u7684\u8840\u6d46\u603b\u80c6\u56fa\u9187\u3001\u7518\u6cb9\u4e09\u916f\u7b49\u6307\u6807\uff0c\u53ef\u4ee5\u5347\u9ad8\u9ad8\u5bc6\u5ea6\u8102\u86cb\u767d\u6c34\u5e73\uff0c\u6709\u6548\u964d\u4f4e\u9ad8\u8102\u8840\u75c7\u52a8\u7269\u7684\u52a8\u8109\u7ca5\u6837\u786c\u5316\u6307\u6570\uff0c\u663e\u793a\u8fde\u7fd8\u82f7\u5177\u6709\u8f83\u597d\u7684\u964d\u8840\u8102\u4f5c\u7528\u3002Kitagawa S \u7b49[60] \u53d1\u73b0\u677e\u8102\u7d20-4-O-\u03b2-D-\u5421\u5583\u8461\u8404\u7cd6\u82f7\u5177\u6709\u964d\u8840\u538b\u7684\u4f5c\u7528\u3002<\/p>\n

2.17\u6297\u75b2\u52b3\u4f5c\u7528<\/p>\n

\u9ec4\u4e9a\u4e9a\u7b49\u7814\u7a76\u53d1\u73b0\uff0c\u8fde\u7fd8\u4e2d\u7684\u9ec4\u916e\u63d0\u53d6\u7269\u5bf9\u529b\u7aed\u6e38\u6cf3\u5c0f\u9f20\u809d\u810f\u3001\u8102\u8d28\u8fc7\u6c27\u5316\u4ea7\u7269MDA\u542b\u91cf\u53ca\u8d85\u5fae\u7ed3\u6784\u3001\u5fc3\u808c\u548c\u80a1\u56db\u5934\u808c\u7684SOD\u6d3b\u6027\u5177\u6709\u91cd\u8981\u7684\u5f71\u54cd\uff0c\u901a\u8fc7\u8c03\u8282\u808c\u8089\u7ec4\u7ec7\u76f8\u5173\u9176\u542b\u91cf\u53ca\u6d3b\u6027\u6765\u8fbe\u5230\u6539\u5584\u5c0f\u9f20\u7684\u6d3b\u52a8\u673a\u80fd\uff0c\u4ece\u800c\u8bc1\u660e\u8fde\u7fd8\u5177\u6709\u6297\u75b2\u52b3\u4f5c\u7528\u3002\u4faf\u6539\u971e\u7b49\u901a\u8fc7\u52a8\u7269\u5b9e\u9a8c\u8bc1\u660e\uff0c\u8fde\u7fd8\u53f6\u63d0\u53d6\u7269\u53ef\u4ee5\u63d0\u9ad8\u529b\u7aed\u72b6\u6001\u4e0b\u5c0f\u9f20\u8111\u7ec4\u7ec7SOD\u548cPOD\u6d3b\u6027\uff0c\u964d\u4f4e\u529bMDA\u7684\u542b\u91cf\u53ca\u4e73\u9178\u8131\u6c22\u9176(LDH)\u540c\u5de5\u9176\u6d3b\u6027\uff0c\u63d0\u9ad8\u8fd0\u52a8\u540e\u6062\u590d\u72b6\u6001\u4e0b\u5c0f\u9f20\u8111\u7ec4\u7ec7LDH4\u30015\u7684\u6d3b\u6027\uff0c\u8fde\u7fd8\u53f6\u63d0\u53d6\u7269\u5bf9\u529b\u7aed\u8fd0\u52a8\u72b6\u6001\u4e0b\u7684\u8111\u7ec4\u7ec7\u6709\u4fdd\u62a4\u4f5c\u7528\uff0c\u53ef\u5ef6\u7f13\u8fd0\u52a8\u4e2d\u67a2\u7cfb\u7edf\u7684\u75b2\u52b3\u3002<\/p>\n

2.18 \u5bf9\u963f\u5c14\u8328\u6d77\u9ed8\u75c5(AD)\u52a8\u7269\u6a21\u578b\u5b66\u4e60\u8bb0\u5fc6\u7684\u6539\u5584\u4f5c\u7528<\/p>\n

\u674e\u957f\u7984\u7b49\u7814\u7a76\u8fde\u7fd8\u916f\u82f7\u5bf9\u963f\u5c14\u8328\u6d77\u9ed8\u75c5(AD)\u52a8\u7269\u6a21\u578b\u5b66\u4e60\u8bb0\u5fc6\u7684\u6539\u5584\u4f5c\u7528\uff0c\u8fdb\u884c\u8fde\u7fd8\u916f\u82f7\u4f5c\u4e3a\u6cbb\u7597AD\u7684\u6210\u836f\u6027\u521d\u6b65\u7814\u7a76\u3002\u65b9\u6cd5\uff1a\u9009\u752812\u6708\u9f84\u81ea\u7136\u8870\u8001\u6606\u660e\u79cd\u96c4\u6027\u5c0f\u9f20\uff0c\u4fa7\u8111\u5ba4\u6ce8\u5c04A\u03b2\uff1a\u5efa\u7acbAD\u52a8\u7269\u6a21\u578b\u3002\u672f\u540e10d\u5f00\u59cb\u8fdb\u884c\u884c\u4e3a\u5b66\u68c0\u6d4b\uff0c\u89c2\u5bdf\u5c0f\u9f20\u5b66\u4e60\u8bb0\u5fc6\u7684\u53d8\u5316\uff1b\u68c0\u6d4b\u8111\u7ec4\u7ec7\u6d77\u9a6c\u548c\u76ae\u5c42\u4e59\u9170\u80c6\u78b1\u916f\u9176\u3001\u4e59\u9170\u80c6\u78b1\u8f6c\u79fb\u9176\u3001\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\u7684\u6d3b\u529b\u4ee5\u53ca\u76ae\u5c42\u4e2d\u5355\u80fa\u6c27\u5316\u9176\u7684\u6d3b\u529b\u548c\u4e19\u4e8c\u919b\u7684\u542b\u91cf\u3002\u7ed3\u679c\uff1a\u5728\u6c34\u8ff7\u5bab\u5b9e\u9a8c\u4e2d\u8fde\u7fd8\u916f\u82f7\u80fd\u660e\u663e\u6539\u5584\u6a21\u578b\u52a8\u7269\u5b66\u4e60\u8bb0\u5fc6\u80fd\u529b\uff1b\u540c\u65f6\u8fde\u7fd8\u916f\u82f7\u80fd\u964d\u4f4e\u6a21\u578b\u52a8\u7269\u8111\u7ec4\u7ec7\u6d77\u9a6c\u548c\u76ae\u5c42\u4e59\u9170\u80c6\u78b1\u916f\u9176\u6d3b\u529b\uff0c\u5347\u9ad8\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\u548c\u4e59\u9170\u80c6\u78b1\u8f6c\u79fb\u9176\u6d3b\u529b\uff0c\u5e76\u4e14\u964d\u4f4e\u4e86\u76ae\u5c42\u4e2d\u4e19\u4e8c\u919b\u542b\u91cf\u548c\u5355\u80fa\u6c27\u5316\u9176\u7684\u6d3b\u529b\u3002\u7ed3\u8bba\uff1a\u8fde\u7fd8\u916f\u82f7\u5177\u6709\u6539\u5584\u62dfAD\u52a8\u7269\u6a21\u578b\u5b66\u4e60\u8bb0\u5fc6\u969c\u788d\u7684\u4f5c\u7528\u3002<\/p>\n

2.19\u514d\u75ab\u8c03\u8282\u4f5c\u7528 <\/p>\n

\u4e8e\u6653\u4e1c\u7b49\u7814\u7a76\u4e86\u8fde\u7fd8\u82f7\u5bf9\u73af\u78f7\u9170\u80fa\u5904\u7406\u5c0f\u9f20\u514d\u75ab\u529f\u80fd\u7684\u5f71\u54cd\u3002\u901a\u8fc7\u8179\u8154\u6ce8\u5c04\u73af\u78f7\u9170\u80fa\u5efa\u7acb\u5c0f\u9f20\u7684\u514d\u75ab\u6291\u5236\u6a21\u578b\uff0c\u540c\u65f6\u8bbe\u7acb\u6a21\u578b\u7ec4\u548c\u5bf9\u7167\u7ec4\uff0c\u7528\u4e0d\u540c\u5242\u91cf\u8fde\u7fd8\u82f7\u548c\u7b49\u4f53\u79ef\u751f\u7406\u76d0\u6c34\u5206\u522b\u704c\u80c3\uff0c\u5206\u6790\u4e0d\u540c\u5242\u91cf\u7684\u8fde\u7fd8\u82f7\u5bf9\u514d\u75ab\u6291\u5236\u5c0f\u9f20\u80f8\u817a\u548c\u813e\u7684\u810f\u5668\u7cfb\u6570\u3001\u8179\u8154\u5de8\u566c\u7ec6\u80de\u541e\u566c\u529f\u80fd\u3001\u5916\u5468\u8840IL\uff0d2\u3001IL\uff0d4\u548c\u5916\u5468\u8840\u6dcb\u5df4\u7ec6\u80dec AMP\u542b\u91cf\u7b49\u6307\u6807\u7684\u5f71\u54cd\u3002\u7ed3\u679c\u8868\u660e\uff0c\u8fde\u7fd8\u82f7\u80fd\u591f\u62b5\u6297\u73af\u78f7\u9170\u80fa\u7684\u514d\u75ab\u6291\u5236\u4f5c\u7528\uff0c\u5177\u6709\u4e00\u5b9a\u7684\u514d\u75ab\u8c03\u8282\u529f\u80fd\u3002<\/p>\n

2.20 \u5176\u4ed6\u4f5c\u7528<\/p>\n

\u8fde\u7fd8\u8fd8\u6709\u6539\u5584\u7259\u5468\u75c7\u72b6\u3001\u4fdd\u62a4\u5fc3\u810f\u635f\u4f24\u7b49\u4f5c\u7528\u3002\u82cf\u5a1f\u5a1f\u7b49\u5efa\u7acb\u7259\u5468\u708e\u5927\u9f20\u6a21\u578b\uff0c\u5229\u7528HE\u67d3\u8272\u3001\u86cb\u767d\u514d\u75ab\u5370\u8ff9\u68c0\u6d4b\u7b49\u65b9\u6cd5\u63a2\u8ba8\u8fde\u7fd8\u82f7\u5bf9\u7259\u5468\u708e\u5927\u9f20p38\u4e1d\u88c2\u539f\u6d3b\u5316\u86cb\u767d\u6fc0\u9176\/ c\uff0dFos\u4fe1\u53f7\u901a\u8def\u53ca\u7834\u9aa8\u7ec6\u80de\u6d3b\u5316\u7684\u5f71\u54cd\u3002\u7814\u7a76\u8868\u660e\u8fde\u7fd8\u82f7\u53ef\u4e0b\u8c03p38MAPK\/c\uff0dFos\u4fe1\u53f7\u901a\u8def\uff0c\u6291\u5236\u7259\u5468\u708e\u75c7\u53ca\u7834\u9aa8\u7ec6\u80de\u6d3b\u5316\uff0c\u51cf\u8f7b\u7259\u5468\u7ec4\u7ec7\u635f\u4f24\uff0c\u6539\u5584\u7259\u5468\u708e\u5927\u9f20\u75c7\u72b6\u3002<\/p>\n

\u8fd8\u80fd\u7528\u4e8e\u6cbb\u7597\u6025\u6027\u80be\u708e\u3001\u80ba\u7ed3\u6838\u3001\u89c6\u7f51\u819c\u9ec4\u6591\u533a\u51fa\u8840\u3001\u5443\u9006\u3001\u4fbf\u79d8\u7b49\u3002<\/p>\n

3. \u4e34\u5e8a\u5e94\u7528<\/p>\n

3.1 \u8db3\u90e8\u6e83\u75a1<\/p>\n

\u5170\u6d77\u6885\u5bf92003~2005\u5e74\u786e\u8bca\u5c0f\u817f\u6162\u6027\u6e83\u75a1\u7684\u95e8\u8bca\u6216\u4f4f\u9662\u60a3\u8005\uff0c\u5176\u4e2d\u7537\u602729\u4f8b\uff0c\u5973\u602725\u4f8b\uff0c\u5e74\u9f8415~78\u5c81\uff0c\u5e73\u574758\u5c81\uff0c\u75c5\u7a0b3\u4e2a\u6708~12\u5e74\u3002\u6309\u75c5\u56e0\u5206\u7c7b\uff0c\u5760\u79ef\u6027\u6e83\u75a1\u800521\u4f8b\uff0c\u7cd6\u5c3f\u75c5\u6027\u6e83\u75a1\u80056\u4f8b\uff0c\u767d\u8840\u75c5\u5408\u5e76\u5916\u4f24\u5f15\u8d77\u80052\u4f8b\uff0c\u7cfb\u7edf\u6027\u7ea2\u6591\u72fc\u75ae\u5408\u5e76\u8840\u7ba1\u708e\u5f15\u8d77\u80058\u4f8b\uff0c\u5916\u4f24\u611f\u67d3\u800512\u4f8b\uff0c\u7ed3\u8282\u6027\u52a8\u8109\u708e\u6240\u81f4\u80053\u4f8b\u3001\u786c\u76ae\u75c5\u5408\u5e76\u5c0f\u817f\u6e83\u75a1\u80052\u4f8b\u6309\uff1b\u9762\u79ef\u5206\u7c7b\uff0c\u9762\u79ef<3cm2<\/sup>\u800533\u4f8b\uff0c3~6cm2<\/sup>\u800514\u4f8b\uff0c\u53cd\u8005\u4f8b\u6309\u90e8\u4f4d\u5206\u7c7b\uff0c\u5355\u4fa7\u80a2\u4f53\u800539\u4f8b\uff0c\u53cc\u4fa7\u80a2\u4f53\u800515\u4f8b\u3002\u6cbb\u7597\u65b9\u6cd5\u4ee5\u8346\u82a5\u8fde\u7fd8\u6c64\u4e3a\u57fa\u672c\u65b9\uff08\u8346\u82a5\u3001\u8fde\u7fd8\u3001\u767d\u82b7\u3001\u751f\u5730\u3001\u767d\u828d\u3001\u5f53\u5f52\u3001\u5ddd\u828d\u3001\u7518\u8349\u3001\u67f4\u80e1\u3001\u6854\u6897\u3001\u9632\u98ce\u3001\u67b3\u58f3\u3001\u8584\u8377\u3001\u9ec4\u8fde\u3001\u9ec4\u67cf\u3001\u6800\u5b50\u3001\u9ec4\u82aa\u5171254g\uff09\uff0c\u6bcf\u65e5\u4e00\u5242\uff0c\u6c34\u714e30\u5206\u949f\uff0c\u836f\u91cf\u89c6\u75c5\u53d8\u90e8\u4f4d\u800c\u5b9a\uff0c\u667e\u6e29\u540e\u6d78\u6ce1\u75c5\u53d8\u90e8\u4f4d\uff0c\u6bcf\u6b21120\u5206\u949f\uff0c\u4e00\u65e5\u4e24\u6b21\uff0c\u6d78\u6ce1\u540e\u66b4\u9732\u60a3\u5904\uff0c15\u5929\u4e3a1\u7597\u7a0b\u3002\u7ed3\u679c:21\u4f8b\u5760\u79ef\u6027\u6e83\u75a1\uff0c\u6cbb\u61086\u4f8b\uff0c\u663e\u654811\u4f8b\uff0c\u6709\u65484\u4f8b\u30026\u4f8b\u7cd6\u5c3f\u75c5\u6027\u6e83\u75a1\uff0c\u663e\u65482\u4f8b\uff0c\u6709\u65483\u4f8b\u30022\u4f8b\u8840\u6db2\u75c5\u6240\u81f4\u5c0f\u817f\u6e83\u75a1\uff0c\u6cbb\u61081\u4f8b\uff0c\u663e\u65481\u4f8b\u30028\u4f8bSLE\u5408\u5e76\u8109\u7ba1\u708e\uff0c\u663e\u65484\u4f8b\uff0c\u6709\u65483\u4f8b\u300212\u4f8b\u5916\u4f24\u611f\u67d3\u6027\u6e83\u75a1\uff0c\u6cbb\u61085\u4f8b\uff0c\u663e\u65484\u4f8b\uff0c\u6709\u65483\u4f8b\u30023\u4f8b\u7ed3\u8282\u6027\u52a8\u8109\u708e\u5f15\u8d77\u5c0f\u817f\u6e83\u75a1\uff0c\u663e\u65481\u4f8b\uff0c\u6709\u65482\u4f8b\u30022\u4f8b\u786c\u76ae\u75c5\u5408\u5e76\u5c0f\u817f\u6e83\u75a1\uff0c\u6709\u65481\u4f8b\u3002\u8346\u82a5\u8fde\u7fd8\u6c64\u6d78\u6ce1\u6cbb\u759754\u4f8b\u5c0f\u817f\u6e83\u75a1\uff0c\u603b\u6709\u6548\u7387\u8fbe94.4%\u3002\u7ecf\u8fc730\u5929\u6cbb\u7597\uff0c\u5176\u6cbb\u6108\u738759%\uff0c\u663e\u6548\u738791%\uff0c\u603b\u6709\u6548\u7387\u8fbe100%\u672a\u89c1\u660e\u663e\u4e0d\u826f\u53cd\u5e94\u3002\u5728\u6cbb\u7597\u8fc7\u7a0b\u4e2d\uff0c\u5747\u65e0\u809d\u80be\u529f\u80fd\u635f\u5bb3\uff1b\u4ec52\u4f8b\u5728\u6d78\u6ce1\u6cbb\u7597\u8fc7\u7a0b\u4e2d\uff0c\u7531\u4e8e\u4e2d\u836f\u6d53\u5ea6\u8fc7\u4f4e\uff0c\u5c40\u90e8\u51fa\u73b0\u6c34\u80bf\u73b0\u8c61\uff0c\u505c\u75282\u5929\u540e\u75c7\u72b6\u6d88\u5931\u3002<\/p>\n

3.2 \u8fc7\u654f\u6027\u7d2b\u765c<\/p>\n

\u5e84\u7a0b\u658c\u62a5\u9053\uff1a\u8fde\u7fd820\u514b\uff0c\u6c34\u714e\u670d\uff0c\u8fde\u670d\u4e09\u5929\uff0c\u540c\u65f6\u5403\u5927\u67a3\uff0c\u4e0d\u9650\u91cf\u3002<\/p>\n

3.3 \u4e73\u5ca9(\u4e73\u817a\u7624)<\/p>\n

\u60a3\u8005\u5218x x \uff0c\u5973\uff0c4 6\u5c81\uff0c \u672c\u9662\u62a4\u5e08\u30021977\u5e746 \u6708\u4e2d\u65ec\u53d1\u73b0\u53f3\u4e73\u817a\u5916\u4e0a\u65b9\u6709\u4e00\u674f\u6838\u5927\u80bf\u5757\uff0c \u4e0e\u5468\u56f4\u7ec4\u7ec7\u5206\u754c\u4e0d\u6e05\uff0c \u63a8\u4e4b\u4e0d\u79fb\uff0c \u8d28\u4e2d\u7b49\u786c\u5ea6\uff0c \u897f\u533b\u7591\u4e3a\u201c \u4e73\u817a\u7ea4\u7ef4\u7624\u201d \u3002\u540c\u5e745 \u670831 \u65e5\u5728\u672c\u9662\u6458\u96643 x 2 x 2 \u5398\u7c73\u80bf\u5757(\u75c5\u7406\u5207\u7247\u53f718 8 )\u3002\u7ecf\u4e0a\u6d77\u80bf\u7624\u533b\u9662\u8bca\u65ad\u4e3a\u201c\u4e73\u817a\u5355\u7eaf\u764c\u201d\uff0c \u5317\u4eac\u80bf\u7624\u7814\u7a76\u6240\u65e5\u575b\u533b\u9662\u8bca\u65ad\u4e3a\u201c\u4e73\u817a\u6d78\u6da6\u764c\u2161\u7ea7\u201d\uff0c\u5efa\u8bae\u7acb\u5373\u8fdb\u884c\u201c\u5355\u7eaf\u6027\u4e73\u623f\u5207\u9664\u672f\u201d\u6216\u201c\u4e73\u817a\u6269\u5927\u672f\u201d\uff1b\u60a3\u8005\u4e0d\u613f\u624b\u672f\uff0c \u9042\u9080\u4f59\u8bca\u6cbb. \u73b0\u75c7\u7cbe\u795e\u6291\u90c1\uff0c \u5fe7\u8651\u82e6\u95f7\uff0c\u4e73\u623f\u80c0\u75db\uff0c \u820c\u8d28\u9eef\u7ea2\uff0c\u82d4\u8584\u767d\uff0c\u8109\u5f26\u6ed1\u800c\u6570\u3002\u6b64\u5c5e\u809d\u90c1\u6c14\u6ede\uff0c \u75f0\u70ed\u4e92\u7ed3\uff0c \u5bdf\u8840\u51dd\u7edc\uff0c\u80f6\u7ed3\u6210\u6838\u3002\u5b9c\u7406\u6c14\u5f00\u90c1\uff0c \u6e05\u70ed\u89e3\u6bd2\uff0c \u6d3b\u8840\u901a\u7edc\uff0c \u8c41\u75f0\u6563\u7ed3\u3002\u65b9\u836f\u2460: \u9999\u9644\u3001\u6d59\u8d1d\u6bcd\u3001\u5f53\u5f52\u3001\u8d64\u828d\u3001\u9648\u76ae\u3001\u738b\u4e0d\u7559\u884c\u3001\u5c71\u7532\u73e0(\u5404) 10 \u514b\uff0c \u5168\u74dc\u7bd3\u3001\u864e\u6756\u3001\u94f6\u82b1\u3001\u8fde\u7fd8\uff08\u5404) 12 \u514b\uff0c \u767d\u82b1\u86c7\u820c\u834918 \u514b. \u6c34\u714e\u670d.\u65b9\u836f\u2461 :\u9e21\u86cb7\u4e2a\uff0c \u9ec4\u814a\u5982\u849c\u74e3\u59277 \u5757\uff0c \u6bcf\u5757\u714e\u9e21\u86cb1 \u4e2a( \u4e3a1\u65e5\u91cf\uff1b1 \u6b21\u98df\u4e4b)\u3002\u8fde\u75287 \u65e5\u4e3a1 \u7597\u7a0b\u3002\u60a3\u8005\u670d\u65b9\u246090\u5242\uff0c \u836f\u540e\u7cbe\u795e\u8212\u7545\uff0c \u611f\u89c9\u826f\u597d\uff0c \u65e0\u4efb\u4f55\u4e0d\u9002\uff0c \u820c\u82d4\u3001\u8109\u8c61\u6062\u590d\u6b63\u5e38\uff0c \u4e24\u4e73\u623f\u672a\u89e6\u53ca\u80bf\u5757\uff0c\u6dcb\u5df4\u7ed3\u4e0d\u80bf\u5927\uff0c\u809d\u813e\u6b63\u5e38\uff0c 6 \u5e74\u6765\u4e00\u76f4\u575a\u6301\u5168\u65e5\u5de5\u4f5c\u3002<\/p>\n

3.4 \u7665\u7615(\u8179\u819c\u540e\u56ca\u6027\u80bf\u7624)<\/p>\n

\u738bx x \uff0c\u5973\uff0c43\u5c81\uff0c\u5de5\u4eba. \u60a3\u8005\u4e8e1981\u5e746 \u6708\u65e0\u610f\u4e2d\u53d1\u73b0\u5de6\u4fa7\u8179\u90e8\u81a8\u9686\uff0c \u7ecf\u67d0\u533b\u9662\u68c0\u67e5\u7591\u4e3a\u201c\u5375\u5de2\u56ca\u80bf\u201d\uff0c\u884c\u5256\u8179\u63a2\u67e5\uff0c\u672f\u4e2d\u8bca\u65ad\u4e3a\u201c \u8179\u819c\u540e\u56ca\u6027\u80bf\u7624\u201d\uff0c\u7531\u4e8e\u6761\u4ef6\u6240\u9650\uff0c \u4ec5\u62bd\u51fa\u5927\u91cf\u6db2\u4f53\uff0c\u672a\u5207\u9664\u80bf\u7624\u3002\u534a\u6708\u540e\u5de6\u8179\u90e8\u53c8\u89c1\u81a8\u9686\uff0c\u7ecf\u670d\u4e2d\u836f\u6cbb\u7597\u75c7\u72b6\u7f13\u89e3\uff0c10 \u5e74\u4e4b\u540e\u65e7\u75c5\u590d\u53d1\uff0c\u75c7\u72b6\u518d\u73b0\u3002\u75c5\u52bf\u9010\u6e10\u52a0\u91cd\uff0c \u8179\u90e8\u80c0\u75db\uff0c \u6076\u5fc3\u5455\u5410\uff0c \u7eb3\u5446\u5026\u6020\uff0c \u5de6\u4e0a\u8179\u89e6\u53ca\u7ea64 x 4 \u5398\u7c73\u4e4b\u80bf\u5757\uff0c\u6709\u538b\u75db. \u8d85\u58f0\u6ce2\u68c0\u67e5:\u5de6\u4e0a\u8179\u90e8\u53ef\u89c1\u76f4\u5f84\u4e3a3 x 4 \u5398\u7c73\u7684\u6db2\u5e73\u53cd\u5c04\u6ce2\u3002\u7ecf\u6570\u533b\u9662\u4f1a\u8bca\uff0c\u786e\u5b9a\u4e3a\u201c\u8179\u819c\u540e\u56ca\u6027\u80bf\u7624\u590d\u53d1\u201d\uff0c\u5efa\u8bae\u7acb\u5373\u884c\u80bf\u7624\u5207\u9664\u672f\u3002\u60a3\u8005\u4e0d\u613f\u624b\u672f\uff0c\u4e8e19\u4e866\u5e743 \u670816 \u65e5\u524d\u6765\u6c42\u6cbb\u3002\u73b0\u75c7\u8179\u90e8\u80c0\u75db\uff0c \u523a\u75db\u9635\u4f5c\uff0c \u96be\u4ee5\u5fcd\u53d7\uff0c \u98df\u6b32\u6781\u5dee\uff0c \u6cdb\u6076\u5455\u5410\uff0c \u53e3\u5e72\u559c\u51c9\uff0c\u6d88\u7626\u4e4f\u529b\uff0c \u8170\u80cc\u9178\u75db\uff0c\u6392\u4fbf\u56f0\u96be. \u8179\u90e8\u89e6\u8bca\uff0c \u53d1\u73b0\u5de6\u4e0a\u8179\u8fd1\u808b\u4e0b\u6709\u4e00\u62f3\u5934\u5927\u5305\u5757\uff0c\u8f6f\u800c\u4e0d\u575a\uff0c \u56fa\u5b9a\u4e0d\u79fb\uff0c\u538b\u75db\u660e\u663e\uff0c\u820c\u8d28\u9eef\uff0c \u82d4\u8584\u767d\uff0c\u8109\u5f26\u6570. \u6b64\u5c5e\u809d\u6c14\u90c1\u7ed3\uff0c\u6c14\u6ede\u8840\u5bee\uff0c \u8109\u7edc\u4e0d\u901a\uff0c\u79ef\u6591\u5316\u70ed. \u5b9c\u884c\u6c14\u89e3\u90c1\uff0c \u5316\u5948\u901a\u7edc\uff0c \u6e05\u70ed\u89e3\u6bd2\uff0c \u8f6f\u575a\u6d88\u75db. \u65b9\u836f: \u9999\u9644\u3001\u90c1\u91d1\u3001\u4e09\u68f1\u3001\u83aa\u672f\u3001\u8d64\u828d\u3001\u7682\u523a\u3001\u7a7f\u5c71\u7532(\u5404) 10 \u514b\uff0c \u4e39\u53c2\u3001\u7261\u86ce( \u5404) 15 \u514b\uff0c \u9cd6\u7532\u3001\u94f6\u82b1\u3001\u8fde\u7fd8\u3001\u864e\u6756\u3001\u5168\u74dc\u840e( \u5404)12\u514b\uff0c \u7ea2\u82b16 \u514b\uff0c \u767d\u82b1\u86c7\u820c\u834930 \u514b. \u6c34\u714e\u670d\uff1b\u60a3\u8005\u670d\u4e0a\u65b916 \u5242\u540e\uff0c \u98df\u6b32\u589e\u8fdb\uff0c \u6076\u5fc3\u5455\u5410\u7b49\u75c7\u72b6\u6d88\u5931\uff0c \u8179\u90e8\u80bf\u5757\u9010\u6e10\u7f29\u5c0f\u3002 1 \u65b9\u5171\u670d6 0\u5242\uff0c \u8179\u90e8\u5305\u5757\u6d88\u5931\uff0c \u8bf8\u75c7\u6d88\u9664\uff1b\u8d85\u58f0\u6ce2\u63a2\u67e5: \u672a\u53d1\u73b0\u5f02\u5e38\u6ce2\u30026 \u5e74\u540e\u8ffd\u8bbf\uff0c \u65e7\u75c5\u672a\u518d\u590d\u53d1\u3002<\/p>\n

3.5 \u653e\u5c04\u6027\u98df\u7ba1\u708e<\/p>\n

\u674e\u8302\u94a6\u7b49\u5c1d\u8bd5\u7528\u4e2d\u836f\u6cbb\u759750\u4f8b\uff0c \u6536\u5230\u826f\u597d\u6548\u679c\u300250 \u4f8b\u4e2d\u753730\u4f8b\uff0c \u597320 \u4f8b\uff0c \u98df\u7ba1\u764c30 \u4f8b\uff0c \u80ba\u764c10 \u4f8b\uff0c \u8d32\u95e8\u764c5 \u4f8b\uff0c \u7eb5\u8188\u80bf\u76245 \u4f8b\uff0c \u5e74\u9f8440\uff5e 70 \u5c81\uff0c \u4e2d\u4f4d\u5e74\u9f8458\u5c81\uff0c \u8f7b\u5ea620 \u4f8b\uff0c \u4e2d\u5ea620 \u4f8b\uff0c \u91cd\u5ea610 \u4f8b\u3002\u65b9\u6cd5\uff1a\u6839\u636e\u653e\u7597\u7597\u7a0b\u957f\u77ed\u53ca\u75c7\u72b6\u8f7b\u91cd\u5206\u8f7b\u3001\u4e2d\u3001\u91cd\u3002\u4e00\u3001\u8f7b\u5ea6\uff0c \u653e\u75971\uff5e 2 \u5468\uff0c \u98df\u7ba1\u53d7\u91cf10 \uff5e20Gy \u65f6\uff0c \u98df\u7ba1\u7c98\u819c\u51fa\u73b0\u653e\u5c04\u6027\u6c34\u80bf\u3002\u75c7\u72b6:\u53e3\u5e72\u3001\u4e0b\u54bd\u75db\uff0c \u98df\u7ba1\u764c\u8fdb\u98df\u6897\u963b\u75c7\u72b6\u8fdb\u4e00\u6b65\u52a0\u91cd\u3002\u65b9\u836f:\u91d1\u94f6\u82b110g \u3001\u8fde\u7fd810g \u3001\u7384\u53c210g \u3001\u751f\u573015g \u3001\u9ea6\u51ac10g \u3001\u5c04\u5e7210g \u3001\u5c71\u8c46\u683910g\uff0c \u98df\u6b32\u5dee\u8005\u52a0\u767d\u672f10g \u3001\u7126\u516d\u66f215g\u3001\u6c34\u714e\u670d\uff0c \u65e5\u4e00\u4ed8\u3002\u4e8c\u3001\u4e2d\u5ea6\uff0c\u653e\u75973\uff5e 4 \u5468\uff0c \u98df\u7ba1\u53d7\u91cf30 \uff5e40Gy \u65f6\uff0c \u98df\u7ba1\u7c98\u819c\u51fa\u73b0\u5c0f\u6e83\u75a1\u3002\u75c7\u72b6:\u4e0b\u54bd\u75bc\u75db\u53ca\u80f8\u9aa8\u540e\u75bc\u75db\u3002\u65b9\u836f:\u5929\u82b1\u7c8930g \u3001\u5c71\u8c46\u683915g \u3001\u9ea6\u51ac15g \u3001\u5c71\u836f30g \u3001\u91d1\u94f6\u82b110g \u3001\u8fde\u7fd810g \u3001\u84b2\u516c\u82f110g \u3001\u91ce\u83ca\u82b110g \u3001\u767d\u82a820g \u3001\u6c34\u714e\u670d\uff0c \u65e5\u4e00\u4ed8\u3002\u4e09\u3001\u91cd\u5ea6\uff0c\u968f\u7740\u5916\u7167\u5242\u91cf\u589e\u52a0\u6216\u52a0\u7528\u8154\u5185\u7167\u5c04\uff0c \u98df\u7ba1\u7c98\u819c\u6e83\u75a1\u8fdb\u4e00\u6b65\u52a0\u91cd\u3002\u75c7\u72b6:\u5267\u70c8\u4e0b\u54bd\u75db\u3001\u80f8\u80cc\u75db\u3002\u65b9\u836f:\u751f\u573015g \u3001\u7384\u53c215g\u3001\u9ea6\u51ac15g \u3001\u6c99\u53c215g \u3001\u77f3\u818f60 \uff5e 120g ( \u6253\u788e\u5148\u714e) \uff0c \u8fde\u7fd810g \u3001\u6843\u4ec110g\u3001\u4e39\u76ae10g \u3001\u94f6\u82b130g \u3001\u7518\u834910g \u3002\u52a0\u51cf:\u4f53\u5f31\u65e0\u529b\uff0c\u6c14\u77ed\uff0c \u81ea\u6c57\u8005\u52a0\u515a\u53c215g \u3001\u9ec4\u82aa30g\u3001\u8d2b\u8840\u8005\u52a0\u5f53\u5f52\uff0c \u9996\u4e4c\u540410g\uff0c\u80f8\u75db\u5267\u8005\u52a0\u5143\u80e1\uff0c\u5ddd\u695d\u5b50\u540410g\uff0c \u6076\u5fc3\u5455\u5410\u52a0\u4ee3\u8d6d\u77f330g\uff0c \u65cb\u590d\u82b110g\uff0c \u98df\u6b32\u5dee\u52a0\u795e\u66f210g\uff0c \u8c37\u9ea6\u82bd\u540430g \u3002\u7ed3\u679c\uff1a50 \u4f8b\u75c5\u4eba\u6839\u636e\u75c5\u60c5\u8f7b\u91cd\u5206\u522b\u4e88\u4ee5\u4e0a\u65b9\u836f\uff0c 1 \uff5e 2 \u5242\u663e\u6548\uff0c \u4e00\u822c\u670d5 \uff5e 10 \u4ed8\uff0c \u75c7\u72b6\u6d88\u593110 \u4f8b\uff0c \u8f6c\u8f7b35 \u4f8b\uff0c \u4e0d\u518d\u52a0\u91cd5\u4f8b\uff0c \u603b\u6709\u6548\u7387100 %\uff0c \u75c5\u4eba\u5747\u672a\u4e2d\u65ad\u653e\u7597\u3002<\/p>\n

3.6 \u6025\u6027\u75c5\u6bd2\u6027\u809d\u708e<\/p>\n

\u4ee51\u2236 1\u8fde\u7fd8\u6c34\u714e\u6db2\u7cd6\u6d46\u53e3\u670d10 mL\uff0c \u6bcf\u65e53\u6b21\uff0c 1\u6708\u4e3a1\u7597\u7a0b\uff0c\u6cbb\u7597\u6025\u6027\u75c5\u6bd2\u6027\u809d\u708e\uff0c\u5e73\u5747\u964d\u9176\u65f6\u95f4\u4e3a16. 6\u5929\uff0c\u6709\u6548\u738794. 1%\u3002\u964d\u9176\u4f5c\u7528\u8fc5\u901f\u6709\u6548\uff0c\u6b64\u4f5c\u7528\u4e0e\u8fde\u7fd8\u7684\u6e05\u70ed\u89e3\u6bd2\u3001\u6d88\u80bf\u6563\u7ed3\u4f5c\u7528\u6709\u5173\uff0c\u4e0e\u5176\u4fdd\u62a4\u809d\u810f\u3001\u4fc3\u8fdb\u809d\u7ec6\u80de\u518d\u751f\u3001\u964d\u4f4e\u8840\u6e05ALT\u7b49\u836f\u7406\u4f5c\u7528\u76f8\u7b26\u3002<\/p>\n

3.7 \u89c6\u7f51\u819c\u52a8\u9759\u8109\u963b\u585e<\/p>\n

\u6709\u7ef4\u8303\u7b49\u5e94\u7528\u8fde\u7fd835 g \uff0c\u52a0\u6c34600
\nm L\uff0c\u714e\u81f3450 m L\uff0c\u52063\u6b21\u996d\u524d\u670d\uff0c27\u5929\u4e3a1\u7597\u7a0b\u3002\u6cbb\u7597\u89c6\u7f51\u819c\u52a8\u9759\u8109\u963b\u585e283\u4f8b\uff0c\u5176\u4e2d\u9ec4\u6591\u533a\u51fa\u8840216\u4f8b\uff0c\u89c6\u7f51\u819c\u5176\u4ed6\u90e8\u4f4d\u51fa\u884067\u4f8b\uff0c\u4ee5\u89c6\u529b\u7684\u63d0\u9ad8\u7a0b\u5ea6\u5224\u65ad\u7597\u6548\uff0c\u7ed3\u679c\u663e\u654881\u4f8b\uff0c\u536028. 6% ; \u6709\u6548182\u4f8b\uff0c\u536064. 4% ; \u65e0\u654821\u4f8b\uff0c\u53607%\u3002\u63d0\u793a\uff0c\u8fde\u7fd8\u80fd\u4fdd\u6301\u6bdb\u7ec6\u8840\u7ba1\u6b63\u5e38\u62b5\u6297\u529b\uff0c\u964d\u4f4e\u6bdb\u7ec6\u8840\u7ba1\u7684\u8106\u6027\u548c\u901a\u900f\u6027\uff0c\u4fc3\u8fdb\u8840\u6813\u6eb6\u89e3\uff0c\u6539\u5584\u89c6\u7f51\u819c\u5c40\u90e8\u5faa\u73af\u72b6\u6001\uff0c\u4ece\u800c\u8fbe\u5230\u63d0\u9ad8\u89c6\u529b\u7684\u76ee\u7684\u3002<\/p>\n

3.8\u8fc7\u654f\u6027\u7d2b\u765c<\/p>\n

\u90d1\u5f3a\u91cd\u7528\u8fde\u7fd830\uff5e 60 g\uff0c\u4e34\u8bc1\u52a0\u51cf\u5176\u4ed6\u836f\u5473\u6cbb\u7597\u8fc7\u654f\u6027\u7d2b\u765c30\u4f8b\uff0c\u603b\u6709\u6548\u7387\u4e3a96% \uff0c\u7597\u6548\u6ee1\u610f\u3002\u63d0\u793a\uff0c\u8fde\u7fd8\u65e2\u80fd\u6e05\u8840\u70ed\uff0c\u53c8\u80fd\u6563\u7ed3\u6d88\u6591\uff0c\u5bf9\u6bdb\u7ec6\u8840\u7ba1\u7834\u88c2\u51fa\u8840\u3001\u76ae\u4e0b\u51fa\u8840\u4e4b\u8fc7\u654f\u6027\u7d2b\u765c\u6709\u9632\u6cbb\u4f5c\u7528\u3002<\/p>\n

3.9\u624b\u6307\u62d8\u6025<\/p>\n

\u91d1\u666e\u653e\u4e8e\u8fa8\u8bc1\u65b9\u4e2d\u52a0\u8fde\u7fd812\uff5e 15 g\uff0c\u8bd5\u7528\u4e8e\u591a\u79cd\u539f\u56e0\u5f15\u8d77\u7684\u624b\u6307\u62d8\u6025\uff0c\u7597\u6548\u8f83\u597d\u30022\u4f8b\u4e0d\u540c\u539f\u56e0\u5f15\u8d77\u7684\u624b\u6307\u62d8\u6025\u60a3\u8005\uff0c\u5728\u670d\u7528\u8fa8\u8bc1\u65b9\u65e0\u6548\u7684\u60c5\u51b5\u4e0b\uff0c\u5728\u539f\u65b9\u4e2d\u52a0\u8fde\u7fd815 g \uff0c\u6839\u636e\u75c5\u60c5\u8fde\u670d5\u5242\u621610\u5242\uff0c\u75c7\u72b6\u5927\u51cf\uff0c\u518d\u670d20\u5242\uff0c\u75c7\u72b6\u6d88\u5931\u3002\u63d0\u793a\uff0c\u8fde\u7fd8\u5347\u6d6e\u5ba3\u6563\uff0c\u80fd\u6d41\u901a\u6c14\u8840\uff0c\u6cbb\u5341\u4e8c\u7ecf\u8840\u51dd\u6c14\u805a\u3002\u800c\u624b\u6307\u62d8\u6025\u603b\u5c5e\u7600\u963b\u8109\u7edc\uff0c\u6c14\u8840\u4e0d\u7545\uff0c\u6545\u7528\u8fde\u7fd8\uff0c\u53ef\u8c13\u836f\u4e2d\u75c5\u673a\u3002<\/p>\n

3.10\u8840\u6dcb<\/p>\n

\u674e\u79cb\u8d35\u62a5\u9053\u91cd\u7528\u8fde\u7fd820\uff5e 45 g\uff0c\u53ef\u7528\u4e8e\u6025\u6027\u80be\u708e\u3001\u80be\u76c2\u80be\u708e\u3001\u6ccc\u5c3f\u7cfb\u611f\u67d3\u53ca\u539f\u56e0\u4e0d\u660e\u4e4b\u8840\u5c3f\u3002\u4e00\u822c\u4f7f\u7528\u8fde\u7fd830 g \u4ee5\u4e0a\uff0c\u714e\u6c64150 m L\uff0c\u52063\u6b21\u6e29\u670d\uff0c\u5c0f\u513f\u914c\u51cf\uff0c\u8fde\u752810\u5929\uff0c\u53ef\u83b7\u663e\u6548\uff0c\u4e14\u53ef\u6d88\u9664\u5c3f\u86cb\u767d\u548c\u6d88\u80bf\u3002\u6b64\u4f5c\u7528\u4e0e\u5176\u6e05\u8840\u5206\u4e4b\u70ed\u3001\u901a\u6dcb\u4f5c\u7528\u6709\u5173\uff0c\u4e0e\u5176\u6297\u83cc\u3001\u6291\u83cc\u3001\u5229\u5c3f\u7b49\u73b0\u4ee3\u836f\u7406\u4f5c\u7528\u76f8\u7b26\u3002<\/p>\n

3.11\u5455\u5410<\/p>\n

\u4f55\u8fd0\u5f3a\u6cbb\u7597\u4e0d\u8bba\u4f55\u79cd\u539f\u56e0\u5f15\u8d77\u7684\u5455\u5410\uff0c\u5728\u8fa8\u8bc1\u65bd\u6cbb\u7684\u57fa\u7840\u4e0a\u52a0\u7528\u8fde\u7fd8\uff0c\u90fd\u6709\u6548\u679c\u3002\u795e\u7ecf\u6027\u5455\u5410\u60a3\u8005\uff0c\u5355\u5473\u8fde\u7fd860g \u6c34\u714e\u670d\uff0c\u6b62\u5455\u4f5c\u7528\u663e\u8457\u3002\u98df\u540e\u5455\u5410\u60a3\u8005\uff0c\u7528\u8fa8\u8bc1\u65bd\u6cbb\u6548\u679c\u4e0d\u663e\u65f6\uff0c\u4e8e\u539f\u65b9\u4e2d\u52a0\u7528\u8fde\u7fd8\u5373\u53ef\u53d6\u6548\u3002\u6b64\u4f5c\u7528\u4e0e\u5176\u9547\u5455\u6b62\u5410\u7684\u836f\u7406\u4f5c\u7528\u76f8\u7b26\u3002<\/p>\n

3.12\u5443\u9006<\/p>\n

\u738b\u4e4b\u70b3\u7528\u7092\u8fde\u7fd8\u5fc3\u6cbb\u7597\u4e0d\u540c\u539f\u56e0\u5f15\u8d77\u7684\u5443\u9006\u5747\u6709\u6ee1\u610f\u7597\u6548\u3002\u5bd2\u8bc1\u6216\u70ed\u8bc1\u7684\u5443\u9006\u60a3\u8005\uff0c\u7528\u8fde\u7fd8\u5fc360g \uff0c\u5206\u522b\u7092\u7126\u3001\u7092\u9ec4\u714e\u6c34\uff0c 1\u5242\u5373\u6108\u30021\u4f8b\u60a3\u8005\u65f6\u4f5c\u5443\u9006\u534a\u5e74\uff0c\u7528\u8fde\u7fd8\u5fc3120g \uff0c\u7092\u7126\u4e3a\u672b\uff0c\u65e5\u670d3\u6b21\uff0c\u6bcf\u6b2110g \uff0c\u836f\u5b8c\u75c5\u9664\u3002\u5176\u673a\u7406\u6709\u5f85\u8fdb\u4e00\u6b65\u7814\u7a76\u3002<\/p>\n

3.13\u80ba\u7ed3\u6838<\/p>\n

\u5355\u7528\u8fde\u7fd8\u6cbb\u7597\u80ba\u7ed3\u6838\u6709\u6291\u5236\u75c5\u53d8\u6e17\u51fa\u53ca\u6bd2\u7d20\u7684\u518d\u5438\u6536\u4f5c\u7528\uff0c\u5e76\u6709\u6b62\u8840\u6548\u679c\u3002<\/p>\n

3.14\u514d\u75ab\u75c5<\/p>\n

\u5355\u7528\u5927\u5242\u91cf\u8fde\u7fd8\u6c34\u714e\u670d\uff0c\u53ef\u7528\u4e8e\u5404\u79cd\u514d\u75ab\u75c5\u6025\u6027\u6d3b\u52a8\u671f\u6709\u9ad8\u70ed\u75c7\u72b6\u8005\uff0c\u5177\u6709\u660e\u663e\u7684\u6297\u708e\u3001\u6291\u5236\u514d\u75ab\u53cd\u5e94\u3001\u9000\u70ed\u7b49\u4f5c\u7528\u3002<\/p>\n

3.15 \u6025\u6027\u80be\u5c0f\u7403\u80be\u708e<\/p>\n

\u8499\u606f\u679d\u5206\u6790\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u52a0\u51cf\uff08\uff09\u6cbb\u7597\u6025\u6027\u80be\u5c0f\u7403\u80be\u708e\u7684\u4e34\u5e8a\u6548\u679c\u3002\u65b9\u6cd5\uff1a\u9009\u53d62016 \u5e749 \u6708\u81f32018 \u5e741 \u6708\u5e7f\u897f\u878d\u6c34\u82d7\u65cf\u81ea\u6cbb\u53bf\u4e2d\u533b\u533b\u9662\u6536\u6cbb\u7684120 \u4f8b\u6025\u6027\u80be\u5c0f\u7403\u80be\u708e\u4f5c\u4e3a\u7814\u7a76\u5bf9\u8c61\uff0c\u6309\u7167\u968f\u673a\u6570\u5b57\u8868\u6cd5\u5206\u4e3a\u89c2\u5bdf\u7ec4\u548c\u5bf9\u7167\u7ec4\uff0c\u540460 \u4f8b\u3002\u5bf9\u7167\u7ec4\u60a3\u8005\u5b9e\u65bd\u5e38\u89c4\u7684\u897f\u533b\u6cbb\u7597\uff0c\u89c2\u5bdf\u7ec4\u60a3\u8005\u5219\u5728\u5bf9\u7167\u7ec4\u7684\u6cbb\u7597\u65b9\u5f0f\u4e0a\u91c7\u7528\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u52a0\u51cf\u6cbb\u7597\u3002\u5bf9\u6bd4\u4e24\u7ec4\u60a3\u8005\u4e34\u5e8a\u6cbb\u7597\u6548\u679c\u548c\u6cbb\u7597\u540e\u7684\u4e34\u5e8a\u75c7\u72b6\u3002\u7ed3\u679c\uff1a\u89c2\u5bdf\u7ec4\u60a3\u8005\u7684\u6cbb\u7597\u603b\u6709\u6548\u7387\u4e3a95.00%\uff0c\u5bf9\u7167\u7ec4\u60a3\u8005\u7684\u6cbb\u7597\u603b\u6709\u6548\u7387\u4e3a81.67%\uff0c\u89c2\u5bdf\u7ec4\u6cbb\u7597\u603b\u6709\u6548\u7387\u660e\u663e\u9ad8\u4e8e\u5bf9\u7167\u7ec4\uff0c\u7ec4\u95f4\u6bd4\u8f83\u5dee\u5f02\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\uff08P \uff1c0.05\uff09\u3002\u540c\u65f6\uff0c\u89c2\u5bdf\u7ec4\u60a3\u8005\u7684\u6c34\u80bf\u6d88\u9000\u65f6\u95f4\u4e3a(3.67\u00b10.22)d\u3001\u8840\u5c3f\u6d88\u9000\u65f6\u95f4(2.71\u00b10.55)d\u3001\u8840\u538b\u6062\u590d\u65f6\u95f4(4.22\u00b10.67)d\uff0c\u5bf9\u7167\u7ec4\u60a3\u8005\u7684\u6c34\u80bf\u6d88\u9000\u65f6\u95f4\u4e3a\uff085.79\u00b10.48\uff09\u3001\u8840\u5c3f\u6d88\u9000\u65f6\u95f4\uff084.25\u00b10.57\uff09d\u3001\u8840\u538b\u6062\u590d\u65f6\u95f4\uff086.77\u00b10.74\uff09d\uff0c\u5404\u9879\u6307\u6807\u6d88\u9000\u65f6\u95f4\u6bd4\u8f83\u89c2\u5bdf\u7ec4\u660e\u663e\u5feb\u4e8e\u5bf9\u7167\u7ec4\uff08P \uff1c 0.05\uff09\u3002\u7ed3\u8bba\uff1a\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u52a0\u51cf\u6cbb\u7597\u6025\u6027\u80be\u5c0f\u7403\u80be\u708e\u7684\u4e34\u5e8a\u6548\u679c\u663e\u8457\uff0c\u53ef\u6709\u6548\u6539\u5584\u60a3\u8005\u4e34\u5e8a\u75c7\u72b6\uff0c\u4fc3\u8fdb\u5eb7\u590d\uff0c\u5177\u6709\u975e\u5e38\u91cd\u8981\u7684\u4e34\u5e8a\u610f\u4e49\u3002<\/p>\n

\u89c2\u5bdf\u7ec4\u60a3\u8005\u5219\u5728\u5bf9\u7167\u7ec4\u7684\u6cbb\u7597\u65b9\u5f0f\u4e0a\u91c7\u7528\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u52a0\u51cf\u6cbb\u7597\uff0c\u57fa\u7840\u836f\u65b9\u4e3a\u9632\u5df1\u3001\u9ebb\u9ec4\u3001\u674f\u4ec1\u3001\u751f\u59dc\u3001\u5927\u67a3\u540410 g\uff0c\u8d64\u5c0f\u8c46\u3001\u6851\u767d\u672f\u3001\u832f\u82d3\u3001\u767d\u8305\u6839\u540412 g\uff0c\u8fde\u7fd8\u3001\u7092\u767d\u672f15 g\uff0c\u7518\u83495
\ng\uff0c\u9488\u5bf9\u4e8e\u5408\u5e76\u9ad8\u8840\u538b\u7684\u60a3\u8005\u53ef\u52a0\u5165\u5929\u9ebb\u548c\u675c\u4ef2\u540410 g\uff1b\u800c\u51fa\u73b0\u86cb\u767d\u5c3f\u60a3\u8005\u5219\u52a0\u5165\u8406\u85a210 g\uff1b\u800c\u5bf9\u4e8e\u611f\u67d3\u8f83\u4e3a\u4e25\u91cd\u7684\u60a3\u8005\uff0c\u53ef\u52a0\u5165\u84b2\u516c\u82f1\u548c\u91d1\u94f6\u82b1\u540410 g\uff1b\u8840\u5c3f\u660e\u663e\u7684\u60a3\u8005\u53ef\u52a0\u5165\u5927\u5c0f\u84df10 g\uff1b\u800c\u5bf9\u4e8e\u6d6e\u80bf\u660e\u663e\u7684\u60a3\u8005\u5219\u52a0\u5165\u8f66\u524d\u5b5010 g\uff0c\u5c06\u4e0a\u8ff0\u836f\u7269\u6c34\u714e\u716e\uff0c\u71ac\u5236\u5269\u4f59400 mL \u836f\u6db2\uff0c\u5206\u4e3a3 \u6b21\u670d\u7528\uff0c1 \u5242\/d\uff0c\u6cbb\u75974 \u5468\u3002<\/p>\n

\u4f8b\uff1a\u60a3\u8005\uff0c\u5973\uff0c15 \u5c81\u3002\u6625\u4ee4\u53d1\u75c5\uff0c\u5f00\u59cb\u9762\u7751\u6d6e\u80bf\uff0c\u5c3f\u5c11\u5fae\u54b3\uff0c\u8eab\u53d1\u4f4e\u70ed\uff0c\u54bd\u75d2\u5fae\u75db\uff0c\u7cbe\u795e\u75b2\u60eb\uff0c\u56db\u80a2\u4e4f\u529b\uff0c\u7ee7\u800c\u4e0b\u80a2\u6d6e\u80bf\uff0c\u5c3f\u6d4a\u6709\u6cab\uff0c\u5c0f\u817f\u6709\u76ae\u75b9\u5c11\u8bb8\uff0c\u820c\u7ea2\u82d4\u817b\u5fae\u9ec4\uff0c\u8109\u6d6e\u6570\u3002\u5c3f\u68c0\uff1a\u86cb\u767d(- +\uff09\uff0c\u7ea2\u7ec6\u80de(+)\uff0c\u767d\u7ec6\u80de\u5c11\u8bb8\uff0c\u9897\u7c92\u7ba1\u578b(+)\u3002\u8bca\u65ad\uff1a\u6025\u6027\u80be\u5c0f\u7403\u80be\u708e\u3002\u8fa8\u8bc1\uff1a\u758f\u98ce\u6e05\u70ed\uff0c\u5ba3\u80ba\u5229\u6c34\u3002\u65b9\u836f\uff1a\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u52a0\u5473\u3002\u7ec4\u6210\uff1a\u9ebb\u9ec46g\uff0c\u674f\u4ec112g\uff0c\u751f\u77f3\u818f30g\uff0c\u8fde\u7fd820g\uff0c\u8d64\u5c0f\u8c4630g\uff0c\u9c9c\u8305\u683940g\uff0c \u6ed1\u77f318g\uff0c \u516c\u82f130g\uff0c \u6851\u76ae20g\uff0c\u76ca\u6bcd\u834930g\uff0c\u77bf\u9ea615g\uff0c\u7518\u834910g\uff0c\u751f\u59dc15g(\u5207)\u30021.5 \u5242\/ \u65e5\uff0c\u6c34\u714e\u5206\u65e9\u5348\u665a3 \u6b21\u670d\u3002\u590d\u8bca\uff1a\u836f\u670d5 \u5242\uff0c\u5c3f\u591a\u80bf\u6d88\uff0c\u70ed\u9000\u795e\u723d\uff0c\u75b2\u4e4f\u611f\u4e5f\u6709\u6062\u590d\u3002\u590d\u67e5\u5c3f\u5e38\u89c4\uff1a\u86cb\u767d\u5fae\u91cf\uff0c\u7ea2\u767d\u7ec6\u80de\u7ba1\u578b\u6d88\u5931\u3002\u539f\u65b9\u9ebb\u9ec4\u3001\u751f\u77f3\u818f\u51cf\u91cf\uff0c\u52a0\u832f\u82d330g\uff0c \u767d\u672f15g\uff0c\u7ee7\u670d5 \u5242\uff0c\u8bf8\u75c7\u6089\u9664\u3002\u5c3d\u7ba1\u75c5\u75c7\u6062\u590d\u5982\u6b64\u4e4b\u5feb\uff0c\u5631\u5176\u95f4\u65ad\u670d\u836f\uff0c\u4e0d\u53ef\u9aa4\u505c\uff0c\u5b9a\u671f\u68c0\u67e5\uff0c\u4ee5\u9632\u53cd\u5f39\uff0c\u6050\u8f6c\u6162\u6027\u3002<\/p>\n

\u6797\u73ca\u73ca\u7b49\u91c7\u7528\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u5408\u4e94\u76ae\u996e\u65b9\uff08\u832f\u82d3\u76ae15-25 g\uff0c\u6851\u767d\u76ae8-12 g\uff0c\u5927\u8179\u76ae20-45 g\uff0c\u9648\u76ae12-18 g\uff0c\u751f\u59dc\u76ae15-20 g\uff0c\u9ebb\u9ec45-9 g\uff0c\u8fde\u7fd810-15
\ng\uff0c\u674f\u4ec18-10 g\uff0c\u6851\u767d\u76ae8-12 g\uff0c\u8d64\u5c0f\u8c4618-22 g\uff0c\u751f\u59dc4-6 g\uff0c\u5927\u67a34 \u679a\uff0c\u7518\u83496-8g\uff09\u6cbb\u7597\u6025\u6027\u80be\u708e\uff0c\u4e14\u4e34\u5e8a\u968f\u75c7\u52a0\u51cf\uff1a\u6d6e\u80bf\u91cd\u8005\u52a0\u5ddd\u725b\u819d9-12 g\uff0c\u8f66\u524d\u5b5015-20 g\uff1b\u8179\u6c34\u91cd\u8005\u52a0\u6a58\u68389-15 g\uff0c\u4e4c\u836f8-12 g\uff0c\u6728\u99994-6g\uff1b\u70e6\u70ed\u53e3\u6e34\u8005\u52a0\u77f3\u818f10-18 g\uff1b\u6e7f\u70ed\u6bd2\u90aa\u8005\u52a0\u91d1\u94f6\u82b110 -15 g\uff0c\u767d\u82b1\u86c7\u820c\u834920-25 g\uff1b\u5c3f\u86cb\u767d\u8005\u52a0\u77f3\u82c710-15 g\uff0c\u6787\u6777\u53f610-15 g\uff1b\u5c3f\u8840\u8005\u52a0\u5927\u5c0f\u84df9-12 g\uff0c\u767d\u8305\u6839 9-12 g\uff1b\u8840\u538b\u9ad8\u8005\u52a0\u675c\u4ef29-12 g\uff0c\u5929\u9ebb12-15 g\uff0c\u6bcf\u65e51 \u5242\uff0c\u6c34\u714e\u670d\uff0c14 \u5929\u4e3a1 \u4e2a\u7597\u7a0b\uff0c\u5728\u51cf\u8f7b\u6c34\u80bf\u3001\u964d\u8840\u538b\u3001\u6539\u5584\u5c11\u5c3f\u548c\u964d\u5c3f\u86cb\u767d\u4e0a\u8f83\u5e38\u89c4\u7684\u897f\u533b\u6cbb\u7597\u6709\u660e\u663e\u63d0\u9ad8\uff0c\u503c\u5f97\u5728\u4e34\u5e8a\u5e7f\u6cdb\u5e94\u7528\u3002\u8499\u5411\u6b23[3] \u7528\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\uff08\u9ebb\u9ec4\u3001\u751f\u59dc\u3001\u674f\u4ec1\u3001\u5927\u67a3\u540410 g\uff0c\u8fde\u7fd8\u3001\u8d64\u5c0f\u8c46\u3001\u9632\u5df1\u3001\u83b7\u83e9\u3001\u6851\u767d\u76ae\u3001\u767d\u8305\u6839\u3001\u7092\u767d\u672f\u540420 g\uff0c\u7518\u83495
\ng\uff09\u6cbb\u7597\u6025\u6027\u80be\u5c0f\u7403\u80be\u708e\uff0c\u6bcf\u59291 \u5242\uff0c\u6c34\u714e\u53d6\u6c41450 ml\uff0c\u6bcf\u5929\u52063 \u6b21\u6e29\u670d\uff0c\u8bc1\u660e\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u52a0\u51cf\u6cbb\u7597\u6025\u6027\u80be\u5c0f\u7403\u80be\u708e\u53ef\u63d0\u9ad8\u7684\u603b\u6cbb\u6108\u7387\u53ca\u6709\u6548\u7387\uff0c\u5728\u51cf\u8f7b\u6c34\u80bf\u3001\u964d\u4f4e\u5c3f\u7ea2\u7ec6\u80de\u548c\u5c3f\u86cb\u767d\u65b9\u9762\u8f83\u5355\u7eaf\u897f\u836f\u6cbb\u7597\u66f4\u5177\u4f18\u52bf\uff0c\u503c\u5f97\u4e34\u5e8a\u63a8\u5e7f\u548c\u5e94\u7528\u3002<\/p>\n

\u5218\u9633\u91c7\u7528\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\uff08\u9ebb\u9ec4\u3001\u674f\u4ec1\u3001\u5927\u67a3\u3001\u751f\u59dc\u540410 g\uff0c\u8d64\u5c0f\u8c46\u3001\u8fde \u7fd8\u3001\u7092\u767d\u672f\u3001\u6851\u767d\u76ae\u3001\u767d\u8305\u6839\u3001\u83b7\u83e9\u3001\u9632\u5df110 g\uff0c\u7518\u83495 g\uff09\u6cbb\u7597\u6025\u6027\u80be\u5c0f\u7403\u80be\u708e\uff0c\u968f\u8bc1\u52a0\u51cf\uff0c\u611f\u67d3\u4e25\u91cd\u8005\u52a0\u91d1\u94f6\u82b1\u3001\u84b2\u516c\u82f1\u540410
\ng\uff0c\u86cb\u767d\u5c3f\u660e\u663e\u8005\u52a0\u8349\u84b810 g\uff0c\u8840\u5c3f\u660e\u663e\u8005\u52a0\u5927\u5c0f\u84df10\uff0c\u6d6e\u80bf\u660e\u663e\u8005\u52a0\u8f66\u524d\u5b5010g\uff0c\u9ad8\u8840\u538b\u8005\u52a0\u5929\u9ebb\u3001\u675c\u4ef2\u540410 g\uff0c\u6bcf\u65e51 \u5242\uff0c\u6c34\u714e\u5206\u65e9\u4e2d\u665a3\u6b21\u6e29\u670d\u300214 \u5929\u4e3a1 \u4e2a\u7597\u7a0b\uff0c\u8fde\u75282 \u4e2a\u7597\u7a0b\uff0c\u7597\u6548\u8868\u660e\u8840\u538b\u964d\u4f4e\u65f6\u95f4\u3001\u8089\u773c\u53ca\u955c\u4e0b\u8840\u5c3f\u6d88\u9000\u65f6\u95f4\u3001\u6c34\u80bf\u6d88\u9000\u65f6\u95f4\u5747\u663e\u8457\u4f18\u4e8e\u5e38\u89c4\u897f\u836f\u6cbb\u7597\u7ec4\uff0c\u4e14\u6cbb\u7597\u540e\u8840\u808c\u9150\u6c34\u5e73\u300124 \u5c0f\u65f6\u5c3f\u86cb\u767d\u5b9a\u91cf\u53ca1\u5c0f\u65f6\u5c3f\u7ea2\u7ec6\u80de\u8ba1\u6570\u5747\u663e\u8457\u4f4e\u4e8e\u5e38\u89c4\u897f\u836f\u7ec4\u6cbb\u7597\u7ec4\u6cbb\u7597\u3002\u53ef\u89c1\uff0c\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u52a0\u51cf\u6cbb\u7597\u6025\u6027\u80be\u5c0f\u7403\u80be\u708e\u53ef\u6539\u5584\u6c34\u80bf\u3001\u8840\u5c3f\u3001\u9ad8\u8840\u538b\u7b49\u4e34\u5e8a\u75c7\u72b6\uff0c\u7597\u6548\u660e\u663e\u4f18\u4e8e\u5e38\u89c4\u897f\u533b\u7ec4\u6cbb\u7597\u3002<\/p>\n

3.16 \u98ce\u75b9<\/p>\n

\u60a3\u8005\uff0c\u7537\uff0c29 \u5c81\u3002\u5916\u51fa\u52a9\u5de5\uff0c\u52b3\u4f5c\u6c57\u51fa\uff0c\u8eab\u611f\u75b2\u4e4f\uff0c\u665a\u95f4\u4ee5\u5bb4\u9152\u62db\u5f85\u3002\u591c\u534a\uff0c\u8eab\u8d77\u98ce\u75b9\u5757\u7619\u75d2\uff0c\u76ae\u80a4\u7ea2\u6da6\uff0c\u5168\u8eab\u53d1\u70ed\uff0c\u54bd\u75d2\uff0c\u5598\u6c14\u5589\u6709\u618b\u95f7\u611f\uff0c\u53e3\u70e6\u6e34\uff0c\u5c3f\u8d64\u820c\u7ea2\uff0c\u8109\u6d6e\u6ed1\u7a0d\u6570\u3002\u8fa8\u8bc1\uff1a\u98ce\u75b9\u7619\u75d2\uff0c\u70ed\u90c1\u808c\u8868\u3002\u6cbb\u6cd5\uff1a\u89e3\u8868\u9000\u70ed\uff0c\u758f\u98ce\u6b62\u75d2\u3002\u65b9\u836f\uff1a\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u52a0\u5473\u3002\u7ec4\u6210\uff1a\u9ebb\u9ec45g\uff0c\u8fde\u7fd830g\uff0c\u674f\u4ec112g\uff0c\u8346\u82a515g\uff0c\u9632\u98ce12g\uff0c\u751f\u77f3\u818f20g\uff0c\u6ed1\u77f315g\uff0c\u767d\u9c9c\u76ae20g\uff0c\u5730\u80a4\u5b5030g\uff0c \u5929\u866b15g\uff0c \u8d64\u5c0f\u8c4630g\uff0c \u751f\u59dc15g\u30021 \u5242\/ \u65e5\uff0c\u6c34\u714e\u5206\u65e9\u665a2 \u6b21\u670d\u3002\u590d\u8bca\uff1a\u836f\u670d4 \u5242\uff0c\u70ed\u9000\u8eab\u51c9\uff0c\u76ae\u75b9\u6d88\u9000\uff0c\u70e6\u6e34\u5df2\u9664\uff0c\u8109\u4e0d\u6d6e\u6ed1\u800c\u8f6c\u865a\u7f13\u3002\u4e3a\u6839\u6cbb\u4e0d\u53cd\u5f39\uff0c\u7ee7\u670d\u4e0a\u65b9\u53bb\u77f3\u818f\u3001\u6ed1\u77f3\uff0c\u52a0\u5f53\u5f5215g\uff0c\u8d64\u767d\u828d\u540415g\uff0c4 \u5242\u6108\u3002<\/p>\n

3.17\u9ec4\u75b8<\/p>\n

\u6797\u67d0\u67d0\uff0c\u7537\uff0c50\u5c81\u30022\u65e5\u524d\u60a3\u8005\u6076\u5bd2\uff0c \u53d1\u70ed38.2\u2103\uff0c\u7ee7\u5219\u9762\u76ee\u53d1\u9ec4\uff0c\u80a2\u4f53\u762b\u8f6f\uff0c\u53e3\u817b\uff0c\u538c\u98df\uff0c\u5c3f\u9ec4\uff0c\u820c\u7ea2\u82d4\u5fae\u817b\uff0c\u8109\u6d6e\u6fe1\uff0c\u7ecf\u809d\u529f\u80fd\u5316\u9a8c\u8c37\u4e19\u8f6c\u6c26\u9176116u\/L\uff0c\u603b\u80c6\u7ea2\u7d2032umol\/L\u3002\u8bca\u4e3a\u6025\u6027\u9ec4\u75b8\u809d\u708e\u3002\u8bc1\u5c5e\uff1a\u5916\u611f\u5bd2\u90aa\uff0c\u91cc\u6709\u6e7f\u70ed\u3002\u6cbb\u5b9c\u89e3\u8868\uff0c\u6e05\u70ed\u9664\u6e7f\u9000\u9ec4\u3002\u836f\u7528\u9ebb\u9ec46g\uff0c \u8fde\u7fd810g\uff0c \u8d64\u5c0f\u8c4615g\uff0c\u674f\u4ec110g\uff0c\u677f\u84dd\u683915g\uff0c\u8335\u964830g\uff0c\u751f\u59dc3 \u7247\uff0c\u7518\u83495g\uff0c \u82cd\u672f10g\uff0c 3\u5242\u540e\uff0c\u8868\u8bc1\u5df2\u89e3\uff0c\u9762\u76ee\u9ec4\u9000\uff0c \u7eb3\u589e\uff0c\u7ee7\u4ee5\u6e05\u70ed\u5229\u6e7f\u8c03\u7406\u800c\u6108\u3002\u6309\uff1a\u6b64\u8bc1\u5b8c\u5168\u4e0e\u5f20\u4ef2\u666f\u6240\u8bbe\u75c7\u72b6\u76f8\u540c\uff0c\u65bd\u4ee5\u65b9\u836f\uff0c\u4e00\u51fb\u800c\u4e2d\uff0c\u53ef\u89c1\u4f24\u5bd2\u7acb\u65b9\u836f\u4e4b\u7cbe\u5bc6\uff0c \u4e8e\u540e\u4e16\u5177\u6709\u6df1\u8fdc\u5f71\u54cd\u3002<\/p>\n

3.18\u75e4\u75ae<\/p>\n

\u738b\u8273\u5b8f\u89c2\u5bdf\u8346\u9632\u8fde\u7fd8\u6c64\u52a0\u51cf\u6cbb\u7597\u75e4\u75ae\u6548\u679c\u3002\u65b9\u6cd5\uff1a\u9009\u53d62017\u5e749\u67081\u65e5\uff5e2018\u5e7410\u670831\u65e5\u6211\u9662\u6536\u6cbb\u7684\u6bdb\u56ca\u708e\u60a3\u800580\u4f8b\u4f5c\u4e3a\u7814\u7a76\u5bf9\u8c61\uff0c\u6309\u7167\u968f\u673a\u6570\u5b57\u6cd5\u5c06\u5176\u5206\u4e3a\u4e24\u7ec4\uff0c\u540440\u4f8b\u3002\u6cbb\u7597\u7ec4\u4ee5\u8346\u9632\u8fde\u7fd8\u6c64\u52a0\u51cf\u6cbb\u7597\u3002\u5bf9\u7167\u7ec4\u4ee5\u897f\u533b\u5e38\u89c4\u53bb\u8102\u3001\u6eb6\u89e3\u89d2\u8d28\u3001\u6d88\u708e\u6740\u83cc\u53ca\u8c03\u8282\u6fc0\u7d20\u6c34\u5e73\u6cbb\u7597\u3002\u7ed3\u679c\uff1a\u4e24\u7ec4\u7597\u6548\u6bd4\u8f83\uff0c\u6cbb\u7597\u7ec4\u660e\u663e\u4f18\u4e8e\u5bf9\u7167\u7ec4\uff0c\u5dee\u5f02\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\uff08P\uff1c0.05\uff09\u3002\u7ed3\u8bba\uff1a\u901a\u8fc7\u8346\u9632\u8fde\u7fd8\u6c64\u52a0\u51cf\u6cbb\u7597\u75e4\u75ae\u5bf9\u4e8e\u70ed\u6027\u4f53\u8d28\u7684\u9752\u58ee\u5e74\u53d6\u5f97\u826f\u597d\u7684\u7597\u6548\u3002\u6cbb\u7597\u65b9\u6cd5\uff1a\u4ee5\u8346\u9632\u8fde\u7fd8\u6c64\uff08\u8346\u82a5\u3001\u9632\u98ce\u3001\u6800\u5b50\u3001\u8fde\u7fd8\u3001\u67f4\u80e1\u3001\u8584\u8377\u3001\u6854\u6897\u3001\u67b3\u58f3\u3001\u767d\u82b7\u3001\u7518\u8349\u3001\u751f\u5730\u3001\u5f53\u5f52\u3001\u767d\u828d\u3001\u5ddd\u828e\u3001\u9ec4\u82a9\u3001\u9ec4\u8fde\u3001\u9ec4\u67cf\uff09\u52a0\u51cf\u6cbb\u7597\u3002\u52a0\u51cf\u6cd5\uff1a\u4f34\u6709\u7600\u8840\uff0c\u52a0\u6842\u679d\u832f\u82d3\u4e38\u3002\u4f34\u6c34\u6e7f\uff0c\u52a0\u7528\u4e94\u82d3\u6563\u3002\u4f34\u7600\u6c34\u4e92\u7ed3\uff0c\u52a0\u5f53\u5f52\u828d\u836f\u6563\u3002\u7528\u6cd5\uff1a\u6c34\u714e\u670d\uff0c\u65e5\u4e00\u5242\u7ea6150 mL\uff0c\u52062\u6b21\u670d\u7528\u3002<\/p>\n

3.19\u5916\u611f\u5598\u54b3<\/p>\n

\u5218\u67d0\u67d0\uff0c\u5973\uff0c 26\u5c81\u3002\u60a3\u8005\u7d20\u6709\u54b3\u55fd\u4e4b\u75c7\uff0c \u590d\u53d7\u5bd2\u90aa\u81f4\u6076\u5bd2\uff0c\u53d1\u70ed\uff0c \u54b3\u55fd\u6c14\u5598\uff0c\u4e0d\u5f97\u5e73\u5367\uff0c \u53e3\u6e34\uff0c\u820c\u7ea2\uff0c \u82d4\u6ed1\uff0c\u8109\u6d6e\u6570\u3002\u8bc1\u5c5e\u5916\u611f\u98ce\u5bd2\uff0c \u5185\u6709\u505c\u996e\u3002\u6cbb\u5b9c\u758f\u98ce\u6563\u5bd2\uff0c\u6cfb\u80ba\u5e73\u5598\u3002\u836f\u7528\u9ebb\u9ec45g\uff0c \u8fde\u7fd810g\uff0c \u8d64\u5c0f\u8c4615g\uff0c \u674f\u4ec110g\uff0c \u6851\u76ae10g\uff0c \u6854\u68975g\uff0c \u751f\u59dc3\u7247\uff0c \u5927\u67a35\u679a\uff0c \u8476\u82c8\u5b5015g\uff0c \u670d3\u5242\uff0c \u5598\u5e73\u800c\u5b89\uff0c \u518d\u670d\u6108\u3002\u6309\uff1a\u6b64\u8bc1\u4e3a\u5916\u611f\u98ce\u5bd2\uff0c\u5185\u6709\u505c\u996e\uff0c \u81f4\u80ba\u6c14\u5ba3\u8083\u5931\u5e38\u800c\u81f3\u5598\u54b3\u3002\u672c\u65b9\u5916\u5ba3\u80ba\u6c14\uff0c\u5185\u8083\u75f0\u996e\uff0c \u6545\u670d\u836f\u540e\u6c14\u5598\u5f97\u5e73\u3002<\/p>\n

3.20\u8368\u9ebb\u75b9<\/p>\n

\u738b\u67d0\u67d0\uff0c\u5973\uff0c 22\u5c81\u3002\u76ae\u80a4\u9690\u75b9\u6414\u75d2\uff0c \u53cd\u590d\u53d1\u4f5c\uff0c\u65f6\u9690\u65f6\u73b0\u6708\u4f59\uff0c \u53d1\u65f6\u5982\u98ce\u56e2\u6210\u7247\uff0c\u6414\u75d2\u96be\u5fcd\uff0c \u76ae\u8272\u6de1\u7ea2\uff0c\u5e76\u6709\u6076\u98ce\uff0c \u5c3f\u5c11\uff0c\u820c\u7ea2\u82d4\u767d\uff0c \u8109\u6d6e(\u66fe\u670d\u6d88\u98ce\u6563\u7b49\u836f\u65e0\u6548)\uff0c \u8bc1\u5c5e\u98ce\u6e7f\u76f8\u640f\uff0c\u90c1\u4e8e\u808c\u8868\u3002\u6cbb\u5b9c\u5916\u6563\u98ce\u90aa\uff0c \u5185\u6e05\u6e7f\u70ed\uff0c \u4ee5\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u52a0\u51cf\u6cbb\u4e4b\u3002\u836f\u7528\u9ebb\u9ec46g\uff0c \u8fde\u7fd810g\uff0c \u8d64\u5c0f\u8c4615g\uff0c \u674f\u4ec110g\uff0c \u7518\u83496g\uff0c \u6851\u53f610g\uff0c \u8749\u88636g\uff0c \u751f\u59dc3\u7247\uff0c \u858f\u82e1\u4ec115g\uff0c \u50f5\u869510g\u3002\u670d\u836f3\u5242\uff0c \u98ce\u6e7f\u9690\u9000\uff0c \u7ee7\u4ee5\u4e0a\u65b9\u52a0\u832f\u82d3\u3001\u4e39\u76ae\u670d3\u5242\uff0c \u518d\u670d6\u5242\uff0c\u672a\u518d\u590d\u53d1\u3002<\/p>\n

3.21 \u9ad8\u5c3f\u9178\u8840\u75c7<\/p>\n

\u9ec4\u4e3d\u7b49\u8ddf\u968f\u8001\u5e08\u5218\u9f99\u6c11\u6559\u6388\uff0c\u5176\u4ece\u4e8b\u4e2d\u533b\u5185\u79d1\u591a\u5e74\uff0c\u6cbb\u7597\u80be\u810f\u75c5\u6709\u5176\u72ec\u7279\u7684\u601d\u7ef4\u65b9\u5f0f\uff0c\u73b0\u5c06\u5176\u6cbb\u7597\u9ad8\u5c3f\u9178\u8840\u75c7\u7684\u4e34\u5e8a\u6848\u4f8b\u62a5\u9053\u5982\u4e0b\u3002\u9ad8\u67d0\uff0c\u7537\uff0c34\u5c81\u3002\u521d\u8bca\u65f6\u95f4\uff1a2017\u5e746\u670821\u65e5\u3002\u60a3\u8005\u56e0\u201c\u53d1\u73b0\u9ad8\u5c3f\u9178\u8840\u75c7\u534a\u5e74\uff0c\u53f3\u811a\u8f7b\u5ea6\u6d6e\u80bf3\u6708\u201d\u524d\u6765\u5c31\u8bca\uff0c\u60a3\u8005\u534a\u5e74\u524d\u65e0\u660e\u663e\u8bf1\u56e0\u51fa\u73b0\u53f3\u8db3\u80cc\u75bc\u75db\uff0c\u6765\u6211\u9662\u5c31\u8bca\uff0c\u68c0\u67e5\u53d1\u73b0\u5c3f\u9178\u504f\u9ad8\uff08\u5177\u4f53\u62a5\u544a\u4e0d\u8be6\uff09 \u672a\u4e88\u4ee5\u6b63\u89c4\u6cbb\u7597\u30023\u6708\u524d\u60a3\u8005\u65e0\u660e\u663e\u8bf1\u56e0\u51fa\u73b0\u53f3\u811a\u80cc\u6709\u8f7b\u5ea6\u6d6e\u80bf\uff0c\u538b\u4e4b\u51f9\u9677\u5373\u8d77\u3002\u5076\u6709\u75bc\u75db\uff0c\u65e0\u80f8\u95f7\u3001\u6c14\u4fc3\u3001\u65e0\u8179\u90e8\u4e0d\u9002\uff0c\u6765\u6211\u9662\u95e8\u8bca\u5c31\u8bca\uff0c\u68c0\u67e5\u53d1\u73b0\u5c3f\u9178\u504f\u9ad8\uff08\u5177\u4f53\u4e0d\u8be6\uff09\uff0c2017\u5e745\u670814\u65e5\u8840\u5c3f\u9178\uff08\u5feb\u901f\u6cd5\uff09\uff1a571.6 \u03bcmol\/L\u3002\u80be\u529f\u80fd(2017\u5e745\u670825\u65e5)\uff1a\u5c3f\u7d20\u6c2e4.1
\nmmol\/L\uff0c\u808c\u9150115 \u03bcmmol\/L\uff0c\u5e73\u5747\u8840\u7ea2\u86cb\u767d\u542b\u91cf26.6 pg\uff0c\u5e73\u5747\u7ea2\u7ec6\u80de\u4f53\u79ef81.7 fL\u3002\u65e2\u5f80\u53f2\uff1a\u5426\u8ba4\u9ad8\u8840\u538b\u3001\u7cd6\u5c3f\u75c5\u75c5\u53f2\u3001\u51a0\u5fc3\u75c5\u7b49\u6162\u6027\u75c5\u53f2\uff0c\u6709\u9ad8\u8840\u8102\u75c74\u5e74\uff0c\u5e73\u65f6\u672a\u670d\u7528\u964d\u8102\u836f\u7269\u3002\u5426\u8ba4\u70df\u9152\u53f2\u3002\u5426\u8ba4\u4e59\u809d\u3001\u7ed3\u6838\u7b49\u6162\u6027\u75c5\u53f2\u3002\u5426\u8ba4\u98df\u7269\u3001\u836f\u7269\u8fc7\u654f\u53f2\uff0c\u5426\u8ba4\u624b\u672f\u5916\u4f24\u53f2\uff0c\u5426\u8ba4\u8f93\u8840\u53f2\u3002\u523b\u4e0b\uff1a\u60a3\u8005\u76ee\u524d\u53f3\u8db3\u80cc\u75bc\u75db\uff0c\u4e0b\u80a2\u8f7b\u5ea6\u6d6e\u80bf\uff0c\u4e4f\u529b\uff0c\u591a\u6c57\uff0c\u52a8\u4e4b\u751a\u3002\u820c\u6697\u6de1\uff0c\u8109\u7ec6\u3002\u4e8c\u4fbf\u8c03\uff0c\u591c\u5bd0\u5b89\u3002\u4e2d\u533b\u8bca\u65ad\uff1a\u6c34\u80bf\u3002\u6cbb\u5219\uff1a\u6e05\u70ed\u89e3\u6bd2\uff0c\u5229\u6e7f\u6b62\u75db\u3002\u65b9\u836f\uff1a\u7099\u9ebb\u9ec46 g\uff0c\u8fde\u7fd812
\ng\uff0c\u8d64\u5c0f\u8c4620 g\uff0c\u82cd\u672f15 g\uff0c\u767d\u672f15 g\uff0c\u732a\u82d320 g\uff0c\u832f\u82d320 g\uff0c\u9e7f\u8854\u834920 g\uff0c\u8f66\u524d\u834920 g\uff0c\u8f66\u524d\u5b5015 g\uff0c\u6ed1\u77f312 g\uff0c\u9ec4\u82a910 g\uff0c\u5c71\u836f15 g\uff0c\u4e73\u99996 g\uff0c\u5ef6\u80e1\u7d2210 g\uff0c\u84b2\u516c\u82f115 g\uff0c\u751f\u7518\u83499 g\u30026\u670828\u65e5\u4e8c\u8bca\uff1a\u670d\u836f\u540e\u8bf8\u75c7\u51cf\u3002\u4ecd\u6709\u4e4f\u529b\uff0c\u591a\u6c57\uff0c\u68c0\u67e5\u793a: \u8840\u5c3f\u9178\uff08\u5feb\u901f\u6cd5\uff09\uff1a 422.8 \u03bcmol\/L\u3002\u80be\u529f\u80fd(2017\u5e746\u67083\u65e5)\uff1a\u5c3f\u7d20\u6c2e4.1
\nmmol\/L\uff0c\u808c\u9150100 \u03bcmmol\/L\uff0c\u5c3f\u9178\uff1a250 \u03bcmol\/L\u3002\u53bb\u6ed1\u77f3\uff0c\u52a0\u9ec4\u82aa15 g\uff0c\u9632\u98ce20 g\uff0c\u7ee7\u7eed\u670d\u7528\u524d\u65b9\u6cbb\u7597\u30027\u67084\u65e5\u4e09\u8bca\uff1a\u60a3\u8005\u670d\u836f\u540e\uff0c\u65e0\u7279\u6b8a\u4e0d\u9002\uff0c\u4e4f\u529b\u8f83\u524d\u6709\u6240\u597d\u8f6c\u3002\u7ee7\u7eed\u539f\u65b9\u6cbb\u7597\u3002<\/p>\n

3.22 \u6162\u6027\u80be\u708e<\/p>\n

\u8303\u5fd7\u534e\u7b49\u9009\u53d6\u5f53\u5730\u533b\u96622014 \u5e7412\u6708\u81f32015\u5e7412 \u6708\u6240\u6536\u6cbb\u7684\u6162\u6027\u80be\u708e\u60a3\u8005\u8d44\u6599100 \u4f8b\u5f00\u5c55\u56de\u987e\u6027\u5206\u6790\u4e0e\u7814\u7a76\uff0c\u7814\u7a76\u7ec450 \u4f8b\u60a3\u8005\u901a\u8fc7\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u52a0\u5473\u6cbb\u7597: \u9ebb\u9ec45 g\u3001\u751f\u59dc5 g\u3001\u8fde\u7fd815 g\u3001\u674f\u4ec110 g\u3001\u4e39\u53c210 g\u3001\u6851\u767d\u76ae10 g\u3001\u67a34 \u679a\u3001\u8d64\u5c0f\u8c4620 g\u3001\u76ca\u6bcd\u834915 g\uff0c\u6c34\u714e\u670d\uff0c\u6bcf\u59291 \u5242\uff0c\u65e9\u665a2 \u6b21\u670d\u7528\uff0c\u5bf9\u7167\u7ec450 \u4f8b\u60a3\u8005\u901a\u8fc7\u53e3\u670d\u9ec4\u8475\u80f6\u56ca\u6cbb\u7597\uff0c\u6bcf\u59293 \u6b21\uff0c\u6bcf\u6b215 \u7c92\uff1b\u7814\u7a76\u7ec4\u548c\u5bf9\u7167\u7ec4\u60a3\u8005\u5168\u90e8\u6cbb\u75973 \u4e2a\u6708\uff0c\u9488\u5bf9\u4f34\u968f\u9ad8\u8840\u538b\u3001\u7535\u89e3\u8d28\u7d0a\u4e71\u4ee5\u53ca\u4ee3\u8c22\u6027\u9178\u4e2d\u6bd2\u60a3\u8005\u91c7\u53d6\u5bf9\u75c7\u6cbb\u7597\uff0c\u7ed3\u679c\u53d1\u73b0\u7814\u7a76\u7ec450\u4f8b\u60a3\u8005\u4e2d\u4e34\u5e8a\u63a7\u523617 \u4f8b\uff0c\u663e\u654810\u4f8b\uff0c\u6709\u654812 \u4f8b\uff0c\u65e0\u65483 \u4f8b\uff0c\u6cbb\u7597\u6709\u6548\u7387\u4e3a94.0%\uff1b\u5bf9\u7167\u7ec450 \u4f8b\u60a3\u8005\u4e2d\u4e34\u5e8a\u63a7\u523610 \u4f8b\uff0c\u663e\u654810 \u4f8b\uff0c\u6709\u654812 \u4f8b\uff0c\u65e0\u654818 \u4f8b\uff0c\u6cbb\u7597\u6709\u6548\u7387\u4e3a64.0%\uff0c\u5e76\u4e14\u7814\u7a76\u7ec4\u60a3\u800524 h \u5c3f\u86cb\u767d\u5b9a\u91cf\u3001\u514d\u75ab\u7403\u86cb\u767dIgG\u3001\u8840\u6d46\u767d\u86cb\u767d\u4ee5\u53ca\u8865\u4f53C3 \u6539\u5584\u7a0b\u5ea6\u4f18\u4e8e\u5bf9\u7167\u7ec4\uff0c \u8bf4\u660e\u5bf9\u4e8e\u6162\u6027\u80be\u708e\u91c7\u53d6\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u52a0\u5473\u6cbb\u7597\uff0c\u80fd\u591f\u6539\u5584\u60a3\u8005\u4e34\u5e8a\u75c7\u72b6\uff0c\u964d\u4f4e\u5c3f\u86cb\u767d\uff0c\u63d0\u5347\u8840\u6d46\u767d\u86cb\u767d\uff0c\u5e76\u4e14\u53ef\u4ee5\u63d0\u5347\u60a3\u8005\u7684\u514d\u75ab\u80fd\u529b\u3002<\/p>\n

3.23  IgA \u80be\u75c5\u3002<\/p>\n

\u590f\u6dcb\u971e\u7b49\u91c7\u7528\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u5408\u53c2\u82aa\u5730\u9ec4\u6c64\uff08\u7099\u9ebb\u9ec4\u3001\u6851\u767d\u76ae\u3001\u7099\u7518\u8349\u3001\u5927\u67a3\u540412 g\uff0c\u8fde\u7fd8\u3001\u82e6\u674f\u4ec1\u54049g\uff0c\u8d64\u5c0f\u8c4618
\ng\uff09\u6cbb\u7597IgA \u80be\u75c5\u6c14\u9634\u4e24\u865a\u3001\u6e7f\u70ed\u517c\u6709\u8868\u8bc1\u60a3\u8005\uff0c\u6bcf\u59291 \u5242\uff0c\u6c34\u714e\u53d6\u6c41300 ml\uff0c\u5206\u65e9\u665a2 \u6b21\u6e29\u670d\uff0c\u7597\u7a0b8 \u5468\uff0c\u8bc1\u660e\u8be5\u65b9\u53ef\u6709\u6548\u63d0\u9ad8\u6cbb\u7597\u6548\u679c\uff0c\u51cf\u5c11\u5c3f\u86cb\u767d\u6392\u6cc4\uff0c\u5ef6\u7f13\u80be\u529f\u80fd\u635f\u4f24\u7684\u8fdb\u7a0b\uff0c\u540c\u65f6\u53ef\u964d\u4f4e\u8840\u6db2C3\u6c34\u5e73\uff0c\u5bf9IgA\u80be\u75c5\u7684\u9632\u6cbb\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002<\/p>\n

\u6768\u96ea\u519b\u7b49\u901a\u8fc7IgA \u80be\u75c5\u5927\u9f20\u6a21\u578b\uff0c\u7814\u7a76\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u5bf9IgA \u80be\u75c5\u5927\u9f20\u7684\u6cbb\u7597\u4f5c\u7528\u4e0e\u673a\u5236\u3002\u53d6\u9ebb\u9ec46g\u3001\u8fde\u7fd86 g\u3001\u8d64\u5c0f\u8c4615 g\u3001\u674f\u4ec19 g\u3001\u5927\u67a34 \u679a\u3001\u6851\u767d\u76ae9 g\u3001\u751f\u59dc6 g\u3001\u7518\u83496 g\uff0c\u4ee5\u6c34 500 ml \u714e\u716e\u81f350 ml\uff0c\u704c\u80c3\u3002\u7ed3\u679c\u8868\u660e\uff0c\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u80fd\u591f\u663e\u8457\u964d\u4f4eIgA \u80be\u75c5\u5927\u9f20\u7684\u5c3f\u86cb\u767d\u3001\u8840\u6e05\u808c\u9150\u53ca\u5c3f\u7d20\u6c2e\u6c34\u5e73\uff0c\u5177\u6709\u5f88\u597d\u7684\u4fdd\u62a4\u80be\u529f\u80fd\u7684\u4f5c\u7528\uff0c\u540c\u65f6\u6307\u51fa\u9ebb\u9ec4\u7684\u4e3b\u8981\u6210\u5206\u4f2a\u9ebb\u9ec4\u78b1\uff0c20 \u03bcg\/g \u7684\u7ed9\u836f\u5242\u91cf\u4e5f\u80fd\u660e\u663e\u964d\u4f4e IgA \u80be\u75c5\u5927\u9f20\u5c3f\u86cb\u767d\u3001\u8840\u6e05\u808c\u9150\u53ca\u5c3f\u7d20\u6c2e\uff0c\u63d0\u793a\u9ebb\u9ec4\u5728\u964d\u4f4eIgA \u80be\u75c5\u5927\u9f20\u7684\u5c3f\u86cb\u767d\u548c\u4fdd\u62a4\u80be\u529f\u80fd\u65b9\u9762\u4e5f\u8d77\u5230\u91cd\u8981\u4f5c\u7528\u3002<\/p>\n

3.24\u5176\u4ed6\u80be\u708e<\/p>\n

\u67f4\u745e\u9701\u6559\u6388\u8ba4\u4e3a\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u5408\u7528\u4e94\u82d3\u6563\u548c\u4e94\u76ae\u996e\uff0c\u5ba3\u80ba\u89e3\u8868\uff0c\u6e29\u9633\u5229\u6c34\uff0c\u8865\u76ca\u813e\u80be\uff0c\u5176\u5229\u6c34\u6d88\u80bf\u548c\u6e05\u9664\u5c3f\u86cb\u767d\u5c3f\u7684\u4f5c\u7528\u975e\u5e38\u660e\u663e\uff0c\u65e0\u8bba\u662f\u6025\u6027\u80be\u708e\u8fd8\u662f\u6162\u6027\u80be\u708e\uff0c\u5f53\u6c34\u80bf\u4e3a\u4e3b\u75c7\u65f6\u5373\u53ef\u7528\u672c\u65b9\uff1b\u82e5\u4e0b\u80a2\u6c34\u80bf\u4e3a\u80be\u9633\u4e0d\u8db3\u65f6\u53ef\u5408\u771f\u6b66\u6c64\u3002<\/p>\n

\u9ad8\u677e\u5bff\u6559\u6388\u91c7\u7528\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u7597\u80be\u5c0f\u7403\u80be\u708e\u5408\u5e76\u54ee\u5598\uff0c\u591a\u91c7\u7528\u6297\u611f\u67d3\u3001\u5229\u5c3f\u3001\u6297\u51dd\u6eb6\u6813\u3001\u7cd6\u76ae\u8d28\u6fc0\u7d20\u4ee5\u53ca\u900f\u6790\u7597\u6cd5\u7b49\uff0c\u9ad8\u677e\u5bff\u6559\u6388\u8fd0\u7528\u7ecf\u65b9\u8fa8\u6cbb\u80be\u5c0f\u7403\u80be\u708e\uff0c\u53d6\u5f97\u660e\u663e\u6cbb\u7597\u6548\u679c\u3002<\/p>\n

\u5434\u7389\u971e\u7b49\u91c7\u7528\u9ebb\u9ec4\u8fde\u7fd8\u8d64\u5c0f\u8c46\u6c64\u52a0\u5473\u6cbb\u7597\u75a5\u75ae\u81f4\u6025\u6027\u80be\u5c0f\u7403\u80be\u708e\uff0c\u53d1\u73b0\u8be5\u65b9\u5bf9\u6c34\u80bf\u3001\u8840\u5c3f\u3001\u86cb\u767d\u5c3f\u7f13\u89e3\u65f6\u95f4\u53ca\u75a5\u75ae\u6cbb\u6108\u65f6\u95f4\u5747\u53d6\u5f97\u4e86\u6ee1\u610f\u7684\u7597\u6548\uff0c\u4e34\u5e8a\u503c\u5f97\u5e7f\u6cdb\u5e94\u7528\u3002<\/p>\n

3.25 \u7cd6\u5c3f\u75c5\u5468\u56f4\u795e\u7ecf\u75c5\u53d8<\/p>\n

\u9ad8\u514b\u73af\u7b49\u63a2\u8ba8\u82a9\u8fde\u7fd8\u76ae\u65b9\uff08\u9ec4\u82a9\u3001\u9ec4\u8fde\u3001\u8fde\u7fd8\u3001\u6851\u767d\u76ae\u53ca\u5c71\u836f\uff09\u4f50\u6cbb\u7cd6\u5c3f\u75c5\u5468\u56f4\u795e\u7ecf\u75c5\u53d8( DPN)\u7684\u4e34\u5e8a\u7597\u6548\uff0c\u4ee5\u53ca\u5bf9\u60a3\u8005\u4e34\u5e8a\u75c7\u72b6\u548c\u795e\u7ecf\u4f20\u5bfc\u901f\u7387\u7684\u5f71\u54cd\u3002\u65b9\u6cd5\uff1a\u9009\u53d6\u5510\u5c71\u5e02\u7b2c\u4e5d\u533b\u96622017 \u5e744 \u6708\u81f32018\u5e748\u6708\u6536\u6cbb\u7684DPN\u60a3\u8005162 \u4f8b\uff0c\u91c7\u7528\u968f\u673a\u6570\u5b57\u8868\u6cd5\u5206\u4e3a\u5bf9\u7167\u7ec4\u53ca\u6cbb\u7597\u7ec4\uff0c\u540481 \u4f8b\u3002\u4e24\u7ec4\u60a3\u8005\u5747\u4e88\u5e38\u89c4DPN\u6cbb\u7597\uff0c\u5e76\u9759\u8109\u6ef4\u6ce8\u524d\u5217\u5730\u5c14\u6ce8\u5c04\u6db2\uff0c\u6cbb\u7597\u7ec4\u60a3\u8005\u52a0\u670d\u82a9\u8fde\u7fd8\u76ae\u65b9\u3002\u4e24\u7ec4\u5747\u8fde\u7eed\u6cbb\u75974 \u5468\u3002\u7ed3\u679c\uff1a\u6cbb\u7597\u7ec4\u603b\u6709\u6548\u7387\u4e3a82\uff0e72%\uff0c\u660e\u663e\u9ad8\u4e8e\u5bf9\u7167\u7ec4\u768467\uff0e90%(\u03c72<\/sup> = 8\uff0e463\uff0cP = 0\uff0e004 \uff1c0\uff0e05)\uff1b\u6cbb\u7597\u540e\uff0c\u4e24\u7ec4\u60a3\u8005\u7684\u795e\u7ecf\u4f20\u5bfc\u901f\u7387[\u5305\u62ec\u6b63\u4e2d\u795e\u7ecf\u3001\u8153\u603b\u795e\u7ecf\u7684\u8fd0\u52a8\u4f20\u5bfc\u901f\u7387( MCV)\u53ca\u6b63\u4e2d\u795e\u7ecf\u3001\u8153\u80a0\u795e\u7ecf\u7684\u611f\u89c9\u4f20\u5bfc\u901f\u7387( SCV) ]\u660e\u663e\u5feb\u4e8e\u6cbb\u7597\u524d( P \uff1c 0\uff0e 05)\uff0c\u4e14\u6cbb\u7597\u7ec4\u660e\u663e\u5feb\u4e8e\u5bf9\u7167\u7ec4( P\uff1c0\uff0e05)\uff1b\u6cbb\u7597\u540e\uff0c\u4e24\u7ec4\u60a3\u8005\u7684\u591a\u4f26\u591a\u4e34\u5e8a\u795e\u7ecf\u75c5\u53d8\u8bc4\u5206(TCSS)\u53ca\u4e2d\u533b\u8bc1\u5019\u8bc4\u5206\u5747\u660e\u663e\u4f4e\u4e8e\u6cbb\u7597\u524d\uff0c\u4e14\u6cbb\u7597\u7ec4\u660e\u663e\u4f4e\u4e8e\u5bf9\u7167\u7ec4(P\uff1c0\uff0e05)\uff1b\u4e24\u7ec4\u5747\u672a\u53d1\u73b0\u660e\u663e\u4e0d\u826f\u53cd\u5e94\u3002\u7ed3\u8bba\uff1a\u5728\u897f\u836f\u6cbb\u7597\u7684\u57fa\u7840\u4e0a\u52a0\u7528\u82a9\u8fde\u7fd8\u76ae\u65b9\u6cbb\u7597DPN\uff0c\u53ef\u6709\u6548\u7f13\u89e3\u60a3\u8005\u7684\u4e34\u5e8a\u75c7\u72b6\u52a0\u5feb\u795e\u7ecf\u4f20\u5bfc\u901f\u7387\u3002<\/p>\n

3.26 \u5927\u5934\u761f<\/p>\n

\u7a0b\u67d0\uff0c\u7537\uff0c 38 \u5c81\uff0c \u519c\u6c11\u30021982 \u5e743 \u670824 \u65e5\u8bca\u3002\u60a3\u8005\u5934\u9762\u90e8\u7ea2\u80bf\u53d1\u4eae\uff0c\u80c0\u75db \u4e24\u773c\u80bf\u6210\u4e00\u6761\u7f1d\uff0c\u89c6\u7269\u6709\u969c\u788d\u611f\uff0c\u5934\u75db\uff0c\u53d1\u70ed\u6076\u5bd2\uff0c\u53e3\u6e34\u6eb2\u9ec4\uff0c\u98df\u5c11\u80a2\u8f6f\uff0c\u820c\u8d28\u7ea2\uff0c\u82d4\u8584\u9ec4\uff0c\u8109\u6d6e\u6570\u3002\u6b64\u8bc1\u7cfb\u6625\u65e5\u5bd2\u6e29\u4e0d\u8c03\uff0c\u9633\u6c14\u53d1\u6cc4\uff0c\u98ce\u70ed\u4e0a\u6270\u9633\u7edc\u3002\u6cbb\u5b9c\u758f\u6563\u98ce\u90aa\uff0c\u6e05\u70ed\u89e3\u6bd2\uff0c\u7528\u6e05\u70ed\u89e3\u6bd2\u6c64(\u81ea\u8ba2\u65b9)\uff0c\u5904\u65b9:\u94f6\u82b130g\uff0c\u8fde\u7fd815g\uff0c\u50f5\u869510g\uff0c\u725b\u84a1\u5b5020g\uff0c\u5927\u9752\u53f620g\uff0c\u9ec4\u82a910g\uff0c\u7384\u53c220g\uff0c\u9ec4\u8fde8g\uff0c\u677f\u84dd\u683930g\uff0c\u6de1\u7af9\u53f610g\uff0c\u8584\u837710g\uff0c\u7518\u83495g\u3002\u4e0a\u65b9\u670d1 \u5242\u540e\u75c5\u51cf\uff0c \u7eed\u670d2 \u5242\u75ca\u6108\u3002<\/p>\n

3.27 \u80a0\u75c8<\/p>\n

\u7a0b\u67d0\uff0c\u7537\uff0c 34 \u5c81\uff0c \u5e72\u90e8\u30021984 \u5e747 \u670813 \u65e5\u8bca\u3002\u4e3b\u8bc9:\u9635\u53d1\u6027\u8110\u5468\u75bc\u75db\u5df21d \u3002\u60a3\u8005\u5f00\u59cb\u80c3\u8118\u75bc\u75db\uff0c \u4f34\u6076\u5fc3\u6b32\u5410\uff0c\u4ee5\u540e\u9010\u6e10\u79fb\u81f3\u8110\u5468\u75bc\u75db\uff0c\u7ecf\u7528\u897f\u836f\u6cbb\u7597\u672a\u89c1\u597d\u8f6c\uff0c \u6539\u7528\u4e2d\u533b\u4e2d\u836f\u6cbb\u7597\u3002\u73b0\u75c7:\u4f53\u578b\u7626\u957f\uff0c \u9762\u8272\u6666\u6ede\u5c11\u534e\uff0c\u8110\u5468\u5448\u9635\u53d1\u6027\u75bc\u75db\uff0c \u53f3\u4e0b\u8179\u9ea6\u6c0f\u70b9\u6709\u6307\u538b\u53cd\u8df3\u75db\u611f\uff0c\u98df\u7eb3\u5dee\uff0c\u5934\u6655\uff0c\u6076\u5fc3\uff0c \u5927\u4fbf\u4e0d\u7545\uff0c\u5c0f\u4fbf\u77ed\u8d64\uff0c \u820c\u8d28\u6de1\uff0c\u82d4\u8584\u767d\uff0c \u8109\u5f26\u7ec6\u800c\u6570\u3002\u8bca\u65ad:\u80a0\u75c8\u521d\u671f\uff0c\u75c8\u8113\u672a\u6210;\u8fa8\u8bc1:\u6b64\u8bc1\u4e43\u6e7f\u70ed\u79ef\u6ede\uff0c \u963b\u4e8e\u80a0\u80c3\uff0c\u6c14\u6ede\u8840\u51dd\uff0c \u80a0\u7edc\u4e0d\u901a\u6240\u81f4;\u6cbb\u6cd5:\u6e05\u70ed\u5bfc\u7ed3\uff0c\u7406\u6c14\u6d3b\u8840\u3002\u7528\u6e05\u70ed\u6563\u75c8\u6c64(\u81ea\u8ba2\u65b9)\uff0c \u5904\u65b9:\u94f6\u82b130g \uff0c \u8fde\u7fd820g \uff0c \u5927\u9ec410g\uff0c\u8292\u785d10g\uff0c\u6843\u4ec110g \uff0c \u6728\u999910g \uff0c \u9752\u76ae10g \uff0c\u9ec4\u67cf10g \uff0c \u8d64\u828d\u836f20g \uff0c \u7518\u83495g \u3002\u4e0a\u65b9\u670d1 \u5242\u540e\u75db\u51cf\uff0c \u7eed\u670d1 \u5242\uff0c \u8bf8\u75c7\u6089\u9664\u3002\u518d\u7528\u8c03\u548c\u80a0\u80c3\u4e4b\u534a\u590f\u6cfb\u5fc3\u6c64\u8c03\u7406\u5584\u540e\u3002<\/p>\n

3.28\u54b3\u5598<\/p>\n

\u8881\u67d0\uff0c\u5973\uff0c 32 \u5c81\uff0c \u519c\u6c11\u30021976 \u5e7411\u670814 \u65e5\u521d\u8bca\u3002\u4e3b\u75c7:\u5934\u75db\u3001\u53d1\u70ed\u3001\u54b3\u5598\u5df23d \u3002\u60a3\u8005\u81ea\u5e7c\u6709\u54ee\u5598\u5bbf\u75be\uff0c \u6bcf\u56e0\u6c14\u5019\u53d8\u5316\u800c\u53d1\u4f5c\uff0c\u53d1\u4f5c\u540e\u9700\u670d\u767e\u5598\u6216\u6c28\u8336\u78b1\u65b9\u80fd\u7f13\u89e3\uff0c \u6b64\u6b21\u53d1\u4f5c\u540e\u670d\u836f\u65e0\u6548\uff0c\u6545\u8bf7\u4e2d\u533b\u6cbb\u7597\u3002\u73b0\u75c7:\u9762\u8272\u7070\u6697\uff0c \u8116\u5b50\u7c97\u77ed\uff0c \u5934\u75db\u53d1\u70ed\uff0c \u56db\u80a2\u9178\u695a\uff0c \u53e3\u6e34\u559c\u51b7\u996e\uff0c \u5589\u95f4\u75f0\u9e23\uff0c \u54b3\u55fd\u9891\u4f5c\uff0c \u54b3\u58f0\u9ad8\u4ea2\uff0c \u54b3\u51fa\u5c11\u91cf\u6ce1\u6cab\u767d\u7c98\u75f0\uff0c \u54b3\u5267\u65f6\u6ee1\u5934\u6c57\u51fa\uff0c\u820c\u6de1\uff0c \u82d4\u767d\u5fae\u817b\uff0c\u8109\u6d6e\u6570\u800c\u6ed1\u3002\u8bc1\u5c5e\u98ce\u70ed\u72af\u80ba\uff0c \u5f15\u52a8\u5bbf\u75be\uff0c \u75f0\u70ed\u5185\u4f0f\uff0c \u706b\u70ed\u90c1\u84b8\u3002\u5b9c\u5ba3\u6563\u98ce\u90aa\uff0c \u6e05\u70ed\u5e73\u5598\u3002\u7528\u6e05\u70ed\u5e73\u5598\u6c64(\u81ea\u8ba2\u65b9)\u3002\u5904\u65b9:\u94f6\u82b120g \uff0c \u8fde\u7fd815g \uff0c \u9ebb\u9ec410g \uff0c \u674f\u4ec110g \uff0c\u77e5\u6bcd10g\uff0c \u77f3\u818f50g \uff0c \u9ec4\u82a910g \uff0c \u74dc\u848c\u4ec120g \uff0c \u8c61\u8d1d\u6bcd10g \uff0c \u7d2b\u82cf\u5b5010g \uff0c \u7518\u83495g \uff0c \u6851\u767d\u76ae20g \u3002\u4e0a\u65b9\u670d2\u5242\u70ed\u9000\u5598\u5e73\uff0c \u7ee7\u7528\u6c99\u53c2\u9ea6\u51ac\u6c64\u8c03\u7406\u800c\u6108\u3002<\/p>\n

3.29 \u6e7f\u6e29<\/p>\n

\u5434\u67d0\uff0c\u7537\uff0c 38 \u5c81\uff0c \u804c\u5de5\u30021982 \u5e749 \u670815 \u65e5\u8bca\u3002\u60a3\u8005\u6691\u6708\u7ecf\u5e38\u5192\u70ed\u884c\u8d70\uff0c \u89c1\u6709\u6c34\u51fc\u5373\u6d47\u6c34\u6d17\u8db3\uff0c\u5c1a\u611f\u51c9\u723d\u8212\u9002\uff0c \u55e3\u540e\u6e10\u611f\u80a2\u8f6f\u4e4f\u529b\uff0c\u98df\u7eb3\u4e0d\u4f73\u3002\u51e0\u5929\u540e\uff0c\u5934\u75db\u8eab\u91cd\uff0c \u6076\u5bd2\u53d1\u70ed\uff0c \u70ed\u65f6\u6709\u5fae\u6c57\u51fa\uff0c \u53e3\u6e34\u4e0d\u6b32\u996e\uff0c\u80f8\u95f7\u8118\u75de\uff0c\u820c\u767d\u82d4\u817b\uff0c \u8109\u5f26\u7ec6\u800c\u6570\u3002\u8bc1\u5c5e\u6e7f\u6e29\uff0c\u56e0\u6e7f\u8574\u70ed\u84b8\uff0c\u6c14\u673a\u88ab\u963b\u3002\u5b9c\u6e05\u70ed\u5316\u6d4a\uff0c \u5ba3\u6563\u6e7f\u90aa\uff0c\u7528\u6e05\u70ed\u5316\u6e7f\u6c64(\u81ea\u8ba2\u65b9)\u3002\u5904\u65b9:\u94f6\u82b120g \uff0c \u8fde\u7fd815g \uff0c\u85ff\u999915g\uff0c \u4f69\u517015g \uff0c\u767d\u853b\u4ec110g\uff0c \u858f\u82e1\u4ec130g \uff0c \u6ed1\u77f320g \uff0c \u9ec4\u82a910g \uff0c\u901a\u83495g\uff0c\u8377\u53f620g \u3002\u4e0a\u65b9\u670d2 \u5242\u70ed\u9000\u8eab\u8f7b\uff0c\u7eed\u670d2 \u5242\u75c5\u6108\u3002<\/p>\n

3.30\u5916\u4f24\u7600\u80bf<\/p>\n

\u590f\u67d0\uff0c\u5973\uff0c 41 \u5c81\uff0c \u519c\u6c11\u30021983 \u5e7412\u67088 \u65e5\u8bca\u3002\u60a3\u800510d \u524d\uff0c \u56e0\u4e58\u624b\u6276\u5f0f\u62d6\u62c9\u673a\u88ab\u8dcc\u4f24\u53f3\u817f\u8e1d\u90e8\uff0c\u4f24\u53e3\u5448\u73af\u5f62\uff0c \u957f\u7ea618cm \uff0cX \u7ebf\u900f\u89c6\u672a\u53d1\u73b0\u9aa8\u6298\u3002\u7ecf\u521b\u4f24\u6e05\u6d17\u7f1d\u5408\uff0c 7d \u6298\u7ebf\uff0c \u4f24\u53e3\u2161\u671f\u6108\u5408\uff0c \u4f46\u8db3\u80cc\u80bf\u80c0\uff0c \u5448\u7d2b\u84dd\u8272\uff0c \u7ecf\u897f\u533b\u8bca\u65ad\u4e3a\u8840\u6db2\u5faa\u73af\u969c\u788d\uff0c \u5efa\u8bae\u670d\u4e2d\u836f\u6cbb\u7597\u3002\u75c7\u89c1:\u9762\u9752\u800c\u6666\u6ede\uff0c \u996e\u98df\u6b63\u5e38\uff0c \u4e8c\u4fbf\u8c03\u548c\uff0c \u53f3\u8db3\u80cc\u80c0\u75db\u9ebb\u6728\uff0c \u5448\u6591\u70b9\u72b6\u6d45\u7d2b\u84dd\u8272\uff0c\u6309\u4e4b\u968f\u6307\u800c\u8d77\uff0c \u6709\u707c\u70ed\u611f\uff0c\u820c\u6de1\u82d4\u8584\u767d\uff0c \u8109\u5f26\u7ec6\u5fae\u6da9\u3002\u8bc1\u5c5e\u8840\u7600\u70ed\u90c1\uff0c\u5b9c\u6e05\u70ed\u6563\u7600\u3002\u7528\u6e05\u70ed\u6563\u7600\u6c64(\u81ea\u8ba2\u65b9)\uff0c \u5904\u65b9:\u91d1\u94f6\u82b130g \uff0c\u8fde\u7fd815g \uff0c\u6843\u4ec110g \uff0c\u7ea2\u82b110g \uff0c\u5f53\u5f5210g \uff0c\u8d64\u828d20g \uff0c\u751f\u573020g \uff0c\u9ec4\u67cf15g \uff0c\u571f\u9cd6\u866b10g \uff0c\u7518\u834910g \uff0c\u725b\u819d20g \u3002\u670d1 \u5242\u540e\u75c5\u51cf\uff0c\u7eed\u670d3 \u5242\u75ca\u6108\u3002\u540e\u968f\u8bbf\u534a\u5e74\u80a4\u8272\u6b63\u5e38\uff0c \u65e0\u4efb\u4f55\u4e0d\u9002\u3002<\/p>\n

3.31 \u98ce\u6c34\u80bf<\/p>\n

\u5f20\u67d0\uff0c\u5973\uff0c34 \u5c81\uff0c\u519c\u6c11\u30021983\u5e748 \u67085 \u65e5\u8bca\u3002\u60a3\u8005\u56e0\u611f\u5192\u5934\u8eab\u75bc\u75db\uff0c\u53d1\u70ed\u54bd\u75db\uff0c\u7b2c2d \u6668\u8d77\u5373\u89c9\u5934\u9762\u6d6e\u80bf\uff0c\u7ecf\u67d0\u536b\u751f\u9662\u8bca\u65ad\u4e3a\u6025\u6027\u80be\u5c0f\u7403\u80be\u708e\uff0c\u6cbb\u7597\u6548\u679c\u4e0d\u663e\uff0c\u6545\u6765\u6211\u9662\u95e8\u8bca\u6cbb\u7597\u3002\u73b0\u75c7:\u9762\u8272\u6666\u6697\uff0c\u904d\u8eab\u6d6e\u80bf\uff0c\u4ee5\u5934\u9762\u4e3a\u751a\uff0c\u53d1\u70ed\u6076\u5bd2\uff0c\u53e3\u6e34\u559c\u996e\uff0c\u8170\u90e8\u9178\u80c0\uff0c\u98df\u6b32\u4e0d\u4f73\uff0c\u5c0f\u4fbf\u77ed\u8d64\uff0c\u820c\u5c16\u7ea2\uff0c\u82d4\u8584\u9ec4\u5fae\u817b\uff0c \u8109\u6d6e\u5f26\u800c\u6570\u3002\u8bc1\u5c5e\u98ce\u90aa\u5916\u88ad\uff0c\u80ba\u5931\u901a\u8c03\u6c34\u9053\u800c\u504f\u70ed\u3002\u5b9c\u6e05\u70ed\u5ba3\u80ba\uff0c\u5229\u6e7f\u884c\u6c34\u3002\u7528\u6e05\u70ed\u5229\u6c34\u6c64(\u81ea\u8ba2\u65b9)\uff0c\u5904\u65b9:\u94f6\u82b120g\uff0c\u8fde\u7fd815g\uff0c\u9ec4\u82a910g\uff0c\u4e39\u76ae20g\uff0c\u9ebb\u9ec410g\uff0c\u674f\u4ec110g\uff0c\u77f3\u818f50g\uff0c\u832f\u82d3\u76ae20g\uff0c\u732a\u82d310g\uff0c\u6cfd\u6cfb10g\uff0c\u6851\u767d\u76ae20g\uff0c\u767d\u901a\u83495g\u3002\u670d2 \u5242\u540e\u6d6e\u80bf\u6e10\u6d88\uff0c\u518d\u670d3 \u5242\u6d6e\u80bf\u6d88\u5e73\uff0c\u540e\u4ee5\u9999\u7802\u516d\u541b\u5b50\u6c64\u8c03\u7406\u800c\u6108\uff0c\u968f\u8bbf\u534a\u5e74\u672a\u518d\u53d1\u3002<\/p>\n

\u738b\u67d0\u67d0\uff0c\u5973\uff0c 12\u5c81\u30023\u65e5\u524d\uff0c \u60a3\u8005\u6076\u98ce\u53d1\u70ed\uff0c \u7ee7\u5219\u9762\u76ee\u80a2\u4f53\u6d6e\u80bf\uff0c \u5598\u54b3\uff0c\u5c3f\u5c11\uff0c \u65e0\u6c57\uff0c\u820c\u6de1\u82d4\u767d\uff0c \u8109\u6d6e\u6ed1\uff0c\u5c3f\u68c0:\u86cb\u767d2+\u3002\u8bc1\u5c5e:\u98ce\u90aa\u88ad\u80ba\uff0c \u80ba\u6c14\u5931\u5ba3\uff0c \u901a\u8c03\u5931\u53f8\uff0c \u4ee5\u81f3\u6c34\u996e\u6cdb\u6ee5\u3002\u6cbb\u5b9c\u5f00\u9b3c\u95e8\uff0c\u6d01\u51c0\u5e9c\uff0c \u795b\u98ce\u5ba3\u80ba\uff0c\u901a\u8c03\u6c34\u8c03\u3002\u836f\u7528\u9ebb\u9ec43g\uff0c \u8d64\u5c0f\u8c4610g\uff0c \u674f\u4ec16g\uff0c \u6851\u76ae6g\uff0c \u8fde\u7fd810g\uff0c \u7518\u83493g\uff0c \u832f\u82d310g\uff0c \u751f\u59dc3\u7247\uff0c \u7ea2\u67a32\u679a\uff0c \u6854\u68973g\u3002\u670d\u836f3 \u5242\uff0c \u6c57\u51fa\u70ed\u9000\u3001\u5598\u5e73\u3001\u80bf\u51cf\u3001\u5c0f\u4fbf\u5229\u3002\u4e0a\u65b9\u53bb\u9ebb\u9ec4\u3001\u6854\u6897\uff0c\u52a0\u858f\u82e1\u4ec110g\uff0c \u767d\u672f8g\uff0c \u7ee7\u670d3\u5242\uff0c \u5c0f\u4fbf\u901a\u5229\uff0c \u6d6e\u80bf\u6d88\u9000\u3002\u518d\u4ee5\u9ec4\u82aa\u9632\u5df2\u6c64\u52a0\u51cf\u8c03\u7406\u800c\u6108\u3002\u6309\uff1a\u5916\u611f\u98ce\u90aa\uff0c \u80ba\u536b\u5931\u56f0\uff0c \u80ba\u6c14\u4e0d\u5ba3\uff0c \u6c14\u673a\u5931\u5e03\u5219\u6c34\u9053\u5931\u8c03\uff0c \u6c34\u90aa\u5185\u6cdb\u6d41\u6ea2\u808c\u80a4\u800c\u6210\u904d\u8eab\u6c34\u80bf\uff0c\u8fd0\u7528\u672c\u65b9\u6cbb\u7597\u98ce\u6c34\uff0c \u53ef\u5ba3\u80ba\u5229\u6c34\u901a\u8c03\u6c34\u9053\uff0c\u6700\u7ec8\u4ee5\u8fbe\u9000\u80bf\u4e4b\u6548\u3002<\/p>\n

3.32\u4e73\u817a\u589e\u751f<\/p>\n

\u5f90\u6625\u7ea2\u7b49\u89c2\u5bdf\u6d77\u85fb\u8fde\u7fd8\u65b9\u6cbb\u7597\u4e73\u817a\u589e\u751f\u7684\u4e34\u5e8a\u7597\u6548\u3002\u65b9\u6cd5\uff1a\u56de\u987e\u6027\u5206\u679086\u4f8b\u6c14\u6ede\u8840\u7600\u578b\u4e73\u817a\u589e\u751f\u60a3\u8005\u7684\u75c5\u5386\u8d44\u6599\uff0c\u6839\u636e\u6cbb\u7597\u65b9\u6cd5\u7684\u4e0d\u540c\u5206\u4e3a\u5bf9\u7167\u7ec4\u548c\u89c2\u5bdf\u7ec4\uff0c\u6bcf\u7ec443\u4f8b\u3002\u5bf9\u7167\u7ec4\u7ed9\u4e88\u4ed6\u83ab\u6614\u82ac\u7247\u5e38\u89c4\u6cbb\u7597\uff0c\u89c2\u5bdf\u7ec4\u7ed9\u4e88\u6d77\u85fb\u8fde\u7fd8\u65b9\u6cbb\u7597\u3002\u7528\u836f\u65f6\u95f4\u4e3a2\u4e2a\u6708\u7ecf\u5468\u671f\u3002\u5206\u67902\u7ec4\u7684\u4e34\u5e8a\u7597\u6548\u548c\u5b89\u5168\u6027\uff0c\u89c2\u5bdf2\u7ec4\u6cbb\u7597\u524d\u540e\u6027\u6fc0\u7d20\uff3b\u9ec4\u4f53\u751f\u6210\u6fc0\u7d20\uff08LH\uff09\u3001\u96cc\u4e8c\u9187\uff08E2\uff09\u3001\u5b55\u916e\uff08P\uff09\u3001\u50ac\u4e73\u7d20\uff08PRL\uff09\u3001\u5375\u6ce1\u523a\u6fc0\u7d20\uff08FSH\uff09\uff3d\u6c34\u5e73\u548c\u4e2d\u533b\u8bc1\u5019\u79ef\u5206\u7684\u53d8\u5316\u3002\u7ed3\u679c\uff1a\u89c2\u5bdf\u7ec4\u4e34\u5e8a\u7597\u6548\u603b\u6709\u6548\u7387\uff0895.35%\uff09\u9ad8\u4e8e\u5bf9\u7167\u7ec4\uff0883.72%\uff09\uff0c\u5dee\u5f02\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\uff08P\uff1c0.05\uff09\u30022\u7ec4FSH\u548cP\u6c34\u5e73\u5747\u8f83\u6cbb\u7597\u524d\u5347\u9ad8\uff0cLH\u3001E2\u548cPRL\u6c34\u5e73\u5747\u8f83\u6cbb\u7597\u524d\u4e0b\u964d\uff0c\u5dee\u5f02\u5747\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\uff08P\uff1c0.05\uff09\u3002\u89c2\u5bdf\u7ec4FSH\u3001P\u6c34\u5e73\u5747\u9ad8\u4e8e\u5bf9\u7167\u7ec4\uff0cLH\u3001E2\u548cPRL\u6c34\u5e73\u5747\u4f4e\u4e8e\u5bf9\u7167\u7ec4\uff0c\u5dee\u5f02\u5747\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\uff08P\uff1c0.05\uff09\u30022 \u7ec4\u80bf\u5757\u8d28\u5730\u3001\u80bf\u5757\u5927\u5c0f\u53ca\u4e73\u817a\u75bc\u75db\u79ef\u5206\u5747\u8f83\u6cbb\u7597\u524d\u4e0b\u964d\uff08P\uff1c0.05\uff09\uff0c\u89c2\u5bdf\u7ec43\u9879\u79ef\u5206\u5747\u4f4e\u4e8e\u5bf9\u7167\u7ec4\uff08P\uff1c0.05\uff09\u3002\u7ed3\u8bba\uff1a\u6d77\u85fb\u8fde\u7fd8\u65b9\u53ef\u63d0\u9ad8\u6c14\u6ede\u8840\u7600\u578b\u4e73\u817a\u589e\u751f\u60a3\u8005\u7684\u7597\u6548\uff0c\u6539\u5584\u6027\u6fc0\u7d20\u6c34\u5e73\u4e0e\u75c7\u72b6\uff0c\u7f29\u5c0f\u80bf\u5757\uff0c\u5b89\u5168\u6027\u826f\u597d\u3002\u6d77\u85fb\u8fde\u7fd8\u65b9\uff1a\u6d77\u85fb\u3001\u6606\u5e03\u3001\u6d59\u8d1d\u6bcd\u3001\u8fde\u7fd8\u3001\u5f53\u5f52\u540420 g\uff0c\u7092\u9752\u76ae\uff08\u918b\u9752\u76ae\uff0918 g\uff0c\u6cd5\u534a\u590f\u3001\u9648\u76ae\u3001\u5ddd\u828e\u540412 g\u3002\u968f\u75c7\u52a0\u51cf\uff1a\u7ecf\u524d\u4e73\u623f\u80bf\u5757\u75bc\u75db\u660e\u663e\u8005\u52a0\u7092\u9752\u76ae\u3001\u9648\u76ae\uff1b\u4f53\u5f31\u8005\u52a0\u515a\u53c2\uff1b\u4fbf\u79d8\u8005\u52a0\u719f\u5927\u9ec4\u3001\u7384\u660e\u7c89\u3002\u6bcf\u65e51\u5242\uff0c\u6c34\u714e\uff0c\u52062\u6b21\u670d\u7528\u3002\u7528\u836f\u65f6\u95f4\u4e3a2\u4e2a\u6708\u7ecf\u5468\u671f\u3002<\/p>\n

4. \u5b89\u5168\u6027<\/p>\n

\u6bd2\u6027\uff1a\u8fde\u7fd8\u6ce8\u5c04\u6db2(1\uff1a1)\u7ed9\u5c0f\u9f20\u8179\u8154\u6ce8\u5c04\u7684LD50<\/sub>\u4e3a24\uff0e85\u00b11\uff0e12 g\uff0fkg\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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